Tursiops Research Articles

Vascularization of the gastrointestinal tract of the bottlenose dolphin (Tursiops truncatus, Montagu 1821).

Odontocetes primarily rely on fish, cephalopods, and crustaceans as their main source of nutrition. In the digestive system, their polygastric complex exhibits similarities to that of their closest terrestrial relatives such as cows, sheep, and giraffes, while the entero-colic tract shares similarities with terrestrial carnivores. The morphology, caliber, and structure of the odontocete intestine are relatively constant, and, since there is no caecum, a distinction between the small and large intestine and their respective subdivisions is difficult. To address this issue, we used the intestinal vascularization pattern, specifically the course and branching of the celiac artery (CA) and the cranial and caudal mesenteric arteries (CrMA and CdMA). A series of pictures and dissections of 10 bottlenose dolphins (Tursiops truncatus) were analyzed. Additionally, we performed a cast by injecting colored polyurethane foam in both arteries and veins to measure the caliber of the arteries and clarify their monopodial or dichotomous branching. Our results showed the presence of multiple duodenal arteries (DAs) detaching from the CA. The CrMA gave origin to multiple jejunal arteries, an ileocolic artery (ICA), and, in six cases, a CdMA. In four specimens, the CdMA directly originated from the abdominal aorta. The ICA gave rise to the mesenteric ileal branches (MIB) and mesenteric anti-ileal branches and the right colic arteries (RCA) and the middle colic arteries. From the CdMA originated the left colic and cranial rectal arteries (LCA and CrRA). The measurements revealed a mixed monopodial and dichotomous branching scheme. The analysis of the arteries and their branching gave us an instrument, based on comparative anatomy, to distinguish between the different intestinal compartments. We used the midpoint of anastomoses between MIB and RCA to indicate the border between the small and the large intestine, and the midpoint of anastomoses between LCA and CrRA, to tell the colon from the rectum. This pattern suggested an elongation of the duodenum and a shortening of the colic tract that is still present in this species. These findings might be related to the crucial need to possess a long duodenal tract to digest prey ingested whole without chewing. A short aboral part is also functional to avoid gas-producing colic fermentation. The rare origin of the CdMA on the CrMA might instead be a consequence of the cranial thrust of the abdominopelvic organs related to the loss of the pelvic girdle that occurred during the evolution of cetaceans.

Impact of interactions between common bottlenose dolphins and purse-seiners in the Moroccan Mediterranean region: case study in the Al Hoceima fishing grounds

This study examines the effect of common bottlenose dolphins Tursiops truncatus on the purse-seine fishery for small pelagic fishes in the Mediterranean Sea and the economic consequences thereof. The investigation focused on the fleet registered at the port of Al Hoceima, Morocco, and used information collected from on-board observations and a semi-structured questionnaire with fishermen and ship-owners. A total of 121 dolphins were captured as bycatch during 48 fishing trips, with a mortality rate of 0.23 dolphins per fishing trip. In terms of damage to the fishing gear, the number of observed holes varied between 28 and 230 per net per incident. Though some tears were large, most were <35 cm in height. The cost of repairing the holes in the nets caused by these interactions was estimated at US$179.52 per mending event. The level of interaction between common bottlenose dolphins and the purse-seine fishery targeting small pelagic fishes is a challenge for both fishery management and dolphin conservation.

Post-mortem examination on a striped dolphin (Stenella coeruleoalba) reveals a potential fatal interaction with bottlenose dolphins (Tursiops truncatus) in Italian waters

Abstract Despite attacks of bottlenose dolphins towards conspecifics and other species of dolphins being reported worldwide, inside the Mediterranean Sea these behaviours are still considered sporadic and have been, to date, only recorded in Spain and France, mostly directed to striped dolphins. In this work, we reported the necropsy outcomes of a dead striped dolphin that suggest a fatal interaction with bottlenose dolphins inside the Italian side of the Pelagos Sanctuary, Western Ligurian Sea. Results from the post-mortem examination showed multiple, multifocal external rake marks spaced 1–1.2 cm and several internal injuries, consisting of subcutaneous and muscular haematomas and haemorrhages, multiple vertebral and rib fractures, haemothorax and lung laceration. The inter-tooth distance and all the internal findings were consistent with a fatal traumatic interaction with bottlenose dolphins as reported in similar cases in other parts of the world where the same results were found. No other significant gross and microscopic findings and concurrent significant pathogens were detected. This case highlights the existence of negative interspecific interactions in an area where they have never been reported before and adds new important information to the literature for understanding their occurrence, expanding the geographical range of observation in the western Mediterranean Sea.

The effects of range and echo-phase on range resolution in bottlenose dolphins (Tursiops truncatus) performing a successive comparison taska).

Echolocating bats and dolphins use biosonar to determine target range, but differences in range discrimination thresholds have been reported for the two species. Whether these differences represent a true difference in their sensory system capability is unknown. Here, the dolphin's range discrimination threshold as a function of absolute range and echo-phase was investigated. Using phantom echoes, the dolphins were trained to echo-inspect two simulated targets and indicate the closer target by pressing a paddle. One target was presented at a time, requiring the dolphin to hold the initial range in memory as they compared it to the second target. Range was simulated by manipulating echo-delay while the received echo levels, relative to the dolphins' clicks, were held constant. Range discrimination thresholds were determined at seven different ranges from 1.75 to 20 m. In contrast to bats, range discrimination thresholds increased from 4 to 75 cm, across the entire ranges tested. To investigate the acoustic features used more directly, discrimination thresholds were determined when the echo was given a random phase shift (±180°). Results for the constant-phase versus the random-phase echo were quantitatively similar, suggesting that dolphins used the envelope of the echo waveform to determine the difference in range.

Rings of power: evidence of mud ring feeding performed by Guiana dolphins

Abstract To capture prey more efficiently, cetaceans can display a wide range of foraging tactics to separate individual prey. Barrier feeding tactics are performed to restrict prey movements, using natural and non-natural barriers and some species can even create barriers with their own bodies. Mud ring feeding has been observed in bottlenose dolphins in Florida Bay and in Chetumal-Corozal Bay, where ring-maker dolphins create ring-shaped mud plumes to encircle fish schools. Here, we document for the first time Guiana dolphins performing the mud ring feeding behaviour in the Cananéia estuarine system, in the southern portion of the state of São Paulo, Brazil. A total of 11 dolphins were recorded in four expeditions through aerial footage engaging in the behaviour. These findings expand our knowledge about the behavioural plasticity of the species and builds upon existing records of mud ring feeding behaviour in cetaceans.

Evaluation of plasma atrial natriuretic peptide concentration in healthy bottlenose dolphins (Tursiops truncates).

There are currently no standard methods for diagnosing cardiac diseases in dolphins. These diseases may consequently be overlooked and go undiagnosed. The presence and severity of cardiac diseases in humans can be determined using blood tests. Atrial natriuretic peptide (ANP) used in human cardiac examinations has low species specificity. There have already been reports of homology between dolphin and human ANP; however, its potential for clinical application in dolphins has not been tested. This study was conducted to establish a reference for ANP levels in healthy bottlenose dolphins. Healthy bottlenose dolphins (seven females; estimated to be 7-30 years of age) at an aquarium in Japan were sampled. Each animal was tested for ANP at least three times, and the mean value and standard deviation were calculated to be 43.4 ± 19.2 pg/mL. In humans, patients with high plasma ANP levels have a poor prognosis. In veterinary medicine, cutoff values for the diagnosis of mitral regurgitation and heart failure in dogs have been established and used to predict prognosis. The results of the present study may contribute to the health management of bottlenose dolphins, particularly in the early detection and treatment of cardiac diseases.

Correction: Modified artificial rabbits optimization combined with bottlenose dolphin optimizer in feature selection of network intrusion detection

Correction: Modified artificial rabbits optimization combined with bottlenose dolphin optimizer in feature selection of network intrusion detection

Whales and dolphins of the Adriatic Sea

The Adriatic Sea is one of the Mediterranean areas most exposed to cumulative human stressors. This semienclosed and largely shallow basin has been subjected to intensive exploitation and destructive fishing practices for decades, resulting in biodiversity decline and poor ecosystem health. Diversity of cetaceans is lower than in other parts of the Mediterranean, and the extant dolphins and whales have been facing threats that include the combined effects of habitat loss and degradation, prey depletion, incidental mortality and injury caused by fisheries, anthropogenic noise, chemical contamination and climate change. Here, we report information for the nine cetacean species known to occur in the basin (classified as either regular, visitor or vagrant), plus three species characterized by a single record. For these species, we review evidence from field research and other studies – with a bias towards the common bottlenose dolphin Tursiops truncatus (by far the most intensively-studied cetacean in this area). We also describe and characterize the main threats to cetaceans in the Adriatic, relying on recent literature as well as historical information that helps frame the present status of cetaceans in the context of past human impacts (particularly the extensive killing campaigns conducted until the 1960s). Finally, we provide management recommendations to inform and guide the action that must be taken in compliance with extant legislation, marine conservation directives and international commitments to protect marine biodiversity.

Finite mixtures in capture-recapture surveys for modeling residency patterns in marine wildlife populations.

This work aims to show how prior knowledge about the structure of a heterogeneous animal population can be leveraged to improve the abundance estimation from capture-recapture survey data. We combine the Open Jolly-Seber model with finite mixtures and propose a parsimonious specification tailored to the residency patterns of the common bottlenose dolphin. We employ a Bayesian framework for our inference, discussing the appropriate choice of priors to mitigate label-switching and nonidentifiability issues, commonly associated with finite mixture models. We conduct a series of simulation experiments to illustrate the competitive advantage of our proposal over less specific alternatives. The proposed approach is applied to data collected on the common bottlenose dolphin population inhabiting the Tiber River estuary (Mediterranean Sea). Our results provide novel insights into this population's size and structure, shedding light on some of the ecological processes governing itsdynamics.

Modified artificial rabbits optimization combined with bottlenose dolphin optimizer in feature selection of network intrusion detection

&lt;abstract&gt; &lt;p&gt;For the feature selection of network intrusion detection, the issue of numerous redundant features arises, posing challenges in enhancing detection accuracy and adversely affecting overall performance to some extent. Artificial rabbits optimization (ARO) is capable of reducing redundant features and can be applied for the feature selection of network intrusion detection. The ARO exhibits a slow iteration speed in the exploration phase of the population and is prone to an iterative stagnation condition in the exploitation phase, which hinders its ability to deliver outstanding performance in the aforementioned problems. First, to enhance the global exploration capabilities further, the thinking of ARO incorporates the mud ring feeding strategy from the bottlenose dolphin optimizer (BDO). Simultaneously, for adjusting the exploration and exploitation phases, the ARO employs an adaptive switching mechanism. Second, to avoid the original algorithm getting trapped in the local optimum during the local exploitation phase, the levy flight strategy is adopted. Lastly, the dynamic lens-imaging strategy is introduced to enhance population variety and facilitate escape from the local optimum. Then, this paper proposes a modified ARO, namely LBARO, a hybrid algorithm that combines BDO and ARO, for feature selection in the network intrusion detection model. The LBARO is first empirically evaluated to comprehensively demonstrate the superiority of the proposed algorithm, using 8 benchmark test functions and 4 UCI datasets. Subsequently, the LBARO is integrated into the feature selection process of the network intrusion detection model for classification experimental validation. This integration is validated utilizing the NSL-KDD, UNSW NB-15, and InSDN datasets, respectively. Experimental results indicate that the proposed model based on LBARO successfully reduces redundant characteristics while enhancing the classification capabilities of network intrusion detection.&lt;/p&gt; &lt;/abstract&gt;

Tying up loose ends together: Cetaceans, maritime traffic and spatial management tools in the Strait of Gibraltar

Abstract The transboundary area of the Strait of Gibraltar is home to seven protected cetacean species that are threatened by high intensity of maritime traffic. More comprehensive knowledge of cetaceans and maritime traffic is required, together with analyses of legislations, strategies and policies. This study quantitatively investigates cetacean distribution and maritime traffic intensity and, for the species of community interest bottlenose dolphin, habitat suitability. Results are qualitatively discussed considering the overlap of cetacean hot spots with different maritime activities and the consistency of spatial conservation management measures in force. The Fixed Line Transect Mediterranean Monitoring Network protocols were followed for 59 visual surveys using ferries as observation platforms for monitoring cetaceans and maritime traffic. Surveys were carried out along the transects between Algeciras and Ceuta and between Algeciras and Tanger‐Med, in 2018 and 2019. 264 cetacean sightings, including seven different species and four near‐miss collision events (involving pilot, sperm and fin whales), were reported. Monitoring cetaceans from ferries in the Strait provided insights into cetacean distribution and maritime traffic, enabling the identification of cetacean hot spots, suitable habitats and maritime traffic high‐risk zones. A transboundary management effort is required, together with an adaptive approach for protecting highly mobile species such as cetaceans. Proposals include a long‐term cetacean monitoring program carried out by dedicated observers on board ferries as a cost‐effective methodology and mandatory training for crew members, to increase cetacean knowledge and reduce collision risk. The designation of an international temporal or, in some zones, permanent speed reduction area (i.e., Cetacean Critical Navigation Zone, with a maximum speed of 13 knots) and of a micro‐sanctuary with a seasonal no‐take zone in the Bay between Algeciras and Gibraltar, together with international surveillance, are recommended measures for the enhancement of conservation efforts in the Strait.

Vocal signatures affected by population identity and environmental sound levels.

Passive acoustic monitoring has improved our understanding of vocalizing organisms in remote habitats and during all weather conditions. Many vocally active species are highly mobile, and their populations overlap. However, distinct vocalizations allow the tracking and discrimination of individuals or populations. Using signature whistles, the individually distinct calls of bottlenose dolphins, we calculated a minimum abundance of individuals, characterized and compared signature whistles from five locations, and determined reoccurrences of individuals throughout the Mid-Atlantic Bight and Chesapeake Bay, USA. We identified 1,888 signature whistles in which the duration, number of extrema, start, end, and minimum frequencies of signature whistles varied significantly by site. All characteristics of signature whistles were deemed important for determining from which site the whistle originated and due to the distinct signature whistle characteristics and lack of spatial mixing of the dolphins detected at the Offshore site, we suspect that these dolphins are of a different population than those at the Coastal and Bay sites. Signature whistles were also found to be shorter when sound levels were higher. Using only the passively recorded vocalizations of this marine top predator, we obtained information about its population and how it is affected by ambient sound levels, which will increase as offshore wind energy is developed. In this rapidly developing area, these calls offer critical management insights for this protected species.

Effects of a simple method for determining the time of insemination and different methods on artificial insemination in common bottlenose dolphins (Tursiops truncatus).

In this study, we examined the usefulness of a simpler and more feasible method for determining the optimal timing of artificial insemination and the conditions for its success in six female common bottlenose dolphins. Pregnancy was successfully achieved in five dolphins by performing intrauterine insemination, using chilled semen stored for less than 3 days or frozen semen within 24 hr of exhibiting a peak serum estradiol (E2) level of 100 pg/mL or higher or on the day with a serum E2 level of approximately 100 pg/mL, measured with a simple measuring device. We concluded that the determining the optimal timing of intrauterine insemination by measuring serum E2 levels is a simpler and more useful method compared with the conventional approach.

Establishment and characterization of a novel lung cell line derived from the common bottlenose dolphin.

Cetaceans are specialized marine mammals with a unique respiratory system adapted for diving behavior. Furthermore, respiratory diseases are commonly observed in these mammals. Nevertheless, much of their respiratory physiology remains unknown due to the limited supply and poor quality of their biological samples for research. In this study, we established a novel lung cell line, dLu, derived from the common bottlenose dolphin (Tursiops truncatus), which can prove useful in cetacean research, including for understanding the pathogenesis of respiratory diseases in cetaceans. The cells were cultured in a simple medium consisting of Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. The morphology of the cells was fibroblast-like. dLu was produced by transfecting the simian virus 40 large T antigen into primary cultured cells. Although dLu exhibited approximately 80 cell divisions, it was unable to achieve complete immortalization, as the cells stopped proliferating beyond this number. dLu cells expressed toll-like receptor 3 but not toll-like receptor 4. Immunostimulation with poly(I:C) altered the gene expressions of interferon beta 1 and tumor necrosis factor alpha in dLu cells. In summary, dLu established in this study is a novel cetacean cell resource that can be easily cultured and is a useful in vitro tool in cetacean research, particularly for studying host immune responses in the lungs.

Electric boat underwater radiated noise and its potential impact on species of conservation interest

Electric boats are thought to be noiseless, but in-situ measurements are generally rare. The Underwater Radiated Noise (URN) of 8-m Trimaran Pontoon Boat with two outboard electric engines was measured in the Miramare Marine Protected Area (Trieste, Italy), together with the URN of a fibreglass 5-m boat, with a outboard gasoline engine, for comparisons. International standards and guidelines for shallow waters were considered. URN were provided in one-third octave band and in narrow band spectra. The electric boat produced a low input of underwater noise at low frequencies. Given a low-frequency hearing sensitivity, the listening space reduction (LSR) was lower when generated by the electric than by combustion engine boat for the brown meagre, a local Teleost fish. No difference was found for the bottlenose dolphin LSR although continuous, tonal, high frequency components generated by the electric boat are expected to be highly detrimental for the bottlenose dolphin.

Examining the effect of salinity on dolphin mortality using Lagrangian particle tracking in a hydrodynamic model

Numerous dolphins are found dead on beaches and waterways of the Gulf of Mexico and Mississippi Sound every year. In 2019 an unusual mortality event (UME) occurred when 337 deceased bottlenose dolphins were stranded between Louisiana, Mississippi, Alabama, and Florida. According to NOAA, based on observations of skin lesions or distinct ulcerative dermatitis and other internal pathologic findings and the environmental conditions during that period of time, the identified cause of this UME was determined to be protracted exposure to low salinity waters. Dolphin carcasses are often found stranded on beaches days after they die. Consequently, their initial place of death is unknown. In this study, an existing 2D hydrodynamic model (EFDC+ 11.2) of the Mississippi Sound simulated by Armandei et al. (2021) was used to track dolphin carcass movement and to simulate salinity at the time and location of each predicted dolphin's death. Particles within the Lagrangian particle tracking module were used to represent the movement of 19 dolphin carcasses. A large number of particles (virtual dolphin carcasses) were seeded throughout the model domain and tracked for five days. These results were used to hindcast each dolphin's original location of death. The results indicate that the most likely place of death for 12 dolphins stranded on the beaches of Mississippi, along with the two found on Ship and Horn Islands, was west of their stranding location., For both of the dolphins stranded on Dauphin Island, the most likely place of death was in the Mobile Bay area north of where they were found. The remaining three dolphins found dead on barrier islands, most likely originally died in close proximity to their stranding locations. The average simulated salinity of all the dolphins' most probable original place of death was below five except for two cases. These results are compared to the salinity of the Mississippi Sound during Bonnet Carré spillway opening and non-opening dates. The results highlight the significant impact of the spillway's opening on the reduction of salinity and its association with dolphin mortality.

Global avian influenza situation (2019–2022). Host range expansion asevidence of high pathogenicity avian influenza virus evolution

High pathogenicity avian influenza has a significant negative impact on poultry farming, international trade and health of wild bird populations, therefore the infection requires the utmost attention of the entire international community. The article investigates the evolutionary and epidemic processes observed in recent years in many countries of the world where avian influenza outbreaks occur; describes the ways of the infection spread; the prevalence of the virus types for the last several years, as well as the expansion of the host range, including among representatives of the Mammalia class. The change in the ratio between the virus types starting from 2020, when H5N8 subtype was responsible for the overwhelming number of the disease outbreaks reported, until 2022, when an obvious predominance of H5N1 subtype was detected is demonstrated. A noticeable expansion of the disease-affected areas in Central and South America, the influence of migration, anthropogenic and other factors on influenza spread are highlighted. The conditions facilitating the occurrence of the infection outbreaks affecting mammals, wild animals and livestock, zoo and companion animals are described. Cases of mammals’ infection on the North American and Eurasian continents in zoos, nature parks, backyards and fur farms, asa rule, coincide in time with the infection outbreaks in waterfowl populations. The WAHIS data were analyzed and the high ability of the virus to spillover from birds to mammals, such as martens (minks, otters, ferrets, badgers), cats (domestic cats, cougars, leopards, lynxes), pinnipeds (common seals, grey seals), bears (brown, grizzly, American black), bottlenose dolphins, skunks, foxes, opossums, raccoons was demonstrated. Changes in the habitats of both migratory birds and mammals, including due to some human economic activities, add an ecological and urban component to the complex task of the control and prevention of the epidemic, also posing a potential threat to humans.

Evaluation of fatty acids and carnitine as biomarkers of PFOS exposure in biota (fish and dolphin) from Galveston Bay and the northwestern Gulf of Mexico

Perfluorooctane sulfonate (PFOS) is a ubiquitous pollutant that elicits a wide range of toxic effects in exposed biota. Coastal zones in highly urbanized or industrial areas are particularly vulnerable to PFOS pollution. At present, information is lacking on biomarkers to assess PFOS effects on aquatic wildlife. This study investigated the efficacy of l-carnitine (or carnitine) and fatty acids as biomarkers of PFOS exposure in aquatic biota. The levels of PFOS, total and free carnitine, and 24 fatty acids (measured as fatty acid methyl esters or FAMEs) were measured in the liver, and muscle or blubber, of fish and dolphins sampled from Galveston Bay and the northern Gulf of Mexico (nGoM). Overall, bottlenose dolphins (Tursiops truncatus) had the highest hepatic PFOS levels. Galveston Bay fish, gafftopsail catfish (Bagre marinus), red drum (Sciaenops ocellatus), and spotted seatrout (Cynoscion nebulosus), had hepatic PFOS levels ∼8-13× higher than nGoM pelagic fish species, red snapper (Lutjanus campechanus) and yellowfin tuna (Thunnus albacares). The multivariate analysis of PFOS liver body-burdens and biomarkers found carnitine to be a more modal biomarker of PFOS exposure than FAMEs. Significant positive correlation of hepatic PFOS levels with total carnitine was evident for biota from Galveston Bay (fish only), and a significant correlation between PFOS and total and free carnitine was evident for biota from the nGoM (fish and dolphins). Given the essential role of carnitine in mediating fatty acid β-oxidation, our results suggest carnitine to be a likely candidate biomarker of environmental PFOS exposure and indicative of potential dyslipidemia effects.

Temporal patterns in the soundscape of the port area in an urban estuary

Soundscape ecology, facilitated by the collection of environmental sounds, enables the determination of the area used by specific species, estimation of population abundance, verification of habitat health, and assistance in management and monitoring processes. Estuaries are key environments in the life cycle of many species. As coastal ecosystems, they face potential impacts by human activities. This study aims to characterize the soundscape of the Patos Lagoon estuary (PLE), Brazil, examining its temporal and seasonal variations. Additionally, it seeks to investigate the influence of temperature and salinity on sound pressure levels (SPL) across three different frequency bands throughout the year. For data collection, two autonomous devices - a SoundTrap digital recorder and an F-POD sampler - were used. The data collection spanned from September 2020 to August 2021 and covered one consecutive week per season. The analysis of audio files was performed manually using Raven software. Sound emissions by Lahille's bottlenose dolphin (Tursiops truncatus gephyreus) and fish were observed throughout the sampling period, with peak detections occurring during the summer and spring, respectively. Boat noise was detected throughout the year but more intensely in the summer. Generalized linear models (GLMs) revealed that, for the three SPL frequency bands, the season significantly influenced the composition of the PLE soundscape, with positive fluctuations in the warmer seasons (summer > spring > autumn > winter). Salinity, in turn, showed a slightly negative relationship with the SPL values in the three models, suggesting a decrease in frequencies with increasing salinity. Our results also indicate that the low-frequency SPL, originating mostly from various sources of anthropogenic noise, constitutes the main component of the PLE soundscape. In conclusion, our study provides novel insights into the temporal patterns of the soundscape in an urban estuary, including biotic and anthropogenic sounds, and their relationships with selected environmental variables.

Towards a sustainable fisher-dolphin coexistence: Understanding depredation, assessing economic damage and evaluating management options

Finding solutions for a sustainable coexistence between wildlife and humans is considered among the most challenging environmental management issues for scientists, conservationists, managers, and stockholders world-wide. Depredation by the common bottlenose dolphin (Tursiops truncatus) on small scale fisheries has increased in the recent years, leading to a growing conflict in many areas of the Mediterranean Sea and pressing for urgent management solutions. This study aims at developing a management framework for a sustainable coexistence between fishers and dolphins in Sardinia (Mediterranean Sea). Relying on the combination of different approaches (field study, literature review and Multi Criteria Decision Analysis), the scientific evidence necessary for understanding dolphin depredation were updated and improved, the related economic damage was calculated, and different management options were identified and evaluated by several stakeholder groups to support the decision-making process. Averaging for all investigated net types (gillnet and trammel net), a depredation frequency of 53% was found, the highest values ever found in both Sardinia and many other Mediterranean sites. Depredation probability was influenced by different factors, such as net type, fishing operation duration, depth of the fishing site and period. The estimated economic damage due to depredation ranges on average between 6492 and 11,925 euro per year and depends on the type of fishing net. The results from the field study, the literature review and the stakeholder involvement allowed us to define the most plausible and shared management options, identifying a framework for assessing and managing the conflict between fishers and dolphins for the creation of a more sustainable vision for the future.