In this paper, we prove the real-rootedness of a family of generalized Narayana polynomials which arose in the study of the infinite log-concavity of the Boros-Moll polynomials. We establish certain recurrence relations for these Narayana polynomials, from which we derive the real-rootedness. In order to prove the real-rootedness, we use a sufficient condition due to Liu and Wang to determine whether two polynomials have interlaced zeros. The recurrence relations are verified with the help of the $Mathematica$ package $HolonomicFunctions$.