Here, we present the optical detection of chloride ions with co-extraction based anion-selective optodes containing a BODIPY derivative (BDP-OH) and the ionophore In(OEP)Cl. Spectroscopic studies suggested that BDP-OH and In(OEP)Cl formed an adduct BDP-O-In(OEP), which was converted to BDP-OH and In(OEP)Cl upon increasing sample Cl− concentration, and induced signal changes in both fluorescence and absorbance modes. The method was evaluated in polystyrene-graft-poly(ethylene oxide) (PS-PEO) nanospheres (ca. 40 nm in diameter) and on cellulose paper. In contrast to Cl− probes based on fluorescence quenching, the nanospheres exhibited turn-on fluorescence and ratiometric absorbance responses to a tunable Cl− concentration range (10 μM–1 M). Through fluorescence microscopy, the nanospheres were able to respond to Cl− concentration changes in HeLa cells. Cellulose paper-based Cl− optodes with colorimetric response were successfully used to measure Cl− in artificial sweat, providing a potential analytical tool for clinical diagnosis of cystic fibrosis.