BackgroundAvailability of good water source is essential to every community in other to allay the risk of water-borne infection and diseases. In this study, the isolation of parasites and rate of parasitic contamination from different water sources were evaluated in Akure, Nigeria. A total of 816 water samples were collected from wells, streams, boreholes, and rain in the study areas. The samples were examined using calcium carbonate floatation and stained microscopic methods. Also, parameters (temperature, pH, and turbidity) of the different water sources were measured.ResultsOf all the water sources, stream water samples recorded the highest prevalence (45%), followed by well water (24%), rain water (21%), and borehole water having the least prevalence of 10% with significant difference. Different developmental stages of both protozoan and helminthic parasites were isolated from all the sampled water sources. Protozoa parasites isolated were Cryptosporidium parvum, Gardia spp., and Entamoeba histolytica with prevalence of 33.2, 19.9, and 13.0% respectively. Dracunculus medinensis and Enterobius vermicularis with prevalence of 10.3% and 4.0% respectively were helminthes recovered. It was observed that the occurrence of the parasites in the water sources was affected by the water parameters (temperature, pH, and turbidity). All the water sources harbored at least one parasite except for the borehole water sources in Akure North in which no parasite was isolated.ConclusionHigh parasitic contamination of these water sources imply that water should be treated before consumption to reduce the risk of contracting water-borne disease. Also, government at the local level should provide borehole water system for the communities.