Molecular phylogeny based on complete plastid genomes indicates that a previously unknown but locally common bamboo from limestone areas near the border of China and Vietnam is a member of a strongly supported monophyletic Ampelocalamus (Poaceae: Bambusoideae). Hence, Ampelocalamus sinovietnamensis sp. nov. is described. It is similar to A. actinotrichus in having culm leaf sheaths with long oral setae and relatively large foliage leaf blades that are up to 4 cm wide, but is easily distinguished from the latter by its mid‐culm branch complement with one central dominant branch elongating to reiterate the culm and one to three lateral slender branches, ovate‐elliptic culm buds, glabrous culm internodes, culm leaves with densely strigose and brown papillate‐hirsute sheaths that are much longer than the internodes and shortly ciliolate inner ligules with 0.2–0.3 mm long ciliae, and foliage leaves with glabrous blades, more or less strigose sheaths and shortly ciliolate inner ligules with ca 0.1 mm long ciliae.