Introduction: Some professions are somewhat more exposed to stress and their working conditions are a subject of research for a long time, along with the occurrence of stress and its impact on their health. Research on professional stress among employees at workplaces with special health risks is of special importance. The aim of the paper is to point out the methodological approach to the research of professional stress among workers employed in workplaces with increased health risk. Methods: We analyzed the data and literature in the field of occupational medicine, internal medicine, psychology, neuropsychiatry, public health, quality of life and health statistics in the part relating to the impact of stress on human health, with particular emphasis on the methodology to be applied in pursuance these investigations. Results and discussion: Law on safety and health at work ('Official Gazette of the Republic of Serbia', No 101/05) and Rule book on the manner and procedure of risk assessment at the workplace and working environment ('Official Gazette of the Republic of Serbia', No 72/06) still do not have a measurement of the level of stress at work among the existing methods for risk assessment. The fact is that concern for the mental health of employees is not a luxury but a professional obligation of employers. Methods used to evaluate stress at work can be subjective, objective and intermediate. Subjective methods are questionnaires or self-evaluation, where workers evaluate the level of their own problems, demands and the weight of their work, interpersonal relations, etc. Objective methods include determining the level of cortisol and catecholamines in urine. Intermediate methods are partly subjective and include talking with other workers, the analysis of sick leaves, analysis of workplaces with increased risk, productivity analysis, injuries at work, the most common diseases among a group of employees, an interview with superiors, company management and others. To assess the subjective experience of stress in the workplace and identify stressors, many types of questionnaires are being used. In all studies, it is necessary to take basic data about the respondents, and appropriate questionnaires already designed, so-called Respondents' Basic Demographic Indicators Questionnaire. In addition, it is necessary to use the Work Ability Index Questionnaire, which is a good instrument for the assessment of work capacity. IRS Questionnaire is used for assessing the sources of stress at work. General Health Questionnsire - GHQ (Goldberg, D, Williams, P, 1991) measures the feeling of anxiety, depression, inability to defend, insomnia based on anxiety, lack of self-confidence and self-esteem and other symptoms of psychological disorders functioning. Copenhagen Burnout Inventory (CBI) is a questionnaire that includes tests relating to personal burnout, burnout at work and client burnout. Study is based on modern methods of statistical data processing. The use of these questionnaires provides a comparison of our results with the results of the research of other domestic and foreign researchers. Conclusion: It is necessary that specific working conditions, the occurrence of stress and its impact on health become a subject of more extensive research, with specially designed questionnaires. Not all questionnaires can be used in any working population, because the factors that cause stress differ in type, scale and duration. It is necessary to adapt the set of questionnaires to respective type of activity, so that those that provide accurate results when assessing stress in certain professions can be used next to the general questionnaires. Usage of the appropriate sets of questionnaires in research of professional stress among workers employed in workplaces with increased health risk enables getting valid results and allows comparison of the obtained results with the results of relevant domestic and foreign research.