Bone Rods Research Articles

HISTOLOGICAL AND MORPHOMETRIC STUDIES OF BONE TISSUE AUTOGRAFTS FROM INTRAORAL AND EXTRAORAL DONOR ZONES

Major research carried out in modern dentistry and maxillofacial surgery is commonly focused on long-term and stable results for implant rehabilitation of jaw defects and deformities in patients with bone and soft tissue losses at the site of planned implantation. Various osteoplastic materials, donor or the patient’s own bone, tissue-engineering and cellular products are common in clinical medicine, since guided regeneration can help not only recover the volume of bone tissue once lost, yet also ensure an outcome acceptable from both functional and aesthetic view. Guided bone regeneration employs methods of regenerative medicine and tissue engineering, with transplantation of native bone tissue and cell transplants, as well as various osteoplastic materials with osteoinductive and osteoconductive properties. In order to compare the structure of intact bone tissue obtained from intraoral and extraoral donor zones, histological and morphometric studies of bone autografts in 4 certified male cadavers with intact dentition were carried out. The results of histological studies in bone tissue autografts from intraoral and extraoral donor zones revealed that the highest density of bone rods could be observed in biopsies taken from the mandible outer oblique line area and chin symphysis. Whereas the lowest rate of the said factor we observed in the biopsies obtained from the iliac crest area. The highest rate of the inter-rod girder space was found in the area of the iliac crest, and the minimal – in biopsies from the parietal bone. The highest bone vascularization level was identified at the iliac crest and the parietal bone, while its lowest levels were seen in biopsies taken from the area of the mandible outer oblique line. The density of cellular elements was found to be highest at the iliac crest. The lowest density level was registered in biopsies from the area of the mandible outer oblique line. The high rates of cellular elements in autografts from the iliac crest area can be accounted for by the predominance of spongy substance over cortical, while in bone biopsies taken from the mandible outer oblique line, chin symphysis, maxillary tuberosity and parietal bone, the share of cellular elements failed to exceed 27% within the total data set. This points at the predominance of cortical rather than spongy substance. In reconstructive bone plastic surgery for augmentation of the maxillary alveolar process and the mandible alveolar part with significant atrophy, it is reasonable to use autogenous transplants taken from intraoral donor zones. The autogenous transplants have cortical morphology and embryological origin similar to the jaw bones. Bone blocks from the donor zones of the mandible outer oblique line, the chin symphysis and the maxillary tuberosity have the highest density of the bone cortical substance and the duly sufficient amount of spongy substance.

Morphological and morphometric changes of bone tissue in patients with osteoporosis and osteomalation

Bone tissue was studied in 56 postmenopausal women (mean age 62.30 ± 2.74 years), of which 46 patients who worked in unfavorable working conditions had a decreased bone mineral density (BMD) (osteoporosis (OP) — in 31 women, osteomalacia (OM) — in 13); 10 women had no metabolic changes in bone tissue (BT). A BT scan fragment was obtained during surgery for a fracture of the femoral neck. Non-decalcified QD sections were prepared, the functional activity of the QD cell nuclei was determined using the method of differential staining of nuclei with different functional activity. Morphological changes in OP and OM have both common features and differences. The common is the thinning of the bone rods, the expansion of the canals of osteons, the presence of cell-free areas, and cell-free lacunae. In contrast to OP, OM presents with the thickness and area of the osteoid increase, a less pronounced decrease in oxyphyllin matrix, a higher functional activity of BT cells. A decrease in BMD and the occurrence of low-energy fractures may result not only from OP but also OM. When prescribing treatment, it is necessary to carry out diffe-rential diagnostic measures that determine the cause of the decrease in bone mass.

Morphometric characteristics of bone tissue in the treatment of experimental tuberculous osteitis with anti-tuberculosis drugs and selective inhibitors of bisphosphonates

Introduction. The effect of bisphosphonates on bone recovery after surgical treatment of osteitis has not been fully understood. Aim: To study morphometric features of bone restoration when bisphosphonates are used after the surgical treatment of experimental tuberculous oste-itis. Material and methods. Experimental model of TB osteitis of distal femur was produced in 21 sexually ma-ture chinchilla male rabbits of distal epimetaphys of fem-oral bone. After resection of the locus and osteoplasty, 3 study arms have been formed depending on the medica-tion therapy: anti-TB drugs only, anti-TB drugs + bisphos-phonates (BF) and BF only. After the end of experiment, 3 and 5 months after surgery, material was harvested in autopsy and histological sections 3–5 microns thick were morphometrically studied on the basis of digital images of the preparations. Results. 3 months after the opera-tion, a greater thickness of the bone rods was noted in the group of rabbits treated with anti-TB drugs + BF, after 6 months — in those treated with BF only (p<0.001). In all the studied groups, relief of specific TB inflammation was achieved in 50–75% of cases 6 months after surgical treatment. Conclusions: The use of BF in the treatment of experimental tuberculous osteitis does not impact the frequency of detection and the area of specific inflamma-tion, while providing the greatest thickness of the bone rods at 3 and 6 months after surgical treatment.

To haft and to hold: Evidence for the hafting of Clovis fluted points

Clovis fluted points vary considerably in technology and morphology, but also share a set of attributes, the most diagnostic of which are the flute scars, the remnants of the flake removals from the basal region that travelled up towards the tip. Fluting on Clovis and Clovis-like points generally extends no further than a third of the way up the face of the point. Finished points are usually ground smooth along the base and lower edges, suggesting facilitation of the hafting (attachment) to a wooden shaft or handle by way of an ivory or bone socket. The points may have been hafted directly to a main-shaft and used as a thrusting spear during close encounter attacks, or in the hand as knife or butchery tool. Alternatively, an intermediary shaft, or foreshaft may have been used to secure the point. The suggestion of foreshafts being used by Clovis hunters received support after the discovery of bone rods in association with mammoth remains and Clovis points at the type site at Blackwater Draw, New Mexico in 1936. Several other Clovis-aged sites across North America have yielded ivory and beveled rods that have also been associated with foreshafts and the hafting of Clovis points. Scratches that are present on a couple of Clovis points made on varieties of obsidian, have been identified as being “hafting abrasion” evidence, this roughening of the surface would have helped in securing the point into the shaft or socket. In one example from the Hoyt site in Oregon, remains of a “pitch” or hafting adhesive was discovered in the abrasions in the fluted area of the point.

Mammoth Ivory Rods in Eastern Beringia: Earliest in North America

The Holzman archaeological site, located along Shaw Creek in interior Alaska, contained two mammoth ivory rods, of which one is bi-beveled, within a stratigraphically sealed cultural context. Dated 13,600–13,300 cal BP, these are the earliest known examples of osseous rod technology in the Americas. Beveled ivory, antler, and bone rods and points share technological similarities between Upper Paleolithic Europe, Asia, eastern Beringia, and the Clovis tradition of North America and are important tool types in understanding the late Pleistocene dispersal of modern humans. The Holzman finds are comparable to well-known Clovis tradition artifacts from Anzick (Montana), Blackwater Draw (New Mexico), East Wenatchee (Washington), and Sherman Cave (Ohio). We describe these tools in the broader context of late Pleistocene osseous technology with implications for acquisition and use of mammoth ivory in eastern Beringia and beyond.

A rediscovered beveled osseous rod: Clarification of the archaeological record

Elongated osseous implements, often referred to as osseous or bone rods, are a defining characteristic of Early Paleoindian cultures of North America. A rod found in the Lindenmeier collection at the Denver Museum of Nature & Science, was radiocarbon dated and the results published several years ago, demonstrating its Mid Paleoindian association. The specimen highlighted the importance of museum collections for furthering our knowledge of rare items of the past. Subsequently, the “Lindenmeier” rod was found to be from a different location, the Hell Gap site. In this paper we trace the rod’s history to correct the archaeological record and discuss its place within variations among osseous rods.

Stress State of a Threaded Joint in a Dental Implant–Bone System*

The load distribution over the turns of an implant–bone threaded joint is studied. Such a joint is modeled by a rod structure in which the functional axial force is transferred from the compressed-rod–implant system to the stretched-rod–bone system through thread turns. The turns are modeled by cantilever beams attached to the implant and bone rods. Differential equation describing the intensity of distribution of the axial force is derived in a closed form. The proposed approach to determining the distribution of the load over the thread of the implant–bone joint can be used to determine the stress state of the joint, improve its design, and increase the service life of dental prostheses.

Setting the Stage: The Late Pleistocene Colonization of North America

The timing of human entrance into North America has been a topic of debate that dates back to the late 19th century. Central to the modern discussion is not whether late Pleistocene-age populations were present on the continent, but the timing of their arrival. Key to the debate is the age of tools—bone rods, large prismatic stone blades, and bifacially chipped and fluted stone weapon tips—often found associated with the remains of late Pleistocene fauna. For decades, it was assumed that this techno-complex—termed “Clovis”—was left by the first humans in North America, who, by 11,000–12,000 years ago, made their way eastward across the Bering Land Bridge, or Beringia, and then turned south through a corridor that ran between the Cordilleran and Laurentide ice sheets, which blanketed the northern half of the continent. That scenario has been challenged by more-recent archaeological and archaeogenetic data that suggest populations entered North America as much as 15,300–14,300 years ago and moved south along the Pacific Coast and/or through the ice-free corridor, which apparently was open several thousand years earlier than initially thought. Evidence indicates that Clovis might date as early as 13,400 years ago, which means that it was not the first technology in North America. Given the lack of fluted projectile points in the Old World, it appears certain that the Clovis techno-complex, or at least major components of it, emerged in the New World.

A decorated raven bone from the Zaskalnaya VI (Kolosovskaya) Neanderthal site, Crimea.

We analyze a radius bone fragment of a raven (Corvus corax) from Zaskalnaya VI rock shelter, Crimea. The object bears seven notches and comes from an archaeological level attributed to a Micoquian industry dated to between 38 and 43 cal kyr BP. Our study aims to examine the degree of regularity and intentionality of this set of notches through their technological and morphometric analysis, complemented by comparative experimental work. Microscopic analysis of the notches indicate that they were produced by the to-and-fro movement of a lithic cutting edge and that two notches were added to fill in the gap left between previously cut notches, probably to increase the visual consistency of the pattern. Multivariate analysis of morphometric data recorded on the archaeological notches and sets of notches cut by nine modern experimenters on radii of domestic turkeys shows that the variations recorded on the Zaskalnaya set are comparable to experimental sets made with the aim of producing similar, parallel, equidistant notches. Identification of the Weber Fraction, the constant that accounts for error in human perception, for equidistant notches cut on bone rods and its application to the Zaskalnaya set of notches and thirty-six sets of notches incised on seventeen Upper Palaeolithic bone objects from seven sites indicate that the Zaskalnaya set falls within the range of variation of regularly spaced experimental and Upper Palaeolithic sets of notches. This suggests that even if the production of the notches may have had a utilitarian reason the notches were made with the goal of producing a visually consistent pattern. This object represents the first instance of a bird bone from a Neanderthal site bearing modifications that cannot be explained as the result of butchery activities and for which a symbolic argument can be built on direct rather than circumstantial evidence.

Pathology and mineral composition of bone in the early stages of experimental osteomyelitis

Актуальность Изучение патогенетических закономерностей и характера структурных изменений в костной ткани на ранних стадиях острого остеомиелита является актуальным с точки зрения расширения представлений о механизмах развития осложнений у больных и проведения комплекса реабилитационных мероприятий.Цель работы Комплексная оценка патоморфологических изменений и особенностей минерального состава костной ткани на ранних стадиях развития острого остеомиелита в эксперименте.Материалы и методы Эксперименты проведены на 35 крольчатах обоего пола, в соответствии с биоэтическими требованиями. Острый гематогенный остеомиелит моделировали путем введения культуры стафилококка в костномозговой канал большеберцовой кости. Всех животных выводили из опыта под кратковременным эфирным наркозом путем воздушной эмболии через 30 мин. 6, 12, 24 и 48 часов после введения культуры стафилококка. На данных сроках эксперимента методами световой, электронной (сканирующей и трансмиссионной) микроскопии и рентгеновского электронно-зондового анализа изучались особенности патоморфологии и минерального состава костной ткани.Результаты и их обсуждение Во время первых 30 минут эксперимента микро- и ультрамикроскопические изменения в костной ткани были минимальными: сохранялись костные стержни с небольшими группами эритроцитов, а минеральный состав кости характеризовался снижением объемной доли натрия. На 6 часе эксперимента также не наблюдалось значительных структурных перестроек в костной ткани, однако происходило дальнейшее снижение концентрации натрия и магния, и увеличение объемной доли серы. В то же время, на данном сроке эксперимента отмечено повышение объемных долей кальция и фосфора. Морфологическая картина в последующие 12 часов эксперимента характеризовалась началом деструктивных процессов в костном мозге, с чем, вероятно, связаны некоторые перестройки минерального обмена костной ткани. Наблюдали резкое повышение концентрации натрия, при этом сохранялись высокие значения объемных долей внутрикостного кальция, фосфора и серы, что отражало повышенную минерализацию. К концу 1 суток эксперимента развивались процессы деструкции костной ткани, которые сопровождались деминерализацией. На 2 сутки отмечалась активация процессов остеогенеза, при этом деминерализация сохранялась.Заключение Таким образом, в течение 1-х суток от начала эксперимента структурные перестройки костной ткани отсутствуют, а изменения ее минерального состава связаны с процессами адаптации в ответ на активное воспаление в костномозговом канале. На 2-е сутки опыта развивающаяся деструкция костной ткани сопровождается явлениями остеопороза наряду с активацией остеосинтеза. Период в течение 1-х суток от попадания возбудителя можно рассматривать как наиболее благоприятный с точки зрения профилактики последующих осложнений.

Morphometric and molecular biological features of femoral head tissue in different nosological entities of coxarthrosis

to comparatively analyze the morphometric and molecular biological characteristics of femoral head tissue in different nosological entities of coxarthrosis. A total of 95 samples of femoral head tissue extirpated during hip endoprosthesis in patients with coxarthrosis were investigated. Clinical findings were used to identify the following nosological entities of coxarthrosis: dysplastic, postischemic and posttraumatic. Histological, immunohistochemical and morphometric studies were used. Osteoclast resorptive activity was assessed by determining the cytoplasmic expression of TRAcP (9C5, «Cell Marque»). Vasculogenesis was evaluated by estimating the mean area of vessels with CD34 (QBEnd/10, «Ventana») from the positive stained endothelium and by determining the cytoplasmic expression level of VEGF (SP28, «Spring Bio») in osteoblasts and osteoclasts. Specific histopathological signs were described for each nosological entity of coxarthrosis. Morphometric analysis could reveal a number of additional characteristics of the magnitude of fibrous changes and the thickness of the articular surface and bone rods. Immunohistochemical assessment of molecular biological parameters, such as the expression level of VEGF and TRAcP, also pointed to the characteristic features of bony tissue in the above-mentioned nosological entities of coxarthrosis. In dysplastic coxarthrosis, the maximal expression level of VEGF was recorded in osteoblasts and the expression of VEGF and TRAcP in osteoclasts remained at the minimum level. The lowest expression of VEGF in osteoblasts was found in posttraumatic coxarthrosis. In postischemic coxarthrosis, the highest expression of VEGF and TRAcP was recorded in osteoclasts. The comparative analysis of the morphometric and molecular biological characteristics of femoral head tissue in different nosological entities of coxarthrosis indicated a number of peculiar features. The most specific manifestations of certain morphological and molecular biological signs were identified for each nosological entity of coxarthrosis.

Clovis Beveled Rod Manufacture: An Elephant Bone Experiment

Cylindrical beveled bone rods, upon their initial discovery in 1936 at the Clovis type-site (Blackwater Locality No. 1, NM), were recognized as a component in the subsistence technology of Paleoindian groups. Additional specimens from across North America have since expanded our knowledge of Clovis lifeways. Repeatedly it has been suggested that long bone from either mammoth ( Mammuthus primigenius, M. columbi, M. jeffersonii) or American mastodon ( Mammut americanum) was used in rod manufacture. This notion is logical, given that these artifacts have a dense, massive structure, and remains of both megamammals occur at Clovis sites. Proboscidean limb bone as material for making beveled rods, while likely, remains an untested assumption. This report demonstrates that cortical bone from Elephas maximus (Asian elephant) femur serves well in simulating manufacture of the uni-beveled rod (36-19-6) from Blackwater Draw.

Evidence of Early Paleoindian Bone Modification and Use at the Sheriden Cave Site (33WY252), Wyandot County, Ohio

The analysis of osseous (bone, antler, or ivory) beveled shafts or “rods” has become an important focus in the study of early Paleoindian tool technology. Since 1995 two carved and beveled bone rods have been recovered from Sheriden Cave in northwest Ohio in depositional strata that are radiocarbon dated to between 11,060 and 10,400 radiocarbon years B.P. These strata also contained a small, reworked, Gainey-style fluted point; cut and burned animal bone; and the remains of flat-headed peccary, caribou, giant beaver, and other taxa. The tapered tips and overall morphology of the bone rods demonstrate that they served as projectile points as opposed to other functional types such as foreshafts. Microscopic and radiographic examinations of the bone points reveal that they were manufactured from split sections of mega-mammal bone. These artifacts resemble bone and ivory points found at early Paleoindian sites in western North America and northern Florida but also bear significant morphological similarities to bone sagaie or javelin tips known from Upper Paleolithic sites in Europe. The close spatial and temporal associations between the Sheriden Cave artifacts suggest that they represent the remains of an early Paleoindian tool cache within a small resource extraction campsite.

Prehistoric Osseous Rods from North America: Arguments on Function

Bi-beveled bone rods are sometimes found in association with fluted bifaces. It has previously been proposed that these rods served variously as foreshafts for spears or lances, pressure-flaking tools, butchering tools, sled shoes, composite parts of a longer shaft or rod, and as hafting wedges. The most recent proposal is that they served in pairs as a composite clothes pin foreshaft—a proposal based on their uniquely shaped cross sections and the fact that the angles of the two bevels on each specimen vary. Evaluation of the evidence mustered in support of this proposed model of function indicates the critical data variously 1) do not exist, 2) contradict the model, and 3) are not accounted for by the model.

A Mechanical and Functional Study of Bone Rods from the Richey–Roberts Clovis Cache, Washington, U.S.A.

Bone and ivory (osseous) rods of Paleoindian age have been found over much of North America, several from the same contexts that produced Clovis points. Previous researchers have suggested that these artefacts were projectile points, foreshafts, pressure-flaker handles or sled shoes. Published morphometric data indicate that the rods display varied attribute combinations, but these data are not consistently reported, and no set of typologically definitive attributes has been established. It also is unclear which attributes of the rods are related mechanically to rod function. Experimental replication and mechanical testing of the functional interrelations of numerous attributes of the 14 fluted Clovis points and 14 bone rods recovered from the Richey–Roberts Clovis cache in eastern Washington led to the conclusion that the rods from this site served a primary function as levered hafting wedges used to tighten sinew binding on saw-like implements.