Morphometric characteristics of bone tissue in the treatment of experimental tuberculous osteitis with anti-tuberculosis drugs and selective inhibitors of bisphosphonates

Abstract

Introduction. The effect of bisphosphonates on bone recovery after surgical treatment of osteitis has not been fully understood. Aim: To study morphometric features of bone restoration when bisphosphonates are used after the surgical treatment of experimental tuberculous oste-itis. Material and methods. Experimental model of TB osteitis of distal femur was produced in 21 sexually ma-ture chinchilla male rabbits of distal epimetaphys of fem-oral bone. After resection of the locus and osteoplasty, 3 study arms have been formed depending on the medica-tion therapy: anti-TB drugs only, anti-TB drugs + bisphos-phonates (BF) and BF only. After the end of experiment, 3 and 5 months after surgery, material was harvested in autopsy and histological sections 3–5 microns thick were morphometrically studied on the basis of digital images of the preparations. Results. 3 months after the opera-tion, a greater thickness of the bone rods was noted in the group of rabbits treated with anti-TB drugs + BF, after 6 months — in those treated with BF only (p<0.001). In all the studied groups, relief of specific TB inflammation was achieved in 50–75% of cases 6 months after surgical treatment. Conclusions: The use of BF in the treatment of experimental tuberculous osteitis does not impact the frequency of detection and the area of specific inflamma-tion, while providing the greatest thickness of the bone rods at 3 and 6 months after surgical treatment.