Bone Reduction Research Articles

Pt-Clusters-Equipped Antioxidase-Like Biocatalysts as Efficient ROS Scavengers for Treating Periodontitis.

Developing high-efficiency artificial biocatalysts for scavenging reactive oxygen species (ROS) is critical for treating inflammation diseases and promoting tissue regeneration. By mimicking the active sites in catalase, here, a Pt-clusters-equipped antioxidase-like biocatalysts (Pt─CN) with superior catalytic abilities for stem cell protection and periodontitis treatment are reported. Owing to the excellent effects of multiple Pt clusters, Pt─CN yields exceptional catalytic ROS-scavenging activities for multiple types of ROS. In vitro studies show that Pt─CN can effectively protect stem cell survival, adhesion, and differentiation in a high ROS levels microenvironment. Additionally, Pt─CN can reduce the M1/M2 ratio of macrophages when stimulated by lipopolysaccharide. In vivo treatment of mouse periodontitis further confirms the protection against bone loss and reduction in the inflammatory response. This study provides a basis for the application of biocatalysts with Pt catalytic center in macrophage polarization, stem cell protection, and periodontitis treatment, thus offering a new strategy for the design of high-performance artificial biocatalysts.

Biologic Antiresorptive: Denosumab.

Osteoporosis is an age-related common bone disorder characterized by low bone mineral density and increased fragility fracture risk. Various Antiresorptive medications are being used to target osteoclast mediated bone resorption to prevent bone loss and reduce fracture risk. Denosumab is a novel biological antiresorptive drug that belongs to the class of monoclonal antibodies. It binds to and inhibits the cytokine receptor activator of nuclear factor kappa-B ligand (RANKL), which is requisite for osteoclast differentiation, function and survival. Denosumab has been shown to be a potent and effective therapy for osteoporosis, with clinical trial data demonstrating significant improvement in bone mineral density (BMD) and reductions in fracture risk at various skeletal sites for more than 10 years of treatment. Denosumab has a favourable benefit/risk profile, with low rates of complications such as infection, atypical femoral fracture and osteonecrosis of the jawbone. However, denosumab treatment requires continuous administration, as discontinuation leads to rapid bone mineral loss and increased risk of multiple vertebral fractures due to rebound of bone turnover. Therefore, modification to another anti-osteoporosis drug therapy after denosumab discontinuation is required to maintain bone health. Denosumab is a promising biological antiresorptive therapy for osteoporosis that offers high efficacy and safety, but also poses challenges for long-term management.

A digital technique of bone reduction for a maxillary full-arch implant-supported fixed dental prosthesis.

When fabricating a maxillary full-arch implant-supported fixed dental prosthesis, it is often challenging to systematically reduce alveolar bone to create prosthetic space and hide the prosthesis-tissue junction. This article presents a digital technique that allows for precise bone reduction while simultaneously placing implants and interim prostheses. By using this technique, clinicians can perform surgical procedures in a systematic manner without compromising the functional or esthetic outcomes.

A Method for Precise Reduction of Facial Bone Fractures Using Intraoperative Ultrasound With a Solid Gel Pad as a Coupling Medium.

This study introduces a method to overcome technical challenges in using intraoperative ultrasound for the closed reduction of nasal bone and zygomatic arch fractures. The curvature of the face makes it difficult to apply an ultrasound probe to the facial area. We used a solid gel pad as a coupling medium during surgery to improve the scanning of facial bone fractures. The results show that the fracture sites observed on preoperative computed tomography scans can be easily visualized using intraoperative ultrasound, and real-time manipulation confirms successful reduction. The solid gel pad is light, malleable, easy to use, and provides accurate images. Overall, the use of ultrasound with a solid gel pad enhances the accuracy of closed reduction in facial bone fracture surgeries, confirming fracture patterns and ensuring precise reduction.

Bone repair potential of collagen-poly(3-hydroxybutyrate)-carbon nanotubes scaffold loaded with mesenchymal stem cells for the reconstruction of critical-sized mandibular defects

The poor structural stability of collagen (COL) upon hydration poses a significant challenge in tissue engineering (TE). To overcome this limitation, the incorporation of hydrophobic polymers such as poly(3-hydroxybutyrate) (PHB), and nanomaterials such as carbon nanotubes (CNTs) has been explored. In this study, we investigated the physical, chemical, and biological characteristics of COL-based scaffolds modified with PHB and CNTs for bone tissue engineering (BTE) applications. The tensile strength analysis revealed a substantial improvement in the ultimate tensile strength with the addition of 10 % PHB and 4 % CNTs. Scanning electron microscopy (SEM) images depicted a denser and more compact structure resulting from the presence of PHB and CNTs, enhancing the scaffold's mechanical properties. Fourier-transform infrared spectroscopy (FTIR) confirmed the successful incorporation of PHB and CNTs into the composite scaffold, maintaining the chemical integrity of COL. Stereological studies also conducted in a rat model with induced critical-sized bone defects in the mandibular bone further emphasize the substantial increase in bone formation and reduction in defect volume achieved by the scaffold loaded with stem cells. These findings underscore the promising approach to enhance bone healing, using COL-based scaffolds loaded with stem cells, and the favorable results obtained in this study can contribute to the advancement of BTE strategies.

Association between total cholesterol and lumbar bone density in Chinese: a study of physical examination data from 2018 to 2023

BackgroundThe impact of total cholesterol (TC) on lumbar bone mineral density (BMD) is a topic of interest. However, empirical evidence on this association from demographic surveys conducted in China is lacking. Therefore, this study aimed to examine the relationship between serum TC and lumbar BMD in a sample of 20,544 Chinese adults between the ages of 20 and 80 years over a period of 5 years, from February 2018 to February 2023. Thus, we investigated the effect of serum TC level on lumbar BMD and its relationship with bone reduction in a Chinese adult population.MethodsThis cross-sectional study used data obtained from the Department of Health Management at Henan Provincial People’s Hospital between February 2018 and February 2023. The aim of this study was to examine the correlation between serum TC and lumbar BMD in individuals of different sexes. The research methodology encompassed population description, analysis of stratification, single-factor and multiple-equation regression analyses, smooth curve fitting, and analysis of threshold and saturation effects. The R and EmpowerStats software packages were used for statistical analysis.ResultsAfter adjusting for confounding variables, a multiple linear regression model revealed a significant correlation between TC and lumbar BMD in men. In subgroup analysis, serum TC was found to have a positive association with lumbar BMD in men, specifically those aged 45 years or older, with a body mass index (BMI) ranging from 24 to 28 kg/m2. A U-shaped correlation arose between serum TC and lumbar BMD was detected in women of different ages and BMI, the inflection point was 4.27 mmol/L for women aged ≥ 45 years and 4.35 mmol/L for women with a BMI of ≥ 28 kg/m2.ConclusionIn this study, Chinese adults aged 20–80 years displayed different effects of serum TC on lumbar BMD in sex-specific populations. Therefore, monitoring BMI and serum TC levels in women of different ages could prevent osteoporosis and osteopenia.Trial registrationThe research protocol was approved by the Ethics Committee of Beijing Jishuitan Hospital, in accordance with the Declaration of Helsinki guidelines (No. 2015-12-02). These data are part of the China Health Quantitative CT Big Data Research team, which has been registered at clinicaltrials.gov (code: NCT03699228).

The sacroiliac joint: An original and highly sensitive tool to highlight altered bone phenotype in murine models of skeletal disorders

Bone disorders may affect the skeleton in different ways, some bones being very impaired and others less severely. In translational studies using murine models of human skeletal diseases, the bone phenotype is mainly evaluated at the distal femur or proximal tibia. The sacroiliac joint (SIJ), which connects the spine to the pelvis, is involved in the balanced transfer of mechanical energy from the lumbar spine to the lower extremities. Because of its role in biomechanical stress, the SIJ is a region of particular interest in various bone diseases. Here we aimed to characterize the SIJ in several murine models to develop a highly reliable tool for studying skeletal disorders.We performed a 12-month in vivo micro-computed tomography (micro-CT) follow-up to characterize the SIJ in wild-type (WT) C57BL/J6 mice and compared the bone microarchitecture of the SIJ and the distal femur at 3 months by micro-CT and histology. To test the sensitivity of our methodology, the SIJ and distal femur were evaluated at 3 and 6 months, in 2 murine models of skeletal disorder, X-linked hypophosphatemia (Hyp mice) and HLA-B27 transgenic mice and compared to WT mice. A multimodal analysis was performed, using a combination of microCT and histological analysis.With the Hyp model, the SIJ displayed more bone microarchitecture alterations than the distal femur. Hyp mice showed a significant reduction in trabecular bone at both the distal femur and sacral slope as compared with WT mice, with a significant positive correlation between trabecular bone parameters of the distal femur and sacral side of the SIJ. Furthermore, trabecular bone parameters (Bone Volume/Total Volume (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), trabecular number (Tb.N), trabecular pattern factor (Tb.Pf)) were significantly increased compared to femoral parameters at the SIJ. The sacral articular cortical bone, which is indicative of osteoarticular lesions, was altered in Hyp mice. Interestingly, in accordance to previous studies, HLA-B27 transgenic mice did not show any osteoarticular lesions as compared with WT mice. Cortical bone parameters (thickness, porosity), as well as scoring performed with double blinding, did not show difference between the 2 genotypes.The characterization and evaluation of the SIJ surface appears very sensitive to emphasize alterations of bone and joint. The SIJ may represent a valuable tool to investigate both bone and local osteoarticular alterations in murine models of skeletal disorders and might be a relevant site for assessing the response to treatment of chronic bone diseases.

Comparison of Post Operative Aesthetic Outcome Between Local and General Anesthesia after Close Reduction for Nasal Bone Fracture

Background: Nasal bone fractures are commonest type of facial bone fractures and 3rd most common among all body fractures. Objective: To compare the post operative aesthetic outcome after close reduction of fractured nasal bone when performed under local anesthesia and general anesthesia. Study Design: Randomized control trial. Settings: Department of ENT, Mayo Hospital Lahore Pakistan. Duration: From September 2018 to December 2020. Methods: Seventy patients with diagnosis of nasal bone fracture were included. Patients were randomized into two groups by random number table. Close reduction of Group A was done under local anesthesia and group B under general anesthesia. Pre and post operative aesthetic outcomes were measured by Likert score and nasal angles. Results: Mean age of patients was 23.8 ± 8.59 years. 90% patients were males and 10% were females. Statistically significant difference in aesthetic outcome was present between general and local group at 1st and 3rd month of follow up. Conclusion: In patients of fractured nasal bone post operative aesthetic outcome after close reduction are better under general anesthesia at 1st and 3rd month of follow up. However, at 6th month of follow up results are not statistically significant for reduction under both types of anesthesia.

One-piece Craniofacial Bone Fracture Reduction With an Additional Single Osteotomy at the Zygomaticomaxillary Buttress: A Successful Reduction of Frontoorbitozygomaticomaxillary Fracture Associated With a Temporoparietal Bone Fracture.

A frontoorbitozygomaticomaxillary fracture, also known as a complex midface fracture or "quadripod fracture," is a type of facial fracture involving multiple midface region bones. This includes the frontal bone (forehead), orbitozygomatic complex (which includes the zygomatic bone or cheekbone and the orbital bones around the eye), and the maxillary bone (upper jaw). These complex midface fractures associated with temporoparietal bone fractures can occur in clinical practice. However, a single-piece fracture of these has not been reported before. This study reports a 44-year-old male patient with a significant frontoorbitozygomaticomaxillary fracture associated with a temporoparietal bone fracture combined with epidural hematoma at the superior aspect of unilateral frontoparietotemporal convexity. Surgical treatment was performed 3 weeks after the traumatic event. Despite craniotomy of the parietal bone fragment, the reduction was not possible. The facial bone reduction was finally achieved following an additional single osteotomy at the unilateral zygomaticomaxillary buttress. The follow-up CT scan taken immediately and 3 weeks postoperatively showed successful realignment of craniofacial bone fracture. In conclusion, a single-piece fracture of frontoorbitozygomaticomaxillary bone connected to a temporoparietal bone can require additional osteotomy to achieve successful outcomes in an acute setting.

Flexible Titanium Intramedullary Nail Displacement After Magnetic Resonance Imaging.

A previously healthy 7-year-old boy presented with midshaft radius and ulna malunion after 8 weeks of nonsurgical treatment. He underwent open reduction and internal fixation of both bones with titanium alloy nails and was placed in a long arm cast. Four weeks after surgery, the patient underwent sedated brain MRI and woke up from anesthesia with elbow pain. On cast removal, the ulnar flexible nail was noted to have displaced proximally by 1.5 cm. Despite meeting American Society for Testing and Materials standards, untethered titanium orthopaedic implants are at risk of clinically significant displacement in the early postoperative period.

Phase I study evaluating the Fc-optimized FLT3 antibody FLYSYN in AML patients with measurable residual disease

BackgroundAbout half of AML patients achieving complete remission (CR) display measurable residual disease (MRD) and eventually relapse. FLYSYN is an Fc-optimized antibody for eradication of MRD directed to FLT3/CD135, which is abundantly expressed on AML cells.MethodsThis first-in-human, open-label, single-arm, multicenter trial included AML patients in CR with persisting or increasing MRD and evaluated safety/tolerability, pharmacokinetics and preliminary efficacy of FLYSYN at different dose levels administered intravenously (cohort 1–5: single dose of 0.5 mg/m2, 1.5 mg/m2, 5 mg/m2, 15 mg/m2, 45 mg/m2; cohort 6: 15 mg/m2 on day 1, 15 and 29). Three patients were treated per cohort except for cohorts 4 and 6, which were expanded to nine and ten patients, respectively. Primary objective was safety, and secondary efficacy objective was ≥ 1 log MRD reduction or negativity in bone marrow.ResultsOverall, 31 patients were treated, of whom seven patients (22.6%) experienced a transient decrease in neutrophil count (two grade 3, others ≤ grade 2). No infusion-related reaction or dose-limiting toxicity was observed. Adverse events (AEs) were mostly mild to moderate, with the most frequent AEs being hematologic events and laboratory abnormalities. Response per predefined criteria was documented in 35% of patients, and two patients maintained MRD negativity until end of study. Application of 45 mg/m2 FLYSYN as single or cumulative dose achieved objective responses in 46% of patients, whereas 28% responded at lower doses.ConclusionsFLYSYN monotherapy is safe and well-tolerated in AML patients with MRD. Early efficacy data are promising and warrant further evaluation in an up-coming phase II trial.Trial registration This clinical is registered on clinicaltrials.gov (NCT02789254).

Patient-specific plates for facial fracture surgery: A retrospective case series

ObjectivesSurgeons often encounter challenges when treating maxillofacial fractures using conventional methods that involve trimming or bending ready-made titanium plates for open reduction and internal fixation (ORIF) since it can be time-consuming, imprecise, and inconvenient. This retrospective case series aimed to introduce a novel bone reduction method that utilizes virtual planning, patient-specific surgical guides, and titanium plates. MethodsSeven patients with mandibular symphysis or subcondylar fractures resulting from facial trauma underwent cone-beam computed tomography (CBCT) or facial CT scans, and their medical histories were documented. Virtual surgery was conducted based on three-dimensional (3D) stereolithography images derived from CT scans using the FaceGide software (MegaGen, Daegu, Korea). ORIF was performed using patient-specific surgical guides and plates that were designed, printed, and milled. Radiographic, clinical, and occlusal evaluations were conducted at two weeks and six weeks postoperatively. Subsequently, 3D images from virtual surgery and postoperative CT scans were compared. ResultsThe comparison of 3D virtual surgery and postoperative images revealed minimal surface differences of less than 1 mm. T-scan evaluations indicated that there were no statistically significant differences between the two- and six-week postoperative assessments. Favorable clinical outcomes were observed. ConclusionThis novel method demonstrated stable outcomes in terms of occlusion and healing, with no notable complications. Consequently, this approach may serve as a viable alternative to conventional methods. Clinical SignificanceFacial fracture surgery that utilizes patient-specific surgical guides and plates within a digital workflow can facilitate meticulous surgical planning, reducing the risk of complications and minimizing operation time.

Disease-modifying interactions between chronic kidney disease and osteoarthritis: a new comorbid mouse model

ObjectiveThe prevalence of comorbid chronic kidney disease (CKD) and osteoarthritis (OA) is increasing globally. While sharing common risk factors, the mechanism and consequences of concurrent CKD-OA are unclear. The aims...

Minimally invasive techniques for lateral maxillary sinus floor elevation: small lateral window and one-stage surgery—a 2–5-year retrospective study

This study aimed to introduce a minimally invasive technique for maxillary sinus floor elevation using the lateral approach (lSFE) and to determine the factors that influence the stability of the grafted area in the sinus cavity. Thirty patients (30 implants) treated with lSFE using minimally invasive techniques from 2015 to 2019 were included in the study. Five aspects of the implant (central, mesial, distal, buccal, and palatal bone heights [BHs]) were measured using cone-beam computed tomography (CBCT) before implant surgery, immediately after surgery (T0), 6 months after surgery (T1), and at the last follow-up visit (T2). Patients’ characteristics were collected. A small bone window (height, (4.40 ± 0.74) mm; length, (6.26 ± 1.03) mm) was prepared. No implant failed during the follow-up period (3.67 ± 1.75) years. Three of the 30 implants exhibited perforations. Changes in BH of the five aspects of implants showed strong correlations with each other and BH decreased dramatically before second-stage surgery. Residual bone height (RBH) did not significantly influence BH changes, whereas smoking status and type of bone graft materials were the potentially influential factors. During the approximate three-year observation period, lSFE with a minimally invasive technique demonstrated high implant survival rate and limited bone reduction in grafted area. In conclusion, lSFE using minimally invasive techniques was a viable treatment option. Patients who were nonsmokers and whose sinus cavity was filled with deproteinized bovine bone mineral (DBBM) had significantly limited bone resorption in grafted area.

Partial Extraction Therapy (Part 1): Applications in Full-Arch Dental Implant Therapy.

Partial extraction therapy (PET) is a set of surgical techniques that preserve a portion of the patient's own root structure to maintain blood supply derived from the periodontal ligament complex in order to maintain the periodontium and peri-implant tissues during restorative and implant therapy. PET includes the socket shield technique (SST), proximal shield technique (PrST), pontic shield technique (PtST), and root submergence technique (RST). In a traditional hybrid technique, total extraction and full-arch dental implant therapy often require significant bone reduction and palatal/lingual implant placement. In addition, postextraction preservation of the ridge architecture is a major challenge. This case series demonstrates the use of a combination of PET techniques with digital implant planning and guided implant surgery to achieve highly esthetic outcomes in full-arch implant therapy.

Comparative Study of Clinical and Audiological Outcome between Autologus and Prosthesis in Ossicular Chain Reconstruction

Abstract Introduction: Ossiculoplasty is one of the commonly done and challenging surgery in otology and has been done using various graft materials. Materials such as autologous cartilage (tragal or conchal) or allograft (homograft) of the same tissue and synthetic materials like polyethylene TORP, ceramic and hydroxyapatite PORP or Titanium prosthesis have been used. But the latter synthetic materials are expensive and have high extrusion rates. Autologous cartilage grafts are selected for Ossiculoplasty as they are easy to harvest at the same site of operation, non-toxic, has less extrusion rate, minimum shrinkage and lateralization. Aims and Objective: The aim of this study is to know the efficacy of autologous graft and prosthesis in ossicular reconstruction and to evaluate hearing outcome along with extrusion rate among them. Methodology: It is a prospective study done on patients attending ENT outpatient department in civil hospital Ahmedabad affiliated to BJ medical college Ahmedabad. 60 patients were included in the study who underwent ossicular reconstruction either with canal wall up or down mastoidectomy with tympanoplasty. Conclusion: On follow up of 60 patients over six month we followed there is significant air bone gap reduction with prosthesis but there is risk of extrusion which cancel out beneficial effect of prosthesis. Whereas cartilage and incus remodelling give good result in air bone reduction and better take up compare to prosthesis with less extrusion rate. Also, autologous grafting in ossicular chain reconstruction is economical in developing countries like India with disease prevalent among low socioeconomic group. Keywords: PORP/TORP, Ossicular chain reconstruction, incus remodelling, CSOM, Mastoidectomy

Extraction and Socket Preservation before Implant Placement Using Freeze-Dried Allograft (FDBA) and Platelet-Rich Fibrin in Smokers: Radiographic and Histological Evaluation

Background: Dental extraction can initiate a sequence of biological events that may lead to an insufficient bone volume, which can compromise implant placement. To minimize bone loss, alveolar ridge preservation procedures were developed. However, smoking has been shown to adversely affect the outcomes of the procedures. Hence, this clinical study aimed to assess the ability of platelet-rich fibrin to maintain ridge volume and its ability to generate vital bone in smokers. Methods: Eighteen current heavy smokers with a total of forty upper molars indicated for extraction and implant placement were randomly allocated to four different groups: an advanced platelet-rich fibrin group (A-PRF) (n = 10), a factor-enriched bone graft matrix covered by A-PRF group (A/S-PRF) (n = 10); a freeze-dried bone allograft covered by a crosslinked collagen membrane group (FDBA/CM) (n = 10), which served as a positive control; and a negative-control resorbable collagen plug group (RCP) (n = 10). Two consecutive high-resolution CBCT images were taken for each augmented socket to evaluate the bone volume, one at baseline and the other after six months, and four different measurements (vertical height, horizontal 1 mm H1, horizontal 3 mm, and horizontal 5 mm) were taken for each image. To evaluate the bone vitality, three bone samples were harvested for each group and were analyzed histologically using H and E staining. The results were analyzed using SPSS version 26.0 through the Wilcoxon sign rank test and Kruskal–Wallis test. Results: In terms of preserving bone volume, A/S-PRF showed no significant reduction in bone after six months, while A-PRF showed a significant loss according to two measurements (vertical and horizontal 5 mm), and the last two groups showed a significant loss in all four measurements. For vital bone formation, three groups (A-PRF, A/S-PRF, and RCP) showed the generation of only vital bone, while the fourth group (FDBA/CM) showed the generation of a mixture of vital and non-vital bone. Conclusions: Based on the outcomes of this study, PRF was able to minimize bone loss following dental extraction and generate vital bone in smokers.

Bioactive calcium and mangnesium phosphate bone adhesive for enhanced vascularization and bone regeneration

Bone adhesive is a promising material for the treatment of bone fractures, which is helpful for the fast and effective reduction and fixation of broken bones. However, the existing adhesives bond weakly to bone tissues, and are non-absorbable, or hard to cure under wet conditions. Herein, inspired by the cement-based adhesive used in the industry field, we report a bioactive calcium and magnesium phosphate bone adhesive (MPBA) with the properties of facile preparation, robust adhesion, and bioactive. MPBA is equipped with similar strength to cancellous bones and shows reliable bonding performance for various interfaces, such as Ti6Al4V, Al2O3, and poly(ether-ether-ketone). MPBA achieves excellent bonding ability for the above interfaces with the bonding strengths of 2.28±0.47, 2.32±0.15, and 1.44±0.38 MPa, respectively. Besides, it also shows reliable fixation ability for bovine bone surfaces. The bonding behavior to materials and bones suggests that MPBA could be used for both fracture treatment and implant fixation. Meanwhile, MPBA possesses good biological activity, which could promote the vascularization process and osteogenic differentiation. Finally, in vivo experiments confirmed MPBA can effectively restore bone strength and promote bone regeneration.

Central Giant Cell Granuloma in Pediatric Patient

Central Giant Cell Granuloma (CGCG) is an osteolytic and expansile lesion of the mandible. This lesion affects a younger type of patients which generally require surgical treatment and hospitalization affecting vital structures and causing grow defects. We present a case in a 7-year-old patient using intralesional injections of triamcinolone acetonide. After such a treatment we observed new bone formation and reduction in the size of the lesion which derived in a great outcome in such a young patient. This treatment bypassed any required hospitalization or aggressive surgery reducing postoperative morbidity. This paper report successful treatment of intralesional administration of triamcinolone acetonide in a 7-year-old patient.

A design-of-experiment approach for obtaining Symphytum officinale L. extracts for cosmetic purposes

Promotion of wound or bone healing and reduction of inflammation are commonly known therapeutic qualities of comfrey (Symphytum officinale). Given the presence of active constituents, including allantoin and phenolics, comfrey is widely used in cosmetic and pharmaceutical industries. The aim of this study was to optimize a three-solvent system (water, ethanol, 1,3-propanediol) to maximize the extraction efficiency of allantoin, rosmarinic acid, rabdosiin, and globoidnan A in accordance with the approach of the simplex centroid mixture design. Polynomial regression models of the mixture composition showed the impact of the extracts on cytotoxicity, metalloproteinase inhibition activity, and antioxidant properties response. Additionally, the physical properties, including surface tension and contact angle of the mixture composition, were measured. Based on the maximization of the extraction efficiency of all compounds, an optimum solvent composition was determined as 30.9% of water, 39.2% of ethanol, and 29.9% of propanediol. Such a mixture allowed extraction of 93.6%, 96.8%, 77.0%, and 97.2% of the total amount of allantoin, rosmarinic acid, rabdosiin, and globoidnan A, respectively. The optimal mixture composition for extraction of the compounds was not correlated with inhibition of metalloproteinase activity and cell viability, while a strong positive relationship with antioxidant properties was observed. The optimized solvent composition ensured good extraction efficiency, and the extract had high antioxidant properties. In the near future, in vivo studies with instrumental methods should be conducted to evaluate the practical application of the extracts in the cosmetic industry.