Bone Mineral Content Research Articles

The crucial role of beta-catenin in the osteoprotective effect of semaglutide in an ovariectomized rat model of osteoporosis.

Postmenopausal osteoporosis is a common chronic medical illness resulting from an imbalance between bone resorption and bone formation along with microarchitecture degeneration attributed to estrogen deficiency and often accompanied by other medical conditions such as weight gain, depression, and insomnia. Semaglutide (SEM) is a recently introduced GLP-1 receptor agonist (GLP-1RA) for the treatment of obesity and type 2 diabetes mellitus by mitigating insulin resistance. It has been discovered that the beneficial effects of GLP-1 are associated with alterations in lipolysis, adipogenesis, and anti-inflammatory processes. GLP-1 analogs transmit signals directly to adipose tissue. Mesenchymal stem cells (MSCs) are multidisciplinary cells that originate from bone marrow, migrate to injury sites, and promote bone regeneration. MSCs can differentiate into osteoblasts, adipose cells, and cartilage cells. Our aim is to investigate the role of semaglutide on bone formation and the Wnt signaling pathway. Osteoporosis was induced in female rats by ovariectomy, and the ovariectomized rats were treated with alendronate as standard treatment with a dose of 3mg/kg orally and semaglutide with two doses (150 mcg/kg and 300 mcg/kg) S.C. for 10 successive weeks. Semaglutide ameliorates bone detrimental changes induced by ovariectomy. It improves bone microarchitecture and preserves bone mineral content. Semaglutide ameliorates ovariectomy-induced osteoporosis and increases the expression of β-catenin, leading to increased bone formation and halted receptor activator of nuclear factor kappa-Β ligand (RANKL's) activation. Semaglutide can be used as a potential prophylactic and therapeutic drug against osteoporosis, possibly by activating Wnt signaling and decreasing bone resorption.

Assessing the Muscle-Bone Unit in Girls Exposed to Different Amounts of Impact-Loading Physical Activity-A Cross-Sectional Association Study.

In children, an association exists between muscle and bone, as well as between physical activity and osteogenesis. Impact loading is a factor in increasing bone accrual during growth. In this work, we explored the muscle-bone association in girls exposed to long-term physical activity at different levels of impact loading. Four groups of girls aged 7-16 were considered. The curricular (C; n = 22) group only had curricular physical activity at school (2 h/w). In addition to curricular physical activity, the girls in the dance (D; n = 21), gymnastics at lower training (GL; n = 14), and gymnastics at higher training (GH; n = 20) groups had 2 h/w, 4 h/w, and 4 h/w < training ≤ 12 h/w additional physical activity, respectively, for at least one year. A visual analysis estimated the respective amounts of impact-loading activity. The bone mineral content (BMC), areal bone mineral density (aBMD), and fat-free soft tissue mass (FFSTM) were assessed with dual-energy X-ray absorptiometry. The results showed that, after adjusting for several confounders, statistically significant correlations were present between muscle mass and several bone mineral variables. A regression analysis confirmed the correlation in the data, and showed the marginal role of other body composition variables and physical activity for predicting BMC and BMD. Skeletal muscle mass is a major determinant of the BMC and BMD of the TBLH, as well as of the Appendicular level, in girls exposed to different amounts of long-term impact-loading physical activity.

Potential Effects of Lifelong Team Handball and Football Training and Nutritional Habits on Bone Health and Body Composition in Elderly Women.

The aim of this study was to evaluate the effects of lifelong team handball/football training on regional bone health and body composition in elderly women. Seventeen elderly women team handball/football players (65.9 ± 5.7 years) and twenty-one untrained age-matched women (controls) (67.7 ± 5.1 years) participated. Whole-body and regional dual-energy X-ray absorptiometry scans of arms, legs, and lower spine (L1-L4) were performed. We observed 8% and 9% higher bone mineral density (BMD) and bone mineral content (BMC), respectively, at the whole-body level and in the legs and 11.5% higher BMC in the legs in team handball/football players compared to untrained age-matched controls (p < 0.05). Higher total and leg lean body mass (p < 0.05), along with lower total body fat percentage (p < 0.05) and higher T- and Z-scores, markers of fragility risk fracture (0.294 ± 1.461 vs. -0.538 ± 1.031; 1.447 ± 1.278 vs. 0.724 ± 0.823, respectively), were also found in team handball/football players compared to controls (p < 0.05). No significant differences in nutritional habits were observed between groups. Our study suggest that the beneficial effects of lifetime handball/football practice on bone preservation in elderly women occur independently from nutritional intake, which emphasize the potential role of team sports in osteoporosis prevention. Future studies should focus on the cofounding factors and causative mechanisms mediated by team sport practice in osteoporosis prevention.

Preliminary study on the effect of lumbar axial rotation on bone mineral density measured by DXA and QCT

Currently, the relationship between axial rotation of the vertebrae and bone mineral density (BMD) measured by dual-energy X-ray absorptiometry (DXA) and quantitative computed tomography (QCT) remains controversial. The aim of this study is to quantitatively assess the effect of vertebral rotation on volumetric bone mineral density (v-BMD) and areal bone mineral density (a-BMD), further to propose the corrected strategies. To achieve this, a phantom, which was rotated from 0° to 25° in 5° increments, was utilized. Bone mineral content (BMC), a-BMD, v-BMD, and projected area (p-AREA) were measured. The Kruskal–Wallis non-parametric test or one-way ANOVA was used to examine the differences in variables between the different groups. The Pearson and Spearman correlation was used to test the relationships between quantitative parameters and rotated angles. Linear regression analysis was used to evaluate the relationship between angles and quantitative parameters. The findings indicate that, as the angle increased, a-BMD and v-BMD decreased (P < 0.001) , and the p-AREA increased (P < 0.001), but the BMC stays constant. The rotated angle was negative correlated (r = − 0.925, P < 0.001) with a-BMD and v-BMD (r = − 0.880, P < 0.001), positive (r = 0.930, P = < 0.001) correlated with p-AREA. The linear regression analysis showed that a-BMD = 0.808–0.01 × Angle and v-BMD = 151.808–1.588 × Angle. This study showed that, axial rotation might lead to a lower measured for a-BMD and v-BMD, it should be modified. This gives clinicians some insights into how to deal with osteoporosis in scoliosis patients. It's essential for clinicians to incorporate these findings into their diagnostic processes to prevent potential misdiagnosis and over-treatment of osteoporosis.

Effects of recreational artistic gymnastics on bone mineral density and content of school-age girls

Aims :This study aimed to evaluate whether Artistic Gymnastics (AG) practice has beneficial effects on Bone Mineral Density (BMD) and Bone Mineral Content (BMC) of young gymnasts.Methods : All girls involved in AG training from the city of Pelotas, aged 6 to 12 years were recruited. Control group participants could not be involved in any extracurricular sports activity. Predictive equations based on anthropometric and physical tests were used to assess bone mass (BMD and BMC).Results : When compared to control group, AG group showed higher Femoral neck (0.672 ± 0.147 vs 0.561 ± 0.172) and Upper limbs (1.482 ± 0.159 vs 1.289 ± 0.178) BMD. In addition, Lumbar spine BMD was higher in AG group regardless of the predictive equation used. When compared with girls not involved in extracurricular activities, AG practitioners presented higher upper limbs, femoral neck and lumbar spine BMD.Conclusion : However, caution is required when using predictive equations to assess BMD and BMC.

The Impact of Blood Flow Restriction Training on Tibial Bone Stress Injury Rehabilitation: An Exploratory Case Series.

Lower extremity bone stress injuries (BSI) are common injuries among athletes and military members. Typical management involves a period of restricted weightbearing which can have rapid detrimental effects upon both muscle and bone physiology. Few studies have investigated the effect of blood flow restriction (BFR) training on bone in the rehabilitative setting. The purpose of this study was to investigate the effects of lower extremity exercise with the addition of BFR upon bone mineral density, bone mineral content, and lean body mass in military members with tibial BSIs. Case series. Twenty military members with MRI-confirmed tibial BSI were recruited to complete lower extremity exercise with the addition of BFR twice per week for four weeks. The BFR cuff was applied proximally to the participant's involved limb while they performed gluteal, thigh, and leg resistance exercises. Outcomes were assessed at baseline and four weeks. The primary outcomes were whole leg bone mineral density (BMD), bone mineral content (BMC), and lean body mass (LM) as measured by dual-energy x-ray absorptiometry. Secondary outcomes included thigh and leg circumference measures and patient-reported outcomes, including the Lower Extremity Functional Scale (LEFS), Patient-Reported Outcomes Measurement Information System 57 (PROMIS-57), and Global Rating of Change (GROC). No significant differences were found in BMD (p=0.720) or BMC (p=0.749) between limbs or within limbs over time. LM was generally less in the involved limb (p=0.019), however there were no significant differences between or within limbs over time (p=0.404). For thigh circumference, significant main effects were found for time (p=0.012) and limb (p=0.015), however there was no significant interaction effect (p=0.510). No significant differences were found for leg circumference (p=0.738). Participants showed significant mean changes in LEFS (15.15 points), PROMIS physical function (8.98 points), PROMIS social participation (7.60 points), PROMIS anxiety (3.26 points), and PROMIS pain interference (8.39 points) at four weeks. The utilization of BFR in the early rehabilitative management of tibial BSI may help mitigate decrements in both bone and muscle tissue during periods of decreased physical loading. 4.

Pregnancy vitamin D supplementation and offspring bone mineral density in childhood follow-up of a randomized controlled trial

BackgroundFindings from the MAVIDOS trial demonstrated a positive effect of gestational cholecalciferol supplementation on offspring bone mineral density (BMD) at age 4 y. Demonstrating the persistence of this effect is important to understanding whether maternal vitamin D supplementation could be a useful public health strategy to improving bone health. ObjectivesWe investigated whether gestational vitamin D supplementation increases offspring BMD at ages 6–7 y in an exploratory post-hoc analysis of an existing trial. MethodsIn the MAVIDOS randomized controlled trial, pregnant females <14 wk’ gestation with a singleton pregnancy and serum 25-hydroxyvitamin D 25–100nmol/l at 3 United Kingdom hospitals (Southampton, Sheffield, and Oxford) were randomly assigned to either 1000 IU/d cholecalciferol or placebo from 14 to 17-wk gestation until delivery. Offspring born at term to participants recruited in Southampton were invited to the childhood follow-up at ages 4 and 6–7 y. The children had a dual-energy X-ray absorptiometry (DXA, Hologic discovery) scan of whole-body-less-head (WBLH) and lumbar spine, from which bone area, bone mineral content (BMC), BMD, and bone mineral apparent density (BMAD) were derived. Linear regression was used to compare the 2 groups adjusting for age, sex, height, weight, duration of consumption of human milk, and vitamin D use at 6–7 y. ResultsA total of 454 children were followed up at ages 6–7 y, of whom 447 had a usable DXA scan. Gestational cholecalciferol supplementation resulted in higher WBLH BMC [0.15 SD, 95% confidence interval (CI): 0.04, 0.26], BMD (0.18 SD, 95% CI: 0.06, 0.31), BMAD (0.18 SD, 95% CI: 0.04, 0.32), and lean mass (0.09 SD, 95% CI: 0.00, 0.17) compared with placebo. The effect of pregnancy cholecalciferol on bone outcomes was similar at ages 4 and 6–7 y. ConclusionsSupplementation with cholecalciferol 1000 IU/d during pregnancy resulted in greater offspring BMD and lean mass in mid-childhood compared with placebo in this exploratory post-hoc analysis. These findings suggest that pregnancy vitamin D supplementation may be an important population health strategy to improve bone health. Trial registration numberThis trial was registered at the ISRCTN (https://doi.org/10.1186/ISRCTN82927713) as 82927713 and EUDRACT (https://www.clinicaltrialsregister.eu/ctr-search/trial/2007-001716-23/results) as 2007-001716-23.

Physiological fitness in people with advanced multiple sclerosis

IntroductionPhysiological fitness, encompassing cardiorespiratory fitness (CRF) and body composition, are important markers of overall health, functional capacity, and quality of life in general and clinical populations. Characterizing fitness is important for the development of tailored interventions and strategies to optimize well-being across the spectrum of disability in multiple sclerosis (MS). While existing research has explored fitness in people with mild-to-moderate disability, there is a scarcity of data in people with advanced MS (Expanded Disability Severity Scale, EDSS≥7.0). ObjectiveTo characterize CRF and body composition and their associations with fatigue, quality of life, and function in individuals with advanced MS. MethodsParticipants (n=18, mean age=60.7 years-old, median EDSS=7.5) underwent a cardiopulmonary exercise test and dual-energy X-ray absorptiometry scanning. Main outcomes included peak volume of oxygen uptake (V̇O2peak) and whole and regional body fat, lean mass, bone mineral content, and bone density. Symptoms of fatigue (Modified Fatigue Impact Scale, MFIS), quality of life (29-item MS Impact Scale, MSIS-29), and daily function (Late-Life Function and Disability Instrument, LLFDI) were collected. ResultsParticipants exhibited notably low CRF levels (V̇O2peak=9.8 mL/min/kg) and poor body composition (lower lean mass, bone mineral content and density) compared to previous studies in the general population and in individuals with MS with lower disability. V̇O2peak was most consistently associated with function in daily life (LLFDI scores, rs≥0.637, p≤0.004). ConclusionThese findings reinforce the potential importance of physiological fitness to preserve function in people with advanced MS.

Do cardiometabolic risk factors mediate the relationship between body composition and bone mineral content in South Indian children aged 5 to 16 years?

The complex interplay between adiposity, bone health and cardiometabolic risk (CMR) factors is unclear in Indian children. We aimed to investigate the mediating role of number of CMR factors on the relationship between fat % and bone mineral content (BMC) % in South Indian children aged 5-16 years. Healthy children (n = 317), from India, underwent anthropometric, blood biochemistry, blood pressure, along with body composition and BMC assessments using Dual-energy X-ray absorptiometry. Based on the number of CMR factors, children were categorised into three groups: 0, 1 and ≥ 2. Analysis of variance was used to compare the parameters between the CMR groups and mediation analysis was performed to examine if the number of CMR factors mediated the relationship between fat % and BMC %. The prevalence of 0, 1 and ≥ 2 CMR factors was 42.3%, 33.9% and 23.9% respectively; mean BMC % was lowest in ≥ 2 CMR group. In the whole group, BMC % had significant negative correlation with fat % (r = -0.68, p < 0.0001) and positive correlation with lean % (r = 0.64, p < 0.0001). Adjusted for age and sex, results suggested significant mediating effect of number of CMR factors on the relationship between fat % and BMC % (Average Causal Mediation Effects =-0.002, bootstrapped 95% CI: -0.0039, -0.0001, p < 0.01), but losing significance when adjusted for co-variates. Number of CMR factors mediates the relationship between fat % and BMC % in Indian children. Further studies are needed to confirm these findings, understand mechanisms and plan appropriate strategies.

Comparing body composition measures in children with end stage liver disease using noninvasive bioimpedance analysis

BackgroundChronic liver disease (CLD) in children, often leads to cirrhosis and end-stage liver disease (ESLD). CLD poses significant challenges in management and prognosis. Assessing body composition, including sarcopenia, is increasingly recognized as important in understanding outcomes in this population.MethodsWe conducted a prospective observational study, involving children aged 2 to 18 years with ESLD awaiting liver transplantation. Socio-demographic, clinical, and laboratory data were collected, and body composition was assessed using Bioelectrical Impedance Analysis (BIA). Sarcopenia was defined using age-specific cut-off points for appendicular skeletal muscle mass (aSMM) and fat-free mass (FFM).ResultsThe study included 57 children (42.1% girls, 57.9% boys; median age: 10.9 years) with liver cirrhosis. Of them 11 (19.3%) died during the study. The mean duration of living with end-stage liver disease prior to participation was 5.43 years [IQR: 3.32, 8.39]. The most common etiology was biliary atresia (24.6%), followed by cryptogenic (22.8%). Deceased children exhibited significantly higher sarcopenia prevalence, lower basal metabolic rate and growth scores compared to survivors (P < 0.05), (771.0 vs. 934.0, P = 0.166) (65.0 vs. 80.5, P = 0.005). Total body and limb-specified lean mass were lower in deceased children, although not statistically significant. Similarly, total mineral (90% normal) and bone mineral content were lower in deceased children, with a significant difference observed only in water-to-FFM percentage (72.5 vs. 73.1, P = 0.009).ConclusionThis study highlights the high prevalence of sarcopenia among children with ESLD and its association with adverse outcomes, including mortality. Bioimpedance analysis emerges as a promising, non-invasive method for assessing body composition in pediatric ESLD, warranting further investigation and integration into clinical practice.

Nutritional Impacts of Dietary Selenium, Iodine and their Interaction on Egg Performance, and Antioxidant Profile in Laying Longyuan Duck Breeders.

The present study aimed to optimize the combined effect of dietary selenium (SE) and iodine (ID) on the productive and reproductive performance and antioxidant capacity of Longyuan breeding ducks. A total of 288 Longyan duck breeders aged 20 wk were randomly assigned to four groups with six replicates (n = 72 ducks/group; 12 ducks/replicate). A 2 × 2 factorial arrangement experiment was performed and included 2 supplementation levels of each SE and ID for 200 days of the experimental period. The first group (SE0/ID0) received a basal diet without SE or ID supplementation and was considered to be the control group, whereas the other three groups, SE0/ID4, SE2/ID0 and SE2/ID4, received a basal diet supplemented with 0.4 mg ID/kg, 0.2 mg SE/kg or 0.2 mg SE supplemented with 0.4 mg ID/kg, respectively. The results indicated that the albumin height of the SE2/ID0 group was lower (P < 0.05) than that of the control group, that the egg shape index of the SE2/ID4 and SE0/ID4 groups were lower (P < 0.05) than that of the control group (SE0/ID0), and that the SE concentration significantly increased (P < 0.05) in the SE2/ID0 and SE2/ID4 groups. Hatchability and embryonic mortality improved (P < 0.05) in the SE2/ID0 group. Plasma GSH-Px activity was increased (P < 0.05) by reducing the concentration of malondialdehyde (MDA) in the SE groups. In addition, the tibia length significantly increased (P < 0.05) in the ID (SE0/ID4 and SE2/ID4) groups compared with that in the control group, the plasma content of IGF-1 in the SE2/ID4 and SE0/ID4 groups were greater (P < 0.05) than that in the control group, and the bone mineral content increased (P > 0.05) in the SE2/ID0 and SE0/ID0 groups. Compared with those in the other groups, the mRNA expression of antioxidant-related genes, including Nrf2 and SHMT1 in the SE2/ID4 group was upregulated (P > 0.05), especially in the SE2/ID4 group. Overall, dietary treatment with SE2/ID4 (0.2 mg SE in 0.4 mg ID/kg diet) could be a suitable feed supplement for improving the the egg quality, health status, endogenous antioxidant content, antioxidant-related gene expression and pre-hatching quality of Longyuan duck breeders.

Adam19 Deficiency Impacts Pulmonary Function: Human GWAS Follow-up in a Mouse Knockout Model

PurposeOver 550 loci have been associated with human pulmonary function in genome-wide association studies (GWAS); however, the causal role of most remains uncertain. Single nucleotide polymorphisms in a disintegrin and metalloprotease domain 19 (ADAM19) are consistently related to pulmonary function in GWAS. Thus, we used a mouse model to investigate the causal link between Adam19 and pulmonary function.MethodsWe created an Adam19 knockout (KO) mouse model and validated the gene targeting using RNA-Seq and RT-qPCR. Mouse body composition was assessed using dual-energy X-ray absorptiometry. Mouse lung function was measured using flexiVent.ResultsContrary to prior publications, the KO was not neonatal lethal. KO mice had lower body weight and shorter tibial length than wild-type (WT) mice. Their body composition revealed lower soft weight, fat weight, and bone mineral content. Adam19 KO had decreased baseline respiratory system elastance, minute work of breathing, tissue damping, tissue elastance, and forced expiratory flow at 50% forced vital capacity but higher FEV0.1 and FVC. Adam19 KO had attenuated tissue damping and tissue elastance in response to methacholine following LPS exposure. Adam19 KO also exhibited attenuated neutrophil extravasation into the airway after LPS administration compared to WT. RNA-Seq analysis of KO and WT lungs identified several differentially expressed genes (Cd300lg, Kpna2, and Pttg1) implicated in lung biology and pathogenesis. Gene set enrichment analysis identified negative enrichment for TNF pathways.ConclusionOur murine findings support a causal role of ADAM19, implicated in human GWAS, in regulating pulmonary function.

Structural Equation Modeling (SEM) of the interaction between cholecalciferol treatment and external factors in the incidence of vertebrae malformations in the hybrid grouper Epinephelus fuscoguttatus X Epinephelus polypehadion

Vitamin D3(VD3), also known as cholecalciferol plays a crucial role in maintaining calcium and phosphate homeostasis and has the potential to suppress bone malformations in fish larvae. However, external factors during growth also significantly influence the development of fish larval bones. Understanding the complex interaction between VD3 treatment and these external factors is crucial for optimizing therapeutic potential and effectively treating bone anomalies. SEM (Structural Equation Modeling) offers a comprehensive framework to explore these interactions by considering multiple variables simultaneously and examining their interrelationships. This study aimed to determine the impact of VD3 and its relationship with other external factors on the incidence of bone malformations in hybrid grouper larvae (Epinephelus fuscoguttatus X Epinephelus polyphekadion, EFEP). The treatments included a dose of 1.9 IU/g of VD3 in the feed and control group without VD3. All treatments received standard enrichment ingredients including vitamin C, taurine, and commercial enrichment (*SELCO). The enrichment was applied to rotifers (3 – 25 days after hatching, DAH) and artificial feed (25 – 35 DAH). The treatment was conducted in two batches, during the dry season (batch 1) and the rainy season (batch 2), to explore the seasonal impact on bone malformations. Results indicated that VD3 reduced the incidence of bone malformations in EFEP hybrid grouper fry by 1.14–1.65 times. SEM analysis revealed that spawning timing, egg quality, and water quality significantly impacted the occurrence of bone deformities, influencing malformations directly and indirectly through bone mineral content. This study demonstrated that bone malformations can be result from deficiencies in calcium, excess potassium and sulfur, and the presence of bromine in bone.

Effects of Acid Pre-Treatment on Removal of Organic and Inorganic Substances from Black Tilapia Bones and Scales

Fish bones and scales are good sources of gelatin. However, high mineral content in bones and scales often lead to low quality gelatin with high percentage of ash. Therefore, pre-treatment with acid prior to gelatin extraction is necessary. Black tilapia (Oreochromis niloticus) bones and scales were subjected to Hydrochloric (HCl) acid of 0.6 M to 1.4 M for 0 to 32 hr. The suitable pre-treatment conditions were determined using percentage of ash removed and loss of protein from the samples. Results obtained indicate, 99.86 % and 99.84 % of ash can be removed from bones and scales, with 1.4 M HCl (32 hr) and 1.0 M HCl (10 hr), respectively. Further analysis with UV-Vis revealed that, excessive protein breakdown starts to occur at 1.0 M (32 hr) and 0.6 M (12 hr), in bones and scales, respectively. The data suggest using pre-treatment conditions lower than the above to avoid protein loss which may lead to low gelatin yield.

Testing the hypothesis of a nested block covariance matrix structure with applications to medicine and natural sciences

This paper addresses the challenge of testing the hypothesis of what the authors call a nested block circular‐compound symmetric (NBCCS) covariance structure for the population covariance matrix. This is a covariance structure which has an outer block compound symmetric structure, where the diagonal blocks are themselves block circular matrices, while the off‐diagonal blocks are formed by all equal matrices. The NBCCS null hypothesis is decomposed into sub‐hypotheses, allowing this way for a simpler way to obtain a likelihood ratio test and its associated statistic. The exact moments of this statistic are derived, and its distribution is carefully examined. Given the complicated nature of this distribution, highly precise near‐exact distributions were developed. Numerical studies are conducted to assess the proximity between these near‐exact distributions and the exact distribution, highlighting the performance of these approximations, even in the case of very small sample sizes. Furthermore, three datasets, on bone mineral content, metabolic rates of glucose, and soil moisture are used to exemplify the practical application of the methodology derived in this study.

Effect of dietary daidzein supplementation on reproductive performance, egg quality and bone mineralization in laying hens at first-cycle of production via reproductive-growth axis

Daidzein (Da), an isoflavone, resembles natural oestrogen in structure and activity. We investigated the effects of dietary Da on the reproductive performance, egg quality, and bone mineralisation of hens in the pre-peak laying period. A total of 432 laying hens (110 days) were randomly allocated to control (CON) or Da-supplemented diet (10, 25, and 50 mg/kg). Each treatment had six replicates of 18 birds/replicate, and the feeding trial lasted 8 weeks. Supplementation of dietary Da significantly improved laying rate, egg weight, egg mass, and feed-to-egg ratio (p < 0.05). Likewise, dietary Da significantly improved albumen quality (Haugh unit, thick albumen fraction, and albumen proportion) and eggshell quality (eggshell; strength, eggshell thickness, gloss, and colour) (p < 0.05). Dietary Da exerted a notable significant increase (p < 0.05) on magnum weight, follicle numbers, serum levels of growth hormones, thyroid hormones (T3 and T4), reproductive hormone (oestrogen, follicle-stimulating hormone, luteinizing hormone), and mRNA expression of related genes (ESR1, FSHR1, PRL1, GNHR1). Also, Da significantly increased serum levels of superoxide dismutase, catalase, glutathione and decreased malondialdehyde (p < 0.05). Furthermore, the Da groups showed enhanced tibia strength, length, phosphorus, and bone mineral content (p < 0.05). In summary, dietary Da enhanced early onset of lay and improved albumen, eggshell, and bone quality in laying hens during the pre-peak period. Positive outcomes may be due to the efficient metabolism of Da and the safety level of inclusion. The enhancement of reproductive hormones, related genes, and antioxidant enzymes may explain daidzein’s effects on laying hens. Highlights Daidzein improved egg production significantly. Albumen, eggshell and tibia quality were enhanced. Optimal inclusion level at 25 or 50 mg/kg was safe.

Effects of dietary free fatty acid content and degree of fat saturation on tibia bone properties of laying hens

Acid oils and fatty acid distillates are fat by-products of the refining process of edible oils and are characterized by their high proportion of free fatty acids (FFA). While lipids are essential in poultry diets, their chemical structure may interfere with calcium absorption. Therefore, this study investigated the effects of dietary FFA content and the degree of fat saturation on bone metabolism in commercial layers. For 15-wk, a total of 144 laying hens (19-wk-old) were randomly assigned to 8 treatments (6 replicates with 3 birds each), which were obtained by gradually replacing crude soybean oil (rich in unsaturated fatty acids [UFA]) with soybean acid oil (rich in UFA and FFA), or crude palm oil (rich in saturated fatty acids [SFA]) with palm fatty acid distillate (rich in SFA and FFA). Following a 2 × 4 factorial design, 4 UFA-rich and 4 SFA-rich diets were created with varying FFA content: 10, 20, 30, and 45%. Tibiae (6 birds/treatment) were collected at the end of the trial for the assessment of mineral composition, morphological properties, and mechanical characteristics. The data were analyzed using a 2-way ANOVA with the GLM procedure. Orthogonal polynomial contrasts were employed to determine the linear effect of increasing %FFA, with statistical significance set at P < 0.05. The degree of saturation was found to negatively impact on calcium and phosphorus bone content, with higher levels found in soybean-based diets (P < 0.001). A significant interaction was observed for medullary bone mineral content, showing a linear decrease as the dietary %FFA increased (P < 0.05) in palm diets. In contrast, morphological and mechanical bone traits, total ash content, and cortical bone mineral composition remained unaffected (P > 0.05). These results suggest that the degree of fat saturation exerts a greater impact than FFA content on bone mineral metabolism, supporting the commercial use of fat by-products rich in FFA in laying hen diets, at least during the early stages of the laying cycle.

Relationship between Body Mass Index and Health-Related Physical Fitness Components in HIV-Diagnosed Children and Adolescents.

There is a need to monitor physical fitness in HIV-diagnosed children and adolescents, and body mass index (BMI) could be an option for this due to its usability for assessing nutritional status and fat mass. The present study aimed to explore the relationship between BMI and physical fitness in HIV-diagnosed children and adolescents. A cross-sectional study was conducted with 86 HIV-diagnosed children and adolescents aged 5-15, with participants from two research protocols (Study I, n = 65; Study II, n = 21). Physical fitness was assessed through body composition (anthropometric measurements and dual energy X-ray absorptiometry), cardiorespiratory fitness (peak oxygen consumption [VO2peak]), muscle strength/endurance (handgrip strength, standing broad jump, and abdominal and modified push-up endurance), and flexibility (sit-to reach test). The relationship between BMI and physical fitness components was analyzed through correlation and simple and multiple linear regression analysis. Eutrophic participants (mean age 11.44 ± 2.20) presented a normal fat mass percentage and overweight participants (mean age 11.50 ± 2.54) presented adequate handgrip strength. The adjusted models could explain 71% of fat-free mass, 57% of fat mass percentage, 70% of bone mineral content, 72% of bone mineral density, and 52% of handgrip strength. Increases in BMI were associated with increases in fat-free mass, fat mass percentage, bone mineral content, bone mineral density, and handgrip strength. BMI was capable of distinguishing those presenting a normal fat mass percentage and those presenting adequate handgrip strength.

Genome-wide association study for polledness, horn shape, and wool traits in Original Valachian sheep

Abstract. The Original Valachian sheep is an endangered Slovak national breed that is well adapted to high-altitude pastures. The sheep can be horned with various shapes and can have multi-coloured or completely white or black wool. Breeders are interested in learning about the genetic basis of these traits. We conducted a genome-wide association study based on the genomic information of 96 sheep genotyped by the GeneSeek GGP Ovine 50K SNP (single-nucleotide polymorphism) chip and on the following traits: polledness (presence or absence of horns), horn shape, and wool colour (completely white and completely black). The univariate linear mixed model was used to discover genetic variants significantly associated with tested traits. The Bonferroni correction and the false-discovery rate were used as significance thresholds. The RXFP2 gene (chromosome 10, 29.5 Mb) was identified as a strong candidate for polledness. In addition, when compared to animals with sideways-turned horns vs. polled, the region around the ADAMTS3 gene (chromosome 6, 88.47 Mb) was significant. A total of nine significant genomic regions were found when comparing the sideways-turned spiral horns with the backwards-curled horns, the two most frequent horn types in Original Valachian sheep. The RXFP2 may also contribute to the genetic control of horn shape. Genes identified in other regions were involved to osteogenic differentiation and osteoblast proliferation (PCP4, chromosome 1, 260.7 Mb), bone mineral density and mineral content (NKX1-2, chromosome 22, 43.75 Mb). The significant genetic variants close to the region of MC1R (chromosome 14, at 14.2 Mb) were associated with the wool colour of sheep that were fully white or fully black animals. The results of this study will contribute to a better understanding of the phenotypic variability of the Original Valachian sheep, especially regarding traits that are very important for breeders of this endangered breed.

Bodyweight influences the relationship between serum testosterone and bone mineral density in men with spinal cord injury.

Cross-sectional study. To examine the association between serum testosterone levels (T levels) and bone mineral density after spinal cord injury (SCI). Medical research center. Body composition assessments were measured in 53 men with chronic SCI. Serum T levels were measured after an overnight fast. Total and regional bone mineral density (BMD) and bone mineral content (BMC) were measured using dual-energy X-ray absorptiometry. Participants were classified into three groups based on their body weight [<65 kg, 65-80 kg, >80 kg] or serum T levels into low (400 ng/dl), mid-normal (401-544 ng/dl) and normal (>545 ng/dl) ranges. Serum T level was negatively related to body weight (r = -0.33, P = 0.016), fat mass (r = -0.46, P < 0.001) and percentage fat mass (r = -0.48, P < 0.001). There were no significant relationships between serum T levels and any of the bone health measurements. Body weight was related to total, regional (P < 0.01 for both) and knee BMD (P < 0.05). T level was only related to total and regional BMD in the group with body weight of 65-80 kg. Testosterone has no direct relationship with BMD except within a specific weight group. However, body weight or fat mass negatively influences circulating T levels in men with SCI. The relationship between serum T levels and BMD is mediated by body weight in men with SCI.