Bone Marrow Polychromatic Erythrocytes Research Articles

Methylated and ethylated dialkylphosphate metabolites of organophosphate pesticides: DNA damage in bone marrow cells of Balb/c mice.

Dialkylphosphates (DAPs), metabolites of organophosphate (OP) pesticides, are widely distributed in the environment and are often used as biomarkers of OP exposure. Recent reports indicate that DAPs may be genotoxic, both in vitro and in vivo. We have examined the genotoxicity of the methylated DAPs dimethyldithiophosphate (DMDTP) and dimethylphosphate (DMTP) and the ethylated DAPs diethyldithiophosphate (DEDTP) and diethylphosphate (DETP), in comparison with their parental compounds, malathion and terbufos, respectively, in bone marrow polychromatic erythrocytes (PCE) of male and female Balb/c mice. We also compared DNA damage (comet assay) induced by DMDTP and dimethyl phosphate (DMP) in human cell lines. Both DMDTP and DMP caused DNA damage in peripheral blood mononuclear cells, HeLa cells, and the hepatic cell lines HepG2 and WRL-68. In the in vivo micronucleus assay, methylated and ethylated DAPs increased micronucleated PCE cells in both male and female mice. Female mice were more susceptible to DNA damage. In comparison to their parental compounds, methylated DAPs, particularly DMTP, were more genotoxic than malathion; DEDTP, DETP, and terbufos were similar in potency. These results suggest that DAPs may contribute to DNA damage associated with OP pesticide exposure.

Genotoxic and cytotoxic potential of food azodyes: preclinical safety assessment using the in vivo micronucleus assay

This study evaluated the genotoxic effects of azodyes (ponceau 4R, red 40, sunset yellow and tartrazine) by the use of in vivo micronucleus assays. Swiss albinus young adult mice of both sexes, healthy and heterogeneous, were used in the micronucleus assay. Groups of animals were treated using a single dosing regimen and euthanized at 24 and 48 hours. The study design included treatment groups (0.5, 1.0 and 2 g/kg of azodyes) and negative (150 mM NaCl) and positive (50 mg/kg of NEU) control groups. Bone marrow polychromatic (PCE) and normochromatic (NCE) erythrocytes and micronucleated PCE (MNPCE) were statistically analyzed: frequency and PCE:NCE ratio. For animal groups treated with all azodyes, analyses of the frequency of MNPCEs and PCE/NCE ratio showed significant differences between the treatment groups (0.5-2 g/kg) and the control groups (NaCl and NEU). Each azodye exhibited genotoxic and systemic toxic effects correlated to treatment dose, time of euthanasia, and sex of the animal. The data suggest potential clastogenic and/or aneugenic effects that can potentiate systemic toxic risks associated with the azodyes whether the genotoxicity be dependent on dose (ponceau 4R, sunset yellow, and tartrazine), time (ponceau 4R, red 40, and sunset yellow), or sex (red 40 and tartrazine).

Fractionated Irradiation of Right Thorax Induces Abscopal Damage on Bone Marrow Cells via TNF-α and SAA.

Radiation-induced abscopal effect (RIAE) outside of radiation field is becoming more attractive. However, the underlying mechanisms are still obscure. This work investigated the deleterious effect of thoracic irradiation (Th-IR) on distant bone marrow and associated signaling factors by irradiating the right thorax of mice with fractionated doses (8 Gy × 3). It was found that this localized Th-IR increased apoptosis of bone marrow cells and micronucleus formation of bone marrow polychromatic erythrocytes after irradiation. Tandem mass tagging (TMT) analysis and ELISA assay showed that the concentrations of TNF-α and serum amyloid A (SAA) in the mice were significantly increased after Th-IR. An immunohistochemistry assay revealed a robust increase in SAA expression in the liver rather than in the lungs after Th-IR. In vitro experiments demonstrated that TNF-α induced SAA expression in mouse hepatoma Hepa1–6 cells, and these two signaling factors induced DNA damage in bone marrow mesenchymal stem cells (BMSCs) by increasing reactive oxygen species (ROS). On the other hand, injection with TNF-α inhibitor before Th-IR reduced the secretion of SAA and attenuated the abscopal damage in bone marrow. ROS scavenger NAC could also mitigated Th-IR/SAA-induced bone marrow damage in mice. Our findings indicated that Th-IR triggered TNF-α release from lung, which further promoted SAA secretion from liver in a manner of cascade reaction. Consequently, these signaling factors resulted in induction of abscopal damage on bone marrow of mice.

Toxicological assessment of Opuntia dillenii (Ker Gawl.) Haw. cladode methanol extract, fractions and its alpha pyrones: Opuntiol and opuntioside

Ethnopharmacological relevanceThe edible plant Opuntia dillenii (Ker Gawl.) Haw. commonly known as Nagphana, belongs to the Cactaceae family. It is traditionally used to treat various ailments including inflammation, gastric ulcers, diabetes, hepatitis, asthma, whooping cough and intestinal spasm. Aim of the studyDespite its traditional use in various countries, detailed toxicological studies of O. dillenii cladode are few. Thus in the current study, toxicity of O. dillenii cladode derived methanol extract, fractions and its α-pyrones: opuntiol and opuntioside have been addressed. MethodsThe test agents were assessed using both in vitro and in vivo toxicity assays. MTT on human embryonic kidney cell line (HEK-293), tryphan blue exclusion in rat neutrophils, Cytokinesis-B block micronucleus (CBMN) in human lymphocytes and genomic DNA fragmentation using agarose gel electrophoresis were performed. In acute toxicity test, mice orally received extract (5 g/kg) for 7 days followed by measurements of relative organ weight, biochemical (blood profile, liver and kidney function test) and histological studies (liver and kidney) were carried out. Rat bone marrow micronucleus genotoxicity assay was also conducted. ResultsO. dillenii derived test agents were non-cytotoxic and had no effect on the integrity of DNA. Methanol extract (5 g/kg) orally administered in mice did not cause any significant change in relative organ weights, biochemical parameters and liver and kidney histology as compared to vehicle control. In parallel, extract did not stimulate micronuclei formation in rat bone marrow polychromatic erythrocytes. ConclusionThese results led to conclude that edible O. dillenii extract is non-toxic via the oral route and appears to be non-cyto-, hepato-, nephro- or genotoxic, thereby supporting its safe traditional use against various ailments. Therefore, opuntiol and opuntioside may serve as lead compounds in designing new drug(s) derived from edible plants.

2,4-Decadienal does not induce genotoxic effects in in vivo micronucleus studies

2,4-Decadienal (E,E-) occurs naturally in foods and is also used as a flavoring ingredient. In vivo micronucleus studies were used to evaluate the potential for 2,4-decadienal to cause genotoxic effects. Male Han Wistar rats were dosed either by intraperitoneal injection or by gavage in two independent studies. The animals (12/group) received 25, 50, or 100 mg/kg bw of 2,4-decadienal via intraperitoneal injection, or 350, 700, or 1400 mg/kg bw via gavage. Dose-dependent decreases in the percentages of peripheral blood reticulocytes were observed in both studies, indicating that the target tissue was exposed to toxic levels of 2,4-decadienal. No induction of micronuclei in the bone marrow polychromatic erythrocytes or the peripheral blood reticulocytes was observed in either study. These results, coupled with previous mutagenicity studies, support the overall conclusion that 2,4-decadienal does not present a concern for genotoxicity.

Genotoxic evaluation of sibipiruna extract (Caesalpinia peltophoroides Benth) in vivo

Caesalpinia peltophoroides Benth, popularly known as sibipiruna or false brazilwood, is an ornamental species with wood potential and with great distribution in Brazil. The genus Caesalpinia has more than five hundred species, most of which have not yet been studied for their pharmacological potential. Several species of the genus are known for their antioxidant and anti-inflammatory activities. The aim of the present study was to evaluate the effect of sibipiruna leaf and flower extract on Cyclophosphamide (CP) induced DNA damage in male Swiss mice using the micronucleus test. The animals (6 animals/group) were treated for 15 consecutive days with ethanolic extract (via gavage) and on day 15 received intraperitoneally NaCl (0.9%) or CP (25 mg/Kg), being sacrificed 24 hours after treatment to evaluate the frequency of micronucleated polychromatic erythrocytes (MNPCEs). The results showed that pretreatment with the ethanolic flower extract and sibipiruna leaf, under the conditions tested, did not reduce the frequency of MNPCEs induced by CP when compared to the positive control group. However, the groups treated only with the ethanolic extract of sibipiruna flowers and leaves significantly increased the frequency of micronuclei in polychromatic erythrocytes of bone marrow when compared to the negative control group, thus demonstrating a possible mutagenic effect. Therefore, it was possible to verify that sibipiruna did not present antimutagenic activity, but a plant with possible mutagenic action in the concentrations used.

Antioxidant and genoprotective properties of the antidiabetic drug metformin under X-ray irradiation

The antioxidant and genoprotective properties of the antidiabetic drug metformin have been investigated. The research was performed both in vitro and in vivo under X-ray irradiation. It was shown that metformin reduced the formation of hydroxyl radicals and hydrogen peroxide in aqueous solutions irradiated with X-rays. The particular features of its influence on the formation of H2O2 were revealed. It has been established that metformin reduced the formation of 8-oxoguanine, a key biomarker of oxidative damage to DNA, in vitro. The effect of metformin on the frequency of micronucleus formation in polychromatic erythrocytes of bone marrow of irradiated mice was investigated. Its genoprotective and radiomimitatory properties at different concentrations and times of application are shown.

Radioprotective effects of vitamin A against gamma radiation in mouse bone marrow cells

Radioprotectors by neutralizing the effects of free radicals, reduce the destructive effects of radiation. In this protocol article, the radioprotectory effect of vitamin A on micronuclei induced by gamma radiation was evaluated using micronucleus test. Vitamin A was injected intraperitoneally at 100 and 400 mg/kg two hours before 2 Gray (Gy) of gamma radiation. Animals were sacrificed after 24 h, and then specimens of the bone marrow were smeared and stained. The number of micronuclei were counted in polychromatic cells. Both dosage of vitamin A reduced the micronucleus in bone marrow polychromatic erythrocytes (MnPCE) level, which is statistically significant. The appropriate amount of vitamin A for protection in mice is 100 mg/kg, which protect the bone marrow of mice against clastogenic effects of radiation. The results of the study showed that vitamin A, possibly with an antioxidant mechanism, eliminates the effects of free radicals from ionizing radiation on bone marrow cells and reduces genetic damage.•The data of radioprotective effects of vitamin A showed that administration of 100 mg/kg vitamin A to mice prior to 2 Gy of gamma radiation has reduced the micronucleus levels in PCE cells by a factor of 2.62.•Administration of 100 mg/kg vitamin A, which is much smaller than LD50 of vitamin A (LD50 for intraperitoneal injection = 1510 ± 240 mg/kg) can protect mice.•Vitamin A reduces the harmful effects of ionizing radiation on DNA, due to the antioxidant activity and the trapping of free radicals produced by radiation, and diminish the genetic damage caused by radiation.•Vitamin A has no effect on the proliferation and differentiation rate of bone marrow cells.

Генотоксичность модельных комбинаций действующих веществ пестицидов в тестах на бактериях Salmonella typhimurium и эритроцитах костного мозга мышей in vivo

Studies of the impacts of pesticide mixtures on non-target organisms and public health are important in view of the increasing number of pesticide formulations containing two or more active substances entering the market, the widespread use of pesticides in tank mixtures, as well as multiple treatments of crops with formulations of compounds belonging to different chemical classes. Objective. To evaluate the genotoxic activity of combinations of technical grade active ingredients of pesticides. Material and methods. The genotoxicity of 4 combinations of technical grade products of pesticide active ingredients (generics) was evaluated: thiamethoxam/triticonazole; glyphosate/cypermethrin; ethofumesate/phenmedipham/desmedipham; imidacloprid/imazalil/thiabendazole/tebuconazole using the bacterial reverse mutation test (Ames test) and the cytogenetic test based on the assessment of micronucleus incidence in mouse bone marrow polychromatic erythrocytes in vivo. Results. The combinations of thiamethoxam/triticonazole, glyphosate/cypermethrin and imidaclo-prid/imazalil/thiabendazole/tebuconazole did not show mutagenic activity in the Ames test and clas-togenic and aneugenic effects on the erythrocytes of mammalian bone marrow, which indicates their genetic safety. A mixture of ethofumesate, phenmedipham and desmedipham caused a weak genotoxic effect, inducing a dose-dependent, statistically significant increase in the incidence of micronucleated polychromatic erythrocytes in the mouse bone marrow. Discussion. The observed genotoxic activity in vivo may be due to the synergistic effect of ethofumesate, phenmedipham and desmedipham, as each of the technical grade active ingredients separately did not induce micronuclei at doses up to 2000 mg/kg body weight. Conclusion. Studies have confirmed the genetic safety of the three assessed pesticide combinations. However, some technical products of the active ingredients of analogous (generic) pesticides included in the same formulation and non-genotoxic individually may have a genotoxic effect in combination. The data indicate the importance of testing not only individual active ingredients but also their combinations.

Assessment of the Pig-a, micronucleus, and comet assay endpoints in rats treated by acute or repeated dosing protocols with procarbazine hydrochloride and ethyl carbamate.

The utility and sensitivity of the newly developed flow cytometric Pig-a gene mutation assay have become a great concern recently. In this study, we have examined the feasibility of integrating the Pig-a assay as well as micronucleus and Comet endpoints into acute and subchronic general toxicology studies. Male Sprague-Dawley rats were treated for 3 or 28 consecutive days by oral gavage with procarbazine hydrochloride (PCZ) or ethyl carbamate (EC) up to the maximum tolerated dose. The induction of CD59-negative reticulocytes and erythrocytes, micronucleated reticulocytes in peripheral blood, micronucleated polychromatic erythrocytes in bone marrow, and Comet responses in peripheral blood, liver, kidney, and lung were evaluated at one, two, or more timepoints. Both PCZ and EC produced positive responses at most analyzed timepoints in all tissue types, both with the 3-day and 28-day treatment regimens. Furthermore, comparison of the magnitude of the genotoxicity responses indicated that the micronucleus and Comet endpoints generally produced greater responses with the higher dose, short-term treatments in the 3-day study, while the Pig-a assay responded better to the cumulative effects of the lower dose, but repeated subchronic dosing in the 28-day study. Collectively, these results indicate that integration of several in vivo genotoxicity endpoints into a single routine toxicology study is feasible and that the Pig-a assay may be particularly suitable for integration into subchronic dose studies based on its ability to accumulate the mutations that result from repeated treatments. This characteristic may be especially important for assaying lower doses of relatively weak genotoxicants. Environ. Mol. Mutagen. 60:56-71, 2019. © 2018 Wiley Periodicals, Inc.

Evaluation of the Genotoxicity of a Gelidium elegans Extract in Vitro and in Vivo

Gelidium elegans is an edible red seaweed that exhibits a broad range of biological activities. However, there is little known about its genotoxic effects. The aim of this study was to evaluate the genotoxicity of a Gelidium elegans extract in three independent genotoxic tests, including a bacterial reverse mutation test, a micronucleus test, and a chromosomal aberration test. For five different strains of bacteria, the bacterial reverse mutation showed no increased reverse mutation upon exposure to 5000 μg/plate of the Gelidium elegans extract. Moreover, the frequency of micronucleated bone marrow polychromatic erythrocytes (MNPCE) was not altered by the Gelidium elegans extract. Likewise, the chromosome aberration did not increase in response to the Gelidium elegans extract. Together, these genotoxicity assessment studies suggest that Gelidium elegans extract could be considered a safe dietary ingredient.

Toxicological evaluation of two novel bitter modifying flavour compounds: 3-(1-((3,5-dimethylisoxazol-4-yl)methyl)-1 H -pyrazol-4-yl)-1-(3-hydroxybenzyl)imidazolidine-2,4-dione and 3-(1-((3,5-dimethylisoxazol-4-yl)methyl)-1 H -pyrazol-4-yl)-1-(3-hydroxybenzyl)-5,5-dimethylimidazolidine-2,4-dione

A toxicological evaluation of two novel bitter modifying flavour compounds, 3-(1-((3,5-dimethylisoxazol-4-yl)methyl)-1H-pyrazol-4-yl)-1-(3-hydroxybenzyl)imidazolidine-2,4-dione (S6821, CAS 1119831-25-2) and 3-(1-((3,5-dimethylisoxazol-4-yl)methyl)-1H-pyrazol-4-yl)-1-(3-hydroxybenzyl)-5,5-dimethylimidazolidine-2,4-dione (S7958, CAS 1217341-48-4), were completed for the purpose of assessing their safety for use in food and beverage applications. S6821 undergoes oxidative metabolism in vitro, and in rat pharmacokinetic studies both S6821 and S7958 are rapidly converted to the corresponding O-sulfate and O-glucuronide conjugates. S6821 was not found to be mutagenic or clastogenic in vitro, and did not induce micronuclei in bone marrow polychromatic erythrocytes in vivo. S7958, a close structural analog of S6821, was also found to be non-mutagenic in vitro. In short term and subchronic oral toxicity studies in rats, the no-observed-adverse-effect-level (NOAEL) for both S7958 and S6821 was 100 mg/kg bw/day (highest dose tested) when administered as a food ad-mix for either 28 or 90 consecutive days, respectively. Furthermore, S6821 demonstrated a lack of maternal toxicity, as well as adverse effects on fetal morphology at the highest dose tested, providing a NOAEL of 1000 mg/kg bw/day for both maternal toxicity and embryo/fetal development when administered orally during gestation to pregnant rats.

Genotoxic and cytostatic effects of 6-pentadecyl salicylic anacardic acid in transformed cell lines and peripheral blood mononuclear cells

In Mexico, as in many other countries, traditional medicine is used for the treatment of several diseases. In particular, Amphipterygium adstringens infusion is used for gastritis, gastric ulcers, and gastric cancer. Extracts from this tree have microbicidal effects against Helicobacter pylori, an important risk factor for gastric cancer development. Anacardic acids are constituents of A. adstringens, and 6-pentadecyl salicylic acid (6-PSA) is the most abundant. However, there is a lack of information regarding the effects of 6-PSA on cancer cells. Therefore, we investigated whether 6-PSA has differential effects on the induction of genotoxicity, cytostaticity, and apoptosis in normal human peripheral blood mononucleated cells (PBMCs), bone marrow polychromatic erythrocytes of Balb/c mice, and human transformed cell lines derived from both gastric cancer (AGS cells) and leukaemia (K562 cells).Treatment with 6-PSA (30–150μM) reduced the viability of AGS and K562 cells together with a moderate, but significant, increase in the frequency of micronucleated cells and the induction of DNA breakage (Comet Assay). Moreover, 6-PSA increased the apoptosis rate in both the AGS and K562 cell lines in a caspase 8-dependent manner. In contrast, neither cytotoxicity nor genotoxicity were observed in PBMCs or bone marrow polychromatic erythrocytes of Balb/c mice after treatment with low doses of 6-PSA (0.2–2.0mg/Kg). Instead, 6-PSA treatment resulted in the inhibition of PBMC proliferation, which was reversible after the compound was removed. Additionally, 6-PSA treatments (2–20mg/Kg) increased the frequency of mature polychromatic erythrocytes in the bone marrow, suggesting a possible effect on the differentiation process of immune cells.The present results indicate that 6-PSA induces cytotoxicity and moderate genotoxicity, together with an increase in the apoptosis rate, in a caspase 8-dependent manner in gastric cancer cells. In contrast, a low toxicity was observed when PBMCs were exposed to 6-PSA.

Impact of antidepressant drug clomipramine on hepatorenal functions, lipid peroxidation, micronucleus frequencies and DNA damage using an alkaline comet assay

Clomipramine is a tricyclic antidepressant commonly used to treat anxiety related behavioral disorders in human and animals. The current study was performed to assess the effects of different therapeutic doses of clomipramine hydrochloride supplementation on hepato-renal functions, lipid peroxidation, frequencies of micronucleated polychromatic erythrocytes in bone marrow, DNA damage in peripheral blood lymphocytes using the comet assay and effects of withdrawal of the drug for 4 weeks on these parameters. Forty two Swiss albino male mice were divided into seven equal groups. The first group served as a control, while groups 2, 4 and 6 were orally treated with low (75 mg/kg of body weight), medium ((100 mg/kg of body weight), and high doses (250 mg/kg of body weight), respectively of clomipramine hydrochloride daily for 30 days. However, groups 3, 5 and 7 served as low, medium and high withdrawal groups for 4 weeks. Different therapeutic doses of clomipramine hydrochloride resulted in a significant increase in the levels of serum ALT, AST, BUN and creatinine compared to control. Also, plasma malondialdehyde levels showed were significant increase in mice treated by different therapeutic doses of clomipramine hydrochloride compared to control group. In contrast, the administration of clomipramine hydrochloride in three therapeutic doses for 30 days caused a significant increase in the frequency of micronuclei in polychromatic erythrocytes of mice bone marrow, this increase reached more than ten times the value determined before the treatment and directly proportional to the dose. The level of basal endogenous DNA damage measured as the mean of percentage of DNA in the tail of the lymphocytes in mice treated by low, medium and high therapeutic doses of clomipramine hydrochloride were significantly higher than in controls. However, in the recovery period (4weeks), a significant amelioration in all studied parameters was observed. Results indicated that, clomipramine is a hepato renal toxic drug and induces a significant amount of DNA damage and in-vivo genotoxic drug.

Isoliquiritigen enhances the antitumour activity and decreases the genotoxic effect of cyclophosphamide.

The aim of this study was to evaluate the antitumour activities and genotoxic effects of isoliquiritigenin (ISL) combined with cyclophosphamide (CP) in vitro and in vivo. U14 cells were treated with either of ISL (5–25 μg/mL) or CP (0.25–1.25 mg/mL) alone or with combination of ISL (5–25 μg/mL) and CP (1.0 mg/mL) for 48 h. The proliferation inhibitory effect in vitro was evaluated by MTT and colony formation assays. KM mice bearing U14 mouse cervical cancer cells were used to estimate the antitumour activity in vivo. The genotoxic activity in bone marrow polychromatic erythrocytes was assayed by frequency of micronuclei. The DNA damage in peripheral white blood cells was assayed by single cell gel electrophoresis. The results showed that ISL enhanced antitumour activity of CP in vitro and in vivo, and decreased the micronucleus formation in polychromatic erythrocytes and DNA strand breaks in white blood cells in a dose-dependent way.

Effects of long term low dose acrylamide exposure on rat bone marrow polychromatic erythrocytes

I investigated whether long term low dose exposure to acrylamide increased micronucleus frequency in rat bone marrow polychromatic erythrocytes (PCEs). Twenty-five male and 25 female Wistar rats were used. Animals of each sex were segregated into two treatment groups and one control group. Each treatment group consisted of ten animals and each control group consisted of five animals. Acrylamide, 2 or 5 mg/kg/day, was administered to the treatment groups in their drinking water for 90 days. Twenty-four hours after the last treatment, bone marrow samples were obtained and analyzed for the frequency of micronucleated polychromatic erythrocytes (MNPCEs). The cytotoxic effect of acrylamide on bone marrow also was tested by assessing the polychromatic erythrocyte/normochromatic erythrocyte (PCE/NCE) ratio. Both doses of acrylamide significantly increased the frequency of MNPCEs in both male and female rats. Acrylamide also decreased the PCE/NCE ratio in both sexes compared to the control group. My study showed that chronic low dose exposure to acrylamide increased the formation of micronuclei in PCEs of male and female rat bone marrow.

Experimental Research on the In Vitro Antitumor Effects of Crataegus sanguinea

Crataegus sanguinea is a wild plant, which has been widely grown in the north and south of the Tianshan mountains in Xinjiang. In order to explore their anti-cancer properties, edible wild plants from Xinjiang have been tested for their antitumor properties. We used Ames tests, mouse bone marrow polychromatic erythrocytes micronucleus tests, and tumor cells cultured in vitro to study the anti-mutagenic and anti-tumor effects of C. sanguinea extract. We have shown that C. sanguinea has anti-mutagenic effect, but no mutagenicity. Cell culture in vitro experiments show that there is no inhibition of growth or increase in cell death on normal mouse fibroblasts, but a stronger inhibition of cell growth and an increase in cell death of Hep-2 and MGC-803 tumor cells. The results of this study illustrate that C. sanguinea extract has both anti-mutagenic and anti-tumor effects.

Influence of medicinal natural mineral waters of Sinyak’s and Golubinsk’s deposits on radiation induced geno- and cytotoxic effects in experimental animals

Effects of medicinal mineral water from the borehole 4-E of Golubinsk’s and borehole 15-PE of Sinyak’s on the formation of cyto- and genotoxicity in bone marrow cells of experimental animals after course treatment and action of ionizing radiation is investigated. It is found that the treatment of mineral water did not effect the frequency of micronucleus in polychromatic erythrocytes of bone marrow and essentially decreased their level after irradiation 
 

The effect of different acute concentrations of cadmium chloride on the frequency of micronuclei in AO rats

Cadmium (Cd) is highly toxic heavy metal which may cause severe biological effects in vivo and in vitro. In this study, an evaluation of the acute Cd ability to trigger micronuclei (MNi) formation was carried out on 3-monthold male and female Albino Oxford (AO) rats using micronucleus (MN) test. Experimental animals were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight. Control animals received equal volume of sterile phosphate buffered saline. The results showed that 2 mg CdCl2 per kg b.w. concentration caused a highly statistically significant (P < 0.001) increase in MNi formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a clear-cut concentration-dependent effect. Lower concentrations of CdCl2 used (0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical significance. Sex differences in MNi production in bone marrow PCEs after acute exposure to different experimental concentrations of CdCl2 were not observed in our study. Our results indicate the ability of CdCl2 to exerts genotoxic effects in bone marrow of AO rats, and complement previous data on the genotoxicity of this important environmental contaminant, burdening the body from different sources - major being industrial exposure, food and cigarette smoking.

Mutagenic Effects of Carboplatin on Somatic Cells of Male Albino Mice

Carboplatin is one of the anticancer drugs used in chemotherapy for cancer patients. Cytogenetic effects were evaluated by employing bone marrow micronucleus test, one of the short-term tests that adopted in such studies. Micronucleus test was used by injecting the mice with the three doses for five consecutive days into the intraperitoneal injection. Toxicity of carboplatin was observed in stimulates the production of micronucleus in polychromatic erythrocytes of bone marrow of treated mice. A dose-response relationship was observed giving evidence that carboplatin is positive clastogene.