Bone Marrow Extract Research Articles

Core Decompression with Bone Marrow Aspirate Concentrate Implantation in Osteonecrosis of the Femoral Head with a Minimum of 2-year Follow-up - A Pilot Study.

Osteonecrosis of the femoral head (ONFH), resulting from impaired blood supply to the head of the femur, presents a significant challenge to clinicians due to its debilitating nature. Conservative treatment often offers insufficient pain relief and debilitating functional outcomes which necessitate alternative therapies. Bone marrow aspirate concentrate (BMAC), a potent orthobiologics and rich in mesenchymal stromal cells and growth factors, holds good promise as the minimally invasive procedure for ONFH. With the preceding research suggesting clinical and functional efficacy, we assessed the therapeutic effectiveness of BMAC in ONFH management in joint preservation. A prospective cohort study was conducted with 20 patients suffering from ONFH who failed to respond to 6 months of conservative treatment. A uniform surgical procedure was performed by a single surgeon, involving bone marrow extraction from the anterior iliac crest and subsequent processing into an 8-10 mL of BMAC concentrate. The BMAC was then injected into the implanted into the decompressed femoral head. The post-operative protocol comprised weight-bearing mobilization, physiotherapy, and a 4-week NSAID-free regimen. Outcome measures included pain scores, hip function, knee symptoms, sports activities, patient satisfaction, and recommendation of the procedure. Of the 20 patients suffering from ONFH, primarily the left side, most of whom were at stage 2b, significant pain reduction and functional improvement were observed over 24 months. The mean pain score decreased from 9.00 to 3.55, while the hip function score increased from 46.12 to 88.60. However, some patients encountered complications such as symptom recurrence (5%), disease progression (10%), and persistent pain (5%). Core decompression with BMAC implantation emerges as a promising, effective, and safe treatment for ONFH with better costeffectiveness and minimal side effects, making it a feasible treatment alternative.

A method for bone marrow extraction of diatoms for forensic science applications.

Diatoms show potential as trace evidence indicators, particularly as evidence of drowning. Often, the diatom test to diagnose drowning is done on soft tissue or bone marrow from a recently deceased individual. This method presented here combines elements from previous forensic literature and methods of diatom isolation in phycology to extract diatoms from bone marrow of skeletal remains for forensic use. This diatom extraction method is time-efficient, minimizes contamination risk, and produces samples of intact diatoms. This method is designed to complete sample preparation within 24 h, sampling the bone for diatoms internally and externally. This method was developed using porcine long bones submerged in water with live diatoms for up to 3 months. Three marrow samples were extracted from each bone so the method was developed using 102 marrow samples. Additionally, 132 surficial bone and environmental samples were collected and prepared during method development. To briefly summarize the method, the bone joints were cut off with an angle grinder in a biosafety hood to expose the marrow, which was removed from the hip, knee, and shaft as separate samples. The marrow was digested with nitric acid at 400°C in glass beakers before being centrifuged with DI water, plated onto microscope slides, and observed with a compound microscope. Observation found good preservation of unbroken diatom cell walls throughout the process. This method can be used to prepare diatoms as forensic trace evidence.

"Real old-timey": Storytelling and the Language of Resurgence in Cherie Dimaline's The Marrow Thieves

Through an examination of Cherie Dimaline's (Métis) The Marrow Thieves (2017), this essay investigates the ongoing reconceptualization of the young adult genre as it expands to include Indigenous texts. Dimaline's novel disrupts settler narratives of supremacy embedded in many non-Indigenous young adult narratives that rely upon the suppression of rebellious young adults, and more specifically on the absence of Indigenous young adults who threaten the settler project. In our analysis of Marrow, we consider how youth characters are empowered by practices of Indigenous resurgence that connect them with what the novel calls the "real old-timey": ways of knowing and being that were present long before the novel's settler apocalypse and that will continue long after it. Storytelling and language are a central focus of the novel and the direct means by which the characters ultimately gain the power to destroy the new residential schools and their bone marrow extraction machines. Consequently, we focus on how opposition to settler institutions and structures intensify throughout the novel as youth characters integrate themselves into their community through old-timey resurgent acts of storytelling and language.

New evidence of Neandertal butchery traditions through the marrow extraction in southwestern Europe (MIS 5-3).

Long bone breakage for bone marrow recovery is a commonly observed practice in Middle Palaeolithic contexts, regardless of the climatic conditions. While lithic technology is largely used to define cultural patterns in human groups, despite dedicating research by zooarchaeologists, for now butchering techniques rarely allowed the identification of clear traditions, notably for ancient Palaeolithic periods. In this paper, we test the hypothesis of butchery traditions among Neandertal groupsusing the bone assemblages from three sites in southwestern Europe. These sites are located in southeastern France and northern Italy and are dated to the Late Middle Palaeolithic: Abri du Maras (Marine Isotopic Stages (MIS) 4-3, Ardèche), Saint-Marcel (MIS 3, Ardèche), and Riparo Tagliente (MIS 4-3, Verona). The detection of culturally-induced patterns of bone breakage involves differentiating them from intuitively generated patterns. To tackle this issue, we used a zooarchaeological approach focusing on the percussion marks produced during the bone breakage process. Statistical analyses as the chi-square test of independence were employed to verify if percussion mark locations were randomly distributed, and if these distributions were different from the intuitive ones. For femurs and humeri, our results demonstrate that Neandertal groups occupying the Abri du Maras (levels 4.1 and 4.2) and the Saint-Marcel Cave (levels g and h) sites in France applied butchery traditions to recover yellow marrow. However, the traditions developed at each site were different. On the contrary, in Riparo Tagliente, in Italy, several groups or individuals of a same group did not share the same butchery traditions over time. Regarding the Abri du Maras and Saint Marcel Cave assemblages, our research demonstrates that Neandertal groups applied intense standardized bone breakage, far from the intuitive practice observed experimentally and related to bone density and/or skeletal morphology. These standardized patterns, which are systematic and counter-intuitive, can be interpreted as culturally induced for the Abri du Maras and Saint Marcel Cave. The diversity of Neandertal traditions should be considered by taking into account the butchery, in particular the practice of bone marrow extraction, and not only technological behaviours and types of tool kits.

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma.

Fluorescence in situ hybridization (FISH) detection is an indispensable method in genetic risk stratification in multiple myeloma (MM), which is one of the most common hematological malignancies. The identifying characteristic of MM is accumulated malignant plasma cells in bone marrow. FISH reports for MM mainly focus on purified or identified clonal plasma cells, rather than all nucleated cells, by sorting with anti-CD138 magnetic beads or marking with cytoplasmic immunoglobulin light chain κ or λ. Bone marrow interphase nuclei are usually obtained from fresh bone marrow cells. However, satisfactory enrichment of plasma cell specimens requires large amounts of fresh heparin anti-coagulated bone marrow, which cannot be obtained in the case of difficult bone marrow extraction or a bone marrow dry tap. Herein, we establish a novel method to improve the success of FISH detection on stained or unstained bone marrow smears. Bone marrow smears are easier to obtain than anticoagulated bone marrow specimens.

A way to break bones? The weight of intuitiveness.

During the Paleolithic period, bone marrow extraction was an essential source of fat nutrients for hunter-gatherers especially throughout cold and dry seasons. This is attested by the recurrent findings of percussion marks in osteological material from anthropized archaeological levels. Among them some showed indicators that the marrow extraction process was part of a butchery cultural practice, meaning that the inflicted fracturing gestures and techniques were recurrent, standardized and counter-intuitive. In order to assess the weight of the counter-intuitive factor in the percussion mark pattern distribution, we carried out an experiment that by contrast focuses on the intuitive approach of fracturing bones to extract marrow, involving individual without experience in this activity. We wanted to evaluate the influence of bone morphology and the individuals' behaviour on the distribution of percussion marks. Twelve experimenters broke 120 limb bones, a series of 10 bones per individual. During the experiment, information concerning the fracture of the bones as well as individual behaviour was collected and was subsequently compared to data from the laboratory study of the remains. Then, we applied an innovative GIS (Geographic Information System) method to analyze the distribution of percussion marks to highlight recurrent patterns. Results show that in spite of all the variables there is a high similarity in the distribution of percussion marks which we consider as intuitive patterns. The factor influenced the distribution for the humerus, radius-ulna and tibia series is the bone morphology, while for the femur series individual behaviour seems to have more weight in the distribution. To go further in the subject we need to compare the intuitive models with the distributions of percussion marks registered in fossil assemblages. Thus, it would be possible to propose new hypotheses on butchering practices based on the results presented in this work.

Urine Cells-derived iPSCs: An Upcoming Frontier in Regenerative Medicine.

There is a momentous surge in the development of stem cell technology, such as therapeutic and diagnostic tools. Stem cell-derived cells are currently used in various clinical trials. However, key issues and challenges faced involve the low differentiation efficiency, integration and functioning of transplanted stem cells-derived cells. Extraction of bone marrow, adipose or other mesenchymal stem cells (MSCs) involves invasive methods, specialized skills and expensive technologies. Urine-derived cells, on the other hand, are obtained by non-invasive methods; samples can be obtained repeatedly from patients of any age. Urine-derived cells are used to generate reprogrammed or induced pluripotent stem cells (iPSCs) which can be cultured and differentiated into various types of cell lineages for biomedical investigations and drug testing in vitro or in vivo using model animals of human diseases. Urine cells-derived iPSCs (UiPSCs) have emerged as a major area of research having immense therapeutic significance. Given that preliminary preclinical studies are successful in terms of safety and as a regenerative tool, the UiPSCs will pave the way to the development of various types of autologous stem cell therapies.

P-EP013. Superior migration propensity of dental pulp stem cells in in vitro and in vivo models of excitotoxic neurodegeneration

Introduction. Mesenchymal stem cell therapy has gained colossal attention as a therapeutic alternative in treating neurodegenerative diseases (ND). The challenges in cell-based therapy comprises of defining a suitable cell type expressing homing factors, the precise injection/administration timing based on blood brain barrier (BBB) dynamics and the route of administration that would aid in functional recovery. Even though, bone marrow mesenchymal stem cells (BM-MSC) were extensively investigated for their role in treating spectrum of diseases, the limitations like painful bone marrow extraction, modest yield and poor CNS engraftment following systemic injections prompt to search for an alternate source of MSCs. We hypothesize that neural crest derived dental pulp stem cells (DPSC) would be a better candidate for treating ND due to its ease isolation, higher yield and for their inherent propensity towards neural lineage. We investigated the migratory potential of DPSC vs BM-MSC in in- vitro and in vivo models of kainic acid (KA) induced neurodegeneration. Methods. Trans-well, matrigel, and chorioallantoic membrane (CAM) migration assays were carried out in an in vitro model of neurodegeneration. We assessed the CNS engraftment potential of DPSC/BM-MSC following intravenous administration in animal model of KA mediated hippocampal neurodegeneration and also studied the role of BBB damage after excitotoxicity to facilitate efficient homing of DPSC/BM-MSC. Functional recovery was assessed by a battery of behavioural tests after stem cell injection. Results. Following KA treatment to neurons in vitro, the migration potential of DPSC towards the degenerating site was significantly higher than BM-MSC. To validate the in vitro observations, KA administration resulted in BBB damage and intravenous administration of DPSC in synchronization with KA mediated BBB damage resulted in prominent CNS engraftment and behavioral recovery compared to BM-MSC. Conclusion. Neural crest originated DPSC might possess better CNS engraftment following systemic injection.

Metabolic Changes in Aging Bone Marrow

Introduction: Aging is associated with complex molecular changes at the cellular level. Bone marrow undergoes distinct phenotypic changes with aging, the mechanisms of which are not completely understood. Current metabolomic approaches provide a comprehensive analysis of tissue-related changes that occur due to exposure to external agents, environmental shifts, or the passage of time. A limited number of studies have investigated the metabolome in aging models and have shown significant alterations in lipid and amino acid metabolism. However, no study has investigated the metabolome of aging bone marrow. In this study, we comprehensively characterized the metabolome and transcriptome of aging murine bone marrow and compared it with bone marrow from young healthy mice and chemotherapy treated mice; chemotherapy treatment is known to induce age-related changes in hematopoiesis.Methods: Mice experiment: Twenty 8-week old and eleven 18-week old BALB/c mice were used in this experiment. 8-week old mice were injected with 0.15mg/g of cyclophosphamide (chemo group, N=10) or saline (young group, N=10) intraperitoneally every 28 days, for a total of three cycles. During the same period, the 18-month old mice (N=11) were observed in similar vivarium conditions. 28 days after the last chemotherapy/saline treatment, all mice were sacrificed (after 16 hours of fasting) and bone marrow was extracted from tibia, femur, and spine. One chemotherapy-treated mouse died before bone-marrow extraction. Subsequently, red blood cells were depleted using red blood cell lysis buffer. Metabolon assay: A 200 ul aliquot of the cells in suspension were used for RNA extraction and the remaining cells were used for the metabolomic assay (Metabolon, Durham, NC, USA). For the metabolomic analysis, the bone marrow cells were pelleted (25 ul), snap frozen and stored at -80°C until the assay was performed. Statistical analysis of the metabolon assay: A one-way ANOVA followed by Tukey's test was used to test the null hypothesis of no difference in the mean values of each metabolite between experimental-groups. A P < 0.05 was used as the criteria to reject the null hypothesis in each analysis. IBM SPSS StatisticsTM ver. 24 was used to perform ANOVA and Tukey analyses. Outlier detection was determined using a median-based technique in R-package Rallfun-v27 23 and RStudio 1.0.44. RNA sequencing: Three samples from each experimental-group were used for RNA-sequencing. RNA was isolated from frozen bone marrow and 250 nanograms was used to generate RNA-sequencing libraries using TruSeq stranded mRNA library prep kit. Sequencing was performed on an Illumina NextSeq500 system that produced paired-end 75bp reads¾ an estimated 33 million reads per sample. Statistical analysis of RNA sequencing: Pair-wise-differential expression between treatment-groups (aged and chemotherapy-treated) and control (young) was determined with a limma-based general linear model using Edge-R software. Genes with a P <0.05 and FDR <0.05 were considered differentially expressed. Genes with a P <0.05, regardless of FDR score, were considered marginally differentially expressed. Pathway analysis was performed using Ingenuity Pathway Analysis (IPA). Statistically significant pathways were identified using a Fisher's exact test. Pathways with a P < 0.05 were considered significantly activated. IPA was also used to predict regulator genes and their downstream target molecules that were differentially expressed in our dataset.Results: Our results revealed broad changes in lipids, amino acids, and nucleotides in aging marrow tissue. In particular, the metabolome of the aging bone marrow exhibited a signature of suppressed fatty-acid oxidation: accumulation of free fatty acids, reduced acyl-carnitines and low β-hydroxy butyric acid. The aged bone marrow also exhibited a significant reduction in amino acid and nucleic acid pool. The transcriptome of the aging bone marrow revealed a signature of oxidative stress, known to be associated with mitochondrial dysfunction. Lastly, the metabolic and transcriptomic profiles of the bone marrow of chemotherapy treated mice did not show broad age-related changes but rather mostly resembled young healthy mice, suggestive of a lack of ‘metabolic aging’ with chemotherapy exposure. Together, these data provide significant insights into metabolic alterations associated with aging in bone marrow. DisclosuresNo relevant conflicts of interest to declare.

Promotion of Differentiating Bone Marrow Mesenchymal Stromal Cells (BMSCs) into Cardiomyocytes via HCN2 and HCN4 Cotransfection.

Aim Investigation of the influences HCN2 and HCN4 has on bone marrow mesenchymal stromal cells (BMSCs) on cardiomyocyte differentiation. Methods Miniature adult pigs were used for bone marrow extraction and isolation of BMSCs. The identification of these BMSCs was done by using flow cytometry for the detection of expressed surface antigens CD45, CD11B, CD44, and CD90. Using HCN2 and HCN4 genes cotransfected into BMSCs as group HCN2+HCN4 while myocardial induction solution was used to induced BMSC differentiation in the BMSC induction group. Myocardial marker proteins α-actin and cTnT were detected by immunofluorescence staining, while α-actin, cTnT, and Desmin myocardial marker proteins expressed were detected by Western blot. The whole-cell patch-clamp technique was used to identify and detect cellular HCN2 channels, HCN4 channel current activation curve, and the inhibitory effect of CsCl on heterologous expression currents. Results Flow cytometry results showed that CD45 and CD11B were expressed negatively while CD90 and CD44 were positive. Post HCN2 and HCN4 gene transfection, immunofluorescence staining, and Western blot showed significantly increased HCN2, HCN4, α-actin, and cTnT expressed in group HCN2+HCN4 were, which could be compared to the expression levels in the BMSC-induced group. The HCN2+HCN4 group was able to document cell membrane channel ion currents that were similar to If properties. Conclusion HCN2 and HCN4 overexpression can considerably enhance the MSC ability to differentiate into cardiomyocytes in vitro and restore the ionic current.

CHROMOSOMAL ANALYSIS OF PEROMYSCUS GRATUS (CRICETIDAE) OF TECAMACHALCO, PUEBLA, MEXICO

&lt;p&gt;The description was made of the karyotype of &lt;em&gt;Peromyscus gratus&lt;/em&gt; (Cricetidae) in the municipality of Tecamachalco, Puebla. Performing the technique of bone marrow extraction, in the obtention of chromosome, G bands was used Trypsin and for C bands a Barium Hydroxide solution was employed. &lt;em&gt;P&lt;/em&gt;. &lt;em&gt;gratus&lt;/em&gt; presents a diploid number of 2n = 48 and a fundamental number of NF = 54, the autosomes correspond to one metacentric, one submetacentric, two subtelocentric and 19 pairs telocentric from large to small. The sex chromosome X was subtelocentric and the Y was submetacentric unlike other populations of the species. The chromosomal banding pattern G was obtained. As for chromosome C banding, it was found that the constitutive heterochromatin was in the centromeric regions of the chromosomes.&lt;/p&gt;

Changes in protein profile in bone marrow extracts before and one year after gastric bypass surgery

Changes in protein profile in bone marrow extracts before and one year after gastric bypass surgery

Remarkable migration propensity of dental pulp stem cells towards neurodegenerative milieu: An in vitro analysis

Stem cell therapy provides a ray of hope for treating neurodegenerative diseases (ND). Bone marrow mesenchymal stem cells (BM-MSC) were extensively investigated for their role in neuroregeneration. However, drawbacks like painful bone marrow extraction, less proliferation and poor CNS engraftment following systemic injections of BM-MSC prompt us to search for alternate/appropriate source of MSC for treating ND. In this context, dental pulp stem cells (DPSC) could be an alternative to BM-MSC as it possess both mesenchymal and neural characteristic features due to its origin from ectoderm, ease of isolation, higher proliferation index and better neuroprotection. A study on the migration potential of DPSC compared to BM-MSC in a neurodegenerative condition is warranted. Given the neural crest origin, we hypothesize that DPSC possess better migration towards neurodegenerative milieu as compared to BM-MSC. In this prospect, we investigated the migration potential of DPSC in an in vitro neurodegenerative condition. Towards this, transwell, Matrigel and chorioallantoic membrane (CAM) migration assays were carried-out by seeding hippocampal neurons in the lower chamber and treated with 300 μM kainic acid (KA) for 6 h to induce neurodegeneration. Subsequently, the upper chamber of transwell was loaded with DPSC/BM-MSC and their migration potential was assessed following 24 h of incubation. Our results revealed that the migration potential of DPSC/BM-MSC was comparable in non-degenerative condition. However, following injury the migration potential of DPSC towards the degenerating site was significantly higher as compared to BM-MSC. Furthermore, upon exposure of naïve DPSC/BM-MSCs to culture medium derived from neurodegenerative milieu resulted in significant upregulation of homing factors like SDF-1alpha, CXCR-4, VCAM-1, VLA-4, CD44, MMP-2 suggesting that the superior migration potential of DPSC might be due to prompt expression of homing factors in DPSC compared to BM-MSCs.

Shaped stone balls were used for bone marrow extraction at Lower Paleolithic Qesem Cave, Israel.

The presence of shaped stone balls at early Paleolithic sites has attracted scholarly attention since the pioneering work of the Leakeys in Olduvai, Tanzania. Despite the persistent presence of these items in the archaeological record over a period of two million years, their function is still debated. We present new results from Middle Pleistocene Qesem Cave on the use of these implements as percussion tools. Use-wear and abundant bone and fat residues found on ten shaped stone balls indicate crushing of fresh bones by thrusting percussion and provide direct evidence for the use of these items to access bone marrow of animal prey at this site. Two experiments conducted to investigate and verify functional aspects proved Qesem Cave shaped stone balls are efficient for bone processing and provide a comfortable grip and useful active areas for repeated use. Notably, the patina observed on the analyzed items precedes their use at the cave, indicating that they were collected by Qesem inhabitants, most probably from older Lower Paleolithic Acheulian sites. Thus, our results refer only to the final phases of the life of the items, and we cannot attest to their original function. As bone marrow played a central role in human nutrition in the Lower Paleolithic, and our experimental results show that the morphology and characteristics of shaped stone ball replicas are well-suited for the extraction of bone marrow, we suggest that these features might have been the reason for their collection and use at Qesem Cave. These results shed light on the function of shaped stone balls and are consistent with the significance of animal fat in the caloric intake of Middle Pleistocene humans as shown by the archeozoological evidence at Qesem Cave and possibly beyond.

Sensory attribute and antioxidant capacity of Maillard reaction products from enzymatic hydrolysate of bovine bone marrow extract.

Maillard reaction and Maillard reaction products (MRPs) are commonly used in food industry, with various sensory and functional properties. Bovine bone marrow extract (BBME) was subjected to enzymatic hydrolysis, followed by the Maillard reaction to enhance its sensory attributes. The antioxidant and antihypertensive activities of BBME, its hydrolysate and their MRPs were assessed. (37) orthogonal experimental design was used to obtain the optimal reaction conditions for generation of MRPs with improved sensory characteristics. The optimal conditions were: processing temperature, 120°C; processing time, 60min; xylose concentration, 2.5% (w/v); cysteine concentration, 2%; Vitamin B1, 1.2%; Hydrolyzed vegetable protein, 6%; and sample to liquid ratio, 1:1. The total content of free amino acid in BBME decreased from 86.18 to 25.50gL-1 in EH-BBME-MRPs. In addition, EH-BBME and EH-BBME-MRPs contained significantly higher amount of low molecular weight peptides (< 1000Da; 47.2% and 21.84%, respectively) compared to other samples. They also exhibited enhanced antioxidant activities against DPPH, ABTS and hydroxyl free radicals, and presented improved FRAP activity and reducing power. EH-BBME-MRPs also exhibited higher antihypertensive activity compared to other samples. The results indicate that MRPs derived from BBME hydrolysate are promising components for improving food flavor and also provide health benefits.

Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders.

Mesenchymal stromal cells (MSC) have become a popular treatment modality in equine orthopaedics. Regenerative therapies are especially interesting for pathologies like complicated tendinopathies of the distal limb, osteoarthritis, osteochondritis dissecans (OCD) and more recently metabolic disorders. Main sources for MSC harvesting in the horse are bone marrow, adipose tissue and umbilical cord blood. While the acquisition of umbilical cord blood is fairly easy and non-invasive, extraction of bone marrow and adipose tissue requires more invasive techniques. Characterization of the stem cells as a result of any isolation method, is also a crucial step for the confirmation of the cells' stemness properties; thus, three main characteristics must be fulfilled by these cells, namely: adherence, expression of a series of well-defined differentiation clusters as well as pluripotency. EVs, resulting from the paracrine action of MSCs, also play a key role in the therapeutic mechanisms mediated by stem cells; MSC-EVs are thus largely implicated in the regulation of proliferation, maturation, polarization and migration of various target cells. Evidence that EVs alone represent a complex network 0involving different soluble factors and could then reflect biophysical characteristics of parent cells has fuelled the importance of developing highly specific techniques for their isolation and analysis. All these aspects related to the functional and technical understanding of MSCs will be discussed and summarized in this review.

Effects of three types of bone marrow extracts treated with different treatment methods on the taste of chicken soup.

Bone marrow extracts (BME) are good flavor enhancing substance. Three types of BME (from porcine, chicken and bovine) were treated by enzymatic hydrolysis or Maillard reaction. To explore their effects on soup taste, they were added into chicken and non-chicken soups. The contents of free amino acids, nucleotides, peptides and soluble solids in the soup were measured and the taste activity value and equivalent umami concentration value were calculated, in addition to sensory evaluation. These parameters were weighted according to a certain ratio. Most taste-active substances increased upon enzymatic hydrolysis, while reached their maximum values after Maillard reaction. In addition, the presence of chicken alleviated bitter and fatty tastes in soups added with bone extracts, thus causing the soup to be more flavorful. Sensory evaluation results revealed that umami and kokumi tastes of soups supplemented with Maillard reaction-treated bone marrow extracts were more intense.

EXPERIMENTAL SUBSTANTIATION OF THE USE OF MARAL DEER ANTLERS FOR COMBATING EXTREME PSYCHO-EMOTIONAL STRESS

This work was aimed at investigating the effect of maral antler powder on the activity of animal hematopoietic stem cells both in vivo and in vitro.For in vivo experiments based on the model of sleep deprivation, male mice of the CBA/CaLac line were used. Prior to the experiment, mice in the experimental and control groups were intragastrically administered with a water dispersion of a maral antler powder and distilled water, respectively. Subsequently, the extraction of bone marrow from the femur, cloning of erythro- and granulo-monocytopoiesis precursors and count of the number of cell colonies were performed. Experiments in vitro involved the extraction of bone marrow cells from the femur followed by their cultivation both in a culture containing a maral antler powder (experimental) and distilled water (control culture). The number of CFU was counted 7 days following the beginning of the experiment.Maral antlers are found to exhibit no noticeable modulating effect on the colony-forming activity of mouse hematopoietic stem cells in vitro. However, according to our in vivo experiments on mice, a preventive administration of an antler powder before a stressful infl uence (sleep deprivation) prevents suppression of erythropoiaesis processes, thus exhibiting a modulating effect on the activity of CFU-E and CFU-GM by increasing the number of CFU-E and reducing the number of CFU-GM by more than three times. The modulating effect of maral antlers on the activity of hematopoietic and stem cells is based on the infl uence of biologically active substances contained therein on the neuroendocrine regulation of the hematopoietic system occurring in living organisms.

Casuística de leishmaniosis visceral canina registrada en Corrientes (Argentina) durante el período 2014-2016

&lt;p&gt;Durante los meses de febrero a octubre de los años 2014 a 2016 se investigó el padecimiento de leishmaniosis visceral en caninos atendidos en la Facultad de Ciencias Veterinarias de Corrientes, Argentina. El estudio se efectuó a través del método indirecto (detección de anticuerpos contra el antígeno rK39 en suero de sangre venosa) y la prueba directa (extracción de medula ósea por punción en distal de costillas y observación microscópica de amastigotes). Durante los 3 años se examinaron un total de 409 pacientes. En 2014 (89 muestras) se registraron 22 casos positivos (24,7%); en 2015 (164 muestras) se detectaron 39 casos positivos (23,7%) y en 2016 (156 muestras) se revelaron 56 pacientes positivos (35,8%). En todos los casos los caninos fueron positivos a ambos métodos diagnósticos. En el total de años estudiados, la prevalencia en caninos fue de 28,06%. Se enfatiza que ante los pacientes sospechosos de padecer leishmaniosis visceral, se efectúen conjuntamente los métodos diagnósticos serológico (indirecto) y parasitológico (directo).&lt;/p&gt;

Effect of thermal treatment on aroma generation from bovine bone marrow extract during enzymatic hydrolysis

Because of less explored flavor characteristics and extensive utilization of bovine bone marrow extract (BBME) in food industry, this study was designed to focus on its flavor generation via Maillard reaction using different heating temperatures. Response surface method (RSM) was used to optimize the reaction conditions for generation of meaty flavor. Optimal conditions (time, temperature, and pH) were 1 hr, 120°C, and 4.67, respectively. Volatile and nonvolatile compounds (amino acids, nucleotides, and peptides) were identified by SPME/GC‐O‐MS and HPLC, respectively. The temperature (120°C) resulted in optimum Maillard reaction products (MRPs) , such as meaty aroma compounds (hexanal, nonanal, methional, 4‐methyl‐5‐hydroxyethyl‐thiazole, 2‐acetylthiophene, 2‐acetylfuran, etc. 302.8 ng/g) and umami taste‐active compounds (umami amino acids and <1,500 Da peptide were 2.38 g/L and 80.19%, respectively). Based on our results, the temperature (120°C) is recommended for flavor generation from enzymatic hydrolysis of BBME (EH‐BBME) through Maillard reaction. PRACTICAL APPLICATIONS: Raw bovine bone marrow (BBM) is generated in large quantities on a daily basis. BBM is considered as low added value products, and in general the industries are putting efforts into increasing their value. While being edible, still the BBM is not used for human consumption or pet food without proper processing. However, to a large extent, BBM is an excellent source of minerals, nutritive value protein, and vitamins, hence might contribute to the increasing protein global demand. Therefore, the current study was aimed to optimize the thermal reaction conditions that enhance the flavor generation from BBM extracts, and in turn will improve meat industry profitability, sustainability, and better utilization of available resources.