Objective To investigate the clinical application value of the 8-test for immunodiagnosis of human bone cystic echinococcosis (BCE). Methods A retrospective study was made in 36 cases who were suspected to be BCE in our hospital from October 1999 to September 2008. The mean age was 35.9 years with a range of 10 to 67 years, and including 19 males, 17 females. All cases accepted the 8-test im-munodiagnosis and the final diagnosis were confirmed by operation or biopsy. The dot immunogold filtration assay (DIGFA) and enzyme linked immunosorbent assay (ELISA) were used to detect the antibodies to anti-gens of EgCF, EgP, EgB, and Em2 in patients at one time in our research, the name abbreviated to the 8-test for immunodiagnosis of human bone cystic echinococcosis. The sensitivity, specificity, Youden index, positive likelihood ratio, negative likelihood ratio, positive predictive value and negative predictive value of the 8-test immunodiagnosis were calculated and recorded. Results Be confirmed by pathology, 11 of these 36 cases were diagnosed as bone hydatidosis (including 2 cases with thoracic vertebrae, 1 case with rib, 3 cases with lumbar vertebrae, 4 cases with sacrum, and 1 case with illum). The sensitivity was 90.91%, the specificity was 92.00%, the Youden index was 0.8291, the positive likelihood ratio was 11.3600, the negative likelihood ratio was 0.0988, the positive predictive value was 83.33%, and the negative predictive value was 95.83%.Conclusion Serological examinations can be considered as diagnosis and initial differentiation for BCE, the 8-test immunodiagnosis has high accurate rate for diagnosing human BEC. It's helpful for identification of BCE. Key words: Echinococcosis; Diagnosis; Serology; Spine