Animal Glue Research Articles

Bio-based composites from bagasse using carbohydrate enriched cross-bonding mechanism: A formaldehyde-free approach

In this study, cross-bonded self-binding and bone glue-bonded particleboards were manufactured from sugarcane (Saccharum officinarum L.) bagasse with different pre-treatments of particles. Six types of panels were manufactured from bagasse particles with and without bone glue. The physical, mechanical, and thermal properties of the panels were examined according to the standards. Fourier Transform Infrared (FTIR) spectroscopy and thermogravimetric analysis (TG) were performed to investigate the changes in the chemical bonds and thermal stability of the fabricated composites, respectively. It was found that cross-bonded bagasse self-binding (TC) and bone glue-bonded (T3) panels fabricated from non-boiled bagasse particles showed higher physical and mechanical properties compared to the other types of panels. Non-boiled bagasse particles with bone glue panels showed the highest mechanical properties, i.e., modulus of rupture (MOR = 26.22 MPa), modulus of elasticity (MOE = 4302 MPa), tensile strength = 8.35 MPa, and hardness = 1.72 MPa. TC and T3 panels also showed higher thermal stability compared to the other types of panels. A new peak at 3331–3334 cm−1 for the NH stretching vibration in the FTIR analysis represents the presence of bone glue in the cross-bonded particleboards. Thus, this research advances the production of formaldehyde-free bagasse particleboard, introducing the cross-bonding technique and sustainable bone glue.

Degradable Nanohydroxyapatite-Reinforced Superglue for Rapid Bone Fixation and Promoted Osteogenesis.

Bone glue with robust adhesion is crucial for treating complicated bone fractures, but it remains a formidable challenge to develop a "true" bone glue with high adhesion strength, degradability, bioactivity, and satisfactory operation time in clinical scenarios. Herein, inspired by the hydroxyapatite and collagen matrix composition of natural bone, we constructed a nanohydroxyapatite (nHAP) reinforced osteogenic backbone-degradable superglue (O-BDSG) by in situ radical ring-opening polymerization. nHAP significantly enhances adhesive cohesion by synergistically acting as noncovalent connectors between polymer chains and increasing the molecular weight of the polymer matrix. Moreover, nHAP endows the glue with bioactivity to promote osteogenesis. The as-prepared glue presented a 9.79 MPa flexural adhesion strength for bone, 4.7 times that without nHAP, and significantly surpassed commercial cyanoacrylate (0.64 MPa). O-BDSG exhibited degradability with 51% mass loss after 6 months of implantation. In vivo critical defect and tibia fracture models demonstrated the promoted osteogenesis of the O-BDSG, with a regenerated bone volume of 75% and mechanical function restoration to 94% of the native tibia after 8 weeks. The glue can be flexibly adapted to clinical scenarios with a curing time window of about 3 min. This work shows promising prospects for clinical application in orthopedic surgery and may inspire the design and development of bone adhesives.

Palaeoproteomics and microanalysis reveal techniques of production of animal-based metal threads in medieval textiles

Animal-based metal threads were largely used between the 10th and the fifteenth century, in European, Middle Eastern and Far Eastern textile productions for the decoration of textiles and cloths. They belong to a larger group of metal threads, used either as flat threads or wrapped around a fiber core, that were backed by an organic support (animal or paper). This study focuses on the medieval production of metal threads backed by an animal membrane (e.g. gut membrane), or skin. A total of 91 samples were collected from a corpus of 66 textile fragments belonging to 54 catalogued objects. The relevance and novelty of the present study is represented by the combination of proteomics, cross-section analysis, and scanning electron microscopy (SEM–EDS and SEM-µXRF). The diversity of materials and manufacturing techniques found within each typology of thread, respectively, membrane-based metal threads and skin-based metal threads, hinted at different production technologies. Membrane-based threads were found to be invariably made from cattle gut membrane, coated with gilt-silver leaves. A possible sheep glue adhesive was found in a few samples. Skin-based threads were made from either goat or sheep leather, coated with metal leaves or powder. Within the three different types of coatings identified (silver, gold and gilt-silver), gold coatings were the most represented. Goat leather threads were associated with an egg-white binder, while sturgeon glue was identified as adhesive in all sheep leather threads. Collagen glue from other species (cattle, sheep, horse) was occasionally found in mixed adhesives. In two textiles, the finding of human proteins indicates past contamination due to handling or use. The analytical results show coherence between the fabrication patterns of animal-based metal threads and their probable geographical areas of manufacture, indicating that the study of materials and techniques provide further criteria to classify and group textiles, and trace correlations between manufacturing centers within Eurasian territories.

Hygrothermal ageing of dry gelatine adhesive films: Microstructure-property relationships

Gelatine adhesives, also known as animal glues, are collagen-based water-soluble biopolymers derived from vertebrate connective tissues. One of the various fields in which gelatine adhesives are widely used is the conservation of cultural heritage such as decorated furniture and panel paintings. It is observed that, with time, the failure in these objects often occurs along the adhesive bondlines. Given the moisture and temperature sensitivity of these adhesives, obtaining knowledge of their long-term behaviour, when exposed to climate variations, is pivotal. Here, the influence of hygrothermal ageing (exposure to a combination of elevated temperature and relative humidity (RH) cycling) on the microstructure and macroscopic properties of four different types of gelatine adhesives is investigated. These adhesives were selected from different animal origins namely bovine, rabbit, and fish with different Bloom strengths. It was observed that ageing cycles interfere with the most critical structural feature of protein chains namely triple helices. A clear decay in triple helix content at the micro-scale, determined by Differential Scanning Calorimetry (DSC) and X-Ray Diffraction (XRD) techniques, was observed which had implications on the macroscopic properties of these adhesives such as reduction of strain to failure and toughness (strain energy density to failure). The rate of decay in properties was revealed to be the highest in the adhesives with the lowest triple helix content. This study provides a scientific view of microstructure-property relationships in gelatinous adhesives as a function of environmental ageing, and stipulates the underlying mechanism of the degradation of mechanical properties as the loss of structural triple helices, regardless of the animal origin.

Mechanical and moisture-related properties of selected dried tempera paints

Mechanical properties—modulus of elasticity and strain at break, water vapour sorption, and hygroscopic expansion of selected egg tempera and distemper paints were determined as a function of relative humidity (RH) filling in this way a critical gap in the knowledge required for the analysis of fracturing processes in paintings. The experimental work was made possible by the preparation of several tempera paints, mimicking the historical materials, in the form of large specimens. Lead white, azurite, and yellow ochre were selected as pigments, and egg yolk and rabbit skin glue as binding media. The water vapour sorption and the moisture-related swelling of the paints were dominated by the proteinaceous components of the binders. The linear hygroscopic expansion coefficient of the dried egg yolk binder was approximately 1 × 10−4 per 1% RH, several times less than the coefficient of the collagen glue (4 × 10−4 per 1% RH). The moduli of elasticity of egg tempera paints at the RH mid-range were comparable to the moduli of aged oil paints, whilst the modulus of elasticity of the distemper paint was close to values measured for animal glue-based grounds. The paints experienced the transition from brittle to ductile states on increasing RH. The egg tempera paints were found to be more brittle than the distemper paint, gessoes, and, generally, aged oil paints. The observations modify a frequently used laminar model of panel paintings in which the mismatch in the response of glue-based ground layer and wood substrate to variations in RH has been identified as the worst-case condition for the fracturing of the entire pictorial layer. This study demonstrated that tempera could be more brittle than the ground layer and in consequence more vulnerable to cracking.

Identification and analysis of gemstone binding materials on imperial rank belts excavated from the tomb of Murongzhi

AbstractThis paper analyzes and identifies the binders used in the jewel settings of the Baodian and Diexie belts excavated from the tomb of Murongzhi in Wuwei, Gansu, China. Fourier transform infrared spectroscopy (FTIR) indicated the presence of protein in the binder samples. The result of gas chromatography–mass spectrometry (GC‐MS) shows that samples from both contained hydroxyproline, a characteristic amino acid for animal glue. Principal component analysis was performed on one of the samples, and it was concluded that the binder was a mixture of hide glue and egg glue. Further analysis using scanning electron microscopy with energy‐dispersive X‐ray (SEM‐EDX) and laser Raman spectroscopy (Raman) found that a red particulate matter visually identified in the binder was cinnabar, which was apparently added to binder in order to modify the color of the jewels. This study provides a basis for the conservation and restoration of future finds of elite jewelry. It also shows how imperial jewelry was made during the Tang Dynasty. Finally, the analysis of these heraldic belts provides a key insight the life and role of an exiled prince in the Tang court.

Orthogonal Experimental Study on the Ratio of Similar Materials of Coal Body

According to the coal samples collected from Zhaogu No.2 Coal Mine as raw coal samples, the similar materials of coal body were studied by using river sand, pulverized coal, Portland cement and calcium carbonate. The orthogonal test method of four factors (bone glue ratio, aggregate ratio, rubber ratio, water mixing rate) and three levels was used to obtain the physical and mechanical parameters such as density, uniaxial compressive strength, elastic modulus, cohesion, internal friction angle and tensile strength of coal similar materials. The range analysis method was used to obtain the key influencing factors and variation rules of the physical and mechanical properties of coal similar materials. Finally, the method of multiple linear regression analysis was used to obtain the similar material ratio that was most consistent with the mechanical properties index of raw coal. The ratio of calcium carbonate: cement: river sand: coal powder: water = 1: 5: 0.9: 5.9: 3.2. The density error, compressive strength error, elastic modulus error and cohesion error of coal sample and raw coal are small, which proves that the similar material is similar to the mechanical properties of raw coal, and provides guidance for subsequent research.

Electrochemistry of Tin Deposition from Methanesulfonic Acid

The electrolytic refining of crude tin is generally carried out in the fluorosilicic acid (H2SiF6) system with the assistance of bone glue and β-naphthol. However, the high saturated vapor pressure and low stability of H2SiF6 present environmental concerns and do not align with sustainable development goals. In this paper, the electrochemical behavior of tin on a glassy carbon (GC) electrode was studied in a relatively green and environmentally friendly methanesulfonic acid (MSA) system. Bone glue, sodium lignosulfonate, and β-naphthol were used as additives to make the deposit morphology smooth and to ensure grain refinement. The electrochemical reduction process of Sn2+ in an MSA system is a quasi-reversible process controlled by diffusion. The apparent activation energy Ea = 14.189 kJ/mol for the ion diffusion of Sn2+ was further calculated. The results of chronoamperometry showed that the electrocrystallization of Sn2+ on the GC electrode gradually tended to three-dimensional instantaneous nucleation with the increase in applied potential. The morphology and phase of the deposits were analyzed using scanning electron microscopy (SEM) and X-ray diffraction (XRD). The results showed that the deposits were uniform and dense pure tin. This work elucidates the electrochemical behavior of tin in a methanesulfonic acid system.

Ecological glue for traditional furniture: Optimization of the handicraft for making fish glue.

Through a comprehensive review of published literature on fish glue (FG), the ecological glue for traditional furniture, the traditional handicraft for making FG was found to include six main processes: soaking, steaming, manual smashing, decoction, filtration, and airing. The handicraft that makes FG is manual and is not only time-consuming and laborious but does not have clearly documented standard processes and is thus less repeatable. Considering this, experiments to optimize a new technique for making FG were designed. Six basic technological processes (cutting and drying, crushing, soaking, decoction, filtration, and airing) were investigated to optimize the new glue production technique. The technological processes of the new technique were compared with those of the traditional handicraft method. The results indicated that preparing FG following the optimal processes of the new glue-making technique not only ensured the quality of the glue solution but also outperformed the traditional handicraft technique in the following aspects: 1) it simplifies the production process, reduces labor intensity, and saves time: the soaking time is decreased by 50% and the traditional manual smashing process is not required; 2) it improves the glue yield by 5.42%; and 3) due to introduction of mechanical processing, time and temperature are controllable, rendering production more repeatable and easily up-scaled.

The first identification of composite paints with proteinaceous binder in Upper Palaeolithic (31–23 ka) organic decorations

Research of coloring pigments and binding compounds from the Upper Palaeolithic (UP), including on portable art objects such as personal ornaments, provides new insights into social and cultural aspects of human history. However, we lack a comprehensive study of the composite pigment mixtures and binding materials that were produced intentionally and used for coloration. The study of several personal ornaments from the UP layers dated 31–23 ka calBP in Mezmaiskaya Cave, North Caucasus (Russia), shows that UP paints have a complex chemical composition. Using ATR–FTIR and SEM–EDS, we have identified that for coloring organic personal decorations UP humans used composite paint mixtures produced from organic (bitumen) and inorganic (red bolus/kaolin) natural pigments. Also, we firstly identified that UP humans applied a proteinaceous binder, likely representing a kind of the gelatine type animal glue, which they intentionally produced from animal origin materials using boiling. This is the oldest evidence of boiling placing its origin at about 30 ka within the late UP and linking this innovation to the need of producing organic binding material for dyeing rather than with the food preparation.

Preparation of alkali-activated nickel slag-based cemented tailings backfill: Workability, strength characteristics, localized deformation and hydration mechanism

To solve the problems of high magnesium nickel slag (HMNS) and tailing stacks with large storage and environmental pollution, alkali-activated HMNS-based cementitious materials were prepared by using NaOH, phosphogypsum (PG), HMNS and ground granulated blast furnace slag (GGBFS), and cemented tailings backfill (CTB) was prepared using tailings as aggregates. The workability, strength characteristics, localized deformation and hydration mechanism of CTB were studied. Based on the digital speckle correlation method (DSCM), the localized deformation of CTB was analyzed. The hydration mechanism of CTB was analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetry-differential scanning calorimetry (TGDSC), mercury intrusion porosimetry (MIP) and scanning electron microscopy and energy dispersive spectroscopy (SEMEDS). The results showed that the optimal mix proportions for the cementitious material were 8%, 30%, 6%, and 0.38 for PG content, GGBFS content, alkali dosage, and water/binder ratio, respectively. The best performance of CTB was achieved when the bone glue ratio (BGR) and mass concentration (MC) were 5:1 and 79%, respectively; the slump, slump flow, and consistency of CTB decreased continuously with increasing BGR and MC; the layering degree, bleeding rate, and linear shrinkage of CTB continuously increased with increasing BGR, and decreased with increasing MC; the unconfined compressive strength of CTB increased with increasing maintenance age and MC and decreased with increasing BGR; when the BGR was small (3:1), CTB exhibited shear failure, and when the BGR was large (6:1), CTB exhibited tensile failure. The main hydration products of CTB were C-S-H gel, M-S-H gel, ettringite (AFt), Ca(OH)2 and CaCO3. In the early stage of curing, CTB has more pores, fewer hydration products and a relatively loose internal structure, flocculation, sheet, network and needle rod hydration products coated tailing particles, porosity decreased, and CTB became dense.

PROTOTYPE OF Ag@ZnO CORE SHELL FOR THE STERILIZATION OF MURAL PAINTINGS

Cultural heritage sites often experience the unfavorable alteration of biodeterioration of mural paintings and wall inscriptions. Controlling or preventing biodegradation that may occur has been achieved through the use of various treatment methods, such as physical, chemical, and environmental control procedures. Regrettably, these treatments are often highly toxic to the health of conservators and visitors. Moreover, it causes pigmentation, is expensive, has low long-term results, and has poor long-term effectiveness. The use of Ag@ZnO core shell prototype in the field of cultural heritage was the first time in this research. The application was made to a painted wall inscription that dates back to the Middle Kingdom of ancient Egypt. A one-step chemical method was used to synthesize Ag@ZnO and it was examined using XRD, TEM, BET surface area, and Raman. These tests proved that the silver's core shell covered the zinc oxide's core and confirmed its purity. The painting on the wall was analyzed by SEN-EDX, Raman, and XRD analysis, and it was determined that the stone was limestone, the pigments were (Carbon for black pigment, Hematite for a red pigment, and Egyptian blue for blue pigment). These pigments and animal glue were combined to create the binding medium. The painted wall inscription was treated with Ag@ZnO treatments using a spray technique with a 5% concentration. The spectrophotometer was used to confirm that the color change has been below 5. This treatment method is non-hazardous, can be utilized both in vivo and in vitro, and does not necessitate the required environmental atmosphere or UV index.

Identification and tentative removal of collagen glue in Palaeolithic worked bone objects: implications for ZooMS and radiocarbon dating

Collagen glue has been used for nearly two centuries to consolidate bone material, although its prevalence in museum collections is only now becoming visible. Identifying and removing collagen glue is crucial before the execution of any geochemical or molecular analyses. Palaeolithic bone objects from old excavations intended for radiocarbon dating were first analysed using ZooMS (Zooarchaeology by Mass Spectrometry) to identify the animal species, however peaks characteristic of both cattle and whale were discovered. Two extraction methods for ZooMS were tested to identify the authentic animal species of these objects, which revealed that these were originally whale bone objects that had been consolidated with cattle collagen glue. This is the first time animal collagen glue has been identified in archaeological remains with ZooMS, illustrating again the incredible versatility of this technique. Another technique, Fourier Transform Infrared Spectroscopy in Attenuated Total Reflectance mode (FTIR-ATR), was also tested if it could rapidly identify the presence of collagen glue in archaeological bone material, which was not the case. Two other cleaning methods were tested to remove bone glue contamination prior to radiocarbon dating, along with two modified collagen extraction methods for ZooMS. These methods were applied to bone blank samples (FmC = 0.0031 ± 0.0002, (n = 219), 47 336 ± 277 yr BP) that were experimentally consolidated with collagen glue and to the Palaeolithic bone material (ca. 15 000 and 12 000 yr BP). The experimental bone blanks produced excellent 14C ages, suggesting the cleaning methods were successful, however the 14C ages for some of the Palaeolithic material remained too young considering their contextual age, suggesting that the collagen glue contamination had most likely cross-linked to the authentic collagen molecule. More research is needed in order to gain a deeper understanding of the occurrence and elimination of cross-linked collagen-based glues in material from museum collections.

Development and investigation on physical and mechanical properties of mahogany sawdust particleboards made with tannic powder of Indian tamarind (Pithecellobium Dulce Benth)

The recycling of forest residues was investigated in the present study. It arms to determine the physical and mechanical properties of particleboards from mahogany sawdust bonded by pearls bone glue or tannic powder of peel of Indian tamarind (Pithecellobium Dulce). The influence of the size of particles on the physical and mechanical properties of the particleboards is studied. Five different granulometries (raw sawdust; 2.5 < g £ 5; 1.6 < g £ 2.5 and 0.8 < g £ 1.6, and g £ 0.8) were investigated. The rate of the adhesives used were 5; 6.5; 7; 7.5; 8; 10, 12.5 and 15 percent. Physically, the density, thickness swelling (TS) and water absorption (WA) were evaluated. Mechanically, the modulus of elasticity (MOE), the modulus of rupture (MOR), the modulus of Young E, the tensile modulus of rupture (MOT) and the internal bond (IB) were determined. The results obtained showed that the granulometry has a great influence on the physical as well as the mechanical properties of the Mahogany sawdust particleboards. The pressing temperature is 160 °C. The threshold fixed by the American standard ANSI A208.1-2016 for the MOE and the MOR are widely reached. The values of the density classified the particleboards from sawdust of Mahogany wood in the categories of low and mean density. The thickness swelling and water absorption has not respectful to the American standard ANSI A208.1–2016 [1]. So, the manufactured boards can only be used in dry conditions.

A Study of Pigment, Adhesive, and Firing Temperature in Pottery Figurines Excavated from the Tomb of Qibi Ming, China

Some painted pottery figurines were excavated from the tomb of Qibi Ming of the Tang Dynasty. A series of analytical techniques were employed to understand the craftsmanship of these painted pottery figurines. The pigment, cross-section, adhesive, and firing temperature were analyzed using microscopy (OM), energy X-ray fluorescence spectrometry (EDX), micro-Raman spectroscopy, pyrolysis–gas chromatography–mass spectrometry (Py-GC/MS), and a dilatometer (DIL). The results demonstrated that the surface of the pigment layers had degraded to different degrees. The pigment particles were litharge, gypsum, malachite, cinnabar, hematite, minium, white lead, and carbon black. The cross-sectional images show that the painted layer of figurines 10-0966 and 10-0678 included a pigment layer and a preparation layer. The preparation layer of both pigments was lead white. Animal glue was used as an adhesive. The firing temperature of the pottery figurines was likely 1080 °C. This study can provide more accurate information with regard to the composition of the raw materials utilized in the making of these artifacts and support the selection of appropriate substances for the purposes of conservation and restoration of the painted pottery figurines.

The Influence of Acid Casein on the Selected Properties of Lime-Metakaolin Mortars.

One of the ways to modify selected parameters of lime mortars is the use of biopolymers of animal origin, such as bone glue, skin glue, bovine blood, eggs, and casein. These are protein-based biopolymers. Casein is an example of an organic polymer produced from cow's milk. The aim of the work was to investigate the possibilities of improving selected properties of mortars based on hydrated lime and metakaolin. The mixture was modified with powdered technical casein in amounts of 0.5%, 1.5%, 1%, 1.5%, and 2% as a partial mass replacement for the binding mixture. Additionally, the influence of increasing the amount of water on the properties of the mortar with a casein admixture of 2% was checked. This study examined consistency, shrinkage, water absorption, capillary action, porosity, flexural, compressive strength, and Young's modulus. The admixture of casein influenced the properties of the mortar, but not in all cases, and it was possible to determine a clear trend related to the variable amount of casein. Strength properties deteriorated as the amount of casein increased. When air bubbles were introduced into the mortar after the casein was dissolved, the porosity increased as the amount of admixture increased. The moisture properties improved; namely, casein led to a reduction in water absorption and water absorption caused by capillary action. No relationship was observed between the amount of casein and the drying shrinkage. Increasing the amount of water in the mixture led to the expected effects, i.e., an increase in porosity, shrinkage, and water absorption, and a decrease in mechanical strength.

High-Throughput Screening of Thiol-ene Click Chemistries for Bone Adhesive Polymers.

Metal surgical pins and screws are employed in millions of orthopedic surgical procedures every year worldwide, but their usability is limited in the case of complex, comminuted fractures or in surgeries on smaller bones. Therefore, replacing such implants with a bone adhesive material has long been considered an attractive option. However, synthesizing a biocompatible bone adhesive with a high bond strength that is simple to apply presents many challenges. To rapidly identify candidate polymers for a biocompatible bone adhesive, we employed a high-throughput screening strategy to assess human mesenchymal stromal cell (hMSC) adhesion toward a library of polymers synthesized via thiol-ene click chemistry. We chose thiol-ene click chemistry because multifunctional monomers can be rapidly cured via ultraviolet (UV) light while minimizing residual monomer, and it provides a scalable manufacturing process for candidate polymers identified from a high-throughput screen. This screening methodology identified a copolymer (1-S2-FT01) composed of the monomers 1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione (TATATO) and pentaerythritol tetrakis (3-mercaptopropionate) (PETMP), which supported highest hMSC adhesion across a library of 90 polymers. The identified copolymer (1-S2-FT01) exhibited favorable compressive and tensile properties compared to existing commercial bone adhesives and adhered to bone with adhesion strengths similar to commercially available bone glues such as Histoacryl. Furthermore, this cytocompatible polymer supported osteogenic differentiation of hMSCs and could adhere 3D porous polymer scaffolds to the bone tissue, making this polymer an ideal candidate as an alternative bone adhesive with broad utility in orthopedic surgery.

CHARACTERIZATION OF A GRECO-ROMAN STUCCO MASK (REG. NO. 229) PRESERVED AT THE NATIONAL MUSEUM OF EGYPTIAN CIVILIZATION, CAIRO, EGYPT

The Greco-Roman stucco masks are of great historical, artistic and religious importance. According to the restoration-conservation strategies, the detailed mineral characterization of artifacts is an important step that facilitates the selection of appropriate restoration procedures. Different characterization techniques such as USB digital microscope, scanning electron microscopy–energy dispersive X-ray analysis, X-ray diffraction analysis (XRD), thermal analysis (DTA/DTG), Fourier transform infrared and micro-Raman spectroscopies were used. The results indicated that gypsum is the main component of the stucco mask. As well, calcite was found as an impurity or an addition together with halite and anhydrite as deterioration products. The iron nail corrosion was the reason for a notable discoloration to the stucco mask. FTIR analysis confirms that animal glue is the adhesive material used for the reassembly of the mask. The used pigments for painting the mask are hematite for red-orange color, mixture of hematite and magnetite for brown color and mixture of gypsum and hematite for the white brown color.

A Study on the Materials Used in the Ancient Architectural Paintings from the Qing Dynasty Tibetan Buddhist Monastery of Puren, China.

Situated in the village of Lama Temple on the eastern bank of the Wulie River in Chengde, Puren Temple stands as one of the few remaining royal temples of great importance from the Kangxi era (1662-1722 AD). This ancient edifice has greatly contributed to the advancement of our comprehension regarding the art of royal temple painting. The present study undertakes a comprehensive analysis and identification of nine samples obtained from the beams and ceiling paintings within the main hall of Puren Temple. Furthermore, a systematic examination of their mineral pigments and adhesives is conducted. The findings from polarized light microscopy (PLM), energy-type X-ray fluorescence spectrometer (ED-XRF), micro-Raman spectroscopy (m-RS), and X-ray diffractometer (XRD) analyses reveal that the pigments present in the main hall beams of Puren Temple are cinnabar, lead white, lapis lazuli, and lime green, while the pigments in the ceiling paintings consist of cinnabar, staghorn, lead white, lapis lazuli, and lime green. The use of animal glue as a binder for these pigments on both the main hall beams and ceiling paintings is confirmed via pyrolysis-gas chromatography-mass spectrometry (Py-Gc/Ms) results. These findings hold significant implications for the future restoration of Puren Temple, as they provide valuable guidance for the selection of appropriate restoration materials.

Canine ex vivo tarsal arthrodesis: fixation by using a new bone tissue glue.

Arthrodesis, performed as a salvage surgical procedure to treat intractable joint conditions in dogs and cats, is associated with a high incidence of complications intra and postoperative, proving the need for improved and new techniques in arthrodesis surgery. Adding a new resorbable bone glue to the arthrodesis could potentially add fixation strength and lower complications. The objectives of this experimental ex vivo biomechanical study were therefore to develop a biomechanical test model of partial tarsal arthrodesis and to determine whether the new resorbable bone glue (phosphoserine modified cement) produced measurable fixation strength in canine calcaneoquartal arthrodesis, without orthopedic implants. Four biomechanical test models with a total of 35 canine tarsal joints were used. Soft tissues were dissected to 4 different test models with variable contributions from soft tissues. The calcaneoquartal joint was prepared as in vivo arthrodesis and the glue was applied to joint surfaces as a liquid/putty (0.4 cc). After curing for 24 h, a shear force was applied to the joint (1 mm per minute) and the failure strength was recorded. Calcaneoquartal joints, where all soft tissues had been completely resected and fixated with glue (1-1.5 cm2 joint surface), withstood 2-5 mm of displacement and an average of 100 ± 58 N/cm2 of shear force (Model 1). Similar adhesive fixation strengths were obtained in Model 2 and 3 with increasing contributions from soft tissues (80 ± 44 and 63 ± 23 N/cm2, p = 0.39, ANOVA). The developed biomechanical model was sensitive enough to measure differences in fixation strengths between different glue formulations. The average fixation strength (60-100 N/cm2) should be strong enough to support short-term load bearing in medium sized canines (20 kg). The developed cadaver biomechanical test model is of potential use for other arthrodesis studies. The new resorbable glue can potentially contribute to stability at arthrodesis surgery, acting as a complement to today's standard fixation, metal implants.