Bond Strength Of Self-etching Adhesives Research Articles

Evaluation of Casein Phosphopeptide–Amorphous Calcium Phosphate Effect on the Bond Strength of Self-etch Adhesives to Dentin: A Systematic Review

Evaluation of Casein Phosphopeptide–Amorphous Calcium Phosphate Effect on the Bond Strength of Self-etch Adhesives to Dentin: A Systematic Review

Delayed light activation of resin composite affects the bond strength of adhesives under dynamic simulated pulpal pressure.

To evaluate the effect of a time delay before the light activation of resin composite on the microtensile bond strength (µTBS) of self-etch adhesives under dynamic simulated pulpal pressure. One hundred twenty crown segments were prepared from human third molars. Dentin surfaces were prepared with coarse diamond burs and connected to a dynamic pulpal pressure device. The self-etch adhesives used were Clearfil SE Bond (CSE), G2-Bond Universal (G2B), Clearfil Universal Bond Quick (CBQ), and G-Premio Bond (GPB). Each adhesive was divided into 3 subgroups: immediate light activation of resin composite (T0), delayed light activation for 150s (T1), and 300s (T2). The µTBS data were obtained after 24-h water storage. Scanning electron microscopy was used to evaluate the nanoleakage at the resin-dentin surface. The µTBS data were analyzed using two-way ANOVA and Duncan's multiple comparisons. All adhesives demonstrated a significant reduction in µTBS at T2, except for CBQ where the bond strength was not affected by the delayed light activation times. The two-step self-etch adhesives (CSE and G2B) demonstrated higher bond strength than the one-step adhesives at all conditions. Nanoleakage was observed in all adhesives at T2; however, nanoleakage was detected only in CBQ and GPB at T1. Delayed light activation of resin composite adversely affected the dentin bond strength of self-etch adhesives. Two-step self-etch adhesives had better bonding performance than one-step self-etch adhesives. After adhesive application, resin composite should be adapted and cured as soon as possible.

Evaluation of the Bond Strength of Self-Etching Adhesive Systems Containing HEMA and 10-MDP Monomers: Bond Strength of Adhesives Containing HEMA and 10-MDP.

The aim of this in vitro study was to assess the bond strength of self-etching adhesives containing HEMA and 10-MDP monomers. Twenty-four bovine teeth were divided into three groups. Two cylinders of composite resin were made in each tooth (n = 16): G1-Prime and Bond Universal (control); G2-OptiBond All-in-One (HEMA); and G3-Clearfil SE (10-MDP and HEMA). After 24-hour storage in distilled water, the specimens were fixed to a universal testing machine (Kratos Equipamentos Ltda.) for the microshear test at a speed of 0.5 mm/min. A qualitative analysis of the fracture pattern was also performed using scanning electron microscopy (500× magnification). The normality of sample data distribution was determined using the Shapiro–Wilk test. The results were assessed using the Kruskal–Wallis test, and α level of 5% was used for the analysis. The results indicated a statistical difference (p > 0.05) between G3 (15.6080 MPa) and G2 (11.2180 MPa). No statistical difference was observed when G1 (14,6325 MPa) was compared with the other two groups. It was also observed that a mixed fracture pattern was predominant in all groups. The self-etching adhesive containing HEMA and 10-MDP monomers showed to be promising in increasing the bond strength between the dental substrate and the composite resin, whereas the adhesive containing only HEMA exhibited lower bond strength to dentin.

The influence of dimethyl sulfoxide on resin–dentin bonding: A systematic review

This article aims to systematically review published literature about the influence of dimethyl sulfoxide (DMSO) on resin–dentin bonding. Online databases (Medline (PubMed), Embase, Web of Science, and the Cochrane Library) were searched on this topic. Gray literature was searched using Google Scholar. Published works until December 2020 were collected, and pertinent articles were selected in accordance with the eligibility criteria. Quality assessment was conducted via the modified Consolidated Standards of Reporting Trials (CONSORT) checklist and the modified Cochrane risk of bias tool. Thirty-eight studies were identified, twelve of which satisfied the eligibility criteria were included in this review. Micro tensile bond strength (MTBS) was set as the main outcome. Secondary outcomes include nanoleakage evaluation, the effect of DMSO on dentin collagen fibrils, and protease inhibition. Several studies proved the promotion of DMSO on long-term dentin bond strength of etch-and-rinse adhesives. However, evidence about its effect on bond strength of self-etch adhesives was insufficient. Different concentrations of DMSO solution also cause diverse impact on dentin bonds. Based on the included studies, DMSO considerably affects resin–dentin bonds by dissociating demineralized dentin matrix, inhibiting matrix metalloproteinases (MMPs), and enhancing penetration of resin monomers. The influence of DMSO on dentin bonding may offer inspirations for dental clinical practice to optimize resin–dentin bonds. • DMSO is beneficial for improving the long-term dentin bond strength of etch-and-rinse adhesives. • The influence of DMSO on self-etch adhesives remains controversial and needs more validation. • DMSO can dissociate demineralized dentin matrix, inhibit matrix metalloproteinases, and enhance resin monomer penetration. • DMSO may enlighten the concept of “wet bonding” technique from surface moisture to bio-modification of dentin. • DMSO offers inspiration for dental clinical practice to optimize resin–dentin bonds.

Comparative evaluation of different surface treatments of the salivary contaminated dentin on the shear bond strength of self-etch adhesives: An in vitro study.

Aims and Objectives:This study aimed to evaluate and compare the effect of different surface treatments of the salivary contaminated dentin on the shear bond strength of one-step self-etch adhesives.Materials and Methods:The coronal buccal surfaces of 50 human mandibular molars were ground to expose dentin. The specimens were randomly divided into five groups. Ten teeth were left uncontaminated and served as control group. Rest of the teeth were then divided based on the decontamination method used as follows: Group II (no decontamination done), Group III (decontamination with rinsing), and Group IV (decontamination with etching), Group V (decontamination with ethanol). Composite resin cylinders of 4 mm diameter and 4 mm length were fabricated on the surfaces. Shear bond strength testing was done in a universal testing machine, and the data were subjected to a one-way analysis of variance and KruskalWallis test. Intergroup comparison was made using MannWhitney U-test.Results:The highest bond strength was seen in Group 1, followed by and least in Group II. The difference was found to be statistically significant among all the groups. Group II and Group IV showed lower shear bond strength than Group I (control) and Group III (P < 0.05). The intergroup comparison shows significant differences between Group I with all the other groups.Conclusion:It was concluded that the use of decontaminant improved the bond strength to salivary contaminated dentin in self-etch adhesives.

Deproteinization of caries-affected dentin with chemo-mechanical caries removal agents and its effect on dentin bonding with self-etch adhesives

ObjectivesTo evaluate the micro-tensile bond strength (μTBS) of self-etch adhesives (SEA) to normal and caries-affected dentin after smear layer deproteinization with papain enzyme-based and NaOCl-based chemo-mechanical caries removal agents (CRAs) and to compare their efficacy to a 6 % NaOCl solution. MethodsForty extracted human molars with occlusal carious lesions were randomly divided into four pretreatment groups: no treatment (control), Papacarie for 60 s, Carisolv for 60 s, and 6 % NaOCl for 15 s. After pretreatment, Universal Bond Quick ER (UBQ, one-step, 1-SEA) or Clearfil SE Bond 2 (CSE, two-step, 2-SEA) was applied to the pre-treated surface, and built-up with resin composite (Clearfil AP-X). The μTBS test was performed after 24 h water storage of the specimens at 37 °C, and the modes of failure were determined under SEM. Deproteinizing effects of the tested agents on smear layer-covered dentin was quantified by changes in the amide:phosphate ratio using attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) analysis. ResultsOn caries-affected dentin the μTBS improved significantly after pretreatment with Papacarie, Carisolv, and 6 % NaOCl with both SEAs (p < 0.05). Papacarie pretreatment significantly improved the μTBS of UBQ on normal dentin compared to NaOCl pretreatment (p < 0.05). ATR-FTIR revealed that all agents significantly decreased the amide:phosphate ratio on the smear layer-covered normal and caries-affected dentin (p < 0.05). ConclusionsSmear layer deproteinizing with papain enzyme-based and NaOCl-based gel CRAs was effective in improving the micro-tensile bond strength of self-etch adhesives to caries-affected dentin. Clinical significanceSmear layer deproteinization with Papacarie (a papain enzyme-based gel) or Carisolv (a NaOCl-based gel) can improve the μTBS to normal and caries-affected dentin bonded with 2-step self-etch adhesives.

The effect of silver diamine fluoride on bond strength of self-etch adhesives to demineralized dentin (An In-vitro study)

The effect of silver diamine fluoride on bond strength of self-etch adhesives to demineralized dentin (An In-vitro study)

Biomimetic fabrication and application of fibrous-like nanotubes

AimsTo investigate the biomimetic fabrication of fibrous-like organic-inorganic hybrid structures via a simple bottom-up approach, viz. self-assembly of simple molecules, and apply fibrous-like composites as a novel primer to improve dentin bond strengths of self-etch adhesives. Materials and methodsThe resultants of commercial amorphous calcium phosphate (ACP) nanoparticles and 10-methacryloyloxydecyl dihydrogen phosphate (MDP) ethanol-aqueous solution were analyzed by TEM, SEM, XRD, DLS and AFM. The acid and alkali resistance of abovementioned self-assembled composites were analyzed with TEM. Micro-tensile bond strengths (MTBS) tests were performed after polished dentin surfaces were pretreated with self-assembled composites. The pretreated dentin surfaces and dentin-resin interfaces were characterized by SEM/TEM. Key findingsACP nanoparticles in MDP solution could self-assemble into fibrous-like nanotube structures in 8 nm diameter. Self-assembly and self-proliferation process went from ACP nanoparticles, dissolved ACP nanoparticles (less than 50 nm), twig-like structures and fibrous-like nanotubes to cellular networks. The fibrous-like nanotubes were only detected when the amount of ACP in reaction system were more than 0.01 g. The more ACP interacted with MDP, the more fibrous-like nanotubes were formed. After the dentin surfaces were treated with fibrous-like nanotube composites, MTBS could be significantly improved. Moreover, the fibrous-like nanotube structures could be resistant to acidic challenge, and were stable at least for 3 months. SignificanceThe fibrous-like nanotube structures could be self-assembled via a bottom-up approach at certain ratio of MDP and commercial ACP nanoparticles. The application of fibrous-like nanotube composites as a novel primer prior to self-etch adhesives greatly improved dentin bond strengths.

Effects of remaining dentin thickness, smear layer and aging on the bond strengths of self-etch adhesives to dentin.

This study evaluated the effects of remaining dentin thickness (RDT), different smear layers, and aging on the microtensile bond strength (µTBS) of universal adhesives to dentin when applied in self-etch mode. Ninety-six human third molars were randomly allocated to 12 groups (n=8) based on adhesives: ClearfilTM SE Bond 2 (SE, control), ClearfilTM Universal Bond (CU) and ScotchBondTM Universal Adhesive (SB); smear layers: prepared either with 600-grit SiC paper (P) or regular diamond bur (B); and aging: stored in distilled water at 37ºC for 24 hours (24h) or 1 year (1y). µTBS was significantly affected by the type of adhesives, smear layers, and aging (p<0.001). A statistically significant and positive linear relationship was also observed between µTBS and RDT (p<0.05) in all the tested groups, except for SEB1y and CUB24h (p>0.05). RDT, smear layer types, and aging can influence the bonding performances of universal adhesives when applied in self-etch mode.

Effect of erosive challenge and cigarette smoke on dentin microhardness, surface morphology and bond strength

Objective: This study aimed to evaluate the surface microhardness and morphology, as well as the microshear bond strength of a self-etching adhesive (Clearfil SE, Kuraray) to eroded dentin, exposed or not to cigarette smoke. Forty dental crowns were divided into 4 groups (n = 10): no treatment (control) (C); erosion (E); erosion + cigarette smoke exposure (ES); cigarette smoke exposure (S). Samples were prepared through third molars polishing until dentin exposure, followed by crown section. Erosive cycles were performed 5 times/day for 30 s at 60 min intervals. Cigarette smoke was produced with twenty cigarettes/day, during 5 days. Microhardness was evaluated initially and after the treatments. Microshear bond strength was tested after the treatments and dentin restoration with flow composite. Failure patterns and dentin morphology was evaluated by Scanning Electron Microscopy. Microshear bond strength data was submitted to two-way ANOVA, microharness test was adjusted by gamma distribution to be a non-parametric analyses (p=0.05), and surface morphology as qualitative analyses. Loss percentage of microhardness was observed only in groups submitted to erosion. Bond strength was statistically similar between all groups. The most prevalent failure pattern was of adhesive type. Morphological analysis of dentin showed obliterated tubules in groups submitted to cigarette smoke exposure. Cigarette smoke exposure did not promote any effect in the percentage of microhardness loss, as in sound dentin as in eroded dentin. Cigarette smoke, erosion, and association of both, did not alter the bond strength of self-etching adhesives to dentin.KeywordsBond strength; Cigarette Smoke; Dental erosion; Microhardness; Self-etching adhesives.

Comparative evaluation of clinical performance of two self-etch adhesive systems with total-etch adhesive system in noncarious cervical lesions: An in vivo study.

Background:The growing demands for esthetic restorations have stimulated intensive research in the field of adhesive dentistry. Dental adhesive systems are used to promote adhesion between composite resins and dental structure. In the fundamental principles of adhesion, the primary mechanism contributing to the formation of adhesion is micromechanical bonding between the restoration and the tooth. The bond strength of self-etching adhesives to dentin was found to be almost equal to that of total-etch adhesives. The aim of the present prospective, double-blind, randomized controlled clinical trial was to evaluate and compare the clinical performance of two self-etch adhesive systems with total-etch adhesive system in noncarious cervical lesions (NCCLs).Materials and Methods:In each patient, three teeth were randomly assigned according to the adhesive system used to Group A (total-etch adhesive system), Group B (two-bottle self-etch adhesive system), and Group C (one-bottle self-etch adhesive system). The clinical efficacy of these adhesive systems was determined by evaluating the retention rate, marginal integrity, and postoperative sensitivity at the following three levels: baseline, 6 months, and 18 months by following the Modified USPHS criteria introduced by Vanherle et al.Results:In the present study, the retention rate at 18 month in Group A, Group B, and Group C of 96%, 92%, and 92% was observed, respectively. A marginal integrity at 18 months was 88%, 80%, and 84% for Group A, Group B, and Group C, respectively. Postoperative sensitivity at 18 months was 16%, 12%, and 12% for Group A, Group B, and Group C, respectively.Conclusion:The clinical performance of total-etch and self-etch adhesive systems in NCCLs did not differ significantly with regard to the evaluated parameters – retention, marginal integrity, and postoperative sensitivity.

Bond strength of self-etching adhesives to dentin surface after smear layer removal with ultrasonic brushing.

This study determined the micro-shear bond strength (µSBS) of two self-etching adhesives to dentin surface after smear layer removal with a novel ultrasonic brushing method. Ninety-five dentin discs were ground flat with diamond burs and divided into 5 groups; no treatment (control), brushing with a custom-made brush for 10 and 30 s without and with ultrasonic application. The smear layer thickness was evaluated using SEM. The 24 h µSBS of Clearfil SE Bond (CSE) and Clearfil Tri S Bond (CTS) to these dentin surfaces were evaluated. Smear layer thickness decreased significantly from control, brushing without, and brushing with ultrasonic groups, respectively. The µSBS of CSE were higher than CTS in all groups. The µSBS were improved with ultrasonic brushing for 30 s for both adhesives. Ultrasonic brushing is the effective method in mechanical smear layer removal which can improve bond strength of self-etching adhesives to ground dentin.

Prime-and-rinse approach for improving the enamel micro-tensile bond strengths of self-etch adhesives

The study investigated the effects of prime-and-rinse approach using 15% MDP (10-methacryloyloxydecyl dihydrogen phosphate)-containing primer on the enamel micro-tensile bond strengths (MTBS) of (ultra-) mild self-etch adhesives, enamel surfaces and enamel-resin interfaces. The buccal enamel surfaces of 69 human third molars were polished and randomly assigned to three groups: Group A (control, self-etch approach): Polished enamel surfaces were not further pre-treated. The enamel surfaces were acid-etched (Group B, (selective) enamel etching) or primed with 15% MDP-containing primer (Group C, prime-and-rinse approach) for 15 s and thoroughly water-sprayed. The enamel surfaces were applied with self-etch adhesives and placed with composite resins (Adper Easy One + Filtek Z350 (3 M ESPE); Clearfil S3 Bond + Clearfil Majesty (Kuraray-Noritake Co.); G Bond + Gradia Direct (GC); iBond + Charisma (Heraeus-Kulzer)), respectively. The specimens were prepared for MTBS test and scanning/transmission electron microscopy observations. Compared with group A, groups B and C produced significantly higher enamel MTBS (p < .01), regardless of the adhesives used. Groups B and C possessed similar enamel MTBS (p > .05). The SEM findings showed that smear layer remained on the polished enamel surface was completely removed by acid etching and almost completely removed by prime-and-rinse approach. The TEM microphotographs reveal that smear layer was detectable at the resin-enamel interface in group A, not in groups B and C. The novel prime-and-rinse approach using MDP-containing primer before the application of (ultra-) mild self-etch adhesives could greatly increase the enamel MTBS. That might be an alternative to selective enamel etching.

Effect of Metalloproteinase Inhibitors on Bond Strength of a Self-etching Adhesive on Erosively Demineralized Dentin.

To analyze the influence of epigallocatechin-3-gallate (EGCG) and chlorhexidine (CHX) on adhesive-dentin bond strength of a self-etch adhesive to sound dentin (SD) and eroded dentin (ED). Thirty-six middle-dentin samples were assigned to six groups (n = 6) according to pretreatment (DW: distilled water, control; 0.1% EGCG; or 2% CHX) and erosive challenge (presence or absence). Specimens were subjected to 2-h acquired pellicle formation, then half of them were exposed to 1% citric acid three times a day for five days. SD and ED were treated with the tested solutions for 60 s, and then Clearfil SE Bond was applied before resin composite buildup. Bonded teeth were longitudinally sectioned into sticks and half were immediately tested, while the remaining specimens were tested after six months. The mode of fracture was examined and the microtensile bond strength (µTBS) measured. Statistical analysis was performed with ANOVA and Bonferroni tests. At both time periods, regardless of the dentin substrate, EGCG groups did not show bond strengths that were significantly different from those obtained with DW (p > 0.05), while CHX generated lower values than did DW (p < 0.05). On SD, there was a bond strength reduction only in the CHX groups after six months. However, for ED, the bond strength significantly decreased in all groups. CHX negatively affected both dentin substrates, while the pretreatment with EGCG did not affect µTBS over time on SD. µTBS may be influenced by the substrate over time and EGCG can be used as an alternative to CHX to maintain the bond strength of self-etching adhesives.

The Influence of a Touch-cure System on the Micro-tensile Bond Strength of Self-etch Adhesives to Root Canal Dentin

The Influence of a Touch-cure System on the Micro-tensile Bond Strength of Self-etch Adhesives to Root Canal Dentin

Effect of smear layer and surface roughness on resin-dentin bond strength of self-etching adhesives.

The purpose of this study was to evaluate the effect of surface roughness and smear layer on the micro-shear bond strength (µSBS) of two self-etching adhesives, Clearfil SE Bond (SE) and Clearfil Tri-S Bond (S3). Flat dentin surfaces were prepared with SiC papers (600-, 180- and 120-grit) and diamond burs (extra fine, medium and coarse). They were further divided into smear-covered and smear-free surfaces and bonded with respective adhesives. µSBS test was performed after water storage at 37ºC for 24 h. Smear layer thickness measurement was evaluated by scanning electron microscope (SEM). Surface roughness was examined by contact stylus profilometer. Smear layer thickness and surface roughness were significantly different among all groups (p<0.05). No difference in µSBS was observed among surface prepared by SiC whereas bur-cut smear layer had negative effect on µSBS, especially for S3. Surface roughness from different surface preparations had no influence on µSBS (p<0.05).

Effect of acid erosion on enamel bond strength of self-etch adhesives and sonic velocity measurement of enamel.

The purpose of this study was to investigate the effect of acid erosion on the bonding performance of universal adhesives. Freshly extracted bovine teeth were cut into enamel slabs and assigned to either the Er or the control group. Specimens in the Er group were immersed in citric acid solution (pH 2.1) twice a day and stored in artificial saliva, while control specimens were simply stored in artificial saliva. Differences in the mean values between the control and Er groups were greater than expected, despite accounting for the effect of differences in the adhesive systems (p=0.016). The Er group mainly exhibited cohesive failure in the enamel, while adhesive failure was more frequent in the control group. The sonic velocity was found to decrease over time in the acid attacked specimens. The results indicated that bond strengths of universal adhesive tested increased in eroded enamel.

Microtensile bond strength of self-etching adhesives to enamel

Purpose It is uncertain whether single-step self-etching adhesives form bond to enamel as reliable as those of etch-and-rinse adhesives. This study compared the microtensile bond strengths to ground enamel of three one-step self-etching adhesive systems, a self-etching primer system and an etch-and-rinse adhesive system. Materials and methods Human enamel was ground flat with 320-grit silicon carbide paper. The self-etching adhesives Clearfil Tri S Bond (Kuraray), Futurabond NR (Voco) and Hybrid Bond (Sun-Medical), the adhesive with a self-etching primer Clearfil SE Bond (Kuraray) and the etch-and-rinse adhesive Admira Bond (Voco) were applied as directed, followed by a core of the same manufacturers' hybrid resin composite. A microtensile bond strength evaluation was performed after 48 h of water storage, using untrimmed beams ~1.0 ± 0.1 mm2 in cross-sectional area at a cross-head speed of 0.5 mm/min. Results There were no pretest failures in any group, and failures were predominately adhesive or mixed. Adhesion to enamel of Futurabond NR and Clearfil SE Bond was not significantly different from Admira Bond, while Clearfil Tri S Bond and Hybrid Bond demonstrated significantly lower bond strengths (one-way analysis of variance, Tukey–Kramer multiple-comparison test, P Clinical significance Bond strength to ground enamel of self-etching adhesive systems was dependant on the type of adhesive and some materials showed bond strength that was not different than that of etch-and-rinse system.

The effect of Er:YAG laser irradiation on the bond stability of self-etch adhesives at different dentin depths

The aim of this study was to evaluate the effect of Er:YAG laser irradiation on the micro-shear bond strength of self-etch adhesives to the superficial dentin and the deep dentin before and after thermocycling. Superficial dentin and deep dentin surfaces were prepared by flattening of the occlusal surfaces of extracted human third molars. The deep or superficial dentin specimens were randomized into three groups according to the following surface treatments: group I (control group), group II (Er:YAG laser; 1.2 W), and group III (Er:YAG laser; 0.5 W). Clearfil SE Bond or Clearfil S3 Bond was applied to each group’s dentin surfaces. After construction of the composite blocks on the dentin surface, the micro-shear bond testing of each adhesive was performed at 24 h or after 15,000 thermal cycles. The data were analyzed using a univariate analysis of variance and Tukey’s test (p < 0.05). Laser irradiation in superficial dentin did not significantly affect bond strength after thermocycling (p > 0.05). However, deep-dentin specimens irradiated with laser showed significantly higher bond strengths than did control specimens after thermocycling (p < 0.05). Thermocycling led to significant deterioration in the bond strengths of all deep-dentin groups. The stable bond strength after thermocycling was measured for all of the superficial-dentin groups. No significant difference was found between the 0.5 and 1.2 W output power settings. In conclusion, the effect of laser irradiation on the bond strength of self-etch adhesives may be altered by the dentin depth. Regardless of the applied surface treatment, deep dentin showed significant bond degradation.

Bond Strength Comparison of One-Step/Two-Step Self-Etch Adhesives to Cavity Floor Dentin after 2.5 Years Storage in Water

In recent years, decrease in dentin bond strength of one-step self-etch adhesives after long-term water storage has been reported; however, the flat dentin surface of the extracted tooth has been used to carry out the bond strength tests in the majority of the previous studies. Objectives: The purpose of this study was to compare the micro-tensile-bond-strength (μTBS) of three one-step self-etch adhesives and a two-step self-etch adhesive on the cavity floor dentin after 2.5 years storage in distilled water. Materials and Methods: Adhesives were used in this study: three one-step self-etch adhesives (Primefil, GBOND PLUS and Beauti Bond), and one two-step self-etch adhesive (Clearfil SE Bond), which was used as the control. Bowl-shaped cavities of extracted human incisors were treated with each adhesive and filled with each flowable resin composite from the same manufacturer. The specimens were stored in distilled water at room temperature for two and a half years. The beam samples were made and subjected to μTBS test using the tabletopmaterial- tester. The data were statistically analyzed using one-way ANOVA. Results: The μTBS value of G-BOND PLUS was higher than that of other adhesives; however, the results of ANOVA showed no significant differences in μTBS values among all adhesives. Conclusion: There was no significant difference between one-step and two-step on the bond strength of selfetch adhesives to the cavity dentin floor after a storage period of 2.5 year.