Bona Fide Research Articles

“Bona Fide” Merchants: Negotiating Life, Labor, and Transnational Mobility in the Time of Chinese Exclusion

From 1875 until 1943, various policies increasingly circumscribed the free movement of Chinese immigrants into and within the United States. These efforts had a profound and lasting impact on the Chinese diaspora in the Pacific Northwest and divided the Chinese community into two distinct classes: laborers and a privileged class that included merchants. The authors of this research article argue that merchant businesses served a critical and multifaceted role in the formation, development, and decline of rural Chinatowns and in the lives of Chinese Oregonians who used the businesses to facilitate resistance and community persistence. Attaining officially recognized merchant status offered certainty and stability, as well as social and mobility, providing immigrants and their families with opportunities for prosperity in a largely unwelcoming land. The Wing Hong Hai Company Store (永同泰) in The Dalles (姐里阜) and the Wah Chung and Company Store (和昌) in Ashland each played important roles in the maintenance of Oregon’s Chinese diaspora communities. On the surface, they bought and sold goods and services; in reality, these transnational establishments did so much more as they navigated the waters of Chinese exclusion.

“Bona Fide” Merchants: Negotiating Life, Labor, and Transnational Mobility in the Time of Chinese Exclusion

“Bona Fide” Merchants: Negotiating Life, Labor, and Transnational Mobility in the Time of Chinese Exclusion

Supplemental Material for Detecting Simulated Versus Bona Fide Traumatic Brain Injury Using Pupillometry

Supplemental Material for Detecting Simulated Versus Bona Fide Traumatic Brain Injury Using Pupillometry

Plasmin-Induced Activation of Human Platelets Is Modulated by Thrombospondin-1, Bona Fide Misfolded Proteins and Thiol Isomerases.

Inflammatory processes are triggered by the fibrinolytic enzyme plasmin. Tissue-type plasminogen activator, which cleaves plasminogen to plasmin, can be activated by the cross-β-structure of misfolded proteins. Misfolded protein aggregates also represent substrates for plasmin, promoting their degradation, and are potent platelet agonists. However, the regulation of plasmin-mediated platelet activation by misfolded proteins and vice versa is incompletely understood. In this study, we hypothesize that plasmin acts as potent agonist of human platelets in vitro after short-term incubation at room temperature, and that the response to thrombospondin-1 and the bona fide misfolded proteins Eap and SCN−-denatured IgG interfere with plasmin, thereby modulating platelet activation. Plasmin dose-dependently induced CD62P surface expression on, and binding of fibrinogen to, human platelets in the absence/presence of plasma and in citrated whole blood, as analyzed by flow cytometry. Thrombospondin-1 pre-incubated with plasmin enhanced these plasmin-induced platelet responses at low concentration and diminished them at higher dose. Platelet fibrinogen binding was dose-dependently induced by the C-terminal thrombospondin-1 peptide RFYVVMWK, Eap or NaSCN-treated IgG, but diminished in the presence of plasmin. Blocking enzymatically catalyzed thiol-isomerization decreased plasmin-induced platelet responses, suggesting that plasmin activates platelets in a thiol-dependent manner. Thrombospondin-1, depending on the concentration, may act as cofactor or inhibitor of plasmin-induced platelet activation, and plasmin blocks platelet activation induced by misfolded proteins and vice versa, which might be of clinical relevance.

Leveraging Zebrafish To Study Bona Fide Astrocytes

In a recent study, Chen and colleagues demonstrated that zebrafish spinal cord radial glia differentiate into cells that are similar to mammalian astrocytes. This study highlights the validity of the zebrafish model for discovering molecular mechanisms governing astrocyte function.

Acute Kidney Injury: A Bona Fide Complication of Diabetes.

The landscape of kidney disease in diabetes has shifted. The classical dogma of "diabetic nephropathy" progressing through stages of albuminuria, leading to decline in glomerular filtration rate and end-stage kidney disease (ESKD), has been replaced by a more nuanced understanding of the complex and heterogeneous nature of kidney disease in diabetes. Paralleling this evolution, standardized definitions have resulted in a growing appreciation that acute kidney injury (AKI) is increasing in its incidence rapidly and that people with diabetes are much more likely to develop AKI than people without diabetes. Here, I propose that AKI should be considered a complication of diabetes alongside other complications that similarly do not fit neatly into the historical microvascular/macrovascular paradigm. In this article, we take a look at the evidence indicating that diabetes is a major risk factor for AKI and we review the causes of this increased risk. We consider the long-term implications of AKI in diabetes and its potential contribution to the future development of chronic kidney disease, ESKD, and mortality. Finally, we look toward the future at strategies to better identify people at risk for AKI and to develop new approaches to improve AKI outcomes. Recognizing AKI as a bona fide complication of diabetes should open up new avenues for investigation that may ultimately improve the outlook for people living with diabetes and at risk for kidney disease.

Compensation to the Bona Fide Homeowner for the Loss of Residential Premises2

New rules for compensation to the bona fide houseowner (physical entity) for the loss of residential premises were adopted were adopted on 1 January 2020. Such compensation is not tortious in nature. It is comparable to the real damage or cadastral value of a vindicated apartment. The amount of compensation is now not limited to 1 million rubles, and the period of impossibility of enforcement proceedings against perpetrators has been reduced to 6 months. At the same time, the date of entry into legal force of the court decision concerning the residential premises vindication and the date of the claim of a bona fide citizen for compensation in relation to 1 January 2020 affect the conditions of compensation, the amount of payment, the status of the respondent and the amount of budgetary funds. Taking into account the standing of the Constitutional Court of the Russian Federation in A. N. Dubovets case, it would be fair to assign risks of imperfection of the Unified State Register of Immovable Property (EGRN) to a public legal establishment and provide individuals with absolute protection from public vindication or, at least, envisage the possibility of compensation immediately after vindication without additional court proceedings. However, from the point of view of the economic efficiency of law, the law-maker expressed a preference for a legal model of protection of the original owner in order to discourage commission of offenses in the field of civil turnover of real property. The development of a fair system of compensatory measures for property vindication becomes a general trend in the legal mechanism of protection of civil rights in Russia.

Discrimination and the Bona Fide Occupational Qualification

There is a conversation going on today about race that has drawn attention to the inequalities and discrimination that many people did not see or were unwilling to see. The question explored today is if the systemic racism demonstrated on the streets mirrors the US workplace. A national survey conducted by the Pew Research Center found that blacks are more likely than whites to say black people are treated less fairly in the workplace (a difference of 42 percentage points). 1 On views of race and inequality, blacks and whites are worlds apart. Available at:https://www.pewsocialtrends.org/2016/06/27/on-views-of-race-and-inequality-blacks-and-whites-are-worlds-apart/. Accessed July 13, 2020. Google Scholar John R. Clark, JD, MBA, NRP, FP-C, CCP-C, CMTE, is the chief operating officer of the International Board of Specialty Certification and legal advisor for the International Association of Flight and Critical Care Paramedics.

MicroRNAs Regulated by the LPS/TLR2 Immune Axis as Bona Fide Biomarkers for Diagnosis of Acute Leptospirosis.

Leptospirosis remains a significant human health issue due to its systemic complications. Therefore, biomarkers that are more effective are urgently needed for the early diagnosis of leptospirosis. MicroRNAs (miRNAs) are evolutionarily conserved regulatory RNAs that have shown the potential to be used as biomarkers for diagnosis, prognosis, and therapy of infectious diseases. In this study, we performed an unbiased screen using the miRNome miRNA array to identify circulating miRNAs with the potential to serve as authentic biomarkers for early diagnosis of leptospirosis. Because leptospiral lipopolysaccharide (LPS) is the predominant leptospiral antigen and plays a vital role in immunological and biological activities, we used LPS treated and untreated in vitro (THP1 cells) and in vivo (BALB/c mice) surrogate models to identify the LPS-specific miRNAs. Differential expression analysis revealed 18 miRNAs to be associated strongly with LPS stimulation in THP1 cells. Of these, three (miR-let-7b-5p, miR-144-3p, and miR-21-5p) were observed to be present at increased levels in vivo The identified miRNAs were validated for their biomarker potential using serum samples from leptospirosis-negative patients and patients with confirmed cases of leptospirosis. Identified miRNAs were able to discriminate the acute leptospiral infection from other febrile diseases with a test sensitivity and specificity of 93.2% and 88.19%, respectively. Gene functional enrichment and protein-protein interaction (PPI) network analysis revealed that the identified miRNAs play important roles in disease signal transduction, signaling by interleukins, the stress-activated protein kinase signaling cascade, the mitogen-activated protein kinase (MAPK) signaling pathway, and the cellular response to a transforming growth factor β (TGF-β) stimulus with a notable interconnection between these biological processes.IMPORTANCE Here, we used miRNAs that are differentially regulated by the LPS/TLR2 immune axis to devise a miRNA-based diagnosis for leptospirosis. The study established the role of the circulating stable miRNAs (miR-21-5p, miR-144-3p, and miR-let-7b-5p) as an early diagnostic marker for leptospirosis. These miRNAs can be used to diagnose acute leptospirosis and also to differentiate leptospiral infection from other bacterial and spirochetal infections, as proved by the use of human clinical samples. Thus, our findings indicate that miRNAs can play a crucial role in the diagnosis of infectious diseases, like leptospirosis, that are generally misdiagnosed.

Legal Provisions Regarding Conversion Of Agricultural Land To Non-Agricultural Land

Agriculture Land is to be used for Non-agriculture purpose – Anything can work for the betterment of agriculture; this does not require the approval of the Collector – If a residential house is built on agricultural land but no farming is done, that house cannot be considered as a farmhouse – No Objection Certificate – it is not necessary to get NA permission for personal residence or agricultural development in rural areas – “Bona Fide industrial purpose” – The third party has no right to object to the non-cultivation permission procedure – “Tatkal Binkheti Yojana” (Immediate Non-agriculture Scheme) – Procedure – Checklist – Conditions to be fulfill for obtaining Non-agriculture permission – Landmark Judgments.

친권자에 의한 배임적 대리행위와 제3자 보호에 관한 판례 재검토 - 대법원 2018. 4. 26. 선고 2016다3201 판결 -

친권자에 의한 배임적 대리행위와 제3자 보호에 관한 판례 재검토 - 대법원 2018. 4. 26. 선고 2016다3201 판결 -

Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of Proteins.

The purification of plant cell walls is challenging because they constitute an open compartment which is not limited by a membrane like the cell organelles. Different strategies have been established to limit the contamination by proteins of other compartments in cell wall proteomics studies. Non-destructive methods rely on washing intact cells with various types of solutions without disrupting the plasma membrane in order to elute cell wall proteins. In contrast, destructive protocols involve the purification of cell walls prior to the extraction of proteins with salt solutions. In both cases, proteins known to be intracellular have been identified by mass spectrometry in cell wall proteomes. The aim of this chapter is to provide tools to assess the subcellular localization of the proteins identified in cell wall proteomics studies, including: (1) bioinformatic predictions, (2) immunocytolocalization of proteins of interest on tissue sections and (3) in muro observation of proteins of interest fused to reporter fluorescent proteins by confocal microscopy. Finally, a qualitative assessment of the work can be performed and the strategy used to prepare the samples can be optimized if necessary.

MnoSR Is a Bona Fide Two-Component System Involved in Methylotrophic Metabolism in Mycobacterium smegmatis.

Mycobacterium smegmatis and several other mycobacteria are able to utilize methanol as the sole source of carbon and energy. We recently showed that N,N-dimethyl-p-nitrosoaniline (NDMA)-dependent methanol dehydrogenase (Mno) is essential for the growth of M. smegmatis on methanol. Although Mno from this bacterium shares high homology with other known methanol dehydrogenases, methanol metabolism in M. smegmatis differs significantly from that of other described methylotrophs. In this study, we dissect the regulatory mechanism involved in the methylotrophic metabolism in M. smegmatis We identify a two-component system (TCS), mnoSR, that is involved in the regulation of mno expression. We show that the MnoSR TCS is comprised of a sensor kinase (MnoS) and a response regulator (MnoR). Our results demonstrate that MnoS undergoes autophosphorylation and is able to transfer its phosphate to MnoR by means of phosphotransferase activity. Furthermore, MnoR shows specific binding to the putative mno promoter region in vitro, thus suggesting its role in the regulation of mno expression. Additionally, we find that the MnoSR system is involved in the regulation of MSMEG_6239, which codes for a putative 1,3-propanediol dehydrogenase. We further show that M. smegmatis lacking mnoSR is unable to utilize methanol and 1,3-propanediol as the sole carbon source, which confirms the role of MnoSR in the regulation of alcohol metabolism. Our data, thus, suggest that the regulation of mno expression in M. smegmatis provides new insight into the regulation of methanol metabolism, which furthers our understanding of methylotrophy in mycobacteria.IMPORTANCE Methylotrophic metabolism has gained huge attention considering its broad application in ecology, agriculture, industries, and human health. The genus Mycobacterium comprises both pathogenic and nonpathogenic species. Several members of this genus are known to utilize methanol as the sole carbon source for growth. Although various pathways underlying methanol utilization have been established, the regulation of methylotrophic metabolism is not well studied. In the present work, we explore the regulation of methanol metabolism in M. smegmatis and discover a dedicated two-component system (TCS), MnoSR, that is involved in its regulation. We show that the loss of MnoSR renders the bacterium incapable of utilizing methanol and 1,3-propanediol as the sole carbon sources. Additionally, we establish that MnoS acts as the common sensor for the alcohols in M. smegmatis.

Exit Strategies in Interorganizational Collaboration: Setting the Stage for Re-Entry

Researchers have studied organizational exit primarily as a terminal process in which people engage in communication to manage and make sense of permanent closures of their relationships and membership. However, in interorganizational collaborations (IOCs), where individuals representing multiple organizations voluntarily come together and create a temporary alliance to address a shared problem, communication during the exit process can shape the collaborative partners’ post-exit relationships and future collaboration opportunities. To explore how such processes occur in the complex and dynamic environment of IOCs, this article develops a negotiated open process view of exit based on the Bona Fide Group Collaboration Model and presents a field study of IOCs in a regional planning context. Findings reveal three main strategies by which collaborative partners negotiated the open process of exit to secure their involvement in a future IOC. This article concludes with a discussion of implications for theorizing IOCs’ exit as part of a trajectory of ongoing collaborative relationships enacted by strategic communicative actions of collaborators.

美 · 日 경찰 체력검사 제도의 고찰 및 시사점

Starting from 2019, the system will be first implemented for male and female applicants for the National Police University and the National Police Agency, and it is expected that a combined selection of new police officers will also take place. Therefore, the study wanted to see the status of the U.S. police system, which applies the criteria for gender-unified physical fitness tests in most states, and the Japanese police`s physical fitness test system, which limits the number of female and female police officers, and dual standards for physical fitness tests. Unlike Korea and Japan, which restricts the number of women who hire police officers, in the United States, no policy has been implemented since the 1960s and 1970s to limit the number of female employees. Since it is very difficult to prove whether a Bona Fide Occurational Qualification (BFOQ) in the performance of police duties, the government has abolished the gender limit policy. If the number of female police officers is not limited by the abolition of gender separation, it is important to see whether the standard of employment, especially the standard of physical fitness test, will be equal. The claim that the standard of one-sided physical fitness test will adversely affect women`s acceptance rate has been used as a basis for the recruitment of gender separation. The United Kingdom and Australia, including more than 70 percent of the U.S. National Police Agency, apply the fitness test standards independently of gender. If the government abolishes the policy on limiting the number of female employees, it will apply the one-size-fits-all standard because controversy over reverse discrimination in the physical fitness test standards is growing. JAPPA, a Japanese police official`s physical fitness test system, does not have a standard for each age group of police officers and applies the same standard for all male and female police officers.

Computer-aided osteoporosis detection from DXA imaging

Background and objectiveOsteoporosis is a skeletal disease caused by a high rate of bone tissue loss, and it is a major cause of bone fracture. In contemporary society, osteoporosis is more common than cancer and stroke and results in a higher rate of morbidity and mortality in the human population. Osteoporosis can conclusively be diagnosed with dual energy X-ray absorptiometry (DXA). In this study, we propose a computer-aided osteoporosis detection (CAOD) technique that automatically measures bone mineral density (BMD) and generates an osteoporosis report from a DXA scan. MethodsThe CAOD model denoise and segments DXA images using a non-local mean filter, Machine learning pixel label random forest respectively, and locates regions of interest with higher accuracy. Pixel label random forest classifies a pixel either bone or soft tissue; then contours are extracted from binary image to locate regions of interest and calculate BMD from bone and soft tissues pixels. Mean standard deviation and correlation coefficients statistical analysis were used to evaluate the consistency and accuracy of BMD measurements. ResultsDuring a consistency test of BMD measurements using three consecutive scans from Computerized Imaging Reference Systems’ Bona Fide Phantom (CIRS-BFP) for the spine, the CAOD model showed an averaged standard deviation of 0.0029 while the standard deviation from manual measurements on the same data set by three different individuals was recorded as 0.1199. During another correlation study of BMD measurements evaluating real human scan images by the CAOD model versus manual measurement, the model scored a correlation coefficient of R2 = 0.9901 while the CIRS-BFP study scored a correlation coefficient of R2 = 0.9709. ConclusionsThe CAOD model increases the preciseness and accuracy of BMD measurements. This CAOD method will help clinicians, untrained DXA operators, and researchers (medical scientists, doctors, and bone researchers) use the DXA system with reliable accuracy and overcome workload challenges. It will also improve osteoporosis diagnosis from DXA systems and increase system performance and value.

Discovery and Validation of a PCR-Based mRNA Signature As a Bona Fide Prognostic Biomarker with Tumor-Intrinsic Properties in Stage II/III Colorectal Cancer and Its Application Irrespective of Tumor Subtypes

Discovery and Validation of a PCR-Based mRNA Signature As a Bona Fide Prognostic Biomarker with Tumor-Intrinsic Properties in Stage II/III Colorectal Cancer and Its Application Irrespective of Tumor Subtypes

Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-Derived Macrophages in Retinal Disease.

With the new understanding that adult microglia in mice have embryonic origins and are maintained in situ throughout life, it has become pertinent to now understand how these unique cells differ from monocyte-derived macrophages. The latter are recruited into the neural retina (and elsewhere in CNS) in certain diseased states, such as in various forms of retinal degeneration. However, phenotypic markers expressed by microglia and monocyte-derived macrophages largely overlap, thereby making it technically challenging to distinguish the two cell types in disease. To address this problem in mice, we have established an in vivo fate mapping system that enables distinguishing these two cell types in retinal disease models. Our approach leverages the seminal work that originally developed Cx3cr1-CreER mice and is based on commercially available mouse strains. Here, we detail our protocol and how to apply this fate mapping method paired with flow cytometry (or immunohistochemistry) to faithfully distinguish and examine microglia vs. monocyte-derived macrophages in a mutually exclusive manner. This approach will henceforth empower new efforts to identify functional specializations of these two populations in the pathobiology of retinal degenerative diseases and possibly other conditions of the retina where monocyte recruitment is observed, such as in glaucoma, diabetic retinopathy, ischemia reperfusion, retinal detachment, and so on.

Corrigendum Referring to: Getting the Message? Native Reactive Electrophiles Pass Two Out of Three Thresholds to be Bona Fide Signaling Mediators.

Corrigendum Referring to: Getting the Message? Native Reactive Electrophiles Pass Two Out of Three Thresholds to be Bona Fide Signaling Mediators.

A Population of Bona Fide Intermediate-mass Black Holes Identified as Low-luminosity Active Galactic Nuclei

Abstract Nearly every massive galaxy harbors a supermassive black hole (SMBH) in its nucleus. SMBH masses are millions to billions of solar mass, and they correlate with properties of spheroids of their host galaxies. While the SMBH growth channels, mergers, and gas accretion are well established, their origin remains uncertain: they could have emerged either from massive “seeds” (105–106 M ⊙) formed by direct collapse of gas clouds in the early universe or from smaller (100 M ⊙) BHs, end products of first stars. The latter channel would leave behind numerous intermediate-mass BHs (IMBHs, 102–105 M ⊙). Although many IMBH candidates have been identified, none are accepted as definitive; thus, their very existence is still debated. Using data mining in wide-field sky surveys and applying dedicated analysis to archival and follow-up optical spectra, we identified a sample of 305 IMBH candidates having masses 3 × 10 4 M ⊙ < M BH < 2 × 10 5 M ⊙ , which reside in galaxy centers and are accreting gas that creates characteristic signatures of a type I active galactic nucleus (AGN). We confirmed the AGN nature of 10 sources (including five previously known objects that validate our method) by detecting the X-ray emission from their accretion disks, thus defining the first bona fide sample of IMBHs in galactic nuclei. All IMBH host galaxies possess small bulges and sit on the low-mass extension of the M BH – M bulge scaling relation, suggesting that they must have experienced very few if any major mergers over their lifetime. The very existence of nuclear IMBHs supports the stellar-mass seed scenario of the massive BH formation.