Timber connections were prepared using glulam from tropical plantation species, focusing on key properties for dowel-type joints with half threaded bolts without nuts: Bolt bearing strength and bolt withdrawal capacity. Tests were performed according to ASTM standards. Three half-threaded bolt diameters (12 mm, 16 mm, and 20 mm) were tested in two loading directions, parallel and perpendicular to the grain, with 12 replicates for each configuration. Response Surface Methodology (RSM) using Design Expert Software was applied to optimize bolt diameter for both loading directions. Results showed that bolt bearing strength was higher in perpendicular loading, with the 12 mm bolt achieving 16.6 N/mm², compared to 6.01 N/mm² in parallel loading. Withdrawal capacities varied, with the 16 mm bolt showing the highest capacity in perpendicular loading at 54.2 kN. The study demonstrates that the 16 mm bolt exhibited the optimal diameter-to-embedment length ratio compared to 12 mm and 20 mm bolts, resulting in the highest withdrawal capacity. Consequently, the 16 mm bolt represented the best balance for achieving maximum withdrawal capacity. The optimization suggests using a 16 mm bolt for parallel loading to the grain and a 14 mm bolt for perpendicular loading.