This article presents an experimental investigation on the subcooled flow boiling heat transfer characteristics of CO2 in a horizontal tube with inner diameter of 6.16 mm below −30°C. The effects of mass and heat fluxes and saturation temperature on the heat transfer coefficient are discussed. Large deviations are noted between the predictions from previous empirical correlations and the current CO2 experimental data. Hence a new empirical correlation is developed, which agrees within ±30% with the current CO2 experimental data. It is expected that the data presented in this study would be beneficial to industry and designers of compact heat exchangers for CO2 at low temperatures.