Boer Buck Research Articles

Adding chitosan nanoparticles of green tea extract in diluent and thawing temperatures ameliorate the post-thawed quality of Boer buck semen

Objective: To improve the quality of post-thawing Boer buck semen for artificial insemination by adding green tea extract chitosan nanoparticles to skimmed egg yolk diluent, and the proper thawing temperature. Methods: The ejaculate of Boer buck was added to skimmed egg yolk diluent without (the control group) and with adding 1 μg of chitosan nanoparticles of green tea extract per mL of diluent (the treatment group). Then, the diluted semen was filled in French mini straws containing 60× 106 live sperm per straw, frozen in a standard protocol, and stored as frozen semen at -196 °C for a week. Six replicates from each group were diluted for 30 s at 37 °C or 39 °C sterile water to evaluate the semen quality. Results: Post-thawing (at 37 °C or 39 °C) of live sperm, progressive motility, and plasma membrane integrity were lower compared to those of the pre-freezing stage (P<0.05). Thawing at 37 °C resulted in no significant difference in live sperm, progressive motility, and plasma membrane between the control group and the treatment group (P>0.05). The live sperm, progressive motility, and plasma membrane of the treatment group in the pre-freezing stage, and post-thawed at 39 C were higher compared to those of the control group (P<0.05). There was no significant difference in malondialdehyde (MDA) concentration, DNA fragmentation, and catalase concentration of thawing at 37 °C compared to those of 39 °C in the same group. The MDA concentration and DNA fragmentation in thawing at 37 °C and 39 °C of the treatment group were significantly lower than those of the control group (P<0.05). However, the catalase concentration in thawing at 37 °C and 39 °C of the treatment group was not significantly different than the control group (P>0.05). Conclusions: Higher quality post-thawing Boer buck semen is achieved by adding 1 μg/mL of chitosan nanoparticles of green tea extract to the skimmed egg yolk diluent and thawing at 39 °C.

Effect of the addition of platelet-rich plasma to Boer buck semen on sperm quality and antioxidant activity before and after cryopreservation and in vivo fertility

The current study aimed to investigate the effects of incorporating platelet-rich plasma (PRP) into Boer buck semen at different stages of cryopreservation (dilution, equilibration, and post-thawing) on sperm quality, functionality, antioxidant activity, and in vivo fertility. One hundred sixty ejaculates were collected from eight Boer goat bucks over ten weeks during the breeding season. The semen samples were collected using an artificial vagina, evaluated, and pooled. The pooled semen was divided into four aliquots, then diluted with a Tris-based extender supplemented with varying proportions of PRP (0%, 5%, 10%, and 15%). Subsequently, the samples were equilibrated and cryopreserved. The results showed that semen supplemented with 10% PRP has significantly higher total motility (P < 0.05) compared to the control and other groups during (dilution and equilibration) and after (post-thawing) cryopreservation. Furthermore, semen supplemented with 5% and 10% PRP significantly improved (P < 0.05) rapid sperm movement compared to the control and 15% PRP groups after equilibration and post-thawing. However, compared to the control, the supplementation of 10% PRP resulted in improved sperm progressive motility and viability during and after cryopreservation (P < 0.05), as well as increased acrosome, plasma membrane integrity, and mitochondrial membrane potential after equilibration and post-thawing of the semen (P < 0.05). Moreover, 10% PRP-supplemented semen exhibited higher levels of glutathione peroxidase (GPx) and catalase (CAT) and lower levels of malondialdehyde (MDA) after equilibration and post-thawing compared to the control and 15% PRP groups. The pregnancy and kidding rates were higher (80%; 80%) for 10% PRP-supplemented semen compared to the control (48%; 40%), respectively, and other groups. In conclusion, the addition of PRP improved the cryo-tolerance of Boer buck sperm by enhancing quality, functionality, antioxidant activity, and in vivo fertility.

Novel Polymorphisms in Caprine Myostatin Gene and its Relationship with Growth Traits in Saburai Does (Capra hircus).

&lt;b&gt;Background and Objective:&lt;/b&gt; Saburai goat (&lt;i&gt;Capra hircus&lt;/i&gt;) is a crossbred goat from Boer buck (75%) and Ettawa doe (25%) for meat production purposes. This study was carried out to detect the mutation points or Single Nucleotide Polymorphisms (SNPs) in the myostatin (&lt;i&gt;MSTN&lt;/i&gt;) gene of Saburai with the forward sequencing method. &lt;b&gt;Materials and Methods:&lt;/b&gt; A total of twenty-one blood samples of Saburai does (75% Boer, 25% Ettawa) were collected from the Villager Breeder Center (VBC) at Tanggamus Regency of Lampung Province, Indonesia. The DNA analysis consists of DNA isolation, PCR analysis and sequencing analysis. The record data were used for association study with the mathematical model: Y&lt;sub&gt;ij&lt;/sub&gt; = μ+G&lt;sub&gt;i&lt;/sub&gt;+Є&lt;sub&gt;ij&lt;/sub&gt;. &lt;b&gt;Results:&lt;/b&gt; Research showed that one common SNP of g.217_218.indel.TTTTA (5'UTR) and three novel SNPs of c.386G>C (exon 1), g.641_642.indel.T (intron 2) and c.4957G>C (exon 3) were detected in the present study. In this study, a novel SNP on exon 1 and intron 2 of Saburai &lt;i&gt;MSTN&lt;/i&gt; gene has a moderate PIC value (>0.30). In addition, a novel SNP on exon 1 and exon 3 of the Saburai &lt;i&gt;MSTN&lt;/i&gt; gene was detected as a missense mutation of A55P and A43P, respectively. Goats with the heterozygous genotype have higher growth traits compared to goats with the homozygous genotype. &lt;b&gt;Conclusion:&lt;/b&gt; The goats with heterozygous genotypes can be further developed to increase the productivity of Saburai goats.

BOER BUCK SPERM QUALITY IN EXTENDER SUPPLEMETED WITH NON-ENZYMATIC ANTIOXIDANTS AND STORAGE AT COLD TEMPERATURE

The aim of this study was to determine the durability of standard extender tris egg yolk supplemented with non-enzymatic antioxidants from guava and fig fruit filtrate on the motility and viability of Boer buck sperm. Semen was collected using an artificial vagina every four days for ten replications. The treatment extenders were Tris+2.5% egg yolk (P0) and P0+1.487 mg vitamin A and P0+3.958 mg vitamin C per ml/100 ml (v/v) hereinafter referred as P1 and P2, respectively. The experimental design was a completely randomized 3 times 10 a factorial pattern. The variables assessed were individual motility, progessive motility and viability of sperm evaluated every day for 4 consecutive days. Result indicated that, 4 days after extended and stored at 5°C, the average individual motility, progessive motility and sperm viability were 45.2±7.94; 33.0±10.2 and 49.6±7.69 for P1, and 45.4±6.14; 30.4±8.38 and 50.5±5.88 for P2. The data on the three indicators of sperm quality between P1 and P2 did not show a significant difference (P0.05). While for P0 30.6±5.85; 24.6±7.30 and 40.7±5.77, respectively, and were significantly lower (P 0.05) than P0 or P1. It was concluded that semen extended using tris-egg yolk extender supplemented with vitamin A or C could still be used for Artificial Insemination until day 4 after extended and storage at 5°C. It is suggested that research needs to be done to produce a ready to use, simple preaparation, cheap and long lasting instant extender.

Intracervical Artificial Insemination using Frozen Semen of Boer buck on Pote Does at Different Times of Estrus

Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchronization with PGF2α of 200 µg intramuscularly, twice injections with 11 days intervals. The T1 groups of estrus the Pote does were inseminated at early estrus (0-12 hours). Meanwhile, T2 groups of estrus the Pote does were inseminated at mid-estrus (12-24 hours after signs of estrus appeared. The insemination conducted artificially using post-thawed Boer semen. The observed variables were non-return rate at day 21 post-insemination (NRR21), and pregnancy rate (PR) based on abdominal palpation diagnosis. The result showed that post-thawed Boer buck was qualified (sperm motility more than 40%) for artificial insemination. All of the Pote does were estrus after twice PGF2α intramuscular injection with 11 days intervals. The onset of estrus was 48-72 hours after the second injection PGF2α. The NRR21 and PR ranged from 66.66-100% and 37.50-66.66%, respectively. The NRR21 and PR of the T2 group were higher than those of the T1 group. It could be concluded that intracervical artificial insemination on Pote the does at mid-estrus were more effective, resulting in higher NRR21 and PR than those at early estrus.

Intracervical Artificial Insemination using Frozen Semen of Boer buck on Pote Does at Different Times of Estrus

Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchronization with PGF2α of 200 µg intramuscularly, twice injections with 11 days intervals. The T1 groups of estrus the Pote does were inseminated at early estrus (0-12 hours). Meanwhile, T2 groups of estrus the Pote does were inseminated at mid-estrus (12-24 hours after signs of estrus appeared. The insemination conducted artificially using post-thawed Boer semen. The observed variables were non-return rate at day 21 post-insemination (NRR21), and pregnancy rate (PR) based on abdominal palpation diagnosis. The result showed that post-thawed Boer buck was qualified (sperm motility more than 40%) for artificial insemination. All of the Pote does were estrus after twice PGF2α intramuscular injection with 11 days intervals. The onset of estrus was 48-72 hours after the second injection PGF2α. The NRR21 and PR ranged from 66.66-100% and 37.50-66.66%, respectively. The NRR21 and PR of the T2 group were higher than those of the T1 group. It could be concluded that intracervical artificial insemination on Pote the does at mid-estrus were more effective, resulting in higher NRR21 and PR than those at early estrus.

Correlation Beetwen Body Weight with Scrotal Circumference, Testis Weight and Sperm Production of Boer Buck Intensively Rearing

Body weight of one of the regulators that determine the scrotum circumference and weight (large) testicles. Next, the weight of the testes has a very close relationship with sperm production that will determine the reproductive ability of a male. The purpose of this study was to determine the relationship between body weight and scrotum circumference, testicular weight and boer goat sperm production that was maintained intensively. The study was conducted with a descriptive method with the material of 15 male goats owned by PT. Sadhana Arif Nusa. Data is processed by regression and correlation analysis using the SPSS program. The results showed that the body weight average was 52.58 ± 14.96 kg, correlated very significantly (p &lt;0.01) with scrotum circumference (25.68 ± 1.96 cm) and testicular weight (0.46 ± 0, 10 kg) with a correlation coefficient of 0.67 and 0.91. Body weight not correlated (p&gt; 0.05) with good sperm production volume (0.50 ± 0.19 ml), pH (6.43 ± 0.19), sperm consistency, progressive motility (73.33 ± 7, 24 %) and Spermatozoa concentration (2.18 ± 0.54 x 109/ml). The scrotum circumference is very significantly correlated (p &lt;0.01) with a testicular weight and a real correlation (p &lt;0.05) with sperm volume, motility and concentration of spermatozoa with a coefficient of 0.91 each; 0.58; 0.56 and 0.54. The testes weight is real correlating (p &lt;0.05) with sperm volume, progressive motility and spermatozoa concentration with a coefficient of 0.53 each; 0.56 and 0.54. CONCLUSION, body weight correlates piosif with scrotum circumference, the weight of the quality of the testes and sperm production in boer goats.

Reproductive performance comparison between natural and artificial service in Jawarandu goat

This study was aimed to compare reproductive performance of Jawarandu goat serviced naturally and artificially. In total 433 Jawarandu does were randomly allocated into two groups namely Natural Mating (NM; n=233) and Artificial Insemination (AI; n=200). In this study, reproductive performance was measured in percentage of pregnancy, litter size, and kid born single, twin and triplet. Between groups, data were compared using t-test at α = 5%. NM group was performed in mating pen, and per Boer buck was colonized with 20-25 does for 45 days, followed with pregnancy check using ultrasonography at day 45 post insemination. In AI group, oestrous signs were observed in morning and evening, while insemination was done maximum 12 hours after sign of oestrous. The percentage of pregnancy in NM was higher (p<0.05) compared to AI (73.39 vs 21.00%), litter size (1.37±0.49 vs 1.35±0.56; p>0.05). Similarly, in percentage of kid born single, twin and triplet, no differences were found in both groups (63.35 vs 64.86; 36.02 vs 35.14; and 0.62 vs 0%; respectively). All in all, natural service gives better reproductive performance compared to the artificial one. Its suggested due to factors related to the ability to determine the right time for service.

BoerPE Goat Blood Profile as Indicators of Crossbreeding Resistance

Cases of clinical reproduction in crossbred cattle have been reported with the incidence of decreased reproductive performance. Crossbreeding resistance is usually followed by low immunity. Blood profile is one of the parameters that illustrate the immunity of livestocks. Goat crossbreeding resistance has never been studied. The purpose of this study was to analyze the crossbreeding resistance of the F1 BoerPE goat and her mothers by analyzing their blood profile. F1 BoerPE goat is the offspring of crossbreeding between Boer buck with PE goat. The research method was case study. The primary data were obtained from examination of blood profile and Physiological Status of F1 BoerPE goat and her mothers. The secondary data obtained from recording age and body weight. The blood profile data were analyzed by t test. The observed blood profile indicators were neutrophils, monocytes and lymphocytes. The results showed that the physiological status of F1 BoerPE goat and her mothers was not significant. Nevertheless, the number of neutrophils, lymphocytes and monocytes was significantly different (p˂0.01). The average (%) number of neutrophils, lymphocytes and monocytes of PE goats was 37.92 ± 1.37; 51.85 ± 1.4; 3.87 ± 0.21. while the average blood profile of F1 BoerPE was 46.16 ± 2.1; 58.06 ± 3.4; 5.51 ± 0.18. The average number of neutrophils, lymphocytes and monocytes F1 BoerPE goat was higher compared to the parent. It is indicated that there were crossbreeding resistance of F1 BoerPE goat.

Kidding rate of artificial insemination with Boer goat liquid semen during chilled preservation using coconut water-based diluent

The quality of liquid semen with coconut water as diluent could be preserved up to 3 to 4 days during cold storage at 4 - 5oC. In this research, the successful insemination rate of coconut water-based diluent Boer goat liquid semen was observed to understand the application of coconut water as semen diluent. The materials used in this research were 5 to 7 months Viridis coconut water, liquid semen from 3 years old Boer buck, 30 does for acceptor, and Tris-aminomethane added with 10% egg yolk (EY) as a control diluent. The Boer semen was collected once a week by using artificial vagina, diluted and then stored before used for insemination. The insemination was done by using intra-cervical method at position 3, started with estrous synchronization by using 2 mL PGF2α. The research was conducted as experimental method under randomized group design with two treatments, that were Tris-aminomethane + 10% EY (P0) and coconut water + 10% EY (P1). All of the treatments were repeated for 15 times and the collected data were analyzed with Pearson’s chi square with Genstat 18 program. The observed variables include non-return rate and kidding rate. The results showed that the first non-return rate of P1 were 93.33% and in P0 were 73.33%, while the second non-return rate of P1 were 93.33% and in P0 were 80%. Furthermore, the kidding rate of P1 were 40% and P0 were 66.66%. The Pearson’s chi square analysis showed that there was no effect of coconut water as semen diluent to the kidding rate. The research concludes that the usage of coconut water as semen diluent did not affect the successful insemination rate of Boer goat.

Percentage Viability Spermatozoa in Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender

Percentage Viability Spermatozoa in Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender

Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen

This study aimed to determine the proper sperm dose per laparoscopic intrauterine insemination (LAI) in Zaraiby goats. Thirty-six Zaraiby goats, two Zaraiby and two Boer bucks were used in the study. Does were divided into six groups; the first three groups were inseminated laparoscopically with frozen-thawed Zaraiby semen either at doses of 10 x 106 (GP1), 20 x 106 (GP 2), or 40 x 106 (GP 3). The other three groups were inseminated with Boer semen's same doses (GP 4, 5 &6). Results showed that goats laparoscopically inseminated with 20 x 106 Zaraiby spermatozoa or 10 x 106 Boer sperm cells both had the highest (P ≤0.05) pregnancy rate (about 70% & 60%, respectively). The multiple birth rate in goats inseminated by Boer buck’s spermatozoa were generally higher than those inseminated with Zaraiby buck’s spermatozoa (66.67 to 100.00% vs. 25.00 to 40.00%, respectively). In conclusion, the LAI is an efficient means of achieving high fertilization rates in goats. The recommended minimum necessary dose for laparoscopic artificial insemination in Zaraiby is 10 ×106 and 20×106 motile Boer and Zaraiby buck’s spermatozoa, respectively.

Total Bacteria of Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender

Aims: To determine the effect of the addition of sweet orange essential oil to the extender (tris yolk extender without the addition of antibiotics/antibacterial) to total bacteria Boer Buck frozen semen.&#x0D; Study Design: Randomized Block Design.&#x0D; Place and Duration of Study: Sample: Laboratory Reproduction of Loka Penelitian Kambing Potong Sei Putih Indonesia, between January and March 2019.&#x0D; Methodology: The research procedure starts with the preparation of semen extender, collection of fresh semen, dilution of semen, equilibration, freezing of semen, and thawing. This research obtained conducted using a Randomized Block Design consisting of 5 treatment levels and five replications. Semen storage using 3 Boer buck, which done for three days. As a treatment is the addition of sweet orange essential oil as much as (P0) 0%, (P1) 0,25%, (P2) 0,5%, (P3) 0,75% and (P4) 1% on the tris yolk extender. The observed variables was total bacteria evaluated before freezing and after freezing (Post-Thawing).&#x0D; Results: The results showed that the addition of sweet orange essential oil had a very significant effect (P &lt;0.01). The results of adding sweet orange essential oil to the extender (Post-Thawing) were 78.6 x 102 CFU (P0), 76 x 102 CFU (P1), 73,2 x 102 CFU (P2), 71 x 102 CFU (P3) and 68.6 x 102 CFU (P4).&#x0D; Conclusion: The best values the total bacteria 68.6 x 102 CFU (P4). It can conclude that the best result of adding sweet orange essential oil to tris yolk extender is (P4) 1%.

Evaluation of Sperm Velocity Parameters with Glutathione and Honey in Skim Milk Based Extenders by CASA on Boer Buck

The study aimed to evaluate the Boer buck sperm velocity (μm/sec) parameters (VCL: Curvilinear Velocity, VAP: Average Path Velocity and VSL: Straight line Velocity) with 5 mM Glutathione (G) and 1% or 2% Honey (H) in Skim milk (SM) based extenders preserved at refrigeration temperature for 0, 24, 48 and 72 hrs. A total of 72 ejaculates were collected equally from 6 mature bucks at the weekly interval by using Artificial Vagina (AV) as per the standard procedure. All the ejaculates were diluted using six Skim milk-based extenders, viz. SME, SMGE, SMGH(1%)E, SMGH(2%)E, SMH(1%)E and SMH(2%)E. The sperm motility was evaluated by CASA (Computer Assisted Semen Analyzer). The data obtained was statistically analyzed. The results showed that sperm velocity parameters (VCL, VAP, and VSL) differed significantly (p less than 0.05) from the extender to extender at 24, 48, and 72 hours of refrigeration. Supplementation of optimum concentration of glutathione (5 mM) and honey (1%) maintained better sperm velocity parameters up to 72 hours of storage compared to other extenders and was successful in the preservation of buck spermatozoa at refrigeration temperature. Hence, It was concluded that Boer buck semen could be preserved effectively with SMGH(1%)E at refrigeration temperature for sperm velocity parameters up to 72 hours of storage.

Evaluation of sperm motility and velocity of different genetic groups of bucks using Computer-Assisted Sperm Analyzer

Semen quality assessment is a prime prerequisite for any breeding programme. Computer-Assisted Sperm Analyzer (CASA) can measure the semen quality very accurately and sharply. The research was conducted at American Dairy Limited (ADL) at Vangnahati, Sreepur, Gazipur. A total of 120 ejaculates were collected from three genetic groups of bucks namely Black Bengal (BB), Boer and Jamunapari (JP) with a view to characterize and compare the semen motility and velocity parameters using Computer- Assisted Sperm Analyser. Significantly (p&lt;0.05) higher ejaculate volume was found in Boer buck (1.73±0.16 ml) with the lower in BB buck (0.98±0.14), respectively. But significantly (p&lt;0.05) higher total, forward progressive and fast motility was found in BB (55.47±3.17, 47.23±2.74 and 37.62±2.40%) and lower in JP (40.67±3.37, 30.97±2.91 and 21.81±2.55%), respectively. Significantly (p&lt;0.01) fastest straight line and average path distance was observed in Boer bucks (12.29±1.63 and 15.89±1.95 μm) than Black Bengal and Jamunapari bucks (4.38±1.19 and 7.26±1.57; and 3.14±1.27 and 8.42±1.95 μm), respectively. The distance curved line (DCL) and distance straight line (DSL) was significantly (p&lt;0.05) higher in Boer (29.13±4.87, 12.29±1.63 and 15.89±1.95 _m/sec) and lower in BB (22.07±4.36 and 7.26±1.57 _m/sec), respectively. In case of curvilinear velocity, straight line velocity and average path velocity value was significantly (p&lt;0.01) higher in Boer (127.08±13.88, 50.01±7.68 and 68.13±7.25 _m/sec) with lower in JP (62.01±12.77, 15.75±7.35 and 22.39±9.42) where as linearity and straightness was higher in Boer and lower in BB (p&gt;0.05), respectively. The scrotal circumference had positive correlations with semen volume and curvilinear velocity for all the genotypes. It was concluded that semen of Boer bucks found consistently better than other two genetic groups of bucks. Further, competitive studies with large sample may be performed to evaluate the breed specific differences in available goat breeds. I think this study also disclosed the some rare parameters of semen quality by using Computer-Assisted Sperm Analyzer which was impossible in manually. These results of the present study could be an authentic guideline in future performance tests of different genetic groups of bucks.&#x0D; Bang. J. Anim. Sci. 2019. 48 (2): 67-74

Influence of butylated hydroxytoluene addition to cryodiluents on freezability and DNA integrity of Boer and Zaraibi buck spermatozoa

Objective: To evaluate the cryoprotective effect of butylated hydroxytoluene on buck frozen semen. Methods: Semen was collected from Boer (n=6) and Zaraibi (n=6) bucks by electroejaculator for 5 weeks. Semen aliquots were diluted at 38 °C in Tris-buffer with egg yolk 15.0% (vol/vol) (Tris- egg yolk extender) or soya lecithin 2.5% (weight/vol) (Tris-soya lecithin extender) supplemented with butylated hydroxytoluene at 0.0 (as the control), 0.5, 1.0, 2.0 and 4.0 mM. Post-thawing motility (at 400x magnification), plasma (hypo-osmotic swelling test), acrosome (Trypan blue/Giemsa dual staining) membranes, DNA (comet assay), and lipid peroxidation (by malondialdehyde concentration) were assessed. Results: Spermatozoa motility was enhanced by butylated hydroxytoluene in Tris-soya lecithin extender at 0.5 mM in the two breeds, and in Tris-egg yolk extender at 1.0 mM in Boer and at 2.0 mM in Zaraibi bucks for up to 3 h post-thawing. Plasma and acrosome membranes and DNA integrity of the two breeds were maximally high with butylated hydroxytoluene at 1.0-2.0 mM in Tris-egg yolk extender and at 0.5-1.0 mM in Tris-soya lecithin extender. Lipid peroxidation was minimal with butylated hydroxytoluene at 1.0-2.0 mM in Tris-egg yolk and soya lecithin extenders in the two breeds. Butylated hydroxytoluene at 4.0 mM deteriorated spermatozoa motility, and plasma and acrosome membranes. Conclusions: The consequence of butylated hydroxytoluene on buck frozen-thawed spermatozoa varies with the levels of supplementation, buck breed, and phospholipid source in the extender. Semen parameters of Boer buck are better in their response to butylated hydroxytoluene than Zaraibi buck. Butylated hydroxytoluene at 1.0 and 2.0 mM in Tris-egg yolk extender, and at 0.5 mM in Tris-soya lecithin extender represents the best concentrations and profitably improves the semen quality of buck semen.

Post-thawing sperm quality of Boer buck semen diluted in phosphate buffer saline supplemented with bovine serum albumin

This study aimed to determine post-thawing sperm quality of Boer buck which diluted in Phosphate Buffer Saline (PBS) medium supplemented with Bovine Serum Albumin (BSA) for optimizing its quality in addressing In Vitro Fertilization (IVF) purposes. In this research thawed semen was diluted in PBS with the addition of three different BSA concentration namely T0 (BSA 0%), T1 (BSA 0.1%), and T2 (BSA 1%). Semen and sperm quality such as motility, viability and abnormality (all in %) were observed at initial thawing and after diluted with PBS and BSA before and after incubation. At initial thawing, semen characteristic shows motility at 62.67±7.03%. Meanwhile, in all sperm quality after dilution at T0, T1 and T3 shows decreased after incubation time, although no differences found between BSA concentration (P>0.05). From this study, it is concluded that adding BSA as complementary nutrition in PBS medium could maintain sperm quality at affordable level for further IVF purpose.

Telaah potensi hybrid vigor sifat bobot badan pada silangan kambing Boer dan Jawarandu

Crossbreeding program was an option in order to improve Indonesian local goat productivity. In general, crossbreeding is aimed to utilize the benefit ofheterosis (hybrid vigor: HV); where better offspring performance compared to its parents phenotype is expected. Currently, crossbreeding between Boer bucks and Jawarandu does is widely practised; resulting in a crossbred called Boerja. However, information regarding the HV effect were less available. Thus, this study aims to calculate HV in Boer × Jawarandu in relative to the mid-parental average, and relative to the dam phenotype. Mature body weight (BW) of 504 head of goat derived from Boer buck (n=6), Jawarandu doe (n=15), Boerja F1 (n=298), Boerja F2 (n=167), and Boerja F3 (n=18) were analyzed. Backcross system was applied to produce Boerja F2 (Boer ♂ × Boerja F1 ♀) and Boerja F3 (Boer ♂ × Boerja F2 ♀). Result shows that BW in Boer, Jawarandu, Boerja F1, Boerja F2, and Boerja F3 were 79,00 ± 16,21; 29,07 ± 3,76; 41,84 ± 4,12; 41,69 ± 3,68 and 39,96 ± 4,97 kg respectively. The heterosis value of Boerja F1, Boerja F2, and Boerja F3 in relative to the parents average were -23, -31, and -34%, while in relative to the dam were 44, 0, and -4% respectively. According to HV value, it is concluded that the most optimum result of Boer × Jawarandu crossbreeding was found in Boerja F1. Higher Boer breed proportion in F2 and F3 were found to have lower BW.

Treatment of cerebrospinal nematodiasis in a Boer Buck

A nine-month-old Boer Buck presented for an acute onset of pelvic limb ataxia. Radiographs, complete blood count and serum biochemistry were within normal limits. A cerebrospinal fluid analysis yielded an...

Prospect Produksi Pra Sapih Anak Kambing Hasil Silang Kambing Pe Dengan Kambing Boer

The study aims to evaluate the pre-weaning production prospectus kids of PE crossed with Boer buck. Twenty PE does were in the average 39.35 kg body weight and 3 in body condition score. Does were offered single feed sesbandia glandiflora leaves twice a day. All does were synchronized by 2 ml Capriglandin, inseminate fix time at 48-52 hours following Capriglandin injection. If the does were return to estrus following AI, then they were allowed natural mating. Data collected included gestation period, birth weight and average daily gain (ADG), tabulated and calculated for Mean±SD, interpreted and explained descriptively. The number of kid were 34 consisting of 6 singles male (SM), 12 single female (SF), 8 male twin (MT), 2 female twins (FT) and 6 male-female twins (MFT). Gestation period (days) were 152±0.47 and 152±2.00 for SM and MT. Shorten gestation period were noted for SF, FT and MFT (149±3.82; 150±3.94 and 147±2.94, respectively). The highest birth weight (kg) was SM (4.27±0.29) followed by SF (3.52±0.27), MFT (2.97±0.44), FT (2.73±0.23) and MT (2.71±0.54). The highest ADG (gr/day) occurred at 0-30 days was in MT (201.33 g/d) and the lowest was in FT (137.33 g/d). In conclusion, crossbreeding kid of PE VS Boer goat has a high prospectus as dual purpose stockbreeder