Body Reserves Research Articles

Food availability has direct and delayed effects on structural growth and body reserves in garter snakes.

Phenotypic plasticity in body growth enables organisms to cope with unpredictable paucities in resource availability. Growth traits influence survival and reproductive success, and thereby, population persistence, and early-life resource availability may govern lifetime patterns in growth, reproductive success, and survival. The influence of early-life environment is decidedly consequential for indeterminately growing ectotherms, which rely on available resources and ambient temperatures to maximize fitness throughout life. Using 17 years of mark-recapture data, we evaluate the effects of resource availability on patterns in growth for populations of western terrestrial garter snakes (Thamnophis elegans), which differ along pace-of-life continuums into fast- and slow-living ecotypes. We use an adaptation of the von Bertalanffy estimator to fit structural growth models and linear predictors for body condition to analyze the consequences of annual and early-life prey availability. Snakes from resource-poor early-life environments are primed to exploit conditions in high-prey environments later in life. Slow pace-of-live animals exhibit a greater capacity for compensatory strategies in structural growth, while body condition was best explained by a complex interaction across males and non-gravid females between prey availability and ecotype. Our findings highlight the importance of accounting for context-dependent early-life environments as well as sex-specific reproductive demands when evaluating population traits.

Effects of including a palmitic acid-enriched supplement in low and high starch diets on milk production and energy partitioning of primiparous and multiparous dairy cows between mid and late-lactation.

The aim of our study was to assess the effects of low or high-starch diets with or without palmitic acid (C16:0) supplementation on the yield of milk, milk components, and energy partitioning of primiparous and multiparous dairy cows between mid and late-lactation. Thirty-two Holstein cows, 12 primiparous ([mean ± SD] 163 ± 33 d in milk) and 20 multiparous ([mean ± SD] 179 ± 37 d in milk), were used in a split-plot Latin square design. Parity was considered the main plot, and within each plot, treatments were then randomly assigned in a replicated 4 × 4 Latin square with 21 d periods and balanced for carryover effects. Treatments were in a 2 × 2 factorial arrangement of dietary starch level and fatty acid (FA) supplementation as main factors. The starch factor comprised either low (LS; 16% of diet dry matter [DM]) or high (HS; 32% of diet DM) dietary starch. Dietary starch was altered by varying the proportion of ground corn, whole cottonseed, and soyhulls, with LS and HS diets containing ∼15 and 18% forage neutral detergent fiber (fNDF) and ∼37 and 25% neutral detergent fiber (NDF), respectively. The FA supplementation factor consisted of either control with no FA supplementation (CON) or a C16:0-enriched supplement at 1.5% of diet DM replacing soyhulls (PALM). Compared with LS, HS increased plasma insulin (0.20 μg/L) and energy partitioning to body reserves (2.04 percentage-units), tended to increase body condition score (BCS) and body weight (BW) change, and decreased dry matter intake (DMI; 1.10 kg/d), milk fat yield (0.04 kg/d) and milk urea nitrogen (MUN; 2.10 mg/dL). Compared with CON, PALM increased energy output to milk (1.10 Mcal/d), the yields of milk fat (0.10 kg/d), 3.5% fat-corrected milk (FCM; 1.90 kg/d) and energy corrected milk (ECM; 1.60 kg/d), and had no effect on BW-related variables. We observed a 3-way interaction between parity, starch, and FA supplementation affecting multiparous but not primiparous cows, where PALM increased plasma insulin concentration (0.14 μg/L) in LS but had the opposite effect in HS by tending to decrease insulin. Our results demonstrate important interactions between parity, starch levels, and FA supplementation. Overall, feeding a low-starch diet to mid and late-lactation dairy cows helped prevent excessive body condition and weight gain while increasing milk fat yield compared with a high-starch diet. Multiparous cows may benefit the most from a low-starch diet as multiparous cows seemed more prone to accumulating body reserves. Feeding a C16:0-enriched supplement increased milk fat yield and ECM without increasing weight gain, regardless of starch level or parity.

AS CITOCINAS IL-1B E TNF-A E O PERFIL METABÓLICO EM VACAS COM HIPERCETONEMIA: UMA REVISÃO DE LITERATURA

Hyperketonemia is the most prevalent metabolic disease in the transition period of productive cows. It is characterized by the increase of ketone bodies in body tissues and fluids, in addition to changes in metabolic profiles and cytokine expression, which can result in significant economic losses and damage to animal health. Objective: To conduct a literature review addressing the main aspects related to hyperketonemia, with emphasis on changes in the metabolic profile and expression of the cytokines IL-1β and TNF-α during the transition period. Methodology: The review was based on articles found in the databases, published between 1972 and 2024, using specific terms such as "hyperketonemia", "transition period" and "metabolic profile". Literature review: Hyperketonemia develops in response to negative energy balance, when energy demand exceeds dietary intake, leading to mobilization of body reserves and increased non-esterified fatty acids (NEFAs) in the blood. These fatty acids are metabolized in the liver, favoring ketogenesis and resulting in metabolic disorders. The metabolic profile is an essential tool to assess the metabolic health of the herd, allowing the identification of changes in biochemical parameters, such as glucose, NEFAs, proteins and hormones. This monitoring reveals the relationship between metabolism and inflammatory responses, and is crucial for the early detection of disorders. The cytokines IL-1β and TNF-α, released during inflammatory processes, play an essential role in the transition period of dairy cows, contributing to the amplification of the inflammatory response associated with hyperketonemia. Conclusion: Early detection through biomarkers, such as β-hydroxybutyrate and NEFAs, is essential to prevent hyperketonemia. Inflammatory alterations, such as the elevation of IL-1β and TNF-α, reinforce the importance of integrated strategies for nutritional, genetic and immunological management, aiming to minimize damage to animal health and reduce economic losses.

Modeling feed efficiency over productive lifetime and integrating a submodel for body reserve management in Nordic dairy cattle.

Genetic enhancement of feed efficiency can improve the economic sustainability and environmental responsibility of dairy farming. Although genetic selection holds promise for improving feed efficiency across the lifespan of dairy cows, comprehensive data spanning whole lactations or even a productive lifetime are currently limited. To address this, we used production data and data from a camera-based feed intake and BW recording system, along with records of production, feed intake, and weight on Holstein cows from a research herd. We aimed to estimate variance components for a multivariate, multiparity model of production, feed intake, and BW data to calculate genetic residual feed intake (gRFI) for each of the Nordic breeds (Holstein, Jersey, and Red Dairy Cattle). Our approach included investigating a new definition of energy balance (EBbody) calculated from changes in body reserves, serving as an energy sink in gRFI. The data in our analysis consisted of 4,751 Holstein cows (7,851 lactations), 2,068 Jersey cows (3,486 lactations), and 3,235 Red Dairy Cattle cows (5,419 lactations). We used Gibbs sampling to estimate posterior means and SD for all model parameters. Our findings revealed moderate lactation-wise heritability of gRFI (0.15-0.38) across all breeds and parities. Moreover, gRFI genetic correlations varied (-0.2 to 0.4) between early- and mid- to late-lactation stages across all breeds, and for lactation-wise gRFI, there were moderately high genetic correlations (0.39-0.59) between primi- and multiparous lactations across the 3 breeds. Those results suggest the importance of recording phenotypes in most time periods within and across lactations. Our analysis indicated that improving gRFI with one genetic SD unit corresponded to a 2% to 3% gain in net return profit per cow-year, with no or minimal impact on production and body reserve management. We demonstrated the feasibility of incorporating EBbody into gRFI. Comparing gRFI calculated with EBbody or changes in BW as an energy sink trait for body reserve management were highly genetically correlated (>0.95). This result shows that the choice of the energy sink trait for body reserve management in gRFI will yield limited reranking among cows and sires when based on BW records only. However, EBbody offers an opportunity to incorporate BCS information without increasing the number of genetic parameters to be estimated, but it relies on parameters estimated in experimental settings. In conclusion, our study demonstrates the feasibility of developing a model for gRFI over most of the productive lifetime of dairy cattle, offering significant economic benefits without compromising productivity or body reserve management. Moving forward, comprehensive recording schemes covering whole lactations and productive lifetimes are advantageous for accurate selection indices of gRFI.

Feed Efficiency and Surplus Feed Intake among Norwegian Red Dairy Cows.

Feed in livestock production is a major cost driver and closely linked to GHG emissions from the sector. Hence, there is a lot of interest to improve feed efficiency of livestock and promote economic and environmental sustainability of livestock production. Residual feed intake (RFI) is one common indicator of feed efficiency of dairy cows. However, RFI has both conceptual (e.g., confounding the effects of measurement error and unobserved heterogeneity) and practical limitations (e.g., negative values and non-monotonicity with feed efficiency). Therefore, we estimated feed efficiency of 48 early- to mid-lactation Norwegian Red dairy cows based on intake and performance data collected for 54 d using a stochastic feed requirement frontier (SFRF). The SFRF predicted the minimum feed intake each cow required to support its production and maintenance needs, accounting for parity, lactation stage, body reserve mobilization, unobserved cow-specific heterogeneity, and statistical noise. Feed efficiency of each cow was then estimated in 2 ways: first by computing a feed efficiency score as a ratio of the prediction from the SFRF to the observed feed intake of each cow, and second by computing surplus feed intake (SFI) of each cow as a difference between observed and predicted feed intake. Furthermore, it was shown that feed efficiency can be decomposed into time-invariant and time-varying parts. Results showed that the average feed efficiency score was 89.7% and it was time-invariant for the period covered during the study. The score implies 10.3% of the observed feed intake was SFI. In absolute terms, the SFI amounted to 13.3 kg DM/wk on average. However, cows in the least feed efficient quartile had SFI of almost 5 times higher than cows in the most feed efficient quartile. Furthermore, cow-specific feed intake variation accounted for only 53.9% of the total feed intake variation. The remaining 46.1% of the feed intake variation was due to statistical noise. Out of the cow-specific feed intake variation, 82.1% was due to differences in feed efficiency while the remaining 17.9% was due to other sources of unobserved cow-specific heterogeneity. When implemented with lifetime data, the feed efficiency decomposition approach presented in this study enables understanding of the temporal trajectory of feed efficiency within and across lactations. Furthermore, it allows identification of feed efficiency that is repeatable over lifetime. facilitating the inclusion of feed efficiency in breeding programs.

Pregnancy affects maternal performance, feed intake, and digestion kinetics parameters in beef heifers.

This study aimed to quantify the effects of physiological status (PS) and potential interaction of this factor with days of pregnancy (DOP) on beef heifers' weight variation, intake, and digestion kinetics. Twelve rumen-cannulated zebu beef heifers (n = 7 pregnant; n = 5 non-pregnant) were used. Heifers were placed in individual pens and fed medium-quality corn silage plus a protein-based supplement. Heifers' body weight was assessed at the beginning and end of each collection period. The feed intake was measured daily. Target outcomes were evaluated at 107, 170, 208, 240, 267, and 286 days of pregnancy. The apparent total-tract digestibility was determined considering the fecal spot collection, during five days within each collection period. Omasal and ruminal digesta samples were collected at 107, 208, and 267 days of pregnancy for determination of partial digestibilities and diet component outflow. For these purposes, Co-EDTA and indigestible neutral detergent fiber (iNDF) were used as indicators. All data were analyzed using a mixed model framework, considering the PS and DOP as fixed effects and the animal as a random effect. Significant differences were declared when P ≤ 0.05. Pregnant heifers showed an increase in body reserves (+35kg) from 107 to 240 days of pregnancy, but experienced a decrease in shrunk body weight (-36kg) from 240 to 286 days of pregnancy. The intake of dry matter (DM), organic matter (OM), crude protein (CP), and ash- and protein-free neutral detergent fiber (apNDF) increased as pregnancy progressed (P < 0.04). The apparent total-tract digestibility of DM tended to be lower (P = 0.09), and the apparent total-tract digestibility of apNDF was reduced (P < 0.01) in pregnant heifers. The digestibility of CP was higher (P < 0.01) on days 267 and 286 in pregnant cows compared to non-pregnant heifers. The ruminal digestibility of OM tended to be lower (P = 0.09) in pregnant beef heifers, while the ruminal digestibility of apNDF was lower (P = 0.02) in pregnant heifers compared to non-pregnant ones. The ruminal pool of wet matter and DM was lower (P ≤ 0.01) in pregnant heifers at 267 days of pregnancy. In all periods, the outflow tended to be greater (P = 0.06) in pregnant heifers compared to non-pregnant heifers. Pregnant beef heifers exhibited a faster (P = 0.01) digesta passage rate during late gestation. In conclusion, late-gestating beef heifers are less efficient in extracting energy from feed compared to non-pregnant animals.

Nosema ceranae infection reduces the fat body lipid reserves in the honeybee Apis mellifera

Nosema ceranae is an intestinal parasite frequently found in Apis mellifera colonies. This parasite belongs to Microsporidia, a group of obligate intracellular parasites known to be strongly dependent on their host for energy and resources. Previous studies have shown that N. ceranae could alter several metabolic pathways, including those involved in the nutrient storage. To explore the impact of N. ceranae on the fat body reserves, newly emerged summer bees were experimentally infected, and we measured (1) the lipid percentage of the abdominal fat body at 2-, 7- and 14-days post-inoculation (p.i.) using diethyl ether lipid extraction, (2) the triglyceride and protein concentrations by spectrophotometric assay methods, and (3) the amount of intracellular lipid droplets in trophocytes at 14- and 21-days p.i. using Nile Red staining. Comparing the three methods used to evaluate lipid stores, our data revealed that Nile Red staining seemed to be the simplest, fastest and reliable method. Our results first revealed that the percentage of fat body lipids significantly decreased in infected bees at D14 p.i. The protein stores did not seem to be affected by the infection, while triglyceride concentration was reduced by 30% and lipid droplet amount by 50% at D14 p.i. Finally, a similar decrease in lipid droplet reserves in response to N. ceranae infection was observed in bees collected in fall.

Comparison of manual and automated body condition scoring of commercial dairy cattle.

Body condition scoring of dairy cows estimates their body reserves. Automation allows increased data availability and reduced labour costs. The aim of this study was to compare an automated (AUT) body condition score (BCS) system to manual observers on a single commercial dairy farm in south-west England. Three practising veterinary surgeons performed body condition scoring of 315 dairy cows using the agriculture and horticulture development board (AHDB) Body Condition Scorecard. AUT BCSs were obtained from two 3D cameras and compared to the BCSs recorded by the three operators. The AUT system only agreed with manual scorers at a BCS of 3. The system failed to detect cows classified as underconditioned (BCS ≤ 2.25) by any of the operators (sensitivity 0%). It also systematically underestimated the BCS of cows classified as overconditioned (BCS ≥ 3.5) by the operators. For overconditioned cows, the sensitivity of the AUT system ranged from 30.7% to 48.8% when compared with the manual operators. The AUT system also had weaker agreement with operators for Jersey cows, with Cohen's weighted kappa values of 0.28 for Jersey animals and 0.40 for Holsteins. This study used a convenience sample of animals on a single farmat a single time point, so the extent to which the findings can be more widely generalised is unclear. The AUT system failed to detect animals classified as underconditioned by the operators and underestimated the condition of cows classified as overconditioned by the operators. Currently, without improvements to the algorithm, the clinical usefulness of such an AUT system for body condition scoring is limited.

Assessment of skeletal muscle dynamics and milk production across a 300-day lactation in multiparous dairy cattle

The objective of this study was to evaluate changes in longissimus dorsi muscle depth (LDD) across lactation (0 to 300 DIM) and identify the impact of low versus high muscle reserves immediately after parturition on body weight and body reserve changes as well as production variables across a 300-d lactation. Forty multiparous cows were classified as high muscle (HM; LDD > 5.0 cm; n = 18) or low muscle (LM; LDD ≤ 5.0 cm; n = 22) based on LDD measurements collected within 24 h of parturition. Body weights (BW) and ultrasound scans to assess LDD and back fat depth (BFD) were collected monthly from parturition until 300 DIM. Ultrasound scans captured and measured using available software. Blood samples were taken at 7, 150 and 300 DIM, and plasma was analyzed for markers of metabolic status by measuring insulin, nonesterified fatty acids (NEFA), creatinine, and 3-methylhistidine (3-MH). Milk yield was recorded daily and milk components were analyzed monthly. Data analysis was performed and the statistical models included the fixed effect of muscle group, time, their interaction, and the random effect of cow nested within muscle group with repeated measures using a first-order autoregressive covariance structure. Muscle group was not related with BW or BFD for any of the time points measured. Cows lost BW from 0 to 60 DIM and gained weight from 60 to 300 DIM. Similarly, BFD decreased between 0 to 90 DIM and increased BFD after 90 DIM until 300 DIM. A muscle group by time interaction was observed for LDD. The HM cows had more muscle at 0 DIM, indicative of treatment assignment (1.34 cm more), and 300 DIM (0.78 cm more) and tended to have more muscle at 60 DIM (0.66 cm more) compared with LM. After 240 DIM, both muscle groups began net accretion of muscle reserves until 300 DIM. No differences were observed for blood metabolites measured based on muscle group. However, there were significant time effects for creatinine, 3-MH, and NEFA concentrations, which reflected the observed changes in BFD and LDD measured in ultrasound scans. For statistical analysis of daily milk production, observations were grouped into 3 stages of lactation, early (0-60 DIM), mid (60-240 DIM), and late lactation (240-300 DIM). There was a muscle group by stage of lactation interaction, where in early and mid-lactation, HM cows produced, on average, 1.9 kg more milk/d; however, in late lactation, LM cows produced 1.8 kg more milk/d. Our results indicate that muscle reserves are depleted in early lactation, and accreted in late lactation, whereas BW and BFD started to increase by 90 DIM. Data also supports that cows with more extensive muscle depletion in early lactation had greater milk production, however, substantial muscle accretion in late lactation may result in reduced milk production.

The Establishment of Geriatric Multidisciplinary Service (GEMS) for the Identification and Management of Frailty and Delirium in Emergency Care

Abstract Background In Ireland the number of people aged 75 and over has grown by greater than 20% in recent years, as a consequence increasing numbers of older adults are presenting to emergency departments. Frailty is a multi-factorial syndrome that diminishes body reserves and impacts on the person's ability to respond to stressors, such as illness or trauma. In October 2023, a Consultant Geriatrician lead GEMS was launched to identify, assess and manage frailty and associated syndromes, this study concentrated on delirium. Methods All patients 75 and older are triaged by GEMS using an electronic IPM System against prioritisation inclusion and exclusion criteria, utilising a case management approach and Rockwood Clinical Frailty Scale to screen for frailty. CFS score 1 to 3 is fit and managing well, alternatively a CFS of 4 or greater initiates a Comprehensive Geriatric Assessment. A 4AT assessment identifies patients at risk and screening for delirium. Results The results demonstrate a total of 744 patients were reviewed by GEMS from Jan - April 2024, 67% (n=503) had a CGA completed and 72% (n=537) of the population sample are living with frailty. A CFS7 (n=121), CFS8 (n=15) and CFS9 (n=3) are severely frail 26% and moderate frailty CFS6 (n=149) 28%. The 4AT results identified 10% of patients had a positive delirium score (n=60). In addition, a total of (n=61) patients had a formal diagnosis of dementia of which (n=12) patients had a positive 4AT. Conclusion The data confirms the prevalence of frailty in emergency care is approximately 70% and the incidence of acute delirium is approximately 10%. These indicators significantly impact on outcomes of care and treatment plans. Therefore, substantiates the need for early identification of frailty and delirium and the requirement for a highly specialised Consultant Geriatrician Lead (GEM) Service to ensure patients are on the right journey.

The prenatal assimilation of minerals and metals in nestlings of a small passerine bird

Although certain metals can reach their highest concentrations in tissues of newly hatched nestlings, their sources have yet to be fully elucidated. Evidence for the differentiation of body reserves, including some chemical elements, during avian embryonic life and early post-hatching period is limited mostly to studies on poultry. Here, we present novel findings on the bioaccumulation and egg-to-nestling transfer of 10 minerals and metals, including non-essential potentially toxic chemical elements (Cu, Ni Cd, Pb, Zn, Ag, Mg, Fe, Co and Ca) in the tissues of the gastrointestinal tract (GT), whole body (WB) and carcass (CA) of nestlings of a small passerine bird, the Eurasian Reed Warbler Acrocephalus scirpaceus, breeding in an intensively fertilized fishpond habitat. Our findings show that concentrations of metals that are relevant to contaminated systems (Cu, Ni, Cd, Pb, Zn, Fe and Co) are the highest in GT tissues across all the nestling age classes examined (from hatching to day 9 of life). The important question emerged from our findings: why the hatchlings were characterized by higher metal concentrations in GT, WB and CA compared to older nestlings. We suggest that the prenatal accumulation of chemical elements assimilated from egg contents, and their subsequent utilization for building various tissues/internal organs by the growing embryo, is a reasonable explanation for this finding. More specifically, the potential functional mechanism explaining the highest concentrations of some trace metals in hatchlings seems to be associated with an extremely high rate of utilization of residual yolk metal resources that survived from the embryonic until the post-natal period. On the basis of our own work and literature data, we identified a presumable significant discrepancy in the actual rate of egg-to-nestling and egg-to-embryo metal transfer due to the eggshell being ignored (a commonly occurrence), and only the egg contents being treated as the basic repository of chemical elements for developing embryos. Much more research is needed on the prenatal assimilation and subsequent accumulation of chemical elements in a developing avian embryo, representing both the transition from near-to-hatching embryos to hatchlings, and then to older nestlings.

Behavioral adaptation to seasonal resource scarcity by Caribou (Rangifer tarandus) and its role in partial migration

Abstract Animals living in seasonal environments have adopted a wide array of tactics used to deal with seasonal resource scarcity. Many species migrate between habitats to reach areas where food resources are more plentiful as an attempt to address energetic demands through foraging. We assessed the winter behavioral adaptations of Caribou (Rangifer tarandus), a large ungulate inhabiting Arctic and sub-Arctic regions known for seasonal resource scarcity. Movement rates of Caribou are the lowest of the year during winter, revealing 1 mechanism individuals use to reduce energy expenditures. However, migratory individuals moved nearly twice as much as nonmigratory individuals during winter, suggesting that migratory individuals rely more upon income (forage), whereas nonmigratory individuals rely more upon capital (bodily reserves). Lichens are the primary winter forage for large, migratory herds of Caribou, and migratory individuals experienced more than 2.5 times greater lichen cover than nonmigratory individuals. We documented that both groups slowed their movement in areas of greater lichen cover, suggesting increased foraging time in these areas. Movement rates were greater near villages, which may be suggestive of disturbance, but the effect was weak. Overall energy saved by reduced movement rates was modest. However, energy savings were 11% of daily body energy lost or 47% of the demands of early pregnancy, which potentially could affect individual condition and/or fetal growth if not offset by increases in forage intake.

Impact of feed efficiency on young beef animals on adult resilience: From field challenge experiments to modelling approaches

The relationship between feed efficiency at young ages and resilience as cows has been examined using an experiment that combines residual feed intake (RFI) evaluation of beef heifers and subsequent dietary challenge as cows. Feed efficiency of 569 heifers of 22 months of age was estimated during a 70-day period. Then a dietary challenge was applied on 303 of these animals after their first calving, half of them being fed 30 % below their expected needs from 10 days after calving up to the start of the grazing period (around mid-April), after which all the animals received the same management. When possible, the animals were kept until third lactation, giving a total of 514 lactations. Dam weight, dam BCS and calf weight were measured regularly, dam milk production was estimated three times during the lactation and resumption of cyclicity was recorded as well. The experimental data were analyzed using linear models including the effects of heifer efficiency (RFI <-0.4 = efficient; RFI >0.4 = inefficient) and interaction of heifer efficiency and diet group. The data were also used to calibrate a herd dynamics model representing management-nutrition and reproduction interactions at animal level. The experiment data showed that the efficient heifers were 18 kg heavier as cows when fed ad libitum (P = 0.04) but no difference was observed when restricted. Within both diets, heifers classified as efficient produced between 6 and 12 % less milk as cows than inefficient heifers depending on the lactation stage (P < 0.01), with a subsequent effect on calf weight. Finally, the resumption of cyclicity was one week shorter for the efficient animals when fed ad libitum but the opposite was observed within the restricted group, representing a complete ovarian cycle difference between diet groups within the efficient class (P = 0.001). In the lifetime modelling approach, two clusters of animals with different characteristics, including RFI, were simulated under both a non-restricted and restricted feeding environment. In this simulation, efficient heifers produced lighter cows with lower intake and higher body reserves from second calving until culling. Non-efficient heifers had better productive (kg of weaned calf) performance as cows independently of environment. The simulated reproductive performance of cows was equal for animals coming from efficient and non-efficient heifers under the non-restricted environment whereas efficient heifers perform better in the restricted environment, especially when parity increases. Both approaches showed that the interplay between efficiency at young ages and short- and long-term resilience depends on the environment. It can be concluded that the selection for efficiency at young ages has a negative impact on lactational performance as cows but not necessarily on reproductive resilience.

Effect of dietary protein for gestating sows on re-establishment of body reserves and impact on reproductive performance

This study aimed to investigate the effect of increasing dietary protein during gestation on sows’ ability to re-establish body reserves and evaluate the impact on reproductive performance of multiparous sows. The experiment was carried out in a commercial Danish herd and 431 sows were included and allotted to one of five dietary treatments. The treatment period extended from the time of service until the sows were moved to the farrowing section, approximately 5 d before expected farrowing. Experimental diets were isoenergetic and contained increasing concentrations of dietary protein (standardized ileal digestible [SID] lysine [Lys]; 3.37 to 6.39 g/kg). Sow body weight (BW) and backfat (BF) were recorded at service, on d 28 and 84 of gestation, and one to three days post-farrowing. Live and stillborn piglets and piglet birth weight were recorded. Sow body protein and fat were estimated; however, body fat estimations were considered inaccurate. Moreover, the feed and Lys to BW gain ratios were calculated. In early gestation, the BW and body protein gain of parity 2 to 3 sows increased linearly with increasing SID Lys (P < 0.001), while in mid-gestation BW gain had a polynomic response to SID Lys (P < 0.001), showing that BW gain was maximized at 5.0 g SID Lys/kg diet. Above the maximal BW gain sows’ body protein gain reached a plateau (P < 0.001) and Lys to BW gain ratio increased (P < 0.001), indicating oxidation of excess protein. In contrast, sow BW and body protein gain of parity 4 to 5 did not change with increasing SID Lys in most gestation stages, indicating that maternal growth was limited by energy intake. Sow BF gain did not change with increasing SID Lys, even though increasing SID Lys presumably increased the energy costs for protein retention or oxidation. Sow reproductive performances were not affected by dietary treatment, showing that fetuses are highly prioritized. In conclusion, sows of parity 2 to 3 re-established more BW and body protein than parity 4 to 5, when fed increasing dietary protein (SID Lys) during gestation. The dietary concentration of SID Lys did not affect sow reproduction in terms of litter size and average piglet birth weight; thus, SID Lys should be kept low during gestation until approximately one week prior to farrowing, and yet allow sows to re-establish sufficient body reserves for mobilization in the following lactation.

Scars from human-tree interactions: pollarding, pathogens, body reserves, and long-term response by Salix fragilis L. (Salicaceae) in Scodovacca, Italy

ABSTRACT Arboriculture provides opportunities for assessing long-term tree-environment interactions. We assessed the surrounding context, scars, re-sprouts, and absolute width-growth of twenty-four Salix fragilis trees pollarded by the grandfather, father, and son of one family covering 120 years, quantifying the effects of cut, microorganism-infection, and body-accumulated photosynthate on the pollards’ resprout-and-growth. We determined pollard diameter, age, scars-number (C) and basal area (CAB), median times pruned (Cmn), putrid holes number (H), cumulative length (HL), and length-average (HAV), and two indicators of remaining photosynthate (Q, and QAV). Response variables were number of re-sprouts (RN), re-sprout-basal area (RAB), and trunk’s Absolute Growth (AG; Classic (MLR), and Bayesian Multiple Linear Regressions (BMLR)). The pollards experienced similar incoming light, water, CO2, nutrients, and temperatures. Higher C-values for 40-year-old pollards produced higher H-values (Linear Regression, R2 = 0.54, p = 0.02; Bayesian Linear Regression: BF10 = 3.79, n = 8) suggesting more infection when cutting. RN slightly changed in response to life-long accumulated CAB, Cmn, H, and HAV (BMLR, BF10 from 1.10 to 2.82, n = 24). RAB slightly changed responding to C, H, HAV, Q, and QAV (BMLR, BF10 = 1.11; MLR: p < 0.05, n = 24). AG declined in response to Cmn (MLR, R2 = 0.38, p < 0.001, n = 24). Thus, under favourable conditions severely wounded-and-infected trees keep re-sprouting and growing, even if growth is reduced.

Annual Variation in Proximate Chemical Composition of Fish Sold in Ludhiana Market: Punjab, India

A study was carried out to investigate the nutritional composition of four different fish species (Wallago attu, Labeo rohita, Pangasianodon hypophthalmus, and Rastrelliger kanagurta) sourced from the inland and marine sector comprising both culture and capture fisheries marketed in Ludhiana fish market from September 2021 to August 2022, encompassing four distinct seasons: post-monsoon, winter, pre-monsoon, and monsoon. The proximate parameters including protein, lipid, carbohydrate, ash and moisture content were analysed following the standard methods. The moisture content across the four species ranged from 72.48% to 81.08% in selected fish. Lowest moisture content was recorded during the pre-monsoon season, contrasting with their highest levels during the monsoon season. Protein levels ranged from 9.72% to 18.36% across different seasons. Specifically, P. hypophthalmus displayed the lowest protein content (9.72±0.18%), while R. kanagurta exhibited the highest (18.36±0.18). W. attu, L. rohita, P. hypophthalmus, and R. kanagurta exhibited their lowest lipid content in flesh during the monsoon season, measuring at 0.69%, 1.59%, 9.33%, and 2.47%, respectively, contrasting with their highest levels during the winter season at 1.07%, 2.05%, 11.04%, and 4.05%, respectively. Across different seasons, carbohydrate content varied from 0.23% to 2.04%. R. kanagurta exhibited the lowest carbohydrate values; while L. rohita displayed the highest. Across different species, ash content varied from 1.62% to 4.34%, with the lowest observed in P. hypophthalmus and the highest in W. attu. Overall in fish muscle, water content reached its peak levels while muscle protein content decreased during the monsoon. The decline in protein and the rise in water content of the muscle were attributed to gonadal development and maturation, which depleted muscle protein reserves during the monsoon season. Inverse relationship between moisture and fat content in fish were observed, suggesting that fatty fish typically exhibit relatively lower moisture content. Lower levels of carbohydrate content are found in the total proximate composition of fish muscle, indicating that most of the glycogen in freshwater fish does not significantly contribute to body reserves.

Trait‐specific sensitive developmental windows: Wing growth best integrates weather conditions encountered throughout the development of nestling Alpine swifts

AbstractThe size and growth patterns of nestling birds are key determinants of their survival up to fledging and long‐term fitness. However, because traits such as feathers, skeleton and body mass can follow different developmental trajectories, our understanding of the impact of adverse weather on development requires insights into trait‐specific sensitive developmental windows. We analysed data from nestling Alpine swifts in Switzerland measured throughout growth up to the age of 50 days (i.e. fledging between 50 and 70 days), for wing length and body mass (2693 nestlings in 25 years) and sternum length (2447 nestlings in 22 years). We show that the sensitive developmental windows for wing and sternum length corresponded to the periods of trait‐specific peak growth, which span almost the whole developmental period for wings and the first half for the sternum. Adverse weather conditions during these periods slowed down growth and reduced size. Although nestling body mass at 50 days showed the greatest inter‐individual variation, this was explained by weather in the two days before measurement rather than during peak growth. Interestingly, the relationship between temperature and body mass was not linear, and the initial sharp increase in body mass associated with the increase in temperature was followed by a moderate drop on hot days, likely linked to heat stress. Nestlings experiencing adverse weather conditions during wing growth had lower survival rates up to fledging and fledged at later ages, presumably to compensate for slower wing growth. Overall, our results suggest that measures of feather growth and, to some extent, skeletal growth best capture the consequences of adverse weather conditions throughout the whole development of offspring, while body mass better reflects the short, instantaneous effects of weather conditions on their body reserves (i.e. energy depletion vs. storage in unfavourable vs. favourable conditions).

Effects of increasing dietary inclusion of high oleic acid soybeans on milk production of high-producing dairy cows

Recent research has highlighted the importance of dietary fatty acid profile of fatty acid supplements on production responses of high-producing dairy cows. Conventional soybeans contain ∼15% oleic acid and ∼50% linoleic acid whereas high oleic acid soybeans (HOSB) contain ∼70% oleic acid and ∼7% linoleic acid. We determined the effect of increasing dietary inclusion of roasted and ground HOSB on production responses of high-producing dairy cows. Twenty-four multiparous Holstein cows (50.7 ± 4.45 kg/d of milk; 122 ± 57 DIM) were randomly assigned to treatment sequences in a replicated 4 × 4 Latin square design with 21-d periods. Treatments were increasing doses of HOSB at 0, 8, 16, and 24% DM. The HOSB replaced conventional soybean meal and hulls to maintain similar diet nutrient composition (% DM) of 27.4 - 29.4% (NDF), 20.6% forage NDF, 27.5% starch, and 15.9 - 16.5% CP. Total fatty acid content of treatments was 1.65, 3.11, 4.52, and 5.97% DM, respectively. Pre-planned polynomial orthogonal contrasts included the linear, quadratic, and cubic effects of increasing HOSB. Increasing dietary inclusion of HOSB linearly decreased DMI and milk urea nitrogen and increased yields of milk, 3.5% fat corrected milk, energy corrected milk, and milk fat, and quadratically increased milk protein. The increased response to milk fat was due to an increase in preformed milk fatty acids. Due to the increase in milk component yields and decrease in DMI, there was an increase in feed efficiency. Increasing HOSB inclusion linearly decreased plasma BUN concentration and tended to decrease plasma insulin. Increasing HOSB had no effect on BW change or BCS change. In summary, increasing dietary inclusion of HOSB up to 24% DM increased production responses of high-producing dairy cows and did not affect body reserves.

Sediment dynamics and geomorphology in coastal areas affect long-term abundance of waders

Millions of migrating and wintering waders depend on coastal areas and estuaries for feeding and to replenish body reserves. These habitats are dynamic, with changing intertidal flats and sediments due to natural and climate-driven processes. The aims of this study were to examine whether physical conditions of intertidal flats such as geomorphology and sediment compositions could explain abundance of wader species, and furthermore whether changes in these conditions over time influenced changes in abundance of waders. In addition to physical structures such as datum (level), slope and sediment composition of intertidal flats, dynamic variables connected to tidal cycles such as distance of the waterline from the coast and duration of water cover were included. Penetration of the sea floor was measured as a proxy for sediment composition. The study was conducted in the Danish section of the Wadden Sea during 1981–1986 and 2008–2009 with additional data from 2020. The focus was on 12 widespread wader species. PCA analysis revealed that wader species were distributed in relation to tidal cycles such as duration of water cover and the position of waterline and to the physical structures, datum, slope and penetration, in decreasing order. With regard to the physical structures, pairwise comparisons of distribution of waders species showed that mean datum (level) was significantly different for 51 pairs of species out of 66 comparisons, mean penetration values differed for 34 pairs, and mean slope differed for 26 pairs of wader species. Long-term changes of physical structures of transects revealed that changes of densities of 10 wader species were significantly correlated with changes per year in one or more of the physical structures: seven wader species were significantly correlated with changes per year of mean datum (level) of transects (Lapwing was positively correlated while Avocet, Golden Plover, Grey Plover, Dunlin, Bar-tailed Godwit and Redshank were negatively correlated), five species with mean slope (Oystercatcher, Ringed Plover and Knut were positively correlated and Grey Plover and Redshank were negatively correlated), and three species with mean penetration values (sediment composition) of transects (Avocet and Bar-tailed Godwit were positively correlated and Lapwing was negatively correlated). The study shows that long-term changes of physical structures of intertidal flats affected abundance of wader species. The results are important for interpretation effects of erosion and accretion of coastal areas as consequences of natural and climate driven processes such as sea level rise and coastal squeeze.

Body Condition in Small Ruminants—Effects of Nutrition on the Hypothalamic–Pituitary–Gonad Axis and Ovarian Activity That Controls Reproduction

Nutritional status plays a vital role in regulating ovary activity. This regulation is mediated by the hypothalamus–pituitary–gonad axis and by effects exerted directly on the ovary. Therefore, to achieve the best reproductive performance, it is essential to know how the nutritional status affects the secretion of GnRH, gonadotrophins, and sex steroid hormones. Adequate body reserves and energy balance are critical for optimal reproductive performance in sheep and goats. However, over- or under-conditioned animals experience issues like extended anestrus, irregular ovarian cycles, and reduced conception. Body condition scoring allows for the evaluation of the relationships between adiposity, nutritional status, and fertility. Acute feed deficits briefly stimulate processes, but chronic restrictions suppress pulsatile LH release, disrupting ovarian function. The process of follicle development is a very complex one which involves intricate interactions between the pituitary gonadotrophins and metabolic hormones as well as between the locally produced factors by the ovarian somatic and germ cells including the IGF system and the TGF-β superfamily members. Genotype and nutrition are factors that have an impact on follicular development, and seasonal factors are also involved. This review will give a brief overview on how the body condition can be evaluated and the effects of nutrition on the hypothalamus–pituitary–gonad axis and ovarian activity, which are responsible for reproductive regulation. This paper presents a clear and reasonable summary of the pathway that runs from the nutritional status of small ruminants to ovarian activity through the hypothalamic–pituitary–gonadal axis. This review summarizes methods for body condition evaluation in small ruminants and evidence regarding acute versus prolonged nutritional impacts on the hypothalamic–pituitary–gonadal axis and ovarian activity controlling reproduction.