Body Odor Research Articles

From Beauty to Identity: Cultural Value Betangas Tradition in Sambas Malay Society

The purpose this research is to determine the significance of the Betangas tradition's values, meanings, and processions among the Sambas Malay population in West Kalimantan province. The examine method used is a qualitative research method with a descriptive research type. The examine approach employed is qualitative using a descriptive study design. The Betangas tradition is generally carried out when there is a wedding celebration, especially in the Sambas area. The purpose of the Betangas tradition is being replaced by grooming equipment for the bride and groom when approaching the wedding. This is because Betangas have the goal of reducing excessive body odor and cleansing the body of both the bride and groom who will carry out the wedding and the special assumptions of the Sambas people. This study obtained results in the meaning of Betangas as a form of gratitude to Allah Subhanahu Wa Ta'alla for the Sambas Malay community, who are mostly Muslim and as a request for the two bride and groom to live a household life. While the function of the Betangas procession is to reduce sweating and cleanse the body's unpleasant odor caused by human sweat. Generally, the Betangas tradition is carried out for the bride and groom, but it is possible that anyone can carry out this tradition. The Betangas tradition is carried out from generation to generation and is still strong in the Sambas region in the Sambas Malay community as local wisdom that is still alive amidst the currents of globalization.

ANTIBACTERIAL ACTIVITY OF BASIL LEAVES EXTRACT TOWARDS BACTERIA STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCUS EPIDERMIDIS IN DEODORANT SPRAY

Body odor is a significant problem and is often encountered in some individuals characterized by excessive odor. A deodorant spray made from basil leaf (Ocimum Basilicum L.) extract is a product used to treat body odor caused by Staphylococcus Aureus and Staphylococcus epidermidis bacteria. The purpose of this study is to assess the efficacy of deodorant spray and basil leaf extract on bacterial activity, as well as the optimal dose of deodorant spray for lowering the activity of these bacteria. Method the research was conducted experimentally, testing antibacterial activity using agar diffusion. The results of the deodorant spray preparation test showed that the preparation was homogeneous, organoleptically brown in color, had a distinctive smell, and had a liquid texture; the pH test results met the requirements for deodorant spray 4.5-6.5. The average diameter of the inhibition zones produced from each preparation were Staphylococcus epidermidis bacteria, positive control 7 mm, negative control 10.1 mm; formula 5% 12.3 mm; formula 10% 13.3 mm; formula 15% 12.7 mm maximum concentration in inhibiting bacteria is formula 10% by 13.3 mm, while the bacteria Staphylococcus Aureus positive control 13 mm, negative control 11.2 mm, 5% formula 13.1 mm; formula 10% 13.4 mm; 15% formula 13.9 mm maximum concentration in inhibiting bacteria is 15% formula 13.9 mm. Based on the inhibitory zone created by 10% basil leaf ethanol extract, Staphylococcus epidermidis bacteria had a 13.3 mm clean zone and Staphylococcus aureus bacteria had a 26.5 mm clean zone.

Development of Ethnobotany Materials to Support Basic Concepts of Science Learning in Higher Education

The local knowledge of the Doridungga Donggo community in searching for medicinal and food ingredients is a form of wisdom because its use follows ecological rules. The use of these plants can be in the form of plants with medicinal properties that are used for treatment by communities around forest areas as traditional medicine. The research aims to investigate medicinal ingredients from conventional plants of the Doridungga Donggo community, which can be used to strengthen science learning concepts. Data sources and information on research data sources were determined using purposive sampling techniques. Data sources were selected based on primary data and secondary data. Preliminary data was obtained from the results of site surveys and supported by secondary data from various literature on ethnobotany. The research was conducted in Doridungga Village, Donggo District, Bima Regency, NTB. Data was analyzed based on characteristic values, theoretical plant benefits, and plant benefits based on community perception. The research results include Bidara, Guava, Golkar, Turmeric, Betel, Pule, Coriander, Kicibling, and Rida Fada. These plants are used as medicine for fever, stomach ache, diabetes, stomach ulcers, to remove body odor and bad breath, tuberculosis, wounds, tonsils, urinary syphilis, headaches, and lumbago. The research results can be input for university science learning curricula based on local wisdom

Olfactory self-inspection: Own body odour provides cues to one's health and hygiene status

Olfactory self-inspection has been observed in various mammals, including humans. This behaviour can help individuals to monitor own state, including health and hygiene. This study's aim was to explore the frequency of sniffing particular body parts and investigate possible gender differences. Further, we tested a possible function of this behaviour, namely monitoring health and hygiene, by investigating associations between the frequency of self-sniffing and health, hygiene, and disgust sensitivity. Respondents completed an online survey on self-sniffing behaviour, health status, hygiene habits, and disgust sensitivity. Self-sniffing behaviour was investigated using a purpose-built inventory which explored the incidence and frequency of sniffing different parts of own body. Principal Component Analysis identified three main axes of self-sniffing behaviour: Social acceptability self-inspection, Intimate self-inspection, and Cosmetic self-inspection. Our results further show that respondents with lower standards of hygienic habits engage significantly more in intimate self-inspection (sniffing body parts such as genitals, anus, or navel). Interestingly, individuals who reported more frequent health issues sniff more frequently areas such as the armpits, feet, or own breath (Social acceptability self-inspection), probably to check for possible changes in smell due to illness. Our results indicate that olfactory self-inspection probably has several functions depending on the location from which the smell originates.

Review Artikel : Analisis Dan Formulasi Deodoran Berbahan Dasar Herbal Terhadap Kenyamanan Kulit

Body odour is a problem that interferes with daily life. The secretion of sweat glands and bacterial activity cause body odour. There are many ways to deal with the problem of body odour, including maintaining body hygiene and using skin care products such as deodorant and antiperspirant. The purpose of this review article is to determine the concentration levels of herbal-based active substances that are suitable for deodorant preparations and to determine their safety and comfort for the skin. This literature review article takes 4 main articles with 10 additional articles, spanning the last 10 years from 2013-2023. The results obtained from this review article show that all deodorant preparations have good formulation content. In spray-type deodorant preparations, the best formulation is deodorant with the active ingredient "Beluntas" leaf extract. In the roll-no type deodorant preparation, the best formulation obtained was with ethanol extract of “Mahkota dewa” leaves, carbopol base and triethanolamine. Both types of deodorant are safe and comfortable to use because they do not irritate the skin surface.

Examining differences in menstrual and intimate care product use by race/ethnicity and education among menstruating individuals.

United States consumers spend over two billion dollars a year on intimate care products. These products, along with scented menstrual products, are marketed for odor control, perceived "freshness," and vaginal/vulvar cleanliness. However, these scent-altering products may increase exposure to carcinogenic and endocrine-disrupting chemicals. Prior research has not adequately characterized demographic differences in product use. The objective of our study is to examine racial/ethnic and educational differences in menstrual and intimate care product use among people who menstruate. We pooled data from two US-based cross sectional studies to examine demographic characteristics and product use in 661 participants aged 18-54 years. Participants reported use of scented and unscented menstrual products (tampons, sanitary pads, and menstrual cups) and intimate care products (vaginal douches, sprays, wipes, and powders). We examined differences by race/ethnicity and education using log-binomial regression and latent class analysis (LCA), which can identify groups based on product use patterns. Our sample was 33.4% Black, 30.9% Latina, 18.2% White, and 16.2% another identity. Approximately half the population had a bachelor's degree or more; 1.4% identified as transgender and 1.8% as non-binary. In adjusted models, scent-altering products (i.e., scented menstrual and intimate care products) were more likely to be used by those with less formal education (p < 0.05). Unscented menstrual products were more likely to be used by those with more formal education. Compared to Black participants, White participants were more likely to use unscented tampons and menstrual cups and less likely to use douches and wipes (p < 0.05). Using LCA we identified two groups: one more likely to use scent-altering products, and a second more likely to use unscented menstrual products. Less education and older age, but not race/ethnicity, was significantly associated with membership in the group more likely to use scent-altering products. While sex/gender composition did not statistically vary across groups, all non-binary participants fell in the unscented menstrual product group. Lower educational attainment was consistently associated with greater use of scent-altering menstrual and intimate care products. Future research should examine associations between body odor stigma, product use, and health risks at intersections of race, class, and gender.

Theoretical Evaluation of Michelia Species’ Bioactive Compounds and Theraupetic Potential: A Literature Review

Michelia alba is a multifunctional plant found in Indonesia and is also called magnolia. Michelia alba is a tree known for its essential oil, which has long been used as a fragrance ingredient in perfumes and cosmetics. The potential of M. alba is immense for use in the prevention and treatment of disease as well as the management of human health and wellness. To date, there have been few publications regarding the biological activities of M. alba, focusing on tyrosinase inhibitory, antibacterial, antidiabetic, anti-inflammatory and antioxidant activities. However, M. alba may have additional undiscovered biological activities associated with its bioactive compounds. The purpose of this literature review research is to find out the bioactive compounds and therapeutic potential of the M. alba plant through literature searches that can be a reference for further research. The literature review method is carried out by searching and collecting data from literature sources obtained from several national and international journals that review bioactive compounds and the therapeutic potential of M. alba plants. Based on the literature review, it was found that M. alba has bioactive compounds namely linalool (72.8 flower oil and 80.1% leaf oil), α-terpineol (6.04% flower oil), phenylethyl alcohol (2.58% flower oil), β-pinene (2.39% flower oil) and geraniol (1, 23%) which have therapeutic activities such as mental health disorders, anticancer, anti-inflammatory, antihypertensive, antidiabetic, antihyperlipidemia, antimaagic, antidiarrheal, anti-asthma, and antihyperpigmentation. In traditional medicine it is used to treat fever, syphilis, gonorrhea and malaria, bronchitis, prostatitis, cancer, headache, sinusitis, cough, inflammation of the respiratory tract, chest fullness, flatulence, nausea, body and underarm odor, and vaginal discharge.

Prevalence of olfactory reference disorder according to the ICD-11 in a German university student sample

Olfactory reference disorder (ORD) is included as a new diagnosis in the 11th revision of the International Classification of Diseases (ICD-11). Its core symptom is persistent preoccupation with the belief that one is emitting a foul or offensive body odor or breath that is usually not perceptible to others. However, there is a lack of knowledge about the prevalence of ORD. In particular, the prevalence of the disorder has not yet been investigated using the ICD-11 diagnostic features. Therefore, this study aimed to assess the point prevalence of ORD according to the ICD-11. A total of 275 German students at university or college, aged 18–53 years, participated in an anonymous internet-based survey assessing ICD-11 essential features for ORD. The prevalence of ORD was 5.5%. Participants who met the self-rated features for ORD reported suicidal thoughts significantly more frequent than participants who did not meet the features for ORD. More than half of the participants with probable ORD reported ideas of reference and at least a strong conviction of their beliefs, suggesting poor insight. In the total sample, single symptoms of ORD were frequent. Persistent preoccupation with the belief of malodor was the most common single symptom. Distress and impairment linked to ORD symptoms were frequent. Our results suggest that ORD may be relatively common in university students and highlight the importance of future studies investigating the epidemiology of ORD as well as its suitable treatment.

Knockout of OR39 reveals redundancy in the olfactory pathway regulating the acquisition of host seeking in Anopheles coluzzii.

The attraction of anthropophilic mosquitoes to human host cues, such as body odour and carbon dioxide, gradually increases during adult maturation. This acquisition of host-seeking behaviour correlates with age-dependent changes in odorant receptor (OR) transcript abundance and sensitivity of olfactory sensory neurons (OSNs). One OR gene of the human malaria vector, Anopheles coluzzii, AcolOR39, is significantly downregulated in mature females, and a cognate ligand of AcolOR39, sulcatone, a major component of human emanations, mediates the observed behavioural inhibition of newly emerged (teneral) females to human body odour. Knockout of AcolOR39, using CRISPR-Cas9 mutagenesis, selectively abolished sulcatone detection in OSNs, housed in trichoid sensilla. However, knockout of AcolOR39 altered neither the response rate nor the flight behaviour of teneral females in a wind tunnel, indicating the involvement of other genes, and thus a redundancy, in regulating the acquisition of host seeking in mosquitoes.

Investigating Inattentional Blindness Through the Lens of Fear Chemosignals.

Inattentional blindness is a phenomenon wherein people fail to perceive obvious stimuli within their vision, sometimes leading to dramatic consequences. Research on the effects of fear chemosignals suggests that they facilitate receivers' sensory acquisition. We aimed to examine the interplay between these phenomena, investigating whether exposure to fear chemosignals (vs. rest body odors) can reduce the inattentional-blindness handicap. Utilizing a virtual-reality aquarium, we asked participants to count how many morsels a school of fish consumed while two unexpected stimuli swam by. We predicted that participants exposed to fear chemosignals (N = 131) would detect unexpected stimuli significantly more often than participants exposed to rest body odors (N = 125). All participants were adult Portuguese university students aged 18 to 40 years. The results confirmed our hypothesis, χ2(1) = 6.10, p = .014, revealing that exposure to fear chemosignals significantly increased the detection of unexpected stimuli by about 10%. The implications of our findings open a novel avenue for reducing the adverse consequences of inattentional blindness.

Air quality in the car: How CO2 and body odor affect drivers' cognition and driving performance?

Elevated indoor levels of CO2 and the presence of body odor have been shown to have adverse effects on the cognitive function of building occupants. These factors may also contribute to impaired in-car driving performance, potentially posing a threat to transportation and public safety. To investigate the effects of CO2 and body odor on driving performance, we enrolled 25 participants in highway driving tasks under three indoor CO2 levels (800, 1800, and 3500 ppm) and two body odor conditions (presence and absence). CO2 was injected in the cabin to increase CO2 levels. In addition, we assessed working memory and reaction time using N-back tasks during driving. We found that driving speed, acceleration, and lateral control were not significantly affected by either CO2 or body odor. We observed no significant differences in sleepiness or emotion under varying CO2 or body odor conditions, except for a lower level of emotion valence with exposure to body odor. Task load was also not significantly impacted by CO2 or body odor levels, except for a higher reported effort at 1800 ppm compared to 800 ppm CO2. However, participants did demonstrate significantly higher accuracy with increased body odor exposure, suggesting a complex effect of volatile organic compounds on driver cognition. Our findings also revealed moderating effects of task difficulty of N-back tests and exposure duration on cognition and driving performance. This is one of the first few in-depth studies regarding environmental factors and their effect on drivers' cognition and driving performance, and these results provide valuable insights for car-cabin environmental design for air quality and driving safety.

Embodying Otherwise

Larissa Lai’s (2002) visionary novel, Salt Fish Girl , is teeming with sensations beneath and beyond capitalist control. An atemporal narrative follows Miranda, a near-future young woman conceived by a durian and afflicted by a “memory disease” that produces distinctive body odor and flashbacks to episodes of colonial violence that the powerful would prefer to forget. While subjected to treatment for this “disease,” Miranda falls in love with Evie, erstwhile sweatshop laborer with carp DNA and body odor to match. In a story of anti-state, anti-capitalist rebellion, Miranda and Evie defect from straight, sane, and abled sterility. Aided by human/carp comrades called The Sonias, they realize spaces Elsewhere and Otherwise at the edges of the collective imagination. Using a queercrip conceptual framework, the article attends to the alienated, nonhuman body as a site of erotic play through Leah Lakshmi Piepzna-Samarasinha and Ellery Russian’s (2010) concept of the “crip lust of recognition,” arguing that it is within that space of affirmation and lust that criptopias beyond the “rational” might be found(ed). Finally, Salt Fish Girl is read alongside recent works of speculative activist-scholarship to theorize queercrip, multispecies resistance and imagination toward criptopian possibilities.

Unraveling the potential of breath and sweat VOC capture devices for human disease detection: a systematic-like review of canine olfaction and GC-MS analysis.

The development of disease screening methods using biomedical detection dogs relies on the collection and analysis of body odors, particularly volatile organic compounds (VOCs) present in body fluids. To capture and analyze odors produced by the human body, numerous protocols and materials are used in forensics or medical studies. This paper provides an overview of sampling devices used to collect VOCs from sweat and exhaled air, for medical diagnostic purposes using canine olfaction and/or Gas Chromatography-Mass spectrometry (GC-MS). Canine olfaction and GC-MS are regarded as complementary tools, holding immense promise for detecting cancers and infectious diseases. However, existing literature lacks guidelines for selecting materials suitable for both canine olfaction and GC-MS. Hence, this review aims to address this gap and pave the way for efficient body odor sampling materials. The first section of the paper describes the materials utilized in training sniffing dogs, while the second section delves into the details of sampling devices and extraction techniques employed for exhaled air and sweat analysis using GC-MS. Finally, the paper proposes the development of an ideal sampling device tailored for detection purposes in the field of odorology. By bridging the knowledge gap, this study seeks to advance disease detection methodologies, harnessing the unique abilities of both dogs and GC-MS analysis in biomedical research.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN JELANTIR (Conyza sumatrensis (Retz.) E. Walker) DALAM SEDIAAN DEODORAN SPRAY TERHADAP BAKTERI Staphylococcus epidermidis

The bacteria Staphylococcus epidermidis is known to cause body odour in the human body. A common method to kill bacteria that cause body odour is deodorant. Commercial deodorants contain aluminium chlorohydrate and the compound is known to increase the risk of skin cancer and alzheimer's disease. A candidate for a safer deodorant is one made from natural ingredients. One of the natural ingredients that has potential as a deodorant is jelantir leaves. The purpose of this study was to extract and characterise phytochemical compounds and test their antibacterial activity in deodorant spray preparations on Staphylococcus epidermidis. The extraction method used was maceration using ethanol solvent, extract characterisation using UV-Vis spectrophotometry and antibacterial test of deodorant spray preparation of jelantir leaf extract using disc diffusion. The results showed that jelantir leaf extract contained flavonoids 5.5727 ±0.1336 (%w/w QE), saponins 8.6472 ±0.0353 (% w/w SE), tannins 2.1448 ±0.0506 (%w/w TAE), steroids 1.0231 ±0.0083 (%w/w BSE), triterpenoids 0.9090 ±0.0030 (%w/w UAE) and the antibacterial test results had the highest inhibition zone diameter of 13.50 ±0.50 (mm) in formula 30 (%w/v).

FRI193 Early Presentation Of Pediatric Oncocytic Adrenal Tumor Associated With Germline TP53 Pathogenic Variant—Report Of Three Cases

Abstract Disclosure: R.R. Oppenheimer: None. L.P. Lins: None. C.P. Oliveira: None. I.P. Magalhaes: None. A. Latronico: None. B.B. Mendonca: None. M.Q. Almeida: None. A.W. Kuhn: None. H. Charchar: None. M.C. Fragoso: None. Oncocytic adrenal tumors (OAT) are uncommon and usually nonfunctional. In the pediatric age group, OAT are extremely rare and less than 20 cases have been reported. Herein we report the clinical presentation and follow up of 3 non-related children with Li-Fraumeni syndrome with a germline TP53 pathogenic variant (PV) (p.R337H) who developed secreting OAT. Case 1. A 19 month old male presented with moon face, weight gain, irritability and pubic hair (Tanner stage P3) for over 4 months. He had no family history of neoplasms and his parents were unrelated to each other. Hormonal data revealed serum cortisol 33 mcg/dL with a suppressed ACTH. Testosterone was elevated (242 ng/dL - RR &amp;lt;12 ng/dL) with low plasma gonadotropins. Androstenedione 3,08 (RR&amp;lt;0,5ng/mL). Abdominal CT revealed a left adrenal tumor (5.0 x 4.4 x 4.0cm). He underwent a left laparoscopic adrenalectomy and histopathologic analysis confirmed OAT Wieneke score 2/9. Immunohistochemistry was positive for inhibin, p53 and Ki67 (10-15%). The postoperative clinical course was uneventful. The TP53 p.R337H PV was inherited from his father. He is now prepubertal and there is no evidence of relapse in 14 months of monitoring. Case 2. An 18 month old female presented with acne, clitoromegaly, pubic hair (Tanner stage 2), axillary odor, axillary hair and weight gain. Hormonal study showed elevated total testosterone of 624.7 ng/dL, androstenedione, DHEA-S and prepubertal gonadotropins. 17OHP and cortisol were normal. Abdominal CT confirmed a heterogeneous solid nodule of 5.5 x 4.5 x 3.7 cm in the right adrenal topography. The patient underwent a right adrenalectomy and OAT was identified by histopathology (Wieneke score 3/9). Immunohistochemistry was positive for p53, inhibin and Ki67 (30%). The TP53 p.R337H PV was inherited from her mother. She had remission of secondary sexual characteristics and he is now prepubertal. Case 3. A 2 years and 8 months old girl was referred with precocious pubarche and clitoromegaly (2,6cm). She had a right flank palpable mass and tanner 1 breasts. Hormonal studies showed elevated testosterone (70ng/dL), androstenedione (3.81ng/mL) and DHEA-S (13490ng/mL), with LH 0.3IU/L and FSH 3.4IU/L. The cortisol after DST was 5.8mcg/dL and ACTH was suppressed. Abdominal MRI revealed a 5 cm tumor at the right adrenal gland. She underwent laparoscopic adrenalectomy. The histopathologic analysis revealed an OAT Wieneke score 1/9. Immunohistochemistry was positive for p53 and Ki67 (13%). The TP53 p.R337H PV was inherited from her father. Conclusion: Although rare, functional OATs have been reported in children. Herein we describe the first patients with the TP53 p.R337H PV to present with OATs. These are the youngest cases of OAT published so far. The clinical presentation, hormonal data and follow-up were not different from those of other pediatric adrenal tumors. So far there is no evidence of disease relapse during follow-up. Presentation: Friday, June 16, 2023

THU227 Isosexual Peudo-precocious Puberty Due To Leydig Cell Tumor In A Prepubertal Child

Abstract Disclosure: M. Baby: None. A. Xu: None. N. Shacham: None. E. Shapiro: None. B.C. Shah: None. Background: Testicular tumors account for 2-4% of all childhood cancers. Leydig cell tumors are the most common form of sex cord stromal tumors and represent 1-3% of all testicular tumors. In the pediatric population, testicular Leydig cell tumors present with signs of precocious puberty. Workup reveals low gonadotropins with elevated testosterone and testicular mass on ultrasound. Treatment involves surgical enucleation (partial orchiectomy) in most cases. Clinical case: We present the case of a 4 year 10 month old male who presented to endocrine clinic for precocious puberty. He developed pubic hair three months prior with body odor. He did not have any CNS symptoms including headaches or visual changes. There was no use of exogenous androgens at home. He was born full term, small for gestational age with an uncomplicated postnatal course. There was no family history of precocious puberty. Height was at the 99th percentile (z-score 2.7), weight 98th percentile (z-score 2.07), and BMI 86th percentile (z-score 1.09). On exam, he was lean and appeared muscular. His testes were asymmetrical with the right testicle measuring 3 ml and the left testicle 2 ml. Scrotal hair was curly and sparse, Tanner III. Stretched penile length was 7 cm (90th percentile), width was 3.2 cm. Results: Bone age was advanced to 10 year 0 months with height age of 7 year 3 months. Serum gonadotropins were low, testosterone was pubertal at 115 ng/dl (Electrochemiluminescence Immunoassay). Serum 17-hydroxyprogesterone (221 ng/dl, ref range 0-90 ng/dl) androstenedione (163 ng/dl, ref range = &amp;lt; 51 ng/dl), and IGF-1 (368 ng/ml, ref range 108 – 293 ng/ml) were elevated. ACTH, cortisol, thyroid profile, and prolactin were normal. Beta-HCG and AFP were negative. ACTH stimulation test showed normal response. Testicular ultrasound with doppler was performed; right testicle measured 1.82 x 1.06 x 1.36 cm, volume of 1.4 ml, while left measured 1.27 x 0.72 x 0.87 cm, 0.4 ml. Within the right testicle, there was a mass that was hypoechoic, vascular (with a testicular artery feeding branch in the periphery), multinodular and measured 1.2 x 0.8 x 1.10 cm (0.53 ml). He was referred to urology and underwent enucleation of the right testicular mass through an inguinal approach. Grossly, the mass appeared tan and lobulated and surgical pathology confirmed a benign Leydig cell tumor. The patient recovered well and post-operative serum testosterone level at 2 weeks was &amp;lt;2.5 ng/dl. Conclusion: We present a case of a testicular Leydig cell tumor that presented as peripheral precocious puberty marked by low gonadotropins and elevated testosterone with ultrasound findings of a vascular mass with testicular artery feeding branch in the periphery. Early detection of a Leydig cell tumor is crucial as it can be treated by enucleation thus salvaging the testes. Presentation: Thursday, June 15, 2023

FRI490 Association Between TSH Levels And Early Development Of Secondary Sexual Characteristics Among Children With Congenital Hypothyroidism

Abstract Disclosure: M.M. Nunez Stosic: None. T. Seeherunvong: None. Although hypothyroidism typically delays puberty, early pubertal development in patients with hypothyroidism and markedly elevated TSH levels has been reported. Recent studies investigated the association between thyroid function and timing of puberty among normal children. Jung et al. reported an association between serum TSH and LH levels in girls with central precocious puberty. By contrast Wilken et al. found an association between serum T4 level and onset of pubarche but not thelarche among female subjects. We investigated the association between serum TSH levels and onset of secondary sexual characteristics among patients with congenital hypothyroidism. A retrospective study was conducted including girls and boys aged ≥6 years and ≤ 18 years with confirmed primary congenital hypothyroidism who were seen in our pediatric endocrinology clinic at University of Miami, between May 2012 and May 2022. Age of onset of secondary sexual characteristics (SSC) such as body odor, acne, axillary hair, pubic hair, breast/testicular development was recorded. Early onset of SSC was defined by its presence in girls younger than 8 years and in boys younger than 9 years. Onset of SSC was considered early normal if it occurred between 8-9.5 years in girls and between 9-10.5 years in boys. A third group of patients were those with onset of SSC after 9.5 years in girls and after 10.5 years in boys. We recorded the serum TSH levels from age 6-8 years in girls and from 7-9 years in boys. We determined the average TSH levels and the proportion of the serum TSH level above normal range. IBM SPSS Statistics software version 28.0.0.0(190) was used for data analysis. Fifty-seven subjects with a history of congenital hypothyroidism, 36 girls (63.2%) and 21 boys (36.8%) were included in the study. Seventeen patients (29.8%) had early or early normal onset of SSC. The prevalence was more frequent among girls than boys, 14% and 3% respectively (p value= 0.046). Among all 57 subjects, only 9 had normal serum TSH. The mean proportion of TSH levels above normal limit was 62.4% in patients with early or normal early onset of SSC. By contrast, the mean proportion of serum TSH level above normal limit was 53.7% in those patients who had neither early nor early normal onset of SSC (p value=0.044). In summary, early and early normal onset of SSC was associated with higher TSH levels in children with congenital hypothyroidism. Further studies with a larger number of subjects will be needed to define further the specific SSC most associated with an abnormally elevated TSH. Presentation: Friday, June 16, 2023

Human scent as a first-line defense against disease

Individuals may have a different body odor, when they are sick compared to healthy. In the non-human animal literature, olfactory cues have been shown to predict avoidance of sick individuals. We tested whether the mere experimental activation of the innate immune system in healthy human individuals can make an individuals’ body odor be perceived as more aversive (intense, unpleasant, and disgusting). Following an endotoxin injection (lipopolysaccharide; 0.6 ng/kg) that creates a transient systemic inflammation, individuals smelled more unpleasant compared to a placebo group (saline injection). Behavioral and chemical analyses of the body odor samples suggest that the volatile components of samples from “sick” individuals changed qualitatively rather than quantitatively. Our findings support the hypothesis that odor cues of inflammation in axillary sweat are detectable just a few hours after experimental activation of the innate immune system. As such, they may trigger behavioral avoidance, hence constituting a first line of defense against pathogens of infected conspecifics.

Immunoactivation Affects Perceived Body Odor and Facial but Not Vocal Attractiveness.

Several previous studies have shown that in mammals, the health status of conspecifics can be assessed based on perceptual cues. Olfactory, visual, or acoustic cues may lead to avoidant behavior, thus reducing the risk of contagion by close contact with infected individuals. We tested whether immune system activation after immunization leads to perceptible changes in body odor and facial and vocal attractiveness in humans. We have experimentally activated the immune system of male participants using vaccination against hepatitis A/B and meningococcus. Their body odor, facial photographs, and vocal recordings were collected before and 14 days after vaccination. Subsequently, the body odor samples, facial photographs, and vocal recordings were assessed by female raters for their attractiveness and healthiness. We have also measured skin coloration (from facial photographs and in vivo using a spectrophotometer), vocal parameters, and C-reactive protein (CRP) levels as a marker of inflammation. We found an increase in perceived body odor attractiveness, a decrease in facial attractiveness and healthiness, and no change in vocal attractiveness 14 days after vaccination compared to the prevaccination condition. Moreover, there was no change in facial coloration or vocal parameters between the prevaccination and postvaccination conditions. Prevaccination CRP levels were negatively associated with body odor and facial attractiveness and positively associated with body odor intensity. Overall, our results suggest that perceived body odor as well as facial but not vocal attractiveness may provide cues to activation of the immune response and that each modality may carry specific information about the individual's condition.

Generalized Demodicosis and Malasseziosis in a Pug Mix Dog

A 12-year-old female pug mix dog presented a skin problem with itching, and alopecia in the neck, facial, and extremity areas. Findings of scales, hyperpigmentation, and erythema are present almost throughout the body, along with crusts on the cranial and caudal extremities and a foul body odor. Wood's lamp examination revealed a yellowish-green fluorescence in the ear and ventral areas of the body. The skin tape examination revealed an infestation of Demodex sp. mites accompanied by Malassezia sp. hematological analysis indicated that the dog presented anemia. The dog was diagnosed with demodicosis with a secondary infection of Malassezia sp. The therapy provided consisted of antiparasitic drugs such as imidacloprid and moxidectin (Advocate®); the long-acting antibiotic enrofloxacin was administered at a dose of 200 mg intramuscularly, along with the oral antibiotic amoxicillin and clavulanic acid at a dose of 31.25mg/kg BW every 12 hours. Additionally, oral antifungal medication ketoconazole was given at 25mg/kg BW every 12 hours, along with ketoconazole cream. Oral antihistamine mebhydroline napsylate was administered at 25mg/kg BW every 12 hours. Furthermore, multivitamins and hair vitamins were given at a dose of 2 ml once every 24 hours. After seven days of treatment, an evaluation was conducted, and it was observed that the dog's condition had improved, indicated by a reduction in the intensity of scratching.