Electrical percolation and piezo-resistivity under cyclic load were studied for silver nanoparticle (AgNP)/polydimethylsiloxane (PDMS) films. Attention was focused on the percolation process and the impacts of the percolation stage and load frequency on the piezoresistivity of the films. A power law was uncovered between the electrical resistance and the surface mass density (md) of AgNP layer. Dependence of piezoresistive sensitivity and linearity on md and cyclic load frequency were also investigated and interpreted in terms of the physical changes of the AgNP layer and the dynamics of the strain-induced microcracks. In particular, a peak gauge factor 75 was achieved at md = 0.32 mg cm−2 and the load frequency 0.5 Hz, and excellent linearity was found at the frequency 1 Hz or higher. These new findings provide important guidance for the design and applications of AgNP/PDMS films in body motion tracking, fatigue load monitoring and surface vibration detecting.