Background: Obesity (OB) is highly prevalent in postmenopausal women; particularly increased abdominal visceral adipose tissue contributes to endothelial dysfunction and cardiovascular risk. The endothelium plays an important role on blood flow (BF) and pressure control at rest and during exercise. However, it is unknown how different body mass index (BMI) categories influence upper and lower limb endothelial function and BF responses to exercise in postmenopausal women. Purpose: The purpose was to examine upper and lower limb endothelial function at rest and BF regulation during low-intensity exercises in OB, overweight (OW), and lean (LN) postmenopausal women. We hypothesized that endothelial function and BF responses to submaximal exercise in both limbs will be attenuated in OB compared to the OW and LN groups. Methods: Women were classified as OB (30.0-39.9 kg/m2; n = 13), OW (25.0-29.9 kg/m2; n = 15), and LN (18.5-24.9 kg/m2; n = 11) by BMI. Flow-mediated dilation (FMD) of the brachial (BA-FMD) and superficial femoral arteries (FA-FMD) were measured using ultrasound. BF and vascular conductance (VC) in the brachial (BA-BF and BA-VC) and femoral (FA-BF and FA-VC) arteries were measured during separate 3-stage incremental rhythmic handgrip and plantarflexion exercises (PFE) at 5, 15, and 30 % of maximal strength and calculated 1-repetition max (1RM), respectively. Results: No differences in BA-FMD were observed among the groups. FA-FMD was significantly lower in OB (2.6 ± 1.4%) compared to OW (4.3 ± 1.9%) and LN (4.2 ± 0.7%) groups (P < 0.05). Increases in FA-BF (OB: 462 ± 229 vs. OW: 616 ± 173 vs. LN: 640 ± 141 mL/min; P < 0.001) and FA-VC (OB: 410 ± 176 vs. OW: 585 ± 155 vs LN: 623 ± 153 mL/min*100mmHg; P < 0.001) were attenuated during PFE at 30% of 1RM in OB compared to OW and LN. Upper-body exercise vasodilation was not attenuated by OB. FA-BF and FA-VC during PFE at 30% of 1RM were correlated with FA-FMD (FA-BF: r = 0.423, P = 0.007, FA-VC: r = 0.367, P = 0.021) and BMI (FA-BF: r = -.386, P = 0.015, FA-VC: r = -.456, P = 0.004). Conclusion: Postmenopausal women with OB have blunted leg endothelial function and exercise vasodilator function during submaximal dynamic plantarflexion exercise compared to LN and OW. These findings suggest that OB may contribute to reduce leg endothelial function, and vasodilator function during exercise in postmenopausal women. No support of funding information to disclose. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.