Body Fat Research Articles

Evaluation of the Impact of Functional Hypogonadism in Body Composition and Weight Loss in Men Undergoing Bariatric Surgery.

Obesity and hypogonadism are linked in a vicious cycle: low testosterone levels favor weight gain and adiposity induces hypogonadism. We aimed to investigate if low levels of testosterone in pre-operative of bariatric surgery impacts postoperative weight loss (WL) and body composition (BC). A prospective, observational study included male patients who qualified for bariatric surgery. Patients underwent clinical evaluation, hormonal evaluation and assessment of body composition measured by dual energy X-ray absorptiometry in both pre-operative and postoperative periods. We evaluated 36 patients, mean age 37.1±10.2 years, weight 131.3±14.3 kg and BMI 44.4±4.95 kg/m2. Considering total testosterone (TT) <264 mg/dL and free testosterone <6.5 mg/dL (when TT was borderline), hypogonadism was found in 18 patients (50%). Among the 20 patients who underwent the total evaluation in the pre-operative period, the excess of weight was greater in hypogonadal men (58.1±1.,6 vs 51.1±13.5 kg) as well as the body fat percentage (BFP) (46.1±4.3 vs 45.1±4.5%), however, without statistical significance. After surgery, TT returned to normal in all hypogonadal patients, and patients showed similar WL and BFP. Regarding the relationship between visceral fat and testosterone, it was observed a moderate correlation between the change in TT levels and the reduction in visceral fat. Although frequently observed, low levels of TT in the pre-operative period do not impact post-surgical outcomes, in terms of WL or BC. The normalization of TT levels in the postoperative period is probably due to the gonadal function improvement provided by the WL after bariatric surgery.

Influence of puberty stage in immune-inflammatory parameters in well-trained adolescent soccer-players, following 8-weeks of pre-seasonal preparation training

Aim: The immune system can experience temporary suppression following acute or prolonged strenuous exercise, potentially increasing susceptibility to infections. Young athletes, who must balance school, training, studies, and social life, may further disrupt their immune-inflammatory responses. This study aimed to investigate the effects of an 8-week pre-season training on circulating leukocyte responses in well-trained adolescent soccer-players at different stages of puberty. Methods: Thirty-six soccer players, divided into two groups based on their biological age-under sixteen (U16) and above sixteen (A16)-underwent two rounds of assessments. These included evaluations of anthropometrics, physical fitness, and immune parameters before and after an 8-week pre-season soccer training program. Results: No significant treatment or interaction effects were found for erythrocyte sedimentation rate (ESR), platelets, total leucocyte count, immune inflammation markers (IIM), weekly rating of perceived exertion, body composition, sit-and-reach flexibility, or VO2max (P &gt; 0.05). However, post-intervention neutrophil counts were increased, while lymphocyte counts decreased, and IIM levels rose in both groups (P &lt; 0.05). Neuromuscular explosiveness and strength endurance were significantly higher in the A16 group compared to the U16 group (P &lt; 0.05). Body fat was reduced, and all physical fitness parameters improved in both groups over time (P &lt; 0.05). Conclusions: The 8-week soccer-specific training program did not lead to different effects on immune-inflammatory parameters between youth soccer players at different stages of puberty. However, training adaptations, including improvements in physical fitness, were observed in both groups. This suggests that when the same training load (frequency, duration, intensity) is applied to adolescents at different stages of puberty, immune-inflammatory responses are not significantly influenced by age, but physical fitness improves regardless of puberty stage. A key limitation of this study is the lack analysis of lymphocyte subpopulations, which could provide a deeper understanding of the cellular effects of training on immune function.

Obesity phenotype and gut microbiota alterations are not associated with anxiety-like behaviour in high-fat diet-fed mice.

Anxiety is a common co-morbidity with obesity and metabolic disease, and can lead to a significant impact on quality of life. The vast differences in the gut microbiota between obese and control individuals provide a potential avenue for therapeutic intervention. A high-fat diet (HFD) in rodent models have been shown to induce anxiety-like behaviour and has been tested through an array of distinct behavioural tests such as the elevated plus maze test, light-dark test and open field test. Despite differences in testing and assessment parameters, the behavioural outcomes have previously yielded similar results. Recent evidence suggests that HFD has an anxiolytic effect on mice, complicating the model. Here, we aimed to confirm whether HFD-fed mice are more susceptible to presenting anxiety-like behaviours. Our findings showed no significant differences in behaviour, plasma corticosterone and inflammation markers between HFD and control diet (CTD) mice, despite considerable differences in adiposity and faecal microbial communities. Additionally, daily oral gavage is one of the most common methods for testing bacterial probiotics in rodent models, but this handling could potentially also cause stress to the mice. Thus, we investigated if daily oral gavage could mask differences in HFD and CTD mice. We found no significant differences in weight, fat mass or anxiety-like behaviour in CTD-fed mice with or without daily oral gavage.

The Prevalence of Obesity is Increased in Adolescents with Amblyopia: An Analysis of National Health and Nutrition Examination Survey Data.

Background: Amblyopia is the most common cause of vision loss in children. Amblyopia has been associated with impaired depth perception but little attention has been paid to the extent to which amblyopia increases the risk of obesity. Methods: Public-use data from the 1999-2008 National Health and Nutrition Examination Survey were used. Analyses were limited to children aged 12-18, who had a visual examination, and a best corrected visual acuity (BCVA) of at least 20/40 in the better-seeing eye. Amblyopia was defined as two or more-line interocular difference in BCVA. Obesity was defined as Body Mass Index (BMI) or body fat percentage (BFP) ≥95th percentile for age and gender. Sedentary lifestyle was defined as cardiovascular fitness level (CFL) rating of "low." We used Mantel-Haenszel odds ratios (ORs) to examine the relative prevalence of obesity in children with/without amblyopia. Results: Adolescents with amblyopia (n = 360) were more likely than those without (n = 7935) to have a high BMI [OR = 1.56; 95% confidence interval (CI): 1.24-1.98; p < 0.001]. The associations with either high BFP (OR = 1.20; 95% CI: 0.86-1.56, p = 0.167) or low CFL (OR = 1.15; 95% CI: 0.83-1.57; p = 0.267) were not statistically significant but in the direction of a priori hypotheses. Conclusions: This analysis of population-based data suggests that adolescents with amblyopia may be at higher risk of having obesity. Given the high prevalence of amblyopia and the range of morbidities associated with childhood obesity, targeted interventions to reduce the risk of obesity among children with amblyopia could be warranted.

Sex-Specific Obesity Paradox in Critically Ill Patients With Severe Acute Kidney Injury: A Retrospective Analysis.

Although obesity is typically correlated with adverse outcomes in various diseases, certain acute critical illnesses exhibit a phenomenon known as the obesity paradox. This study evaluated sex-specific differences in the prognostic implications of the body mass index (BMI) of patients with severe acute kidney injury (AKI) requiring continuous renal replacement therapy (CRRT). A multicenter retrospective cohort study. Data from eight tertiary hospitals in Korea. A total of 3805 critically ill patients receiving CRRT. Patients were categorized into four BMI groups: underweight, normal weight, overweight, and obese. None. There were 2308 male and 1497 female patients. The 90-day mortality risk significantly differed among BMI groups in the overall patient population and the male subpopulation but not the female subpopulation. Following adjustment for confounding variables, the 90-day mortality risk was higher in the underweight group than in the obese group (hazard ratio [HR], 1.20; 95% CI, 1.05-1.36; p = 0.006). Among male patients, the 90-day mortality risk was higher in both the underweight and normal weight groups than in the obese group (underweight: HR, 1.30; 95% CI, 1.10-1.53; p = 0.002 and normal weight: HR, 1.18; 95% CI, 1.04-1.35; p = 0.010); however, no such association was observed among female patients. Subgroup analysis demonstrated the obesity paradox in male patients of old age, with septic AKI, or with low albumin levels. There were sex differences in the association between BMI and mortality in critically ill patients with severe AKI. Although the precise distribution of fat mass and muscle mass was not identified, obese male patients had a more favorable prognosis, which was not evident among female patients. These findings highlight the importance of considering sex-specific factors in understanding the complex relationship between obesity and mortality in critically ill patients with AKI.

Effects of Exercise and a Lifestyle App on Sleep-Disordered Breathing, Physical Health, and quality of Life

Background. Sleep-disordered breathing (SDB) is a growing public health problem, and non-invasive treatments may be a valid option. The objective of this randomized controlled trial was to assess the effects of a 12-week exercise program and a lifestyle app on SDB severity, physical health (anthropometry, body composition, physical fitness), and health-related quality of life (HRQoL) in 18–50 years old adults with mild-to-moderate SDB.Methods. One-hundred-ninety-two participants (age 37.4±6.3years, BMI 33.3±4.2kg·m−2, 52.6% males) with mild-to-moderate SDB (apnea-hypopnea index [AHI] ≥5&lt;30.0, objective snore ≥10%) were randomized into: Exercise (three times/week, 60 min), app, and control groups. The assessments included a three-night self-applied polysomnography, anthropometryt, body composition, physical fitness, and HRQoL. Repeated ANOVA, Wilcoxon signed-rank and Kruskal-Wallis test were used to evaluate the changes.Results. Time effect was observed in AHI (F=6.290, p=0.014), neck circumference (F=5.253, p=0.024), hand dynamometry (F=7.102, p=0.009), and 6-minute walking test (F=8.340, p=0.005). Group x time interactions were observed in weight (F=9.318, p=&lt;0.001), BMI (F=9.302, p=&lt;0.001), body fat percentage (F=4.756, p=0.01), body fat mass (F=5.916, p=0.004), and skeletal muscle mass (F=9.380, p=&lt;0.001). Intragroup changes were observed in physical functioning, vitality, and general health in both the exercise and app groups, in addition to role limitations due to physical health in the exercise group.Conclusions. The 12-week exercise program reduced AHI and increased skeletal muscle mass, physical fitness, and HRQoL in four domains. The lifestyle app program reduced weight, BMI, neck circumference, body fat, visceral adiposity and skeletal muscle mass while increased three HRQoL domains in participants with mild-to-moderate SDB.

Bone Health Determinants in Ambulant Prepubertal Boys With Duchenne Muscular Dystrophy Treated With Deflazacort: Findings From a 3-Year Study.

Duchenne muscular dystrophy (DMD) is complicated by bone fragility. This study aimed to elucidate changes in bone mineral density (BMD) and body composition over time and to explore associations with adiposity measures in DMD. A three-year follow-up analysis was performed of total body (TB) and lumbar spine (LS) dual-energy x-ray absorptiometry (DXA) measurements, anthropometric measures, Tanner stage and bone turnover biomarkers assessments, and the incidence of fragility fractures in 26 ambulant prepubertal DMD patients treated with deflazacort (DFZ). Age at baseline was 7.7 years (interquartile range: 6-9.2). The TB BMD Z-score declined over time and was negatively related to the TB fat mass percentage and fat mass index (p < 0.05), but not to body mass index (BMI) standard deviation score (SDS). In contrast LS bone mineral apparent density (BMAD) Z-score remained stable and normal. The cumulative incidence of fragility fractures was 19.2%; DMD boys with fractures displayed a 1.5-fold higher decline of TB BMD Z-score/year (p < 0.05) and a worse adiposity profile compared to fracture-free patients. No difference was found in DFZ dose or duration between the two groups. We observed a high incidence of fragility fractures, and identified fat tissue as a potential detrimental factor for bone health, suggesting a need for monitoring in DMD patients with excessive adiposity. Fat mass measures assessed by DXA could help to identify those at risk, enabling targeted interventions for better bone health. The co-occurrence of multiple glucocorticoid side effects might characterize patients at higher risk of fractures.

Acute Alpha-Glycerylphosphorylcholine Supplementation Enhances Cognitive Performance in Healthy Men.

Choline is an essential nutrient required for proper cell functioning. Due to its status as a precursor to acetylcholine, an important neurotransmitter connected to cognition and neuromuscular function, maintaining or enhancing choline levels is of interest. Supplementation with alpha-glycerylphosphorycholine (A-GPC) can maintain choline levels, but its ability to offer support towards cognition remains an area of ongoing research. Using a randomized, double-blind, placebo-controlled, crossover approach, 20 resistance-trained males (31.3 ± 11.0 years, 178.6 ± 7.3 cm, 84.6 ± 11.4 kg, 15.4 ± 5.6% body fat) consumed either a placebo (PL), 630 mg A-GPC (HD), or 315 mg (LD) A-GPC (GeniusPure®, NNB Nutrition, Nanjing, China). After resting hemodynamic assessments, participants took their assigned dose and had cognitive assessments (Stroop, N-Back, and Flanker), visual analog scales, and hemodynamics evaluated 60 min after ingestion. All participants then warmed up and completed vertical jumps and bench press throws before completing a bout of lower-body resistance exercise (6 × 10 repetitions using the Smith squat at a load of 70% 1RM). Venous blood was collected 5, 15, 30, and 60 min after completion of the squat protocol to evaluate changes in growth hormones, and follow-up visual analog scales and cognitive measurements were evaluated 30 min after completing the exercise bout. When compared to PL, changes in Stroop total score were statistically greater after HD (13.0 ± 8.2 vs. 5.2 ± 9.0, p = 0.013, d = 0.61) and LD (10.8 ± 7.7 vs. 5.2 ± 9.0, p = 0.046, d = 0.48) administration, in addition to significantly faster times to complete the Stroop test in the HD group when compared to PL (-0.12 ± 0.09 s vs. -0.05 ± 0.09 s, p = 0.021, d = 0.56). No significant differences between groups were found for the Flanker and N-Back assessments, while a tendency was observed for HD to have faster reaction times when compared to PL during the Flanker test. No group differences were realized for visual analog scales, physical performance, or growth hormone. Statistically significant changes in heart rate and blood pressure were observed in all groups, with all recorded values aligning with clinically accepted normative values. HD and LD A-GPC supplementation significantly increased cognitive performance in a group of young, healthy males as measured by changes in the Stroop Total Score and completion time of the Stroop test. These results offer unique insight into the potential for A-GPC to acutely increase cognition in a group of young, healthy males. While previous research has indicated potential for A-GPC to acutely improve cognition in clinical populations, extending these outcomes to healthy individuals can be potentially meaningful for a wide variety of populations such as athletes, race car drivers, military operators, and other non-athletic populations who desire and have a need to improve their mental performance. This study was retrospectively registered as NCT06690619 on clinicaltrials.gov.

Alternative Day Fasting protocol Attenuates High Fructose-Induced Activation of the TGF-beta/Smad Signaling Pathway

Purpose: This study aims to explore the protective effects of alternate-day fasting (ADF) against metabolic disturbances induced by high fructose (HF) intake, with a particular focus on modulating the transforming growth factor-beta1 (TGF-β1) / mother against decapentaplegic homolog 2 (Smad2) signaling pathway. Materials and Methods: Four groups of rats (n=7 per group) were included: Control, ADF, HF (20% fructose in drinking water), and HF+ADF. The ADF protocol was applied with 24 hours of ad libitum feeding followed by 24 hours of fasting over a 5-week period. After five weeks, body weight (BW), muscle, and fat mass were measured. Serum samples were analyzed using ELISA to assess levels of TGF-β1, Smad2, connective tissue growth factor (CTGF), and total oxidant-antioxidant status (TOS-TAS). Results: Results indicated that that HF significantly increased final BW, and ADF reduced this weight gain (p=0.001). ADF also led to lower gastrocnemius-soleus muscle weights compared to controls (p=0.001), but mitigated fructose-induced retroperitoneal fat accumulation. TAS levels were higher, and TOS levels were lower in the ADF groups, showing an antioxidant shift (p

Adipose tissue biology and effect of weight loss in women with lipedema.

Lipedema is a lipodystrophic disease that is typically characterized by a marked increase in lower-body subcutaneous adipose tissue that is purported to have increased inflammation and fibrosis, impaired microvascular/lymphatic circulation and to be resistant to reduction by weight loss therapy. However, these outcomes have not been adequately studied. We evaluated body composition, insulin sensitivity, metabolic health and adipose tissue biology in women with obesity and lipedema (Obese-LIP) before and after moderate (~9%) diet-induced weight loss. At baseline, people with Obese-LIP had ~23% greater leg fat mass, ~11% lower android-to-gynoid ratio and ~48% greater insulin sensitivity (all P<0.05) than women matched on age, BMI and whole-body adiposity. In Obese-LIP, macrophage content and expression of genes involved in inflammation and fibrosis were greater, whereas lymph/angiogenesis-related genes were lower in thigh than abdominal subcutaneous adipose tissue. Weight loss improved insulin sensitivity and decreased total fat mass, with similar relative reductions in abdominal and leg fat masses, but without changes in markers of inflammation and fibrosis. These results demonstrate that affected adipose tissue in women with lipedema is characterized by increased inflammation and fibrogenesis, and alterations in lymphatic and vascular biology. Moderate diet-induced weight loss improves metabolic function and decreases lower-body adipose tissue mass.

Associations between growth and childhood body composition in very preterm, late preterm and term children.

To determine how gestational age at birth and postnatal growth relate to body composition in childhood. We calculated conditional growth (birth-2 years, 2 years-6 years) and measured body composition at 2 and 6 years using bioelectrical impedance in cohorts of New Zealand children born very preterm (VPT; 23-31 weeks), late preterm (LPT; 35- <37 weeks) and term (≥37 weeks). We explored the relationships between growth and fat mass (FM) index and fat-free mass (FFM) index at 6 years using multivariable linear regression. Of 1125 children (51% male), 202 were VPT, 114 LPT and 809 Term. Compared to Term, VPT but not LPT were lighter and shorter at 2 and 6 years and had lower FM index and FFM index. The association between weight growth from 2 to 6 years and both FM index and FFM index at 6 years was stronger than for weight growth from birth to 2 years or height growth at any period in all gestational age groups. Size and body composition at 2 and 6 years are different between infants born VPT, but not LPT, and at term. Later weight growth is more strongly associated with childhood body composition than earlier growth.

Sleep profiles and their associations with adiposity and cardiorespiratory fitness among adolescents.

This study aimed to identify sleep profiles in a representative sample of Slovak adolescents and investigate their associations with adiposity indicators and cardiorespiratory fitness. Data from the 2022 Health Behaviour in School-aged Children (HBSC) study conducted in Slovakia were analysed. Survey questions on sleep duration and problems from the entire HBSC sample (n = 8906) were used to identify sleep profiles. Associations with adiposity indicators and cardiorespiratory fitness were investigated in a subsample of 924 adolescents (average age 13.3 ± 1.48; 56.2% boys) who completed the HBSC survey, bioimpedance analysis, and 20-metre shuttle run test. Three sleep profiles were identified-optimal sleepers, optimal sleepers with sporadic sleep problems and poor sleepers. Crude models showed that poor sleepers had significantly higher body fat percentage and fat mass index, along with lower cardiorespiratory fitness, compared to optimal sleepers. After adjustment, only the association between sleep profiles and cardiorespiratory fitness remained significant. The observed associations between sleep profiles and cardiorespiratory fitness may help better target future intervention resources towards adolescents with low cardiorespiratory fitness levels.

The Influence of Body Fat Percentage on Body Type Cognitive Bias Among Young Chinese Hospital Staff Members: A Cross-Sectional Study

Aims/Background This study expanded the existing literature on obesity and distortion of body image by examining subjective and objective body type among young medical workers, specifically investigating whether fat percentage independently influences body type cognitive bias. Methods We recruited 264 participants (41.29% male, mean age 26.2 ± 3.1 years, mean body mass index (BMI) 21.7 ± 3.1 kg/m2) at a comprehensive hospital in central China. The questionnaire responses of the enrolled participants concerning basic information and body type self-assessment were extracted. Their weights and body compositions were evaluated by the bioelectrical impedance analysis technique (BIA). Objective indicators included measured weight, BMI, fat percentage, waist circumference (WC) and waist-to-hip ratio (WHR). Results Based on this sample, the prevalence rate of body type cognitive bias was 28.79%. For women, the univariate regression analysis showed that fat percentage was significantly correlated with body type cognitive bias (odds ratio [OR] 1.107, 95% confidence interval [CI] 1.033–1.185, p = 0.004). BMI (OR 1.303, 95% CI 1.098–1.546, p = 0.002) and WC (OR 1.109, 95% CI 1.043–1.180, p = 0.001) might also be positively associated with body type cognitive bias. After adjusting for age, BMI, WHR, and job position, the risk of body type cognitive bias decreased with fat percentage up to the first inflection point (26.8%) (OR 0.78, 95% CI 0.62–0.98, p = 0.036), and then it increased up to the second inflection point (33.0%). When fat percentage exceeded 33.0%, the relationship lost statistical significance. No significant relationships were found for men. Conclusion Fat percentage is an independent, nonlinear factor influencing women's body type cognitive bias. Gender and fat percentage should be considered when establishing weight management intervention strategies to prevent obesity from becoming a public health problem.

Physical activity and cardiometabolic risk factors in sprint and jump-trained masters athletes, young athletes and non-physically active men

Abstract Objectives Assessing physical activity and cardiometabolic risk in masters athletes as an example of very high physical activity at old age. Methods Forty-three men were studied in full factorial design, either as sprint or jump-trained masters athletes (MA, n=10, age 60–75 years), as young sprint or jump-trained athletes (YA, n=10, age 20–35 years), older control participants (OC, n=11, age 60–75 years) or as young control participants (AC, n=12, age 20–35 years). We performed bio-electrical impedance analysis and assessed serum markers of lipids and glucose metabolism and C-reactive protein, structured training hours, and habitual activity via mobile actimetry. Results Body fat was greater in OC than in MA (23.9 [SD 4.2] % vs. 14.0 [SD 5.7] %, p&lt;0.001), and also greater than in YA and YC (both p&lt;0.001). Weekly training hours were comparable between MA and YA (7.9 [SD3.3] hours vs. 11.1 [SD 4.8] hours, p=0.69). Habitual walking distance was greater in MA than in OC (7,387 [SD 4,923] m/day vs. 4,110 [SD 1,772] m/day, p=0.039), and so was habitual running distance (667 [SD690] m/day vs. 132 [427] m/day, p&lt;0.001). HOMA-index was greater in OC than in MA (2.07 [SD 1.39] vs. 0.80 [SD 0.41], p=0.0039), and so was C-reactive protein (1.35 [SD 1.74] mg/l vs. 0.58 [SD 0.27] mg/ml, p=0.018), whereas serum lipids showed only moderate or no effect (all p between 0.036 and 0.07). Conclusions Improved body composition and physical activity levels in MA are associated with lower cardiometabolic risk, which seems more pronounced for insulin sensitivity and inflammaging than for lipid metabolism.

Key factors influencing cycling performance and overall race time in the Ironman 70.3 for amateur athletes

Abstract Purpose Previous study has shown that cycling is the most predictive modality in the Ironman 70.3 triathlon distance. As a result, understanding the physiological and anthropometric variables that are mostly closely related to cycling performance can help coaches and athletes to direct their training programs. This study aimed to investigate the physiological, anthropometric, and general training characteristics influencing overall race time and cycling split time in Ironman 70.3. The present study also investigated the significance of body composition as a performance-related variable. Methods A questionnaire was used to assess training characteristics in 12 athletes (six men and six women), body composition in dual X-ray absorptiometry, and physiological variables in an incremental cardiopulmonary test. Ironman 70.3 São Paulo–Brazil 2023 was completed by all participants. The relationship between performance and the variables measured were investigated, and a multiple regression model for cycling split time and overall race time was developed. Results Functional threshold power (FTP) can predict cycling split time in Ironman 70.3 (r2 = 0.638, p = 0.002). Maximal oxygen uptake ($$\dot{\text{V}}$$ V ˙ O2max) (r2 = 0.667, p = 0.001) can predict overall race time. FTP and $$\dot{\text{V}}$$ V ˙ O2max are also strongly related to lean mass and fat mass percentage. Conclusion While FTP is the most important predictor of cycling split time, $$\dot{\text{V}}$$ V ˙ O2max is the most important predictor of overall race time in an Ironman 70.3. Furthermore, because body composition (fat mass %) and muscle mass (kg) are variables strongly related to FTP and $$\dot{\text{V}}$$ V ˙ O2max, we recommend that coaches and athletes consider to conduct a body composition assessment.

Correlation between body fat percentage and aerobic capacity in various athletes

Introduction: The Body Fat Percentage is an indicator of the Body's fitness level and its effects on VO2max and thus the cardiovascular status of the athletes. Physical exercise can help to improve a healthy life interestingly. Aims: This study aims to understand the relationship between Body Fat Percentage and Aerobic Capacity of different athletes. Methods: A total of 150 athletes were randomly selected as the subjects for the present study. In the present study VO2max was measured by Step test in millimeters of oxygen per kilogram of body weight per minute (ml.kg-1.min-1) and Fat percentage was measured by Skinfold Calliper in Millimeters (mm). The collected data were inferential statistics and an independent t-test was applied to check the level of significance. The significance level was set at p &lt; .05. Descriptive and for the relationship appropriate multiple relation statistical tools will be used for the analysis of gathering data. Results: In between fat percentage and Step Test Pulse rate (b/min) coefficient of correlation (r = .441) and level of significance (p = .00) indicated statistical significance with a small positive correlation. At the time relationship between fat percentage and VO2max (ml/kg/min) (r = -.450) and level of significance (p = .00) indicated a statistically significant and medium negative correlation. Conclusion: The relationship between body fat percentage variables and VO2max was statistically significant and indicated a negative correlation but step pulse rate and fat percentage were statistically significant with a small positive correlation.

Clinical Protocol Effects With LED Photobiomodulation for Reducing Adipose Tissue in the Abdomen Region.

Reverse the fat accumulation and to improve body contouring with more safely than invasive procedures has become a necessary therapeutic approach, but little detailed when using LED photobiomodulation. To evaluate an application protocol with two different wavelengths (red and infrared LED) consecutively in the abdomen region alone or associated with the dermocosmetics application with lipolytic properties. Ninety patients with a significant amount of adipose tissue in the abdomen region were selected and randomized into three groups: Sham (SG), in which patients received a simulation of the treatment; LED (LG), in which participants received the application with LED; LED with dermocosmetic (LDG), in which the participants underwent a combination of LED and dermocosmetic treatment. The following assessments were carried out: anthropometric data (weight, height, perimetry, and adipometry); ultrasound examination to adipose layer; patient satisfaction questionnaire; histology of a donated a tissue sample. The main findings were a decrease in umbilical perimetry, a significant decrease in the body fat layer determined by ultrasound and significant histological changes that indicated an improvement in the appearance of the skin and an increase in the amount of macrophages in the subcutaneous layer. Histological data also showed an improvement in the appearance of the skin with an increase in collagen deposition and an increase in macrophages in the subcutaneous layer. The LED phototherapy application protocol, both alone and in association with the dermocosmetic, with the emission of two consecutive wavelengths, was effective in reducing the fat tissue in the abdomen region.

A Ketogenic Diet Followed by Gradual Carbohydrate Reintroduction Restores Menstrual Cycles in Women with Polycystic Ovary Syndrome with Oligomenorrhea Independent of Body Weight Loss: Results from a Single-Center, One-Arm, Pilot Study

Background/Objectives: Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of fertile age. Some studies suggest that a ketogenic diet (KD) may have a role in treating PCOS. We aimed to demonstrate the long-term effectiveness of a KD in PCOS. Methods: Eighteen patients with PCOS phenotype A were enrolled: 28% were of normal weight, 28% were overweight, and 44% had obesity. All participants followed a KD without meal replacements for 45 days. After this period, patients underwent gradual carbohydrate reintroduction over 45 days, and thereafter healthy eating indications were given. Twelve patients completed the study. The patients were assessed at baseline and after 6 months. Anthropometric data, body composition, pelvic ultrasound, blood chemistry, hirsutism, and menstrual cycles frequency were recorded; Results: Besides improvement in anthropometric parameters, menstrual cycles (p 0.012), ovarian volume (p 0.029), FSH (p 0.05), LH (p 0.037), and progesterone (p 0.017) improved independently of weight or fat loss. However, testosterone and hirsutism improvements were influenced by weight and fat mass reduction. Conclusions: Our study showed that a KD followed by gradual carbohydrate reintroduction in PCOS has beneficial effects medium term, mostly independent of body weight loss, even in normal-weight women, suggesting that nutritional ketosis exerts beneficial effects per se.

Preparation for Powerlifting Competition: A Case Study

This study assessed a male competitive powerlifter over a 6-month period that encompassed his pre-contest preparation and post-contest recovery. We conducted the following monthly assessments: multi-frequency bioelectrical impedance analysis (MF-BIA), B-mode ultrasound evaluation of muscle thickness (MT), body circumferences, handgrip strength, vertical jump height, isometric lower body strength, and questionnaires for perceived mood and sleep. The athlete undertook a 3-month pre-contest preparatory period that included an extreme energy-restricted diet intended to sufficiently reduce body mass. This period also included an 8-week pre-contest training regimen with a 10-day taper intended to promote peak strength increases in the target lifts for competition. During the pre-competition period, the athlete experienced rapid and substantial losses of body mass (10.3%), body fat (6.5%) and lean mass (3.3%), the majority of which was regained in the 2-month post-competition recovery phase. The athlete's isometric knee extension strength rose sharply during the initial month of the pre-competition phase (336.4 Nm), followed by consistent declines (299 to 262.2 to 283 Nm) over the next 3 months, eventually returning close to baseline values 2 months post- competition Jump height remained steady in the 3 months in the pre-contest phase but increased (+1.4 cm) in the 2 months post-competition while handgrip strength increased during the months preceding the competition (47.5 kg to 49 kg) but decreased to 42.3 cm post-competition. Overall, the athlete did not seem to have any marked sleep disturbances. The most notable changes in mood parameters were increased tension, anger, and depression immediately preceding the competition.

Single-cell transcriptome profiling of m6A regulator-mediated methylation modification patterns in elderly acute myeloid leukemia patients

Millions of people worldwide die of acute myeloid leukaemia (AML) each year. Although N6-methyladenosine (m6A) modification has been reported to regulate the pathogenicity of AML, the mechanisms by which m6A induces dysfunctional hematopoietic differentiation in elderly AML patients remain elusive. This study elucidates the mechanisms of the m6A landscape and the specific roles of m6A regulators in hematopoietic cells of elderly AML patients. Notably, fat mass and obesity-associated protein (FTO) was found to be upregulated in hematopoietic stem cells (HSCs), myeloid cells, and T-cells, where it inhibits their differentiation via the WNT signaling pathway. Additionally, elevated YT521-B homology domain family proteins 2 (YTHDF2) expression in erythrocytes was observed to negatively regulate differentiation through oxidative phosphorylation, resulting in leukocyte activation. Moreover, IGF2BP2 was significantly upregulated in myeloid cells, contributing to an aberrant chromosomal region and disrupted oxidative phosphorylation. m6A regulators were shown to induce abnormal cell-cell communication within hematopoietic cells, mediating ligand-receptor interactions across various cell types through the HMGB1-mediated pathway, thereby promoting AML progression. External validation was conducted using an independent single-cell RNA sequencing (scRNA-Seq) dataset. The THP-1 and MV411 cell lines were utilized to corroborate the m6A regulator profile; in vitro experiments involving short hairpin RNA (shRNA) targeting FTO demonstrated inhibition of cell proliferation, migration, and oxidative phosphorylation, alongside induction of cell cycle arrest and apoptosis. In summary, these findings suggest that the upregulation of m6A regulators in HSCs, erythrocytes, myeloid cells, and T-cells may contribute to the malignant differentiation observed in AML patients. This research provides novel insights into the pathogenesis of AML in elderly patients and identifies potential therapeutic targets.