Body Condition Research Articles

Risk factors affecting all-cause mortality in cats hospitalized by a referral soft tissue service.

The objective of this study was to describe the all-cause mortality rate in cats hospitalized by the soft tissue surgery service of an academic referral hospital over a 5-year period and to identify specific risk factors for mortality. The hypotheses were that the all-cause mortality rate during hospitalization would be low, and cats undergoing emergency surgery and those with an American Society of Anesthesiologists (ASA) status of 3 or more would be at increased risk for mortality. The case log of cats hospitalized by the soft tissue surgery service at the University of Georgia was searched retrospectively to identify all cats hospitalized in the years 2015-2020. Data collected about each cat included age, sex and neuter status, weight, body condition score (1-9), pre-existing heart disease, chronic kidney disease, concurrent infection or cancer, emergency status, time of surgery (daytime vs after hours, which was defined as after 4 pm), if the surgery was performed on a weekday or weekend, and general type of surgery. Univariable logistic regressions were implemented to test and estimate odds ratios for the effects of risk factors on in-hospital mortality. A multivariable logistic regression was developed that initially included all risk factors with P <0.05 on univariable analysis. Log-likelihood ratio test P values and profile-likelihood confidence intervals were reported. The all-cause mortality rate was 6.1%. Analysis was limited because of low mortality, but multivariable analysis identified increasing ASA status and emergency surgery as significant risk factors for increased mortality while hospitalized. The findings of this study confirmed that increasing ASA status and emergency procedures are significant risk factors for mortality in cats. Clinicians should be aware of these risk factors and consider how to best monitor and manage these feline patients.

Prevalence of gastrointestinal and blood parasites in horses of Nakhon Si Thammarat province, Thailand

Background and Aim: The global prevalence of gastrointestinal (GI) and blood parasite infections in horses is a significant concern due to their substantial impact on morbidity, mortality, and economic losses in the horse industry. In Thailand, limited research has been conducted on these parasites in horse populations, and data from southern Thailand are lacking. Consequently, this study aimed to estimate the prevalence of GI and blood parasites in horses in Nakhon Si Thammarat province, Thailand. Materials and Methods: In total, 79 fecal and blood samples were collected from horses across 11 farms in Nakhon Si Thammarat province. The fecal examination was conducted using simple flotation, formalin-ethyl acetate sedimentation, and a modified McMaster technique. Conventional polymerase chain reaction (PCR) was used to identify blood and strongyle parasites. The influence of sex, age, and body condition score on the prevalence of GI parasites was also analyzed. Results: Six GI parasites were detected: four nematodes (Oxyuris equi, Parascaris equorum, strongyles, and Strongyloides westeri), one trematode (Gastrodiscus aegyptiacus), and one protozoan (Eimeria leuckarti). The overall prevalence of GI parasites was 74.7%, with single strongyle infections accounting for the highest proportion at 50.6%, followed by co-infections of strongyles and G. aegyptiacus at 10.1%. All 11 pooled strongyle samples were positive for cyathostomins and Strongylus vulgaris using conventional PCR with specific primers. Sex was significantly associated with the overall prevalence of GI parasites, whereas both sex and age were significant risk factors for infection by strongyle parasites. Theileria equi was the only blood parasite species detected in the surveyed horses, with a prevalence of 1.3% (n = 1/79). Conclusion: This study is the first to estimate the prevalence of GI and blood parasites in horses from Nakhon Si Thammarat province, Thailand. These findings highlight the importance of implementing control measures against GI parasites and are pivotal for developing effective infection prevention strategies. Keywords: blood parasites, gastrointestinal parasites, horses, southern Thailand.

Apex carnivores coping with metal(loid) pollution and oxidative stress: biological and environmental drivers of variation in kidney of European brown bear

Induction of oxidative stress and the associated lipid peroxidation is a prevalent mechanism through which certain metal(loid)s exert nephrotoxic effects in mammals. Research on the toxic impacts of metal(loid)s in free-ranging large mammals at high trophic positions is exceedingly rare, yet crucial for understanding environmental exposure scenarios relevant to both human and animal risk assessment. Renal cortex tissues (N=457) of free-ranging brown bears (Ursus arctos) from the Dinara-Pindos population sampled in Croatia were analysed herein for toxic metal(loid)s and the underlying biological and environmental drivers of variation, with their time trend monitored during the 2009-2022 period. In 28 individuals from the 2021-2022 period, we additionally investigated associations between metal(loid)s and oxidative stress and damage biomarkers in renal cortex cells. The principal generalized linear models used to approximate variations in biomarkers of oxidative stress and damage included non-essential As, Cd, Pb, Tl and U, and essential Co, Cu, Fe and Zn. Age class and season of sampling had no impact on biomarker levels, except for lipid peroxidation, LP (April ↑). Age, sex (females ↑), body condition index (↓) and season of sampling significantly influenced metal(loid)s levels. Non-specific mammalian thresholds were crossed for Cd and Pb toxicity in 1-16% and 2% of population, respectively. Renal levels of metal(loid) did not exhibit a clear trend over the 13-year period. The levels found in this study were higher than in sympatric carnivorous and herbivorous species, but in line with findings in ursids worldwide. Potential adverse health effects from environmental exposure in brown bears may arise from disruption of oxidative balance, as evidence clearly indicated associated changes in catalase activity (↑), glutathione content (↑), LP (↓), reactive oxygen species (↓), total antioxidant capacity (↑) in the renal cortex due to the presence of the most toxicologically relevant Cd and Pb.

Use of real-time ultrasound measurements of fat thickness and longissimus thoracis muscle characteristics for predicting body fat depots in crossbred hair ewes

This study aimed to predict body fat depots using ultrasound measurements (USM) of fat thickness and longissimus thoracis muscle characteristics in crossbred hair ewes. A total of 24 animals with a mean body weight (BW) of 37 ± 4kg and a body condition score of 2.39 ± 0.49 were used. USM was recorded 24h before slaughter and included: subcutaneous fat thickness (SFT), area (LTMA), amplitude (LTA), and depth (LTD) of the l. thoracis muscle and kidney fat thickness (µKFT). After slaughter, the internal fat (IF) was separated, classified, and weighed as mesenteric (MF), omental (OF), or pelvic fat (PF). The left half was then separated into subcutaneous and intermuscular fat (CF), and the muscle and bone tissues were weighed separately and adjusted to take account of the whole animal. Total body fat (TBF) was determined to be the IF plus the CF weights. LTA and LTMA correlated poorly to moderately with fat depots (0.37 ≤ r ≤ 0.74, P < 0.05). Other than CF, µKFT showed poor to moderate correlation with the other depots of body fat (0.44 ≤ r ≤ 0.75, P < 0.05). The regression model used to predict MF had r2 of 0.87 (RSD=0.14kg) and included BW and LTMA (P<0,05). OF and PF were predicted with r2 of 0.55 and 0.44, respectively, including µKFT (RSD = 0.20 and 0.17kg). IF was predicted by empty BW, LTMA, and µKFT with r2 as 0.81 (RSD=0.30kg). For TBF, EBW and LTMA explained 72% of its variation (RSD= 0.59kg). USM of fat thickness and l. thoracis muscle characteristics can improve the accuracy of predicting body energy reserves in crossbred hair ewes.

Well-Being and Performance of Nursery Pigs Subjected to Different Commercial Vaccines Against Porcine Circovirus Type 2, Mycoplasma hyopneumoniae and Lawsonia intracellularis

Background/Objectives: Vaccination is a strategy in pig farming for the control of several pathogens, but commercial vaccines may have detrimental side effects. This study aimed to evaluate the effects of commercial vaccines on the control of porcine circovirus type 2 (PCV2), Mycoplasma hyopneumoniae (Mhp), and Lawsonia intracellularis (L. intracellularis) and their potential side effects on welfare, behavior, acute inflammation biomarkers (C-reactive protein and haptoglobin), and the performance of piglets during the nursery phase. Methods: A total of 240 piglets, both female and castrated males, with an average weight of 6.3 ± 0.9 kg were subjected to four treatments: T1-FLEXcombo® (Ingelvac®CircoFLEX and Ingelvac®MycoFLEX) + Enterisol® Ileitis; T2-FLEXCombo® + Porcilis® Ileitis; T3-Porcilis® PCV M HYO + Porcilis® Ileitis; and T4-FLEXCombo® + 0.9% saline solution. This study measured therapeutic interventions, body condition score, behavioral changes, rectal temperature, and inflammation biomarkers post-vaccination. Results: The T3 group required more therapeutic interventions and exhibited a 23.1% higher incidence of thin body condition (p &lt; 0.05) and 10 times more animals with depressed behavior than T1 (p &lt; 0.05). The piglets vaccinated for L. intracellularis (T2 and T3) had rectal temperatures exceeding 39.7 °C post-vaccination, significantly higher than in T1 (p &lt; 0.05). The T1 animals showed five times more positive behavior traits 24 h after vaccination (p &lt; 0.05). Touch response was 29% lower in the T2 and T3 groups, and the lying down behavior was higher in these groups compared to T1. Additionally, 41.7% of the T3 animals exhibited a sitting posture 48 h after vaccination. Higher serum C-reactive protein and haptoglobin levels were observed in T3 (p &lt; 0.05) at 24 and 48 h post-vaccination. Feed intake was higher in T1 compared to T3 between 29 and 35 days of age. It is important to note that this study did not measure immune responses to the pathogens and did not include challenge tests, and therefore, it does not assess which vaccine is superior in pathogen control. Conclusions: The vaccine programs resulted in similar zootechnical performance. However, T1, T2, and T4 showed better effects on piglet welfare and behavior compared to T3.

Impacts of winter crop choice and ewe body condition score change on whole farm profitability and production

Variations in body condition score (BCS) in ewes influences lamb survival, ewe survival and ewe lactation performance. Feeding a low protein diet such as fodder beet to ewes in mid-to-late pregnancy increases lamb losses and reduces lamb weaning weight even further. However, using high yielding forages such as fodder beet can reduce the area of winter crop required, introducing potential whole-farm benefits which may alter profitability. We analysed the effects of winter crop choice (brassica or fodder beet) and BCS (maintaining or reducing) on whole-farm production and profitability, using a targeted supplement to offset the impacts of fodder beet feeding. Eight farms were selected to represent low or high rainfall (590 and 1090 mm/annum respectively) regions of the South Island. Impacts of changing BCS on ewe and lamb survival at lambing, and lamb liveweight gain to weaning were calculated when fed either adequate (brassica) or low protein (fodder beet) diets in mid-to-late pregnancy. Production statistics and profitability were calculated using Farmax. Whole farm profitability was analysed using BCS change and winter forage crop as main treatments, blocked by low or high rainfall. Applying the effects of BCS and low protein diets on reproductive performance from prior published studies resulted in variation of 102 to 141 lambs weaned per 100 ewes mated, with the lowest value when the low protein, fodder beet only diet was used. Greatest number of lambs weaned was achieved when BCS was maintained through winter through provision of a balanced supplement, regardless of winter forage diet (P&lt;0.05). Lamb sale dates were later when fodder beet was used, due to lower lamb weaning weights, resulting in marginally higher prices due to changes in pricing schedule. Farm profitability was increased by 25% when BCS was maintained though winter (P&lt;0.05) irrespective of the diet used. Use of fodder beet must be accompanied by targeted supplementation to avoid ewe BCS loss increased lamb losses and the negative effects on wastage and animal welfare. Extra profitability could only be generated by opportunities to sell surplus feed during the summer. These responses were similar in both high and low rainfall environments. This study is unique in testing measured responses of BCS and targeted nutritional supplementation under specific physiological conditions on whole-farm profitability. Farmer decisions on feed utilisation and sale also have a significant influence on the potential to mitigate the effects of variations in winter forage choice.

Growth Promotion and Economic Benefits of the Probiotic Lactiplantibacillus plantarum in Calves

Objectives: Various measures have been attempted to prevent infectious diseases in calves, such as environmental improvement and vaccine administration. Probiotics are commonly used to improve the body condition of newborn calves and prevent disease. In our previous research, Lactiplantibacillus plantarum RGU-LP1 (LP1) suppressed the expression of inflammatory cytokines in PBMCs of cattle fed it in the diet. In this study, we evaluated the effect of LP1 on the weights and number of treatments of the calves. Methods: Twenty-six one-week-old Holstein bull calves were divided into two groups (thirteen each), the LP1 group (LP1-treated) and the CN group (no LP1 fed), and tested as follows. The LP1 group was fed lyophilized LP1 (109 CFU/head/day) in milk replacer for 40 days. The CN group was fed the same diet only. Calves were followed for 63 days. The average treatment costs for the LP1 during the period were recorded. Feces and blood were collected from each calf during this period. Feces were examined for gut microbiota, and blood for immune assay and cytokine gene expression. Results: The LP1-treated group showed a decrease in disease incidence and an increase in body weights compared to controls. The average treatment cost during the observation period was significantly reduced compared to the CN group. The expression of TGFβ and IL10, inhibitory cytokines of inflammation, was significantly increased. The simultaneous expression of this set of inhibitory molecules resulted in low serum IL1β levels during the growth period. Conclusions: The Th1-type cytokine IFNγ was also significantly increased in LP1-treated calves. By reducing the amount of disease treatments and increasing dairy gain, LP1 is effective in preventing infectious diseases in calves. In addition, the increase in IFNγ by LP1 indicates improved Th1-type immunity in calves. These results show that LP1 has effects on the regulated inflammatory response and growth of calves.

Impact of a two-stage rumen cannulation on the health and rumen function of six lactating dairy cows.

To determine the impact of a two-stage rumen cannulation on the health and rumen function of lactating dairy cows. Experimental study. Six lactating Holstein cows. We performed a two-stage rumen cannulation in six Holstein cows that were 49 ± 11 days in milk. The following clinical health parameters and digestion-associated variables were analyzed on seven measurement days, from before the first surgery until 28 days after the second surgery: body temperature, heart rate, respiratory rate, pain score, rumen fill score, fecal score, wet sieving, auscultation and palpation of the rumen, bodyweight, body condition score, and activity. The pain score of the cows was constantly zero. Similarly, the body temperature and respiratory rate remained within physiological ranges, whereas the heart rate was slightly higher immediately after the second surgery. No differences were observed in rumen fill (2.00-2.67; p = .10) and fecal consistency scores (2.17-2.67; p = .42). The fecal particle size distribution showed negligible differences. The cows lost approximately 43 kg of bodyweight during the experiment (p < .01), which was reflected in a 0.5-point body condition score loss (p < .01). A temporary minimal negative effect of a two-stage rumenostomy on the health and body condition of early lactating Holstein cows was observed, whereas digestion was unaffected. Considering the limited sample size, further studies are required to substantiate these findings. Given that animals are appropriately medically managed, experimental rumenostomy of lactating dairy cows may not compromise their health or rumen function.

Chronic toxic effects of chloroxylenol exposure on Rana chensinensis: Insights from endochondral ossfication

Chloroxylenol (para‑chloro-meta-xylenol, PCMX), is a widely used antimicrobial agent and can remain in the aquatic environment. Although toxicity studies related to PCMX on the aquatic animals like zebrafish and Brachionus koreanus have been reported, there are few reports in the ecological risk of amphibians. In this study, the toxicity of different concentration (143, 14.3, 1.43 μg/L) of PCMX treatments on the endochondral ossification and body condition of Rana chensiensis tadpoles was investigated at environmentally relevant concentrations during metamorphosis. The chronic exposure of PCMX decreased bone length and ossification of limbs, caused changes of thyroid gland structure and ossification related gene expression levels. Besides, we found that R. chensiensis developed rheumatoid arthritis. Therefore, these results provided valuable evidence that the ecological risk of PCMX that will negatively affect the body condition, thyroid hormones homeostasis and skeletal development of R. chensiensis tadpoles.

Bovine tuberculosis reactor cattle in Southwest Ethiopia: Risk factors for bovine tuberculosis

Bovine tuberculosis, caused by Mycobacterium bovis, is a chronic zoonotic disease that persists in Ethiopia despite global control efforts, impacting public health and the economy. However, little is known about the epidemiology of bovine tuberculosis in Southwest Ethiopia. This study aims to assess the prevalence of bovine tuberculosis and identify associated risk factors in dairy cattle. A cross-sectional study was conducted from October 2022 to October 2023 in four districts in the Sheka, Bench-Sheko, and Keffa zones. A multistage sampling approach was employed, resulting in the selection of 1,152 cattle from various herds. Tuberculin skin testing was performed to detect bovine tuberculosis, and data on potential risk factors were collected through questionnaires. Logistic regression models were used to analyze the association between bovine tuberculosis and various risk factors. The overall prevalence of bovine tuberculosis was 4.95% at the cow level and 22.19% at the herd level in the study areas. Significant herd-level risk factors for bovine tuberculosis included lowland areas, larger herd sizes, and the introducing new animals. At the cow level, risk factors included age, breed, body condition, herd size, introduction of new animals, and management system. The study reveals a high prevalence of bovine tuberculosis in dairy cattle in Southwest Ethiopia, identifying key risk factors. Effective control requires targeted interventions, such as improved management practices, enhanced farmer awareness, and a better understanding of and guidance on bovine tuberculosis strategies in Ethiopia. Future research should isolate and characterize M. bovis in the study areas.

Characterization of white blood cell ratios in South American camelids presented at a veterinary teaching hospital

White blood cell (WBC) ratios are used as diagnostic markers for various inflammatory or tumor diseases as well as stress in a broad range of species. The aim of this work was to provide data on five WBC ratios (neutrophil-to-lymphocyte ratio [NLR], band neutrophil-to-lymphocyte ratio [BLR], band neutrophil-to-neutrophil-to-lymphocyte ratio [BNLR], band neutrophil-to-neutrophil ratio [BNR] and lymphocyte-to-monocyte ratio [LMR]) in South American camelids (SAC) and characterize their association with demographic and important diagnostic parameters. Medical records of 307 SAC (275 alpacas, 32 llamas) that were presented at a veterinary teaching hospital were evaluated retrospectively. WBC ratios were calculated based on hematologic results of the initial blood samples. The influence of species, sex, age, body condition score, WBC count, and anemia on those ratios was investigated using descriptive statistics and generalized linear models. NLR, BLR and LMR were found to be significantly influenced by age and WBC count. Associations of individual WBC ratios with species, nutritional status or an anemic condition could be detected. NLR was 4.32; 2.31–7.81 (median; IQR), BLR 0.24; 0.07–0.87, BNLR 3.66 × 10–3; 1.17 × 10–3 − 14.20 × 10–3, BNR 0.06; 0.02–0.15 and LMR was 7; 3.54–14.67. Our data might serve as a basis for further studies on WBC ratios in SAC. The animals in this study showed a variety of underlying diseases. It should hence be noted that these values are orientation values and provide a representative overview of conditions in a clinic, but are not suitable as reference values for healthy animals.

Population changes in a Southern Ocean krill predator point towards regional Antarctic sea ice declines

While foraging, marine predators integrate information about the environment often across wide-ranging oceanic foraging grounds and reflect these in population parameters. One such species, the southern right whale (Eubalaena australis; SRW) has shown alterations to foraging behaviour, declines in body condition, and reduced reproductive rates after 2009 in the South African population. As capital breeders, these changes suggest decreased availability of their main prey at high-latitudes, Antarctic krill (Euphausia superba). This study analysed environmental factors affecting prey availability for this population over the past 40 years, finding a notable southward contraction in sea ice, a 15–30% decline in sea ice concentration, and a more than two-fold increase in primary production metrics after 2008. These environmental conditions are less supportive of Antarctic krill recruitment in known SRW foraging grounds. Additionally, marginal ice zone, sea ice concentration and two primary production metrics were determined to be either regionally significant or marginally significant predictors of calving interval length when analysed using a linear model. Findings highlight the vulnerability of recovering baleen whale populations to climate change and show how capital breeders serve as sentinels of ecosystem changes in regions that are difficult or costly to study.

Environmental factors regulating the population dynamics and reproductive strategy of freshwater mussel Parreysia corrugata in the Padma River, Bangladesh

Freshwater mussel Parreysia corrugata is an ecologically important species endemic to south-east Asia, which has been suffering from a dramatic decline in recent years. The present study investigated the population dynamics and reproductive strategy of P. corrugata in a changing climate of Bangladesh waters. Mussels were sampled monthly from January to December 2021 from the Padma River near the Rajshahi city of Bangladesh. A total of 809 specimens were collected with a sizes ranging from 36.07 to 101.41 mm shell length and 7.92–87.54 g weight, respectively. The mussels displayed a growth pattern characterized by negative allometry in body weight-shell length relationship. The asymptotic length (L∞) was 106.05 mm, the growth coefficient (K) was 0.370 per year, and the growth performance index (Φ′) was 3.58. The recruitment of P. corrugata was occurred throughout the year, with a peak during the month of February. The annual total mortality (Z) was 1.15, and natural mortality (M) and fishing mortality (F) were 0.61 and 0.54 per year, respectively. The exploitation level was E = 0.47 which indicates a state of under exploitation. Sex was identifiable throughout the study period and the sex ratio of the mussels did not differ significantly from the 1:1 (M: F) ratio ((χ2 = 15.80, P > 0.05). In terms of gonadal development, maturation, and spawning activity, both sexes had similar trends. Histology identified five gonad development stages for both male and female mussels: early developing, late developing, ripe, spawning, and spent. Monthly GSI data showed significant seasonal fluctuations with the highest GSI in April and it was sharply declined in May indicating the peak spawning of the mussels, which was in consistent with condition index and gonadal histology. All the physicochemical parameters showed significant variation except for pH. The most important factors explaining mussel body condition (b value) and reproductive strategies (GSI and CI) were water temperature, dissolved oxygen and water level. Although no known major threats of this species have been documented from Bangladesh, conservation measures could be adopted to protect the natural habitats of P. corrugata from adverse physicochemical and hydrological factors.

Genomic Prediction and Genome-Wide Association Studies for Productivity, Conformation and Heat Tolerance Traits in Tropical Smallholder Dairy Cows.

Genomic selection (GS) and genome-wide association studies (GWAS) have not been investigated in Vietnamese dairy cattle, even for basic milk production traits, largely due to the scarcity of individual phenotype recording in smallholder dairy farms (SDFs). This study aimed to estimate heritability (h2) and test the applicability of GS and GWAS for milk production, body conformation and novel heat tolerance traits using single test day phenotypic data. Thirty-two SDFs located in either the north (a lowland vs. a highland) or the south (a lowland vs. a highland) of Vietnam were each visited for an afternoon and the next morning to collect phenotype data of all lactating cows (n = 345). Tail hair from each cow was sampled for subsequent genotyping with a 50K SNP chip at that same visit. Milk production traits (single-test day) were milk yield (MILK, kg/cow/day), energy corrected milk yield adjusted for body weight (ECMbw, kg/100 kg BW/day), fat (mFA, %), protein (mPR, %) and dry matter (mDM, %). Conformation traits were body weight (BW, kg) and body condition score (BCS, 1 = thin to 5 = obese). Heat tolerance traits were panting score (PS, 0 = normal to 4.5 = extremely heat-stressed) and infrared temperatures (IRTs, °C) at 11 areas on the external body surface of the cow (inner vulval lip, outer vulval surface, inner tail base surface, ocular area, muzzle, armpit area, paralumbar fossa area, fore udder, rear udder, forehoof and hind hoof), assessed by an Infrared Camera. Univariate linear mixed models and a 10-fold cross-validation approach were applied for GS. Univariate single SNP mixed linear models were applied for the GWAS. Estimated h2 (using the genotype information to build relationships among animals) were moderate (0.20-0.37) for ECMbw, mFA, mPR, mRE, BW, BCS and IRT at rear udder; low (0.08-0.19) for PS and other IRTs; and very low (≤ 0.07) for MILK, ECM and mDM. Accuracy of genomic estimated breeding values (GEBVs) was low (≤ 0.12) for MILK, ECM, mDM and IRT at hind hoof; and moderate to high (0.32-0.46) for all other traits. The most significant regions on chromosomes (BTA) associated with milk production traits were 0.47-1.18 Mb on BTA14. Moderate to high h2 and moderate accuracies of GEBVs for mFA, mPR, ECMbw, BCS, BW, PS and IRTs at rear udder and outer vulval surface suggested that GS using single test day phenotypic data could be applied for these traits. However, a greater sample size is required to decrease the bias of GEBVs by GS and increase the power of detecting significant quantitative trait loci (QTLs) by GWAS.

Effects of Lactiplantibacillus plantarum CBT LP3 and Bifidobacterium breve CBT BR3 supplementation on weight loss and gut microbiota of overweight dogs

The prevalence of obesity in dogs is increasing worldwide. This study evaluated the effects of a mixed probiotic formula on the weight, body condition score (BCS), blood metabolite profiles, and gut microbiota of overweight and obese dogs over a 12-week supplementation period to determine the anti-obesity effects of Lactiplantibacillus plantarum CBT LP3 and Bifidobacterium breve CBT BR3. This was a community-based, randomized study that sampled 41 overweight and obese dogs with a veterinarian-determined BCS of 6 or more. The physical activity of all the subjects was measured using a pedometer designed exclusively for dogs. The food intake was measured using the developed application. Only the treatment group received the mixed probiotic formula twice daily (3 g per dose). A significant decrease in body weight (p < 0.0001), BCS (p < 0.0001), serum TG (p < 0.0001), serum TC (p = 0.0400), and serum leptin (p = 0.0252), and a significantly increased serum adiponectin levels (p = 0.0007) were observed in the treatment group compared with the values in the control group. Microbiota analysis showed that Lactiplantibacillus increased and Erysipelatoclostridium, Staphylococcus, and Gemella decreased more significantly in the treatment group than in the control group. These results suggested that Lactiplantibacillus plantarum CBT LP3 and Bifidobacterium breve CBT BR3 may be effective in alleviating obesity in dogs.

Effect of genistein supplementation on microenvironment regulation of breast tumors in obese mice

Obesity is an important risk factor for breast cancer in women before and after menopause. Adipocytes, key mediators in the tumor microenvironment, play a pivotal role in the relationship between obesity with cancer. However, the potential of dietary components in modulating this relationship remains underexplored. Genistein, a soy-derived isoflavone, has shown promise in reducing breast cancer risk, attenuating obesity-associated inflammation, and improving insulin resistance. However, there are no reports examining whether genistein has the ability to reduce the effects of obesity on breast tumor development. In this study, we constructed a mammary tumor model in ovariectomized obese mice and examined the effects of genistein on body condition and tumor growth. Moreover, the effects of genistein on the tumor microenvironment were examined via experimental observation of peritumoral adipocytes and macrophages. In addition, we further investigated the effect of genistein on adipocyte and breast cancer cell crosstalk via coculture experiments. Our findings indicate that dietary genistein significantly alleviates obesity, systemic inflammation, and metabolic disorders induced by a high-fat diet in ovariectomized mice. Notably, it also inhibits tumor growth in vivo. The impact of genistein extends to the tumor microenvironment, where it reduces the production of cancer-associated adipocytes (CAAs) and the recruitment of M2d-subtype macrophages. In vitro, genistein mitigates the transition of adipocytes into CAAs and inhibits the expression of inflammatory factors by activating PPAR-γ pathway and degrading nuclear NF-κB. Furthermore, it impedes the acquisition of invasive properties and epithelial‒mesenchymal transition in breast cancer cells under CAA-induced inflammation, disrupting the Wnt3a/β-catenin pathway. Intriguingly, the PPAR-γ inhibitor T0070907 counteracted the effects of genistein in the coculture system, underscoring the specificity of its action. Our study revealed that genistein can mitigate the adverse effects of obesity on breast cancer by modulating the tumor microenvironment. These findings provide new insights into how genistein intake and a soy-based diet can reduce breast cancer risk.

Nesfatin-1 expression and blood plasma concentration in female dogs suffering from cystic endometrial hyperplasia and pyometra and its possible interaction with phoenixin-14

BackgroundNesfatin-1 is a neuropeptide that regulates the hypothalamic-pituitary-gonadal axis and may play a role in uterus function. It is co-expressed with other peptides, such as phoenixin, which can influence sex hormone secretion. Our previous research has confirmed that phoenixin-14 is involved in the development of cystic endometrial hyperplasia (CEH) and pyometra in dogs. Therefore, based on the similarities and interactions between these neuropeptides, we hypothesized that nesfatin-1 might also regulate the reproductive system in dogs. This study aimed to determine the expression of nesfatin-1 and its interaction with phoenixin-14 in dogs with CEH or pyometra compared to healthy females, and concerning animals’ body condition score (BCS 4–5/9 vs. BCS > 5/9).ResultsThe analysis of nesfatin-1 in the uterus of bitches consisted of qPCR, western blot and immunofluorescence assays, and ELISAs. The results showed significantly higher nesfatin-1 encoding gene, nucleobindin-2 mRNA (Nucb2) and nesfatin-1 protein expression in overweight females and those suffering from CEH or pyometra compared to healthy animals. The immunoreactivity of nesfatin-1 was elevated in the uteri of bitches with higher BCS > 5/9. Moreover, nesfatin-1 blood concentrations increased in all examined overweight bitches. In the case of phoenixin signals, we found opposite results, regardless of the female body condition score.ConclusionThe etiology of CEH and pyometra are not fully known, although we have expanded the level of knowledge with respect to the possible interaction of nesfatin-1 and phoenixin in female dogs’ uteri. They interact oppositely. With increasing female body weight, the expression of nesfatin-1 in the uterus and its peripheral blood concentration increased. However, for female dogs affected by CEH and pyometra, a decreased level of phoenixin-14, irrespective of their body condition score is characteristic. This knowledge could be crucial in the development of biomarkers for these conditions, which may lead to earlier recognition.

Temperature and water availability induce chronic stress responses in Zebra finch (Taeniopygia guttata).

Animals initiate physiological mechanisms to re-establish homeostasis following environmental stress. To understand how bird physiology responds to abiotic stress, we quantified changes in hematological markers of chronic stress response and body condition of male zebra finches (Taeniopygia guttata) acclimated for 18 weeks to hot and cool temperatures (daytime temperature: 40°C and 23°C) with water available ad libitum or restricted during half of the active phase. Ambient temperature induced greater chronic stress than restricted water availability. While cool compared to hot temperatures induced higher numbers of heterophils and H : L ratios and declined total leucocyte counts, water restriction decreased the number of lymphocytes compared to water ad libitum. Body condition correlated with hematological parameters showing that birds with better condition had greater capacity to face environmental stress. Therefore, prolonged exposure to cool periods may result in chronic stress in zebra finches, especially, if body condition is weakened.

Effects of Breeding Habitat on the Abundance and Body Condition of Two &lt;i&gt;Pelophylax&lt;/i&gt; Species in Agriculture and Urban Landscapes

Amphibians play a key role in structuring biological assemblages of agricultural landscapes, but they are threatened globally by agricultural intensification. Numerous studies have considered the morphology of animals to be an indicator of the health of a population, but data about the differences in amphibian morphology in different breeding habitats in agricultural landscapes are still missing. Here, we investigated the differences in the abundance and morphology of Pelophylax plancyi and Pelophylax nigromaculatus in the three waterbody types: fields, forests, and parks in agricultural landscapes in Nanjing, China. Our results emphasize that both frog species in forests and parks had significantly higher body condition indexes than those in fields, whereas their abundance in fields habitat was higher than those of the two other habitat types. Our results demonstrated the importance of non-agricultural habitats (such as forests and parks) in agricultural landscapes to the persistence of both Pelophylax species. We recommend preserving field ecosystems by constructing diverse waterbodies other than fields in rural areas and maintaining microhabitats in parks and forests to improve amphibian biodiversity in Nanjing.

Body size and condition, not allochrony, affect temporal reproductive patterns in a prolonged breeding anuran

Body size and condition, not allochrony, affect temporal reproductive patterns in a prolonged breeding anuran