Body Of Experimental Animals Research Articles

Quality of cattle meat when feeding the mineral supplement “Stimulus +”

To find out the influence of the mineral supplement “Stimulus +” on the intensity of physiological processes and productivity of bulls. To achieve this goal, we set ourselves the following tasks: 1. To study the effect of the additive “Stimulus +” on the hematological parameters of experimental bulls. 2. Investigate the effect of the drug “Stimulus +” on the quantitative and qualitative indicators of meat (slaughter quality, morphological and chemical composition of carcasses) and its biological value. To study the effect of the mineral supplement “Stimulus +” on the productivity of animals. The obtained results make it possible to correct the diets of experimental animals, which will positively affect erythropoiesis, respiratory function, certain areas of protein, energy, and hydrocarbon metabolism in young cattle, which will increase their productivity and improve the quality of beef obtained from them. Changes in the biochemical parameters of the blood of animals fed the mineral supplement were revealed. It is established to increase the productivity of animals by feeding them a mineral supplement, which indicates a more rational use of physiological resources of the body of experimental animals. It was found that feeding the animals led to an increase in slaughter yield by 3.8 % compared to control. It was found that during long-term storage (14 days) in the meat of animals of the control group, several changes indicate the beginning of spoilage of meat, and the meat of animals fed with mineral supplements was of good quality, as well as veterinary assessment was the best and more resistant to spoilage during storage. In order to prevent microelementosis of fattening cattle for fattening, increase their productivity, improve the physicochemical and veterinary-sanitary indicators of meat, and the profitability of production, we recommend adjusting their diets, according to previous studies.

EVALUATION OF PATHOGENICITY OF NODULE BACTERIA STRAIN RHIZOBIUM LEGUMINOSARUM G 222

Objective. Investigate pathogenic (non-pathogenic) properties of a new strain of nodule bacteria Rhizobium leguminosarum G 222. Methods. Microbiological (obtaining a suspension of R. leI guminosarum G 222, determination of its titre by opacity optical standard and by seeding on a digest medium; microscopy of stained imprint smears of internal organs of experimental animals and seeding of tissue samples on a digest medium), pathoanatomical (to determine possible invasiveness and dissemination of bacteria in the tissues of internal organs of animals — in the model of outbred white mice after administration of a suspension of live bacterial cells (oral doses from 0.5 × 109 to 5 × 109 CFU in 0.5 cm3 of sterile isotonic sodium chloride solution per mouse and intraperitoneal doses from 1 × 109 to 5 × 109 CFU/0.5 cm3) and statistical. Results. Over 15 days of observations after administration of a suspension of live bacterial cells, no death of experimental animals was registered. The strain did not lead to any changes in the general condition of the mice. No changes in their behaviour were reported. Fifteen days after the start of the study, it was found that this strain is non-infective (non-invasive), does not disseminate and does not reproduce in the body of experimental animals. Oral and intraperitoneal doses of a suspension of live bacterial cells did not result in bacterial invasion of animal internal organs. No retrocultures were registered. Gross examination did not find characteristic changes in the internal organs of the experimental animals. The obtained results indicate the avirulence of the strain for the studied warm-blooded animals (intraperitoneal LD50 > 5 billion cells/mouse, oral LD50 > 5 billion cells/mouse). Conclusion: According to the results regarding the lack of virulence and according to regulations, the new strain R. leguminosarum G 222 belongs to the group of avirulent microorganisms that are not able to invade the internal organs of studied warm-blooded laboratory animals and can be considered nonpathogenic and used as a basis for microbial preparations to increase crop yields.

Impact of lead acetate and sodium and potassium stearates on lipid peroxidation processes in the body of experimental animals

Introduction. Given the significant prevalence of lead in the environment, research in this area has significant social and economic importance. Lead compounds are characterized by high toxicity and increased ability to cumulate in ecosystems, humans, and animals. Lead enters the human body with food, drinking water, atmospheric air, and smoking. Lead causes pathological changes in the nervous system, blood-forming organs, kidneys, etc. Materials and methods. The experiments were carried out on four groups of white female rats, each included seven animals, weighing 150-200 g. The first group of animals was a control. The second group consumed dechlorinated water from the city water supply, followed by lead acetate. The animals from the third and fourth groups drank the same water with sodium stearate and potassium stearate content in a dose of 1/250 LD50. After the 40th-day of the use of these waters, the animals were orally administered lead acetate at a dose of 7 mg/kg. The levels of lipid peroxidation biomarkers were studied by studying the content of diene conjugates (DC) and malondialdehyde (MDA) in blood serum, liver, and kidney homogenates. Results. The administration of 1/2 acetate LD50 to lead in experimental rats drinking water with stearates was accompanied by a significant increase in the DCs concentration and (MDA) in animals. Higher concentrations of LPO products were observed in the group of animals that consumed water from potassium stearate. Conclusions. 1. With the oral administration of lead acetate against the background of drinking water containing stearates at a dose of 1/250 LD50, an increase in lipid peroxidation indices was noted compared with the control group. 2. Higher concentrations of LPO products were observed in the group of animals consuming water from potassium stearate.

Bioregulatory approach to stimulating sport horses

The presented work presents the results of the effectiveness of the bioregulatory approach in stimulating sports horses. The aim of the study was to find a way to safely restore the energy balance and prevent the occurrence of inflammatory processes in the musculoskeletal system during significant physical exertion. Sport horses spend a lot of energy not only in competition, but also during daily training. Such loads require a high supply of energy. Often the body uses its last reserves to compensate for its energy needs, which leads to exhaustion and the appearance of various diseases. First of all, diseases occur in the musculoskeletal system, because it is the limbs that bear the greatest load during training. As you know, various synthetic doping is introduced to keep animals in a sporty tone. Their action is not long-lasting, but it has many side effects for the animal's body and leads to disruption of the functioning of organs and systems. Today, there is an alternative to such stimulation methods thanks to a bioregulatory approach in the treatment of animals using preparations containing exclusively natural ingredients. To confirm the effectiveness of the use of bioregulatory drugs, clinical and laboratory studies of animal blood (general, biochemical, cytochemical) were carried out. The main changes in laboratory parameters were obtained by cytochemical analysis of such enzymes as GPDH, SDH. These enzymes are among the main indicators of the intensity of energy metabolism in the horse's body. The quantitative composition of basophils and eosinophils has also changed, which indicates a decrease in the level of the inflammatory process in the body of experimental animals. The results obtained prove the effectiveness of the investigated drugs and their direct influence on the energy balance of the “horse-athlete”. Thanks to the natural composition of the components of the preparations used, side effects on the body can be avoided and used without restrictions throughout the horse's sports career.

ACUTE TOXICITY OF A NEW BENZIMIDAZOLE DERIVATIVE WITH ANTITHROMBOGENIC PROPERTIES

The paper presents the results of studying the toxicological profile of a new pharmaceutical substance RU891 with antithrombogenic properties. The minimum toxic dose (TDmin) for RU-891 by peroral administration is 230 mg/kg < TDmin ≤ 460 mg/kg. In experiments on the acute toxicity of the compound RU-891, the nature and severity of its damaging effect on the body of experimental animals were established and the safety of its action was evaluated. When studying the toxic effect of the test sample, the tolerated and toxic doses of RU-891 were determined, and the most sensitive organs and systems of the body were identified.

Evaluation of the activity of antioxidant enzymes during chronic oral administration of a technical product derived from triazoles in the rats

Introduction. Entering the body in various ways, pesticides, being biologically highly active compounds, can pose a real danger to public health, causing changes in non-specific biochemical reactions of metabolism occurring in all living cells. The antioxidant system, represented as a balance of lipid peroxidation and antioxidant protection (POL - AOZ), is one of the metabolic regulatory mechanisms of these responses. The aim of the study was to study the effect of a technical product (TP), a derivative of triazoles, on the acti-vity of antioxidant enzymes in the rat body, under its repeated oral intake in a chronic 12-month experiment. Material and methods. A chronic (12 months) experiment was performed on male rats with a bodyweight of 200-210 g at the beginning of the study. Tested doses: 5.0, 16.0 and 55.0 mg/kg of body weight (1 control and 3 experimental groups, 20 individuals each). In the dynamics of the experiment, after 1, 3, 6 and 12 months, the state and behavior of animals, water and food consumption were observed. Changes in the enzymatic indices of the body’s antioxidant defense system (superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase) were registered. Results. It was found that TP at a dose of 5.0 mg/kg of body weight does not cause significant changes in the activity of antioxidant enzymes, doses of 16.0 and 55.0 mg/kg of body weight cause an increase in the activity of superoxide dismutase (SOD) and a decrease in the activity of catalase in the body of experimental animals compared to control animals. Discussion. In the conducted chronic experiment, it was found that the studied TP at a dose of 5.0 mg/kg of body weight does not cause significant changes in the activity of the studied antioxidant enzymes in the body of rats. The introduction of TP at doses of 16.0 and 55.0 mg/kg of body weight causes a significant change in the activity of such antioxidant enzymes as SOD and catalase. Our results are consistent with the literature data, according to which the cell quickly reacts to oxidative stress by increasing the activity of SOD, and SOD is considered even as a stress protein synthesized in response to oxidative stress. Conclusions. The conducted research shows the feasibility of studying antioxidant enzymes’ activity in the mammalian body in sanitary and toxicological studies to increase the reliability of the developed hygienic standards of xenobiotics in environmental objects and food products.

Вплив речовин гумінової природи на вуглеводний обмін при алоксан-індукованому цукровому діабеті у щурів

The article presents study results of the effect of humic nature biologically active additive «Humilid» on carbohydrate metabolism, namely on glucose, insulin and C-peptide in alloxan-induced diabetes mellitus in rats. The purpose of the experiment was to investigate how “Humilid” affects carbohydrate metabolism in rats; to study and analyze the effect of the humic nature biologically active substance «Humilid» on metabolic processes. In the work we used biochemical methods for studying the blood of experimental rats. Adult male white rats were taken for the experiment, from which four groups were formed. The experiment lasted three weeks, during which the animals from the first experimental group received daily in addition to the main feed an aqueous solution of feed additive «Humilid» at dose 5 mg / kg of body weight of the active substance, and had free access to water. Rats of the second and third groups were injected with alloxan as a single intraperitoneal injection. The rats of the third group additionally received an aqueous solution of feed additive «Humilid». It was identified that “Humilid” has an antioxidant activity, which may be one of the mechanisms of its antidiabetic action, as it is known that alloxan causes free radical damage to β-cells with weak antioxidant protection. The use of a biologically active feed additive of humic nature was found to have a hypoglycemic effect in both healthy animals as well as in rats with alloxan-induced diabetes mellitus. It was found that the use of “Humilid” in rats enhances energy processes in their bodies of experimental animals. There was a decrease in glucose content in rats from second group by 11.9% (P ≤ 0.001) compared with the control one. At the same time, the glucose content in animals from third and fourth groups was higher by 35.9% (P ≤ 0.001) and 20% (P ≤ 0.001) higher than in animals from the control one. The insulin concentration in the blood of rats from the second group was 7.4% higher (P ≤ 0.05) compared to the animals from first group. The concentration of C-peptide was within the reference values. The levels of insulin and C-peptide concentration in animals of the third and groups were lower by 47.8% (P ≤ 0.001) and 32.4% (P ≤ 0.001) and 55.5% (P ≤ 0.001) and 37.5% (P ≤ 0.001) in comparison with animals of first group. A pronounced hypoglycemic effect, which was due to an increase in the sensitivity of tissues to glucoregulatory hormones and an increase in the body’s tolerance to excessive intake of carbohydrates. The obtained results of the study indicate the regulatory effect of humic feed additive «Humilid» on key links in the regulation of carbohydrate metabolism, which is characterized by the decrease in blood glucose level due to activation of the synthesis and secretion of insulin and C-peptide.

ИЗУЧЕНИЕ ТОКСИКОЛОГИЧЕСКИХ СВОЙСТВ ДЕЗИНФИЦИРУЮЩЕГО ПРЕПАРАТА АСТРАДЕЗ БИОКСИ В ЛАБОРАТОРНЫХ ОПЫТАХ

The article presents the results of toxicological studies, investigated the cumulative properties of disinfectant Astradez Bioxy, as well as its effect on the body of experimental animals: white male rats and white mice. It has been established that disinfectant Astradez Bioxy is a moderately toxic compound (3rd hazard class), does not have a negative effect on the body of animals and can be used for disinfection in veterinary practice. According to the degree on human impact Astradez Bioxy belongs to the 4th class of low-hazard substances.

Intensity of ecotoxicants’ accumulation in internal organs and milk of dairy cows in the North-West of Transurals

The work of the agrarian sector of the economy is impossible without deep awareness of the serious environmental situation threat of anthropogenic factors’ influence due to the increasing intensification system of processes in animal husbandry. In addition to understanding the processes occurring in connection with the increase in anthropogenic load, real-time information about the real situation in the agroecosystem is necessary. Forecast of decision-making, consequences of certain actions. Control and monitoring of changes in the environmental situation. Therefore, the current control and the real system for assessing the ecological situation is important for the correct ecological activity of the agrarian organization. The greatest ecotoxicants’ concentration is observed in the body of experimental animals, most of all in the respiratory and excretory organs. The minimum level of ecotoxicants was observed in muscle tissue, hence it is an environmentally friendly livestock product. The concentration of arsenic, mercury in the experiment is at the level of trace indicators. The level of ecotoxicants’ accumulation in drinking cow’s milk was established, the correlation between the pollutants’ level in the product and the location of agricultural production, season and production time was found.

Стан процесів метаболізму у лабораторних щурів за застосування амарантової олії та Гуміліду

The search for new natural non-toxic biologically active substances for animals used to correct and stimulate metabolic processes, and through it the ability to control the growth and animals’ productivity, the state of their natural resistance is the main task of the present. The paper presents study results on the effect of cold-pressed amaranth oil and biologically active additive of humic nature «Humilid» when used simultaneously on the general body condition, growth indicators of laboratory rats, morphofunctional and biochemical parameters of their blood. To achieve this goal, the following tasks were set: – to investigate how cold-pressed amaranth oil affects the metabolic processes in the body of laboratory rats; – to conduct research and analyze the effect of the cold-pressed amaranth oil and a humic nature biologically active substance «Humilid» simultaneous use on the indicators of their homeostasis. To accomplish the tasks, the following research methods were used: physiological, morpho-functional, and blood biochemical analysis in experimental laboratory rats. Sexually mature male white rats of 2 months age were used in the experiment. For the study, three groups of animals were formed. The experiment lasted four weeks, during which the animals of the first experimental group received daily, in addition to the main feed settled amaranth oil in the amount of 0.1 ml per rat, and free access to the drinking water. Rats of the second experimental group in addition to the main feed received settled amaranth oil in the amount of 0.1 ml, and free access to the drinking of 0.005% solution Humilid. It was found that the simultaneous use of cold-pressed amaranth oil and Humilid in laboratory rats had a positive effect on the state of erythron, erythrocytes count increased by an average of 28% (P < 0.001), hemoglobin concentration by 10% (P < 0.05). At the same time in animals the biosynthetic function of the liver improved, the total protein content in the blood significantly increased by 10% (P < 0.05), and the amount of albumin by 25% (P < 0.01). Energy processes in the body of experimental animals were increased, that pointed a decrease in blood glucose levels by 22% (P < 0.05). The obtained data obtained indicates that the simultaneous use of amaranth oil and Humilid had a positive effect on the state of homeostasis and metabolic processes in the body of laboratory rats. Thus for them synergistic action is noted.

ДО ПИТАНЬ ВПЛИВУ ПОЄДНАНОЇ ДІЇ ЕЛЕКТРОМАГНІТНОГО ВИПРОМІНЮВАННЯ ТА ШУМУ НА СТАН МОРФОЛОГІЧНИХ ЕЛЕМЕНТІВ КРОВІ

The aim. Study of hematological parameters under conditions of combined action on the body of experimental animals. Subject and methods. The effect of electromagnetic radiation on the state of morphological elements of the blood. Hematological, statistical methods. Results. A two-month experiment was performed on white rats exposed to EMR at the levels of 100, 500 and 2500 μW/cm2 and acoustic noise of 85 dBA. The structure of the leukogram changed due to changes in the absolute number of leukocytes (increase), lymphocytes (decrease), monocytes (decrease) and granulocytes (decrease). Since lymphocytes belong to immunocompetent cells and assessing the nature of their changes during 60 days of the experiment, we can assume that there is an increase in regenerative processes in the lymphocyte population and activation of the immune system by damaging factor, followed by gradual depletion of compensatory mechanisms and suppression of suppressor mechanisms. Decreased hemoglobin levels and a decrease in the average concentration of hemoglobin in erythrocytes may indicate low blood O2 saturation and the development of anemia, with the subsequent development of dysfunction of all organs and systems of the body. Conclusions. The nature of changes in hematological parameters during the experiment may be a manifestation of mobilization of functional systems and the formation of adaptive responses aimed at maintaining the stability of homeostasis in the studied factors with possible prolongation of their further disruption, suppression of regenerative processes in the blood and depletion of compensators, or the development of various reparation reactions that provide protective - restorative functional systems to maintain the dynamic balance of the organism with the environment.

Morphogenesis of the internal organs of mice with the use of adaptogens and physical activity

Maximum physical exercise has a negative effect on physiological and morphological processes in the body. However, in some cases, the body responds with adaptive properties, which leads to smoothing out the negative effects of exercise. Establishing causes and factors that positively affect the processes of adaptation to increased activity and identifying the mechanisms of this process is one of the urgent problems of adaptation. Adaptation processes have a certain structure, and therefore, the goal of our research was to study the physiological processes and morphogenesis of individual organs and systems of the animal organism. One of the first to conduct a comprehensive assessment of the histological changes in experimental animals when giving adaptogens against the background of maximum activity. As adaptogens, the components of the military nature were used, tincture of safflower-like leuzea and ovesol, which were administered for 28 days at a dose of 2 to 6 μl according to the developed scheme. In this case, ovesol was used only at the final stage of the experiment from 22 to 26 days. It was found that ultra-high physical exercise leads to histological and physiological changes in the body of experimental animals.

ОБОСНОВАНИЕ ПРИМЕНЕНИЯ МИКРОЭЛЕМЕНТСОДЕРЖАЩЕЙ ДОБАВКИ ДЛЯ ПОВЫШЕНИЯ ПРОДУКТИВНОСТИ ЖИВОТНЫХ

A test was carried out of an additive containing trace elements for feeding cows with feed in order to increase the productivity and quality characteristics of the milk obtained from them. Stud-ies have established that the tested feed additive does not have a negative effect on the body of ex-perimental animals and can be recommended for use in dairy cattle breeding. When using an addi-tive containing microelements, the mass fraction of fat, protein, dry fat-free residue in cow's milk increases.

The Characterization of tissue responses to titanium meshes with different through porosity in the experimental study

During the experiment, three artificial defects were created on the outer surface of the jaw body of experimental animals. Two wells were filled with bone substitute materials, one well was not filled with anything and remained as a control. The defects formed and filled with materials were closed with titanium membranes with different through porosity. in order to study the effect of titanium membranes with varying degrees of porosity on osteoreparative processes in the area of defects, histological studies were carried out. As a result, it was determined that bone recovery was the same both in the group where titanium membranes with a pore diameter of 0.25 mm were used and in the group where titanium membranes with a pore diameter of 1.0 mm were used.

Comparative characteristics of the use of bone-substituting materials based on mineral and collagen

The purpose of this study was to carry out a comparative assessment of two types of bone substitute materials for targeted bone regeneration, on a mineral basis and on the basis of collagen. During the experiment, artificial defects were created on the outer surface of the jaw body of experimental animals. Two defects were replaced with osteoplastic materials with different degrees of mineralization, one defect remained under the clot. Histological examinations of biopsy specimens were performed. It was revealed that when using demineralized bone substitute materials, the processes of bone tissue repair occur more intensively.

THE ACID-BASE PROCESSES' CHANGES IN THE BODY OF WHITE RATS UNDER THE INFLUENCE OF NITROGEN-CONTAINING SURFACE-ACTIVE MATERIAL

Relevance. Everyday contact of the population with surfactants (SAS) in drinking water poses the problem of timely and prompt substantiation of pre-nosological highly sensitive indicators of early manifestations of biological activity of detergents and operational control over the health of the population and the environment. But today the mechanisms of biotransformation, toxicodynamics, toxicokinetics, and metabolic processes that underlie the formation of structural and metabolic disorders when exposed to a surfactant, taking into account possible long-term effects, have not yet been fully elucidated.
 Objective: to investigate the effect of nitrogen-containing surfactants on redox processes in the body of experimental animals.
 Materials and methods. The experiments were carried out on 620, and acute experiments on 128 white rats (weight 180-220 g). We used four ionic nitrogen-containing surfactants with specified technical and physicochemical characteristics: FOM 9, FOM 9-4, FOM 9-12, and FOM 9-20. Doses were chosen so as to determine the lethal effect in the lethal dose (LD) range from 0 to 100. The LD50 was calculated. The substances were introduced into the stomach in pure form using a metal probe. The animals were observed for up to 15 days. The time of death of the animals and the total amount of the introduced substance was recorded. The animals were subjected to postmortem examination. Redox processes were qualitatively assessed by the activity of enzymes: cholinesterase, cerulose plasmin, lactate dehydrogenase, malate dehydrogenase, succinate dehydrogenase, peroxidase, catalase, cytochrome oxidase, by the content of SH-groups in the blood, by the concentration of biogenic monoamines.
 Results. Nitrogen-containing surfactants caused a change in peroxidase activity both upwards and downwards. In all cases, 1/1000 LD50 was inactive. On the 15th day of the experiment, neonol FOM 9-12 reduced the activity of the enzyme, and other substances did not affect it. By the end of the subacute experiment, neonol FOM 9-4 and neonol FOM 9-12 were reduced, and neonol FOM 9-20 increased peroxidase activity. A similar effect was on the activity of catalase: in all groups, except 1/1000 LD50, on day 30 there was a decrease in its activity. Cholinesterase activity increased. For the content of SH-groups in the blood on the 15th day, there was a tendency to decrease, which turned into significant differences on the 30th day in 1/10 LD50. The effect of 1/100 and 1/1000 LD50 did not violate the content of SH-blood groups. A similar effect was on the content of glutathione in the blood. In a subacute experiment, in groups, 1/10 and 1/100 LD50, the content of norepinephrine, tryptophan, serotonin in the liver increased and DOPA and dopamine decreased. The dynamics of adrenaline did not change. The content of dopamine and norepinephrine increased to a lesser extent in the brain; DOPA and adrenaline did not differ from the control; tryptophan increased only under the influence of FOM-9. 1/1000 LD50 did not affect the dynamics of the content of biogenic monoamines. The tested drugs have a similar effect on the body.
 Conclusions. A more toxic substance in a subacute experiment is FOM-9. The severity of violations in the dynamics of monitoring the activity of enzymes has a close dose-dependence. The effective dose is set at 1/10, the threshold – 1/100, and the inactive – 1/1000 LD50. Common features of the biological action of nitrogen-containing surfactants are the violation of redox processes, bioenergy, oxidative phosphorylation, which under appropriate conditions lead to the pathology of vital organs, functions, and systems of the body.

The biological action of solid solutions (on the example of cadmium-mercury-tellurium alloy)

Solid solutions are widely used in various industries. Experimental studies have been conducted to study the biological effects of warm-blooded animals (rat, mouse) solid solution based on cadmium (7%) mercury (52%) and tellurium (41%) (CMT). The purpose of the study: To investigate the biological effect of the solid solution (CMT) on the body of experimental animals. Material and methods. On the models of acute and subchronic exposure, the biological effect of CMT on the organism of warm-blooded animals (rats, mice) was evaluated. Studies have been carried out to establish toxicometry parameters (acute toxicity, the threshold for acute inhalation and intragastric effects, cumulative properties). In subchronic experiments, local and general toxic effects were investigated. The work used biochemical, physiological, toxicological, pathomorphological, statistical research methods. Results. According to acute experiments, the investigated substance belongs to the 4th hazard class (low-hazard compound). The threshold of acute inhalation action for rats is 46.20 mg/m3, and the intragastric effect for rats is 5000 mg/kg. CMT has moderate cumulation (Kcum = 4.79), does not irritate the skin. The compound was found to have a general toxic effect, which is based on functional disorders of the nervous system, liver, and kidneys. Discussion. The results of the study indicate that solid CMT solution has a polytropic effect on the warm-blooded organism. Conclusion. With prolonged exposure, the CMT aerosol can harm the body of workers, it is necessary to provide general preventive measures that prevent the aerosol of solid solutions from entering the body: automation, mechanization, sealing of production, adequate ventilation of production rooms, the use of personal protective equipment, the passage of mandatory periodic medical examinations by workers, and monitoring the concentration of CMT in the air of the working area should be carried out according to the MPC - 1.0 mg / m3 (mercury vapor).

The toxicity of the technical product, derived triazolinones

Introduction. Technical products that are part of pesticides recommended for use in agriculture must undergo a comprehensive sanitary and Toxicological examination, which is the basis for preventing the adverse effects of pesticides on the health of workers and the population, as well as on the sanitary state of the environment. Purpose of research - the study of the biological effect of the technical product derived triazolinthionov, with its repeated oral intake in mammals (rats), justification of the permissible daily dose (DSD) for humans. Material and methods. Chronic (12 months) experiment was conducted on male rats with a body weight of 200-210 g tested doses: 5.0; 50.0 and 500.0 mg/kg body weight (1 control and 3 experimental groups and 20 individuals each). In the dynamics of the experiment, we observed the condition and behavior of animals, water and food consumption, fixed the timing of death, recorded changes in body weight, physiological, biochemical and hematological parameters. Results. It was found that the dose of 5.0 mg/kg body weight does not cause significant changes in all studied parameters, doses of 50.0 and 500.0 mg/kg body weight had a polytropic effect on the body of experimental animals. Discussion. The studied technical product at repeated intake in doses of 50,0 and 500,0 mg/kg of body weight causes changes in the state of the Central nervous system of animals (statistically significant changes in SPP, total activity, path length, rest time), as well as changes in carbohydrate, lipid, and lipoprotein metabolism in the body, as evidenced by statistically significant changes in biochemical and hematological indicators. Consequently, doses of 50,0 and 500,0 mg/kg of body weight have a polytropic effect on the body of male rats and are effective. The dose of 5.0 mg/kg of body weight, when administered in animals of the experimental group in comparison with animals of the control group, there are no changes in all the studied parameters throughout the experiment, is accepted as invalid. On the basis of an inactive dose of 5.0 mg/kg of body weight and a reserve factor of 100, we have scientifically justified DSD for humans at the level of 0.05 mg/kg. Conclusion. Studies have shown that long-term repeated oral administration of the studied product into the body of animals (male rats) at a dose of 5.0 mg per 1 kg of body weight does not cause statistically significant changes in all the studied parameters, so the indicated dose is invalid. Doses of 50,0 and 500,0 mg/kg MT have a polytropic effect on the body of male rats and are effective. DSD for humans at the level of 0.05 mg/kg is justified based on the inactive dose at the level of 5.0 mg per 1 kg of body weight, established in a 12-month chronic experiment conducted on male rats, and the reserve coefficient of 100 (taking into account the unexpressed specific and long-term effects).

Effect of Platinum Nanoparticles on the Structure of Protein Molecules and Regulation of the Tone of Brain Vessels in Experimental Animals.

The effects of platinum nanoparticles on the morphological structures of the solid phase of blood serum and the tone of cerebral microvessels as indicators of the dynamics of homeostasis were studied on outbred albino male rats (n=40) weighing 300-350 g. Platinum nanoparticles were injected to experimental animals in 1 ml of physiological saline. For systemic BP measurements and blood sampling, the femoral artery was isolated and catheterized. The study of solid phase structures of blood serum was conducted by the method of cuneiform dehydration. The results showed that injection of platinum nanoparticles significantly affected the body of experimental animals.

Histological examination and evaluation of biocompatibility of 3-D printed implants in the experiment.

Background. High levels of musculoskeletal injuries, pathologies and various diseases of bone tissue encourage researchers around the world to actively seek out new and improve existing implant materials for high-quality reconstructive and restorative operations on bone tissue. Objective. Histological examination and evaluation of biocompatibility of 3-D printed implants after implantation in experimental animals. Methods. 3-D printed materials were implanted into the white laboratory rats Wistar for 1, 4, and 12 weeks. The cellular reactions of the organism and possible changes in the structure of the test specimens after implantation were studied by light microscopy by histological micropreparation analysis. Results. Histological examination was performed and the nature and dynamics of cellular responses after implantation of 3-D printed materials were evaluated. Conclusion. It was found that cellular migration and germination of connective tissue tendons deep into the implants due to the porous structure of 3-D printed material based on ceramics, resulted in partial degradation, which slightly increased the intensity of cellular reactions at all study periods. It was found that the intensity of cellular reactions was minimal in the early stages of the study around polymer-based 3-D printed material, but 12 weeks after implantation, cellular responses were increased. It is shown that implantation of 3-D printed materials into the body of experimental animals led to the development of cellular responses typical of aseptic inflammation, which testified to their biocompatibility.