Oxygen for the aerobic treatment of wastewater in High Rate Algal Pond (HRAP) systems arises from biogenic production by microalgae during photosynthesis and from absorption of atmospheric oxygen through the air/water interphase. The total quantity of available oxygen per m 2 and day determines the permissible BOD loading rate of a HRAP. The quantity of biogenic oxygen production, has for the first time been measured in a closed bioreactor and open chemostatic cultures. Good agreement was obtained between the two methods where more than 1.92 g O 2 g(dry wt) was produced by the microalgae. This was significantly higher than oxygen yields calculated from a generalized compositional formula of algal biomass. In combination with a predictive mathematical model, it was possible to calculate the potential biogenic oxygen production per season for any latitude. This may serve as the basis of feasibility studies on application of HRAP systems.
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