This article describes effect of the blue light on a human health. This effect is emmiting by electrical devices and has a negative impact on person, even if it does not fall into your eyes. This work consists on comparison study of blue radiation spectrum and impact on human organism. It helps to define permissible level of energy dependence on light radiation. The conception of biological impact connected with melanopsin, which one has rods and cones. For humans, nonvisual biological effects, which can be parameterized by the circadian action factor (CAF), are important for their health and work performance. When we spend a lot of time in front of screens of devices, we are receiving radiation of blue light. The article analyses questions associated with biological exposure to radiation from some light emitting diode and lamp light sources with various radiation spectrum (correlated colour temperature Tcc). The biological exposure concept is connected with melanopsyn containing receptors on the eye retina, signals from which arrive to epiphysis and control concentration of the hormone melatonin in the blood. Circadian rhythms are seen at every level of biology, from single cells to complex behaviors. The timing of every biological function in mammals is governed by the master clock in the suprachiasmatic nuclei (SCN), which has an intrinsic period of slightly longer than 24 hours. The light/dark pattern incident on the retina synchronizes the SCN to the 24-hour local time, coordinating and enabling diverse biological functions to occur at the correct time of day and night for optimum species survival. A wide range of modern maladies, from sleep disorders to cancer, has been linked to light-induced circadian disruption. Light has, however, been defined only in terms of the human visual system, not the circadian system. Light source and systems development should consider the needs of both the visual and non-visual systems. Certain signals are fulfil function of controlling the concentration chromatophorotropic hormone within blood. As a basis for calculation were included photochemical and electrical processes in amphiblestrodes, physiological human parameters, as well as coefficient of circadian efficiency, coefficient of growth-inhibitory activity of melatonin and biological equivalent. The optical information is essential for physiological systems of humans, because it not only provides the visual information but also affects their physical, physiological, and psychological behaviors, in which researchers call the latter “non-visual biological effects of light”. In the evolution of lives on earth, the sun and its spectrum, and the alternation of day and night play an important role in the adaptation of human to the natural environment. As receptors of light, human eyes are deeply influenced by standard light, to which the structure and function of human eyes are developed more accustomed during the long-term daily work.