Blue Product Research Articles

“All-on-a-Tube” POCT of Salmonella in large-volume sample

Point-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive Salmonella detection in large-volume samples, where the entire bacterial detection progress from separation to detection, was performed within the tube. The bottom of this centrifuge tube was assembled with a flexible stirrer containing a pair of circular magnets, which was rapidly rotated using a DC motor to produce the vortex for simultaneous mixing and capture of target bacteria. Besides, immune manganese dioxide nanoflowers were synthesized and used to label target bacteria, followed by mimicking catalyze colorless TMB substrate into blue TMBox product. The product image was captured and analyzed by a smartphone App to quantitatively determine the target bacteria. This POCT centrifuge tube effectively achieved a separation efficiency of approximately 80 % for target bacteria from a 10 mL sample, enabling the detection of target bacteria within the range of 1.3 × 101 to 1.3 × 104 CFU/mL in 1 h, with a low detection limit of 13 CFU/mL. More importantly, this reconstructed centrifuge tube demonstrated its potential as a laboratory consumable for bacterial detection in routine screening owing to the features of low cost, easy operation and high integration.

Effect of Water Vapor Partial Pressure on the Structure of Blue Tungsten Reduction Products in Hydrogen

This study investigates the structural evolution of blue tungsten reduction products when exposed to hydrogen under controlled water vapor partial pressures. The reduction of WO₂.₉ was achieved through hydrogen atom diffusion at 1050 °C. The Results indicate that, at low water vapor partial pressure, the reduction process of blue tungsten initiates on the surface and progresses inward, creating surface cracks on the clusters. These cracks enable water vapor to interact with the reduced tungsten (W) at their base, leading to the formation of needle-like WO₂.₇₂ phases that grow outward. Under high water vapor partial pressure, the morphology of the acicular WO2.72 phase changes to a stacked structure. Additionally, W-OH bonds were identified in the reduction products, with the WO₂.₇₂ growth angles observed at approximately 12° relative to the tungsten particles, closely matching the interfacial model prediction of 13.41°. The results of this paper will provide theoretical support for the structural evolution of intermediate products during hydrogen reduction of blue tungsten.

Organic Two-Photon-Absorbing Photosensitizers Can Overcome Competing Light Absorption in Organic Photocatalysis.

Conventional organic photocatalysis typically relies on ultraviolet and short-wavelength visible photons as the energy source. However, this approach often suffers from competing light absorption by reactants, products, intermediates, and co-catalysts, leading to reduced quantum efficiency and side reactions. To address this issue, we developed novel organic two-photon-absorbing (TPA) photosensitizers capable of functioning under deep red and near-infrared light irradiation. Three model reactions including cyclization, Sonogashira Csp2-Csp cross-coupling, and Csp2-N cross-coupling reactions were selected to compare the performance of the new photosensitizers under both blue (427 nm) and deep red (660 nm) light irradiation. The obtained results unambiguously prove that for reactions involving blue light-absorbing reactants, products, and/or co-catalysts, deep red light source resulted in better performance than blue light when utilizing our TPA photosensitizers. This work highlights the potential of our metal-free TPA photosensitizers as a sustainable and effective solution to mitigate the competing light absorption issue in photocatalysis, not only expanding the scope of organic photocatalysts but also reducing reliance on expensive Ru/Ir/Os-based photosensitizers.

A colorimetric platform using highly active Prussian blue composite nanocubes for the rapid determination of ascorbic acid and acid phosphatase.

Cobalt-doped Prussian blue composite nanocubes (Co-PB NCs) were synthesized, which can quickly convert O2 to O2•- and 1O2. Due to the presence of cobalt and iron transition metal redox electron pairs, Co-PB NCs with high oxidase mimetic activity can rapidly oxidize the substrate 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue products (ox-TMB) without the assistance of unstable H2O2. Using ascorbic acid-2-phosphate trisodium salt (AAP) as a substrate, it can be converted to reduced ascorbic acid (AA) under acid phosphatase (ACP) hydrolysis, resulting in suppression of TMB oxidation. Therefore, an enzyme cascade signal amplification strategy for rapid colorimetric detection of AA/ACP was developed based on the high-efficiency oxidase-like activity of Co-PB NCs combined with the hydrolysis effect of ACP. The color changes at low concentrations of AA and ACP could be observed by the naked eye, and the detection limits of AA and ACP were 1.67μM and 0.0266 U/L, respectively. The developed colorimetric method was applied to the determination of AA in beverages and ACP in human serum, and the RSDs were less than 3%, showing good reproducibility. This work provides a promising strategy for the use of metal-doped Prussian blue composite material for the construction of rapid colorimetric sensing platforms that avoid the use of unstable hydrogen peroxide.

Nanozyme-based colorimetric and smartphone imaging advanced sensing platforms for tetracycline detection and removal in food

The presence of antibiotic residues poses a significant threat to food assurance, triggering widespread concerns. Therefore, the prompt and accurate detection and removal of antibiotic residues are essential for ensuring food safety. In this study, an aptmer modified triple-metal nanozyme (apt-TMNzyme) sensor was developed, which achieved a portable, visual, intelligent, and fast determination for tetracycline (TET). The proposed apt-TMNzyme exhibited willow leaf-like morphology, high specific surface area and excellent TET adsorption and removal properties. The experiments showed that the apt-TMNzyme had outstanding peroxidase activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product in the presence of H2O2, which provided a visual response signal to TET. This sensor was capable of quantifying TET within a concentration range of 0.2 nM–70 μM, achieving a detection limit of 7.1 nM under optimal conditions. When tested on real food samples, our sensor produced results that closely paralleled those achieved through high-performance liquid chromatography. To improve accessibility and user-friendliness, we also designed a colorimetric testing paper integrated with a smartphone application for intuitive and intelligent detection of TET, which enables the quantitative determination of TET in the concentration range of 0.003–60 μM, the detection limit was 5.1 μM. This integrated portable sensor not only streamlines the testing process, saving time and costs, but also offers a promising solution for rapid and sensitive detection of antibiotic residues.

Insight into the chemistry of Folin’s test for uric acid

Folin developed an analytical technique for uric acid spectroscopic determination. It is based on the interaction of uric acid with sodium tungstate in phosphoric acid. This produces an intense blue colour after alkalinization, whose concentration is measured. However, the structure of the organic product obtained was not mentioned. The present communication gives the references related to each reaction step, as well as information about the reaction products obtained in uric acid oxidations carried-out in acidic or in alkaline mediums. The reaction route is as follows: addition of tungstic acid to the C-C double bond, acidolysis of the organo-metallic ester, producing tungstic subacid and an epoxide. Oxirane opening to vicinal diol after reaction with water. Two carbinolamines result, which are broken by protonation at the nitrogen; carbonyl groups are formed at C-4 and C-5 with concomitant elimination of a urea molecule. This way alloxan monohydrate is formed. The blue products obtained are described.

Mn3O4-FeS2/Fe2O3 composite as peroxidase-mimics for the degradation of Bisphenol A

Due to the benefits of artificial peroxidase-like enzymes, Flower-like Mn3O4-FeS2/Fe2O3 composites with multiple active sites prepared by a one-step solvothermal method.The synergistic cyclic reaction between Mn and Fe on the catalyst surface enhances the generation of active radicals, which together promote the efficient degradation of BPA. In the presence of hydrogen peroxide (H2O2), Mn3O4-FeS2/Fe2O3 could generate hydroxyl radicals (·OH) and superoxide radicals (·O2-) in buffer solution of pH=4.5 and catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidation products (ox TMB). The steady-state kinetic constants (Km) of TMB and H2O2 were obtained by Michaelis-Menten and Lineweaver-Burk models, which are 1.34 mM and 1.00 mM, respectively. This reveals the nice catalytic efficiency of Mn3O4-FeS2/Fe2O3 as enzyme mimics. Under the condition of 10 mg Mn3O4-FeS2/Fe2O3 catalyst, 100 μL H2O2 and pH = 4.5, the degradation efficiency of Bisphenol A (BPA) reaches 100 % when treating 40 mL of 50 mg L−1 BPA for 30 min at room temperature. Moreover, after 5 cycles of degradation, the BPA degradation efficiency of 85.8 % was still maintained. Therefore, the as-prepared Mn3O4-FeS2/Fe2O3 sample could potentially be applied in wastewater treatment.

A colorimetric sensor array consisted of CaxMn1-xWO4 with high oxidase-like activity to detect antioxidants

Antioxidant detection is highly significant in practical applications. This study establishes an optical sensor array capable of simultaneous and convenient detection of multiple antioxidants in real samples. CaxMn1-xWO4 (CMW) synthesized via hydrothermal method with abundant surface oxygen vacancies and high internal electron transfer efficiency exhibits strong oxidase-like activity, which can oxidize TMB to blue product oxTMB using oxygen as reaction substrate. The presence of antioxidants weakens TMB oxidation, leading to a lighter blue color in the system. Therefore, CMW can be employed for colorimetric detection of antioxidants. Then CMW samples with varying Ca to Mn ratios were prepared, followed by the establishment of an optical sensor array. The detection systems were photographed, and PCA analysis of the RGB parameters was performed, enabling simultaneous qualitative and quantitative detection of nine kinds of antioxidants.

Copper Single-Atom Nanozyme Mimicking Galactose Oxidase with Superior Catalytic Activity and Selectivity.

Due to the low stability and high cost of some natural enzymes, nanozymes have been developed as enzyme-imitating nanomaterials. Single-atom nanozymes are a class of nanozymes with metal centers that mimic the structure of metal-based natural enzymes. Herein, Cu-N-C single-atom nanozyme (SAN) is synthesized with excellent peroxidase- and enhanced oxidase-like activities to mimic the action of natural galactose oxidase. Cu-SAN demonstrates stereospecific activity akin to that of natural galactose oxidase by oxidizing D-galactose and primary alcohol but not L-Galactose or other carbohydrates. The SAN can catalyze the oxidation of galactose in the presence of oxygen, producing hydrogen peroxide as a sub-product. The produced hydrogen peroxide then oxidizes 3,3',5,5'-tetramethylbenzidine catalyzed by the SAN, yielding the typical blue product. The relationship between absorbance and galactose concentration is linear in the 1-60µm range with a detection limit as low as 0.23µm. This strategy can be utilized in the diagnosis of galactosemia disorder and detection of galactose in some dairy and other commercial products. DFT calculations clarify the high activity of the Cu sites in the POD-like reaction and explain the selectivity of the Cu-SAN oxidase-like reaction toward D-galactose.

A colorimetric sensor based on multiple elements doped carbon dot nanozyme for rapid detection of 1-naphthol in human urine samples

The residual carbaryl in crops can cause serious damage to the human kidney and nervous system after entering the human body, which may be metabolized to 1-naphthol (1-NAP) and excreted through urine. 1-NAP is often used as the biomarker for carbaryl exposure, so the intake or leakage of carbaryl can be monitored by detecting the concentration of 1-NAP. Herein, Co, N, P ternary co-doped carbon dots (CoNP-CDs) derived from vitamin B12 were synthesized by a facile hydrothermal method. CoNP-CDs exhibited oxidase-like activity and excellent peroxidase-like activity, which was attributed to the Fenton-like reaction of Co2+/Co3+ and the presence of pyrrole N and P elements, which together provided multiple active sites for chromogenic substrates. Due to the dual enzyme-like activity of CoNP-CDs, hydroxyl radicals (OH) and superoxide radicals (O2−) were generated during the catalytic process, which could rapidly oxidize colorless 3,3′,5,5′-tetramethyl benzidine (TMB) to blue oxidation products (oxTMB). The α-carbon in 1-NAP can be attacked by OH, and the catalytic oxidation process of TMB can be inhibited by the consumption of OH, so that the blue color of the solution became lighter. Based on this principle, a smartphone-assisted colorimetric sensing platform was constructed for the detection of 1-NAP, and which resulted in a linear range of 1.07–37.3 μM and a visual detection limit of 0.68 μM. Moreover, the colorimetric sensing system showed satisfactory recoveries in the detection of human urine samples. The colorimetric sensing system owned the advantages of fast response, strong selectivity and simple operation, and provided a potential strategy for the on-site detection of 1-NAP.

Membrane separation assisted colorimetric/fluorescent detection of β-galactosidase-positive bacteria in milk and milk powder based on the oxidase-like activity of CoOOH nanosheets

Species-specific enzymes provide a substantial boost to the precision and selectivity of identifying dairy products contaminated with foodborne pathogens, due to their specificity for target organisms. In this study, we developed cobalt oxyhydroxide nanosheets (CoOOH NSs) for a dual-mode biosensor capable of detecting β-galactosidase (β-Gal)-positive bacteria in milk and milk powder. The sensor exploits the oxidase-mimicking activity of CoOOH NSs, where β-Gal converts the substrate β-D-galactopyranoside to p-aminophenol, reducing CoOOH NSs to Co2+ and inhibiting the formation of the blue product from 3,3′,5,5′-tetramethylben-zidine. Sensitivity was enhanced through membrane filtration and β-Gal induction by isopropyl β-D-thiogalactoside. The assay achieved a detection limit of 5 cfu mL−1 and demonstrated recoveries (90.7 % to 103 %) and relative standard deviations <5.7 % in milk and milk powder samples. These findings underscore the potential of the sensor for detecting β-Gal-positive bacteria in dairy products.

Near-infrared light-enhanced colorimetric signal amplification strategy for tumor marker detection based on MoS2/CuO/Au nanocomposite.

Anovel signal amplification strategy was developedby combining near-infrared light with MoS2/CuO/Au nanocomposite for building acolorimetric immunoassay. First, MoS2/CuO/Au nanocomposite was synthesized by precipitation and photoreduction methods and characterized by scanning electron microscopy (SEM) and X-ray powder diffraction (XRD). MoS2/CuO/Au nanocomposite has oxidase-like activity and can oxidize TMB to form a blue product (TMBox). Further, the catalytic oxidation of TMB was accelerated under near-infrared (NIR) laser radiation. The sandwich-type colorimetric immunoassay was constructed using MoS2/CuO/Au nanocomposite. Under the enhancement of near-infrared light, carcinoembryonic antigen (CEA) was sensitively detected in the range 0.1 to 40ng/mL with the limit of detection of 0.03ng/mL. Moreover, the immunosensor has excellent selectivity and anti-interference, good repeatability, and stability.

Trimetallic FeCoNi Metal-Organic Framework with Enhanced Peroxidase-like Activity for the Construction of a Colorimetric Sensor for Rapid Detection of Thiophenol in Water Samples.

In recent years, nanozymes have attracted particular interest and attention as catalysts because of their high catalytic efficiency and stability compared with natural enzymes, whereas how to use simple methods to further improve the catalytic activity of nanozymes is still challenging. In this work, we report a trimetallic metal-organic framework (MOF) based on Fe, Co and Ni, which was prepared by replacing partial original Fe nodes of the Fe-MOF with Co and Ni nodes. The obtained FeCoNi-MOF shows both oxidase-like activity and peroxidase-like activity. FeCoNi-MOF can not only oxidize the chromogenic substrate 3,3,5,5-tetramethylbenzidine (TMB) to its blue oxidation product oxTMB directly, but also catalyze the activation of H2O2 to oxidize the TMB. Compared with corresponding monometallic/bimetallic MOFs, the FeCoNi-MOF with equimolar metals hereby prepared exhibited higher peroxidase-like activity, faster colorimetric reaction speed (1.26-2.57 folds), shorter reaction time (20 min) and stronger affinity with TMB (2.50-5.89 folds) and H2O2 (1.73-3.94 folds), owing to the splendid synergistic electron transfer effect between Fe, Co and Ni. Considering its outstanding advantages, a promising FeCoNi-MOF-based sensing platform has been designated for the colorimetric detection of the biomarker H2O2 and environmental pollutant TP, and lower limits of detection (LODs) (1.75 μM for H2O2 and 0.045 μM for TP) and wider linear ranges (6-800 μM for H2O2 and 0.5-80 μM for TP) were obtained. In addition, the newly constructed colorimetric platform for TP has been applied successfully for the determination of TP in real water samples with average recoveries ranging from 94.6% to 112.1%. Finally, the colorimetric sensing platform based on FeCoNi-MOF is converted to a cost-effective paper strip sensor, which renders the detection of TP more rapid and convenient.

Co3O4 hollow nanoparticles embedded in carbon nanoboxes as peroxidase-like nanozymes for the colorimetric determination of H2O2 and dopamine

Nanozymes are interesting alternatives to natural enzymes since they are highly stable and inexpensive. Here, we present a straightforward MOF-based etch, pyrolysis, and oxidation approach for fabricating an attractive hybrid structure comprised of extensively distributed ultrafine Co3O4 hollow particles embedded in carbon nanoboxes (denoted as HP-Co3O4@C NBs), which exhibit the catalytic property of peroxidase-like nanozyme. The ultrafine size and hollow structure of Co3O4 particles provide a large surface area and abundant catalytic sites of HP-Co3O4@C NBs. Mechanistic studies show that the production of reactive oxygen species (ROS) intermediates (•OH) through the decomposition of H2O2 endows the peroxidase-like activity of HP-Co3O4@C NBs. 3, 3′, 5, 5′-tetramethylbenzidine (TMB) transforms into a blue oxidation product (oxTMB) in the presence of H2O2 with the catalysis of HP-Co3O4@C NBs. In the existence of the reducing substance dopamine (DA), the oxidation reaction of TMB is inhibited, resulting in a marked bleaching of the blue colour. Based on these principles, we develop a colourimetric sensing platform for detecting DA with excellent sensitivity and stability. This study not only extends the application of Co-MOFs in the analytical field, but also achieves a simple, rapid (3 min), sensitive, and intuitive detection of H2O2 and dopamine, which is potentially promising for real-time monitoring of biological small molecules.

Exploring the Impact of Ethnocentrism, and Country of Origin on Consumer Purchase Intentions: A Case Jingdezhen Blue-and-White Ceramics

In China, the native people living in the society have great enthusiasm for their country and they always prefer using and consuming their homeland-manufactured products. Moreover, Chinese have their own culture in which they possess different arts and creative commodities consumption and purchasing to follow their traditional norms. This study investigates the patriotic nature of Chinese customers, who have a strong preference for their home-made products and cultural heritage. The research uses an empirical model to examine the relationship between animosity, ethnocentrism, country of origin, and country knowledge, as well as perceived warmth as a mediator. The study used a positivist philosophy with a deductive approach, using the S-O-R theory as the empirical support. Quantitative primary data was collected from 280 customers of blue and white ceramic products, with a total sample size of 280. The SEM technique revealed that country of origin and ethnocentrism significantly influences customer purchase intentions for ceramic products. Furthermore, the mediation analysis revealed that customer ethnocentrism indirectly impacts purchase intentions through perceived warmth. The study concludes that Chinese customers have a high devotion to their mother country's products, and the behavior of native sellers and general society people is crucial in stimulating their purchase intentions.

Modulating Visible-Light Driven NIR Lanthanide Polymer Photocatalysis for Amplification Detection of Exosomal Proteins and Cancer Diagnosis.

Near-infrared (NIR) luminescent lanthanide materials hold great promise for bioanalysis, as they have anti-interference properties. The approach of efficient luminescence is sensitization through a reasonable chromophore to overcome the obstacle of the aqueous phase. The involvement of the surfactant motif is an innovative strategy to arrange the amphiphilic groups to be regularly distributed near the polymer to form a closed sensitized space. Herein, a lanthanide polymer (TCPP-PEI70K-FITC@Yb/SDBS) is designed in which the meso-tetra(4-carboxyphenyl)porphine (TCPP) ligand serves as both a sensitizer and photocatalytic switch. The surfactant sodium dodecyl benzenesulfonate (SDBS) wraps the photosensitive polymers to form a hydrophobic layer, which augments the light-harvesting ability and expedites its photocatalysis. TCPP-PEI70K-FITC@Yb/SDBS is subsequently applied as an amplified photocatalysis toolbox for universally regulating the generation of reactive oxygen species (ROS). Boosting 3,3',5,5'-tetramethylbenzidine (TMB) oxidation to produce blue products, a dual-mode biosensor is fabricated for improving the diagnosis of programmed death ligand-1-positive (PDL1) cancer exosomes. Exosomes were captured by Fe3O4 modified by the PDL1 aptamer, enabling replacement of alkaline phosphatase (ALP)-labeled multiple hybridized chains; then, the isolated ALP triggered a hydrolysis reaction to block the generation of oxTMB. Detection sensitivity improves by 1 order of magnitude through SDBS modulation, down to 104 particles/mL. The sensor performed well clinically in distinguishing cancer patients from healthy individuals, expanding physiological applications of near-infrared lanthanide luminescence.

Smartphone assisted paper strip-based colorimetric sensing of phosphate and copper ions utilizing bi-ligand intercalated cobalt-MOF as a dual functional nanozyme

Monitoring phosphate and copper ions is vital for environmental and food safety due to associated health risks. To address this need, a new cobalt and bi-organic ligand based metal organic framework (BDC-Co-BIm MOF) has been developed. This MOF acts as a dual-function nanozyme, displaying peroxidase-like activity and selective detection abilities for PO43- ion. The Co(II) nodes and sp2 carbon within the framework exhibit excellent Fenton-like reaction, efficiently catalyzing the conversion of 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidised TMB (oxTMB). Also, steady-state kinetic analysis indicated that BDC-Co-BIm MOF exhibited high affinity toward both TMB and H2O2. The Co(II) nodes and positive charge of the BDC-Co-BIm MOF capture PO43- ion through Co-O-P bonding and electrostatic interaction, limiting the electron transfer between BDC-Co-BIm MOF and H2O2. This reduces TMB oxidation and changes the color from blue to colorless. When ionic Cu2+ ion are added to the system, they react with PO43- ion to form a stable Cu3(PO4)2 complex. This reactivates the electron transfer process, allowing the H2O2 to decompose into •OH. As a result, TMB oxidation is restored and producing the blue color product again. Based on these principles, a colorimetric assay has been developed for the rapid and selective detection of PO43- and Cu2+ ions. A strong linear correlation has been established between their concentrations and the UV absorbance at 652 nm, with limit of detection (LOD) of PO43- and Cu2+ was 0.05 and 0.13 µM, respectively. These limits are notably lower than the thresholds established by the Environmental Protection Agency and World Health Organization (EPA & WHO) at 0.18 µM for PO43- and 30 µM for Cu2+. A Paper-based smartphone-assisted sensing of PO43- and Cu2+ ions was successfully achieved using intelligent Red, Green, and Blue (RGB) analysis. The platform showed high selectivity and performance with environmental water samples. This research presents a novel method for visually detecting PO43- and Cu2+ ions using versatile nanozyme, advancing portable on-site environmental safety analysis.

형상 문법에 기반한 징더전 청화백자 문화상품 디자인

형상 문법에 기반한 징더전 청화백자 문화상품 디자인

MXene/Carbon Dots Nanozyme Composites for Glutathione Detection and Tumor Therapy.

Co-N-CDs-based MXene nanocomposites (MXene@PDA/Co-N-CDs) were constructed by decorating Co-N-CDs on polydopamine-functionalized MXene nanosheets. Both Co-N-CDs and MXene nanosheets have peroxidase-like activity; when the two materials are combined to form MXene@PDA/Co-N-CDs nanocomposites, the peroxide-like activity can be further enhanced. MXene@PDA/Co-N-CDs could oxidize the substrate 3,3'5,5'-tetramethylbenziline (TMB) to form ox-TMB, as confirmed by detecting the absorption of the blue products. A highly selective colorimetric biosensor was developed for the determination of glutathione (GSH) in the concentration range of 0.3 to 20 µM with a lower detection limit (LOD) of 0.12 µM, which realized the accurate detection of GSH in human serum and urine samples. Moreover, in the tumor microenvironment, MXene@PDA/Co-N-CDs could catalyze hydrogen peroxide to produce hydroxyl free radicals and produce a photothermal effect under the exposure of NIR-I irradiation. The catalytic activity of MXene@PDA/Co-N-CD nanocomposites was fully achieved for the death of cancer cells through photothermal/photodynamic synergistic therapy. The MXene@PDA/Co-N-CDs nanozyme offers multiple applications in GSH detection and tumor therapy.

Raspberry-shaped ZIF-8/Au nanozymes with excellent peroxidase-like activity for simple and visual detection of glutathione.

Artificial enzymes with high stability, adjustable catalytic activity, controllable preparation, and good reproducibility have been widely studied. Noble metal nanozymes, particularly gold nanoparticles (Au NPs), exhibit good catalytic activity, but their stability is poor. In this study, zeolitic imidazolate framework-8 (ZIF-8) was used as a carrier for Au NPs, thus improving the utilization efficiency and conservation stability of the nanozymes. A ZIF-8/Au nanocomposite with peroxidase activity and a raspberry-shaped structure was synthesized. In the assay, ZIF-8/Au catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to a blue product oxidized TMB (oxTMB). Glutathione (GSH) selectively inhibited this reaction, with a detection limit of 0.28µM and linear range of 0.5-60µM. Using the photo and chromaticity analysis functions, we developed a portable analysis method using a smartphone equipped with a camera module as a detection terminal for a wide range of rapid screening techniques for GSH. Preparation of raspberry-shaped ZIF-8/Au improved the catalytic activity of Au NPs and good results were demonstrated in serum, which suggests their promising application under physiological conditions.