Blood Serum Lipoproteins Research Articles

Association between sialic acid, sialidase activity and cholesterol of lipid-containing circulating immune complexes in the blood serum of patients with atherosclerosis

Atherosclerosis is the most common chronic non-infectious diseases, in the pathogenesis of which the accumulation of lipids in the subendothelial layer of the arteries and the local inflammatory reaction play a significant role. The source of lipid accumulation in the vascular wall is modified low-density lipoproteins. Desialylation is one of the known modifications that leads to the emergence of atherogenic properties in low-density lipoproteins. Enzymes that have sialidase activity circulate in human blood, i.e., the ability to cleave sialic acid from low-density lipoproteins. Desialylated low-density lipoproteins are autoantigens and induce the production of IgG autoantibodies, which form immune complexes with low-density lipoproteins, which aggravates the course of atherosclerosis. The purpose of the study was to establish associations between the levels of sialic acid in low-density lipoproteins, sialidase activity and the cholesterol content of lipid-containing circulating immune complexes in the blood serum of patients with atherosclerosis. Blood sera from patients with coronary heart disease were used as biological material to determine indicators of sialidase activity, cholesterol content of lipid-containing circulating immune complexes and sialic acid in low-density lipoproteins, which were isolated from blood serum. Serum samples were obtained from the laboratory of clinical biochemistry of the Institute of Clinical Cardiology, A.L. Myasnikov Federal State Budgetary Institution National Medical Research Center of Cardiology named after Academician E.I. Chazov as unutilized residues after performing routine biochemical tests. Fifty-one samples of blood serum and low-density lipoproteins isolated from it were analyzed. A significant positive relationship was revealed between the cholesterol content of circulating immune complexes and sialidase activity in the blood serum (r = 0.305 at p = 0.029). At the same time, no correlation was found between the content of sialic acid in low-density lipoproteins and sialidase activity in the blood serum, as well as between the cholesterol content of circulating immune complexes in the blood serum and sialic acid in low-density lipoproteins. It should be assumed that increased sialidase activity in the blood serum leads to the formation of desialylated immunogenic low-density lipoproteins with the subsequent appearance of autoantibodies and the formation of lipid-containing circulating immune complexes.

Enhanced hepatic metabolic perturbation of polystyrene nanoplastics by UV irradiation-induced hydroxyl radical generation

The environmental behavior of and risks associated with nanoplastics (NPs) have attracted considerable attention. However, compared to pristine NPs, environmental factors such as ultraviolet (UV) irradiation that lead to changes in the toxicity of NPs have rarely been studied. We evaluated the changes in morphology and physicochemical properties of polystyrene (PS) NPs before and after UV irradiation, and compared their hepatotoxicity in mice. The results showed that UV irradiation caused particle size reduction and increased the carbonyl index (CI) and negative charge on the particle surface. UV-aged PS NPs (aPS NPs) could induce the generation of hydroxyl radicals (·OH), but also further promoted the generation of ·OH in the Fenton reaction system. Hepatic pathological damage was more severe in mice exposed to aPS NPs, accompanied by a large number of vacuoles and hepatocyte balloon-like changes and more marked perturbations in blood glucose and serum lipoprotein, alanine aminotransferase and aspartate aminotransferase levels. In addition, exposure to PS NPs and aPS NPs, especially aPS NPs, triggered oxidative stress and significantly damaged the antioxidant capacity of mice liver. Compared with PS NPs, exposure to aPS NPs increased the number of altered metabolites in hepatic and corresponding metabolic pathways, especially glutathione metabolism. Our research suggests that UV irradiation can disrupt the redox balance in organisms by promoting the production of ·OH, enhancing PS NPs-induced liver damage and metabolic disorders. This study will help us understand the health risks of NPs and to avoid underestimation of the risks of NPs in nature.

Positive effects of Quercetin, Iron Oxide Nanoparticles and Ascorbic Acid composition in the treatment of women with metabolic syndrome

Relevance of study. Metabolic syndrome (MS) increases the risk of atherosclerotic cardiovascular disease and type ІІ diabetes. Correction of the MS manifestations is important for reducing cardiovascular risk. Aim - to study the effects of using a composition consisting of quercetin (Q), polyvalent iron oxide nanoparticles (IONs) and ascorbic acid (AA) in women with MS. Materials and methods. 60 women with MS took part in the study. They were divided equally into two groups. The control group patients received a placebo for one month, and the main group received a composition of Q, IONs and AA for one month too. Anthropometric parameters, fasting plasma glucose level and standard oral glucose tolerance test (OGTT), serum lipid concentration, and microvascular endothelial function in a sample with reactive post-occlusion hyperemia were determined. Results. The use of the composition led to a decrease in the glycemia level of both on an empty stomach and after 2 hours of SGTT, the concentration of total cholesterol and low-density lipoproteins in blood serum in atherogenic dyslipidemia patients. At the same time, there was an improvement in the endothelium functional state of the microvessels, as evidenced by an increase in the maximum volume velocity of skin blood flow in the reactive hyperemia test. Conclusion. It was found that the use of a composition Q, IONs and AA had a positive effect on cardiovascular risk factors in MS patients. _________________________________________________________________________________________ Keywords: iron oxide nanoparticles; quercetin; metabolic syndrome; glucose and blood lipids; the microvascular endothelium function.

Beneficial Effect of Cuban Policosanol on Blood Pressure and Serum Lipoproteins Accompanied with Lowered Glycated Hemoglobin and Enhanced High-Density Lipoprotein Functionalities in a Randomized, Placebo-Controlled, and Double-Blinded Trial with Healthy Japanese

This study evaluated the efficacy and safety of 20 mg of Cuban policosanol in blood pressure (BP) and lipid/lipoprotein parameters of healthy Japanese subjects via a placebo-controlled, randomized, and double-blinded human trial. After 12 weeks of consumption, the policosanol group showed significantly lower BP, glycated hemoglobin (HbA1c), and blood urea nitrogen (BUN) levels. The policosanol group also showed lower aspartate aminotransferase (AST), alanine aminotransferase (ALT), and γ-glutamyl transferase (γ-GTP) levels at week 12 than those at week 0: A decrease of up to 9% (p < 0.05), 17% (p < 0.05), and 15% (p < 0.05) was observed, respectively. The policosanol group showed significantly higher HDL-C level and HDL-C/TC (%), approximately 9.5% (p < 0.001) and 7.2% (p = 0.003), respectively, than the placebo group and a difference in the point of time and group interaction (p < 0.001). In lipoprotein analysis, the policosanol group showed a decrease in oxidation and glycation extent in VLDL and LDL with an improvement of particle shape and morphology after 12 weeks. HDL from the policosanol group showed in vitro stronger antioxidant and in vivo anti-inflammatory abilities. In conclusion, 12 weeks of Cuban policosanolconsumption in Japanese subjects showed significant improvement in blood pressure, lipid profiles, hepatic functions, and HbA1c with enhancement of HDL functionalities.

Changes in Lipoprotein Particles in the Blood Serum of Patients with Lichen Planus.

Lichen planus is a chronic inflammatory mucocutaneous disease that belongs to the group of papulosquamous skin diseases among diseases like psoriasis, a widely studied disease in dermatology. The aim of the study was to identify the changes between the blood sera of lichen planus patients and healthy controls to widen the knowledge about the metabolomic aspect of lichen planus and gain a better understanding about the pathophysiology of the disease. We used high-throughput nuclear magnetic resonance (NMR) spectroscopy to measure the levels of blood serum metabolites, lipoproteins and lipoprotein particles. Dyslipidemia has relatively recently been shown to be one of the comorbidities of lichen planus, but the changes in the components of lipoproteins have not been described yet. We found statistically significant changes in the concentrations of 16 markers regarding lipoproteins, which included the components of intermediate-density lipoproteins, low-density lipoproteins and large low-density lipoproteins. We propose that the detected changes may increase the risk for specific comorbidities (e.g., dyslipidemia) and resulting cardiovascular diseases, as the turnover and hepatic uptake of the altered/modified lipoprotein particles are disturbed.

Pharmacotherapy optimization opportunities using fixed combinations in patients with high cardiovascular risk

This review focuses on the role of arterial hypertension and hyperlipidemia as the most important risk factors of early disability and death. The facts are provided that many persons with those risk factors do not have the adequate control of blood pressure and atherogenic fractions of blood serum lipoproteins. The review addresses prospects for optimizing pharmacotherapy with fixed combinations of drugs for arterial hypertension and dyslipidemia. From the perspective of effective clinical guidelines and the available evidence base, the authors reviewed possibilities for improving the treatment compliance by using combinations of antihypertensive and hypolipidemic drugs in a single dosage form. Implementation of such complex, optimized treatment using a three-component fixed-dose combination is considered.

Analysis of the SYSDIET Healthy Nordic Diet randomized trial based on metabolic profiling reveal beneficial effects on glucose metabolism and blood lipids

Intake assessment in multicenter trials is challenging, yet important for accurate outcome evaluation. The present study aimed to characterize a multicenter randomized controlled trial with a healthy Nordic diet (HND) compared to a Control diet (CD) by plasma and urine metabolic profiles and to associate them with cardiometabolic markers. During 18-24 weeks of intervention, 200 participants with metabolic syndrome were advised at six centres to eat either HND (e.g. whole-grain products, berries, rapeseed oil, fish and low-fat dairy) or CD while being weight stable. Of these 166/159 completers delivered blood/urine samples. Metabolic profiles of fasting plasma and 24h pooled urine were analysed to identify characteristic diet-related patterns. Principal components analysis (PCA) scores (i.e. PC1 and PC2 scores) were used to test their combined effect on blood glucose response (primary endpoint), serum lipoproteins, triglycerides, and inflammatory markers. The profiles distinguished HND and CD with AUC of 0.96±0.03 and 0.93±0.02 for plasma and urine, respectively, with limited heterogeneity between centers, reflecting markers of key foods. Markers of fish, whole grain and polyunsaturated lipids characterized HND, while CD was reflected by lipids containing palmitoleic acid. The PC1 scores of plasma metabolites characterizing the intervention is associated with HDL (β=0.05; 95% CI: 0.02, 0.08; P=0.001) and triglycerides (β=-0.06; 95% CI:-0.09,-0.03; P<0.001). PC2 scores were related with glucose metabolism (2h Glucose, β=0.1; 95% CI: 0.05, 0.15; P<0.001), LDL (β=0.06; 95% CI: 0.01, 0.1; P=0.02) and triglycerides (β=0.11; 95% CI: 0.06, 0.15; P<0.001). For urine, the scores were related with LDL cholesterol. Plasma and urine metabolite profiles from SYSDIET reflected good compliance with dietary recommendations across the region. The scores of metabolites characterizing the diets associated with outcomes related with cardio-metabolic risk. Our analysis therefore offers a novel way to approach a per protocol analysis with a balanced compliance assessment in larger multicentre dietary trials. The study was registered at clinicaltrials.gov with NCT00992641.

Evaluation of α-klotho, FGF-23 and Insulin Resistance in Obese Prediabetics

Aim: Prediabetes is a condition with hyperglycemia and risk of development type 2 diabetes mellitus (T2DM). The aim of this study is to determine the concentrations of blood pressure, serum lipoproteins, glucose and insulin levels as well as the concentrations of α-klotho and FGF-23 parameters. We also aim at the correlation between all these parameters in obese prediabetics and obese normoglycemics. Material methods: A total of 26 obese normoglycemic (control group) and 25 obese prediabetic individuals were included in this study. Glucose, haemoglobin A1c (HbA1c) and cholesterols levels were analyzed as part of a routine procedure in the biochemistry laboratories of our hospital using an autoanalyzer. In order to determine α-klotho and fibroblast growth factor-23 (FGF-23) levels, ELISA method was used. Result: According to the findings, a significant increase was detected in serum LDL-C, TC, HbA1c, glucose, insulin and HOMA-IR levels in the obese prediabetic group compared to the control group (p <0.05). However, serum α-klotho and FGF-23 levels were found to be significantly lower in the obese prediabetic group compared to the control group (p <0.05). α-klotho and FGF-23 values showed strong negative correlation when compared with HOMA-IR values separately (p <0.01). Conclusion: These findings suggest that α-klotho and / or FGF-23 parameters may be a marker for prediabetes. The results of our study, together with the future studies, will make a significant contribution to the literature, as prediabetes diagnostic criteria in the diabetes outpatient clinics.

Влияние интоксикации сероуглеродом на биохимические показатели крови и их коррекция растительными фенольными соединениями в эксперименте

Introduction. Phenolic compounds that are part of a variety of phytopreparations have an anti-radical and antioxidant effect. Aim. Studying the influence of carbon disulfide intoxication on the rat blood biochemical parameters and the possibility of their correction with natural plant phenolic compounds from viburnum. Materials and methods. The experiment was conducted on white Wistar male rats. Carbon disulfide intoxication (concentration 2.0 mg/m3) was implemented in a special exposure chamber. The animals were divided into 5 groups: the 1st group was control (intact rats); the 2nd group was carbon disulfide intoxication for 3 weeks; the 3rd group was carbon disulfide intoxication for 3 weeks, followed by discontinuation for 7 days; the 4th group was carbon disulfide intoxication for 3 weeks, followed by administration of the viburnum extract for 7 days; the 5th group was carbon disulfide intoxication for 3 weeks, followed by silymarin administration for 7 days. The drugs were administered at a dose of 100 mg of total phenols per kilogram of animal weight. Results. Carbon disulfide intoxication was accompanied by the development of intense hypercholesterolemia; an increase in the fraction of low-density lipoproteins with simultaneous decrease in high-density lipoproteins in blood serum. There was an increase in the level of malondialdehyde, decrease in the superoxide dismutase activity and reduced glutathione amount, which indicates the depletion of the body’s antioxidant defense system. During the withdrawal period of carbon disulfide intoxication for 7 days, the blood biochemical parameters did not recover, which indicates the preservation of free-radical processes. The administration of the viburnum extract and the comparison drug, silymarin, was accompanied by a recovery to the control group level of blood biochemical parameters after carbon disulfide intoxication. Conclusion. The toxic effect of carbon disulfide causes a change in the studied blood biochemical parameters. The administration of the viburnum extract and silymarin was accompanied by their restoration to the level of the control group, but a more pronounced effect was observed in the viburnum extract due to the presence of oligomeric forms of polyphenols in its composition.

Associations of diet quality and blood serum lipoprotein levels in a population at high risk for diabetes: the Strong Heart Family Study.

Background/ObjectivesPrevious studies consistently report that diet quality is inversely associated with risk of cardiovascular disease (CVD) and type 2 diabetes. However, few studies have assessed the association of diet quality with serum lipoproteins, an intermediate-marker of cardio-metabolic health, or assessed whether type 2 diabetes modifies these associations. This study assessed associations of diet quality (evaluated using the Alternative Healthy Eating Index (AHEI)), and the interaction of diet quality with diabetes, on total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C), apolipoprotein A (apoA1) and apolipoprotein B (apoB) among American Indians (AIs).Subjects/MethodsParticipants comprised AIs who participated in the Strong Heart Family Study (SHFS)--a study of CVD and its risk factors in 12 AI communities. Generalized estimated equations (GEE) were used to examine the: (1) cross-sectional associations of diet quality (as determined by AHEI) with serum lipoproteins (n=2 200); and (2) the prospective associations of the AHEI measured at baseline with serum lipoproteins (n=1 899).ResultsThe association of AHEI with TC, LDL-C, and ApoB differed according to diabetes status. In prospective analysis, AHEI was associated with more favorable levels of TC (p=0.029) and LDL-C (p=0.008) among participants with diabetes independent of other demographic, behavioral, and health factors. The association of diet quality with TC, LDL-C, and ApoB were much weaker among participants without diabetes. There was no association of diet quality with TG, HDL-C, or ApoA.ConclusionsThe associations of diet quality with TC, LDL-C, and ApoB differ according to diabetes status.

Canine visceral leishmaniosis: the relationship of blood serum thyroid hormones, lipids, and lipoproteins with clinical status.

The objective of the present study was to investigate the role of thyroid hormones and lipid profile in development and outcome of canine visceral leishmaniosis (CVL). We therefore studied the relationships between blood serum thyroid hormones [total T4, free T4, total T3, and free T3], lipids, and lipoproteins [total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C)] and clinical status in dogs naturally infected with Leishmania infantum. Two groups of Leishmania-infected dogs [with no clinical signs (NCS; n = 15), and with clinical signs (CS; n = 16)] were assessed and compared with a group of healthy control dogs (n = 15). A significant decrease in serum total T4 (p < 0.05) concentration in the CS group was observed when compared to the NCS and control groups. The dogs in CS group revealed a significant decrease (p < 0.05) in free T4 concentration in comparison to the control group. The CS group presented a significant decrease in HDL-C (p < 0.01) concentrations, when compared to NCS and control groups. The observed findings in the present study suggested that thyroid hormone and lipoprotein alterations may have a role in susceptibility of dogs with Leishmania infection.

THE EFFECT OF AEROBIC EXERCISE AND WEIGHT-LIFTING PLUS AEROBIC EXERCISE ON BLOOD PRESSURE AND BLOOD PARAMETERS IN SEDENTARY FEMALES

The purpose of this study was determined the effects of 12 weeks aerobic exercise and weight-lifting plus aerobic exercise on blood pressure, blood serum lipids and lipoproteins of sedentary females. Thirty seven sedentary females (Aerobic group mean age: 44.66 years, Weight-Lifting + Aerobic group mean age: 40.80 years) participated in this study. Before initiate the exercise their blood samples, peripheral measurements and thickness of skinfolds were taken. The subjects participated in aerobics group (n = 21) and weight-lifting plus aerobics group (n = 16) 3 times in a week through one hour. The intensity of exercise was increased gradually and their heart rates were raised up to 130-140 rates per-minute and of the tension. Two way ANOVA was used in this study. There were no significant difference between two different type of exercise groups (p>0.05) first group (aerobic); HDL-C (High Density Lipoprotein-Cholesterol) 18.12%, second group (weight-lifting plus aerobic) %28.23, there were a significant increase among them (p<0.01). Total cholesterol decrease 13.97% in group of aerobic exercise and it decreases 7.13% in group of weight-lifting plus aerobic. Triglyceride decreases 31.74% in group of aerobic exercise; it decreases 19.53% in group of weight-lifting plus aerobic exercise. Level of LDL-C was decreased 28.11% in group of aerobic exercise, 21.46% in group of weight-lifting plus aerobic. When we consider exercise types, the only flats of type is on triglyceride and APO B, (p<0.05), there were effects of exercise duration is on HDL-C, LDL-C AND Total cholesterol (p<0.01). There were effects of type and duration of exercise on TC, TG, HDL-C, LDL-C, APO A1, and APO B (P<0.01). In this study, regular aerobic and weight-lifting plus aerobic exercises have been shown to reduce the risk of cardiovascular disease in sedentary women and a significant improvement in systolic and diastolic blood pressures as well as reduction of cholesterol, triglyceride, and of LDL-C. Level of HDL-C was increased in both of exercise groups. Article visualizations:

Development of liposomal form of polyunsaturated fatty acid concentrate: ways of using in production of functional foods

The research is aimed at obtaining a concentrate of polyunsaturated fatty acids (PUFA) in liposomal form and developing possible ways of PUFA application in manufacturing functional food products. The PUFA concentrate was obtained from the fat of the Baikal seal by method of complexing with urea. We prepared a liposomal form of PUFA concentrate by evaporating organic solvent from the solution of lipids to form a thin lipid film with addition of buffer solution and further extrusion of the obtained emulsion. We defined the size of liposomal particles by method of turbidimetry. We identified the fatty acid composition of the samples by gas-liquid chromatography using a chromatographymass spectrometer. Biomedical research was conducted on 30 adult male Wistar rats with initial weight of 130-150 g. We induced atherogenic experimental hyperlipemia in rats by adding 5% cholesterol, 0.3% of 6-methyl-2-thiouracil, 1% cholic acid and 5% of lard to their normal diet for 21 days. We used an automatic biochemical analyzer to define total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoproteins (VLDL) in blood serum. As a result of the experiment, we obtained a concentrate from the seal fat with content of essential PUFA 12.2% of total fatty acids in which the ratio of ω-6:ω-3 was 1.3:1. We obtained the liposomal form of the PUFA concentrate, with the average size of the liposomes ranging from 144 to 158 nm. We have established that the blood serum of the animals with induced hyperlipidemia, which orally took the liposomal form of PUFA concentrate for 14 days in a dose of 20 mg/kg body weight, showed the decrease of total cholesterol content of LDL and VLDL, while HDL increased to the parameters in intact animals. This indicated a normalization of the lipid profile in rats and the lipid-lowering effect of the concentrate. Also, we have studied the possibility of enriching wheat bread with essential fatty acids by replacing some part of the water with the liposomal form of PUFA concentrate. The use of this additive increased the output volume of bread by 14%, intensified the crust color and didn't affect the organoleptic indicators of bread quality. Fatty acid analysis of the lipids in bread showed that after adding 3 g of PUFA concentrate in liposome form per 100 g of flour increased the content of ω-3 PUFA by 3.2 fold (191 mg per 100 g of bread), with the ratio of ω-6:ω-3 PUFA being 5.8:1. Thus, bread enriched with PUFA concentrate in liposome form can be positioned as a functional food product that contains ω-3 PUFA in an amount of 19.1% of the recommended daily intake in 100 g of final product and also as a product with optimal PUFA ratio of ω-6:ω-3.

AQUEOUS EXTRACT OF RED TEA PLANT (Hibiscus sabdariffa L) EFFECT ON THE LIPID PROFILE AND GLUGOSE IN MALE WHITE RABBITS EXPOSED TO OXIDATIVE STRESS

This study aimed to investigate the of effect aqueous extract of red tea on average of glucose and lipid profile in New Zealand white male rabbits exposed to oxidative stress induced hydrogen peroxide H2O2 of drinking water for the duration 30-days. Treatment of hydrogen peroxide 2% led alone to a significant increase (P≤0.05) in the concentration of glucose level and the concentration of total cholesterol rate and level of triglycerides and a significant decrease (P ≤ 0.05) in the level of estimating the concentration of high-density cholesterol in the blood serum lipoproteins and a significant increase in the concentration of lipoproteins and very low-lying density cholesterol was significantly higher in atherogenic indices the first and second, respectively, in the blood of animals treated with hydrogen peroxide compared to the control group. It got a significant decrease (P≤0.05) in the concentration of glucose and cholesterol rate and triglycerides and in the concentration of lipoproteins and sessile very density cholesterol and in the atherogenic indices first and second respectively, as happened morally rise in the level of estimating the concentration of high-density lipoproteins cholesterol in the labor animal blood serum aqueous extract of red tea (125 mg / kg of body weight) added all of the hydrogen peroxide compared with the group treated rabbits hydrogen peroxide alone. It is concluded from this current study, the important role of the Red tea as an antioxidant through curb the harmful effects of some types of free radicals within the body and thus improve the most changes biochemical changes in the male albino rabbits after exposure to oxidative stress Induced by hydrogen Peroxide 2% .

Lipids and lipoproteins and inflammatory markers in patients with chronic apical periodontitis.

BackgroundSince chronic apical periodontitis (CAP) appears to be a risk factor for coronary heart disease, the aim of the study was to determine the relationship between the size of CAP lesion and inflammatory markers (hsCRP, IL-6, TNF-α), as well as lipids and lipoproteins (LpPLA2, apoAI, apoB level) in blood serum of patients with CAP.MethodsThe patients studied (n = 43) were divided into groups: patients under 50 and over 50 years of age, and a separate subgroup of the oldest age with the largest size of CAP lesions. Apolipoprotein AI (apoAI) above 150 mg/dL and below 150 mg/dL was used as an important criterion for the division of patients into groups. The CAP lesion size was measured using the Kodak digital imaging system software. The control group consisted of clinically healthy volunteers (n = 20) without CAP. Lipids were measured on a Siemens analyzer (Germany), apoAI, apoB, hsCRP levels were determined by immunonephelometric method, using the Health Care Diagnostic Product (Siemens GmbH, Germany), and IL-6, TNF-α and LpPLAG7 assay kits (ELISA, R&D Systems) were used.ResultsThe findings suggested that in patients with CAP and their age increase, the CAP lesion size, the concentration of inflammatory markers and LpPLA2 mass increased. Correlations between the CAP lesion size and LpPLA2 mass and between the CAP lesion size and TG level in patients with apoAI 150 ≤ mg/dL showed increase TG in atherogenic apoB-containing triglyceride-rich lipoprotein and TC in cholesterol-rich lipoprotein. The patients with a low apoAI and high LpPLA2 level can have a higher risk of odontogenic disease and progression of atherosclerosis and coronary heart disease.ConclusionWe have found a positive correlation between apoAI level and the CAP lesion size and a negative correlation between LpPLA2 level and the CAP lesion size. The results suggest that apoAI and LpPLA2 in HDL particles have antiinflammatory action and together can limit the CAP lesion size in patient with a higher apoAI level. The literature data on the distribution of lipoprotein particles in subjects are still insufficient, so this problem requires further studies.

Physiological Properties of Dietary Ellagitannin-Rich Preparations Obtained from Strawberry Pomace Using Different Extraction Methods

Abstract The objective of this study was to establish the composition of strawberry preparations rich in ellagitannins obtained using water or acetone extraction (EF and EP preparation, respectively). Then, biological effect of these extracts was assessed in 4-wk nutritional experiment on Wistar rats. The preparations were applied in cholesterol-containing diets that had equal content of ellagitannins (0.03%). To measure animals response, parameters describing the caecal fermentation (ammonia and short-chain fatty acid concentrations, bacterial enzymes activity), blood serum lipoprotein profile, and TBARS content in selected tissues (heart, liver, kidney) were assessed. Apart from polyphenols, including ellagitannins (7.8 and 7.1%, respectively), the EF preparation contained high quantities of soluble dietary fibre and other carbohydrates (33.3 and 38.9%, respectively), whereas the EP preparation was characterised by 58.9% content of ellagitannins, no dietary fibre and a high content of proanthocyanidins (16.9%). In comparison to EF group, the dietary treatment with EP had a stronger effect on caecal environment as manifested by decreased digesta bulk, β-glucuronidase activity and total short-chain fatty acid concentration (P&lt;0.05 vs. group C without supplementation). Both preparations lowered lipaemia and glycaemia. It could be concluded that more efficient acetone extraction of strawberry pomace increased the content of both ellagitannins and proanthocyanidins in the polyphenolic preparation, which caused a stronger inhibiting effect on caecal fermentation processes and at the same time lowered blood triacylglycerols and glucose level. Considering the equal content of ellagitannins in both supplemented diets, it may be speculated that the above effects were due to the presence of proanthocyanidin fraction.

Community-Based Mind–Body Meditative Tai Chi Program and Its Effects on Improvement of Blood Pressure, Weight, Renal Function, Serum Lipoprotein, and Quality of Life in Chinese Adults With Hypertension

Obesity, metabolic syndrome, dyslipidemia, and poor quality of life are common conditions associated with hypertension, and incidence of hypertension is age dependent. However, an effective program to prevent hypertension and to improve biomedical factors and quality of life has not been adequately examined or evaluated in Chinese older adults. This study aims to examine the effectiveness of a Tai Chi program to improve health status in participants with hypertension and its related risk factors such as dyslipidemia, hyperglycemia, and quality of life in older adults in China. A randomized study design was used. At the conclusion of the intervention, 266 patients remained in the study. Blood pressure and biomedical factors were measured according to the World Diabetes Association standard 2002. A standardized quality-of-life measure was used to measure health-related quality of life. It was found that a Tai Chi program to improve hypertension in older adults is effective in reducing blood pressure and body mass index, maintaining normal renal function, and improving physical health of health-related quality of life. It did not improve existing metabolic syndrome levels, lipid level (dyslipidemia) or fasting glucose level (hyperglycemia), to prevent further deterioration of the biomedical risk factors. In conclusion, Tai Chi is effective in managing a number of risk factors associated with hypertension in Chinese older adults. Future research should examine a combination of Tai Chi and nutritional intervention to further reduce the level of biomedical risks.

Evaluation of Nuvan Toxicity Stress on Blood Lipoprotein of Fresh Water Fish Channa punctatus

Organophosphate insecticide Nuvan (DDVP) has selected for the present study, Lethal concentrations were calculated for the fresh water snake headed fish Channa punctatus i.e 0.27ml/L after 24, 48, 72 and 96 hours. For the chronic study 1/10th of the Nuvan concentration (0.027ml/L) was provided to the observed fishes. Blood serum lipoprotein HDL, VLDL and LDL High Density lipoprotein, Very low Density lipoprotein and low density lipoprotein respectively were estimated after chronic toxic stress of Nuvan to fish Channa punctatus. Fish serum lipoprotein and HDL revealed significant decrease level while LDL and VLDL showed significant elevated level after 7, 14, 21, and 28 days at different level p <0.01 and p <0.001.All values are mentioned in graphs respectively.

Effects of high phenolic olive oil on cardiovascular risk factors: A systematic review and meta-analysis

BackgroundCardiovascular diseases are the world's leading cause of death. Prevention by nutrition is an easy and effective approach especially by advising foods with nutraceutic properties like high phenolic olive oil (HPOO). AimThe aim of this review was to systematically access and meta-analyse the effects of HPOO on risk factors of the cardiovascular system and thusly to evaluate its use as a nutraceutical in prevention. Data synthesisMedline/PubMed, EMBase, the Cochrane Library, CAMbase and CAM-QUEST were searched through July 2013. Randomized controlled trials (RCTs) comparing high vs. low (resp. non) phenolic olive oils in either healthy participants or patients with cardiovascular diseases were included. For study appraisal the Cochrane Collaboration's risk of bias tool was used. Main outcomes were blood pressure, serum lipoproteins and oxidation markers. Standardized mean differences (SMD) and 95% confidence intervals (CI) were calculated and analysed by the generic inverse variance methods using a random effects model. Eight cross over RCTs comparing ingestion (21–90 d) of high vs. low (resp. non) phenolic olive oils with a total of 355 subjects were included. ResultsThere were medium effects for lowering systolic blood pressure (n = 69; SMD −0.52; CI −0.77/−0.27; p < 0.01) and small effects for lowering oxLDL (n = 300; SMD −0.25; CI [−0.50/0.00]; p = 0.05). No effects were found for diastolic blood pressure (n = 69; SMD −0.20; CI −1.01/0.62; p = 0.64); malondialdehyde (n = 71; SMD −0.02; CI [−0.20/0.15]; p = 0.79), total cholesterol (n = 400; SMD −0.05; CI [−0.16/0.05]; p = 0.33); HDL (n = 400; SMD −0.03; CI [−0.14/0.08]; p = 0.62); LDL (n = 400; SMD −0.03; CI [−0.15/0.09]; p = 0.61); and triglycerides (n = 360; SMD 0.02; CI [−0.22/0.25]; p = 0.90). LimitationsThe small number of studies/participants limits this review. ConclusionsHPOO provides small beneficial effects on systolic blood pressure and serum oxidative status (oxLDL). HPOO should be considered as a nutraceutical in cardiovascular prevention.

Effects of coenzyme q10 supplementation on serum lipoproteins, plasma fibrinogen, and blood pressure in patients with hyperlipidemia and myocardial infarction.

Background:Low plasma concentrations of coenzyme Q10 (CoQ10) have been associated with concentration of lipoproteins and other factors contributing to coronary heart diseases.Objectives:The present investigation aimed to improve the blood pressure and serum lipoproteins concentration in patients with myocardial infarction (MI) by CoQ10 supplementation.Patients and Methods:In this randomized double-blinded controlled clinical trial, 52 Iranian patients with hyperlipidemia and MI were recruited to examine the effect of CoQ10 on serum total cholesterol (TC), LDL-C, HDL-C, triglyceride (TG), LDL-C/HDL-C ratio, TC/HDL-C ratio, fibrinogen, systolic blood pressure (SBP) and diastolic blood pressure (DBP). Individuals were randomly allocated to two groups for receiving either 200 mg/d of CoQ10 or placebo for 12 weeks.Results:There were not significant differences in serum LDL-C (2.70 ± 0.31 vs. 2.70 ± 0.35 mmol/L), TC (4.47 ± 0.33 vs. 4.93 ± 0.57 mmol/L), TG (2.48 ± 0.12 vs. 2.25 ± 0.69 mmol/L), and fibrinogen (2.08 ± 0.99 vs. 38.7 ± 0.64 mg/dL) between CoQ10 and placebo groups. After 12 weeks, a significant enhancement in serum HDL-C (1.44 ± 0.18 vs. 1.14 ± 0.18 mmol/L) level was observed between groups after the supplementation (P < 0.001). A significant reduction of TC, LDL-C, and fibrinogen and a significant increase in HDL-C concentration was observed in CoQ10 group after intervention (P < 0.001). Our assessment demonstrated statistically significant differences between the two groups in SBP and DBP after intervention (P < 0.001). ANCOVA also revealed significant differences in the ratio of LDL-C/HDL-C and TC/HDL-C between the two groups (1.89 ± 0.42 vs. 2.39 ± 0.38, P = 0.002; and 3.2 ± 0.5 vs. 4.24 ± 0.66, P = 0.01, respectively). A significant reduction of LDL-C/HDL-C and TC/HDL-C was observed in CoQ10 group (P < 0.001).Conclusions:Twelve-week supplementation with CoQ10 in patients with hyperlipidemia and MI can improve blood pressure, serum HDL-C as well as LDL-C/HDL-C and TC/HDL-C ratios; therefore, it might decrease the risk of frequent MI.