Blood Serotonin Research Articles

Serotonin and Neuropeptides in Blood From Episodic and Chronic Migraine and Cluster Headache Patients in Case-Control and Case-Crossover Settings: A Systematic Review and Meta-Analysis.

The aim of this systematic review and meta-analysis (SR-MA) was to identify signaling molecule profiles and blood-derived biomarkers in migraine and cluster headache (CH) patients. Currently no migraine and CH valid biomarkers are available. Blood tests based on biomarker profiles have been used to gather information about the nervous system. Such tests have not yet been established within the primary headache field. Case-control and case-crossover studies investigating whole blood, plasma, and serum were identified worldwide. The qualitative synthesis focused on 9 signaling molecules (serotonin [5-HT], calcitonin gene-related peptide [CGRP], endothelin-1 [ET-1], neurokinin A, neurokinin B, neuropeptide Y, pituitary adenylate cyclase-activating peptide 38 [PACAP-38], substance P (SP), and vasoactive intestinal peptide) and the quantitative synthesis on 5-HT and CGRP (≥5 comparisons available). The meta-analysis was conducted using standard and 3-level random effect models. Fifty-four eligible studies were identified (87.0% migraine, 9.3% CH, 3.7% migraine, and CH), and 2768 headache patients and 1165 controls included. Comparable fluctuations of 5-HT, CGRP, ET-1, PACAP-38, and SP in blood were generally observed between migraine and CH. Significant findings were observed for some subgroups and strata, for example, higher interictal and ictal 5-HT venous blood levels (ratio of means = 1.32, 95% CI: 1.08; 1.61; ratio of means = 1.23, 95% CI: 1.01; 1.49) in episodic migraine with aura with a female-dominated case group, higher interictal CGRP blood levels in episodic migraine (ratio of means = 1.63, 95% CI: 1.18; 2.26), and chronic migraine (ratio of means = 1.89, 95% CI: 1.33; 2.68), and higher ictal CGRP blood levels (ratio of means = 1.35, 95% CI: 1.09; 1.68) in episodic migraine were observed. In most subgroups, the quantitative synthesis revealed a high degree of heterogeneity between studies in part explained by the blood sampling site, specimen source, blood specimen, and sex distribution. Other potential confounders were age, aura, study quality, menstrual cycle, and methodology (eg, storage temperature). Potential migraine and CH signaling molecule profiles and biomarkers were revealed. Nevertheless, the high degree of heterogeneity between studies impedes identification of valid biomarkers but allowed us to assess the presence of confounders. Consideration of the potential confounders identified in this SR-MA might be of importance in the experimental planning of future studies. This consideration could be incorporated through establishment of specific guidelines.

Free movement: A key for welfare improvement in sport horses?

Horses, and in particular sport horses, remain housed predominantly in single stalls. One of the main reported reasons is the fear that they will become agitated and injure themselves and thereby impair their performance if released in paddocks. The hour spent daily at work is also assumed to be sufficient to satisfy the horses’ needs for locomotion. Growing scientific evidence shows that single stall housing has negative consequences on horses’ welfare and that time for free movement is necessary. Our aim was to assess the feasibility of allowing sport horses used to staying permanently in their stall (except for 1 h riding/day) daily free time in a paddock and to evaluate its potential impact on their welfare using two behavioural reliable indicators (stereotypic behaviours and ear position), as well as selected blood parameters (blood cell count, oxytocin and serotonin concentrations). Two experiments were conducted on the same site. The first experiment evaluated sport horses’ habituation to the novel situation of daily sessions in a paddock, and recorded welfare indicators in their stall before and during the experiment, comparing horses that were taken outdoors (experimental) and those that stayed in their stall (control). The second experiment evaluated the impact of this daily free time in a paddock on horses’ welfare and its durability, focusing on positive indicators. Behavioural observations in paddocks showed that even horses that had never experienced free movement outside their stall habituated rapidly to this situation. The presence of hay in the paddock, may have speeded up habituation. Their restricted living conditions were associated with abnormalities in blood cell count that were not overcome during the time of daily paddock sessions but behavioural indicators showed that their welfare improved. In the second study, the experimental horses’ welfare improved during the paddock release period, in particular their stereotypic behaviours decreased and oxytocin levels increased. No effects on serotonin concentrations could be evidenced. These effects were directly associated with being in paddock, as the indicators returned rapidly to their previous levels indicating compromised welfare when the paddock release sessions stopped. In conclusion, it can be recommended to release sport horses for free movement in paddocks as welfare is improved and subjective assessment by caretakers indicated minimal risks.

Functional polymorphism of the serotonin reuptake transporter SLC6A4 gene in various clinical variants of irritable bowel syndrome

Rationale:Irritable bowel syndrome (IBS) is a multifactorial disease, the genetic aspect of which is being actively studied.Aim:To investigate functional polymorphism of the serotonin reuptake transporter (SERT)SLC6A4gene of various clinical variants of IBS.Materials and methods:We performed a cross-sectional single center study in 79 Caucasian patients with IBS (according to the Rome criteria IV). The patients were divided into two groups: group 1, IBS with diarrhea (IBS-D, n = 45) and group 2, IBS with constipation (IBS-C, n = 34). The control group included 59 Caucasian patients with gastrointestinal disorders without IBS. Polymorphism5-HTTLPRof theSLC6A4gene was assessed in all subjects. In group 1 patients, blood serotonin levels were measured and psychological tests were performed, including Spielberger's State / Trait Anxiety Inventory, quality of life by SF36 and GSRS, Asthenia scale, VAS scores for pain intensity.Results:Thirty-five of 45 (77.8%) patients with IBS-D carried the mutantSallele, which was significantly more frequent than in the IBS-C group (p = 0.002) and in the control group (p = 0.005). There were no statistically significant differences (p = 0.54) in the frequency of detection of the homozygousLLgenotype (normal allele) and the heteroand homozygous mutant alleles (SLandSS) genotype between the IBS-C and control patients. In the IBS-D group, a gender difference for the mutantSSallele of5-HTTLPRwas found, with significantly higher frequency in female patients (p = 0.0147). No significant gender differences in the genotype distribution between the patients with IBS-C and the control group were found. There were also no differences in blood serotonin levels in the IBS patients with various5-HTTLPRtypes (p = 0.086); they were all in the reference range. However, there was a trend towards lower serotonin levels in theLLgenotype carriers compared to those with theSS/SLpolymorphisms. The Gastroenterological inventoryGSRSdemonstrated significantly higher total score for the constipation syndrome in the patients with homozygousLL 5-HTTLPRpolymorphism, compared to that in the patients with theSS/SLgenotype (p = 0.013).Conclusion:The results may be related to lower expression of theSLC6A4gene in the carriers of the mutant allele in the5-HTTLPRpromoter and subsequent decreased rate of serotonin uptake, with resulting stimulation of the gastrointestinal tract. TheSERTpolymorphism of theSLC6A4gene is worth further investigation as a potential candidate gene in the IBS pathophysiology.

Identifying blood-based biomarkers associated with aggression in weaned pigs after mixing

Animal behavior, including agonistic behavior, is regulated by various neurotransmitters and hormones in the blood. To Identify blood-based biomarkers associated with aggression in weaned pigs after mixing, a total of 500 weaned pigs from 65 litters were selected and mixed into 51 pens with littermates separately placed into different pens, and their behavior was recorded for 36 h. Blood samples were taken at 24 h before mixing and 24 h after mixing. An aggressive behavior index composite aggressive score was used to assess the aggressive behavior of weaned pigs after mixing. The most aggressive (n = 51) and the least aggressive pigs (n = 51) were selected in each pen. The concentrations of plasma triiodothyronine (T3) in the most aggressive pigs were lower (P < 0.01) than those of the least aggressive pigs at 24 h before (3.42 vs. 5.56 ng/mL) and 24 h after regrouping (3.88 vs. 5.51 ng/mL). In addition, skin lesion scores on the front body in the most aggressive pigs was greater (Pearson Chi-Square = 14.053; P = 0.001) than those of the least aggressive pigs 2 h after mixing. The concentrations of salivary cortisol from pen at 2, 24, and 48 h after mixing were higher than that at 24 h before mixing (2.2, 1.8, 2.1 vs. 0.9 ng/mL; P < 0.05). Plasma triiodothyronine levels at −24 (3.42 vs. 5.56 ng/mL; P < 0.05) and 24 h (3.88 vs. 5.51 ng/mL; P < 0.05) of mixing were higher in the most aggressive pigs than in the least aggressive pigs. The most aggressive pigs had less whole blood 5-hydroxytryptamine (293 vs. 330 ug/mL; P < 0.05) and more platelet number (1926 vs. 1571 ×109; P < 0.05) than the least aggressive pigs at 24 h after mixing. Number of defeat was positively related (r = 0.241, P < 0.05) to plasma tryptophan at 24 h after mixing. A significant canonical correlation (r = 0.7734, P = 0.006) was found between blood-based biomarkers and aggressive behavior indicators at 24 h before mixing. In conclusion, in this research blood-based biomarkers were partly associated with social stress-induced aggression in pigs, but the relationships are complicated.

SEROTONIN LEVEL IN PERIPHERAL BLOOD AND THE BRAIN’S BIOELECTRICAL ACTIVITY IN YOUNG PEOPLE 15-17 YEARS

The purpose of the work is to evaluate the features of the organization of the bioelectrical activity of the brain with different levels of serotonin in the serum of peripheral blood in young people 15-17 years old. The study involved 93 healthy girls and boys (15-17 years) of the Arkhangelsk region and the Nenets autonomous okrug. A serotonin level is determined in serum by enzyme immunoassay using a set of “Serotonin ELISA”. The electroencephalogram (EEG) power spectrum (PS) in the alpha, beta and theta frequencies ranges was recorded using an electroencephalograph “Encephalan” (Medicom, Taganrog). Age-dependent electroencephalogram (EEG) patterns is associated with the level of serotonin in peripheral blood in adolescents. On the background of a higher level of serotonin in the blood, compared to girls, boys have localized associations of theta and beta1 activity of EEG and serotonin levels, mainly in the right frontal-temporal region. In girls, the spectral power level of the EEG theta activity is more dependent on the level of serotonin in the blood, and a greater number of brain areas are involved in correlation interactions in comparison with young men (temporal regions on the left and frontal, central, parietal regions of both hemispheres of the brain).

Correlation Between Salivary, Platelet and Central Serotonin Levels in Children.

Serotonin (5-HT) is a neurotransmitter synthesized in both the central nervous system (CNS) and in enterochromaffin cells of the gut. 5-HT biosynthesis is separate between the periphery and the CNS. Any observed correlations between centrally and peripherally measured 5-HT remain to be elucidated. Previous efforts have looked for a noninvasive marker of central serotonin, including serotonin in whole blood, plasma, platelets, saliva, and urine; however, results are conflicting. Finding a noninvasive marker for central serotonin turnover that can be used for diagnosis and therapeutic monitoring in patients with primary neurotransmitter deficiencies. Inclusion criterion was all children presenting with neurological symptoms whose clinical investigations included lumbar puncture (LP) for cerebrospinal fluid (CSF) collection and neurotransmitter metabolite analysis, were recruited. For central serotonin turnover, the serotonin metabolite 5-hydroxyindoleacetic acid (5HIAA) was used. Bivariate correlation between the serotonin levels in CSF (5HIAA), platelets, and saliva was calculated. Twenty-six patients (aged 6 months to 15 years) with various neurologic presentations had LP for CSF collection and neurotransmitter metabolite analysis as part of their clinical care. An additional salivary and blood sample was obtained at the same time. Eighteen patients had suitable samples for quantitative measure of serotonin. There was no correlation between platelet serotonin and CSF 5HIAA levels (Pearson's coefficient of correlation - PCC: 0.010) or between salivary serotonin and CSF 5HIAA (PCC: 0.258). There was a strong negative correlation between salivary and platelet serotonin (PCC: -0.679). Our findings suggest that salivary serotonin measurement is not a suitable noninvasive marker for measuring central serotonin turnover.

Ginkgo biloba Extract Reduces Hippocampus Inflammatory Responses, Improves Cardiac Functions And Depressive Behaviors In A Heart Failure Mouse Model.

BackgroundDepression has been shown to share an extremely high comorbidity with heart failure (HF). Ginkgo biloba extract (GBE) is a widely used traditional Chinese medicine in cardiac disease. However, its potential therapeutic effect on depressive symptoms following HF largely remains unknown. In this article, we aimed to investigate its effects in reducing depressive behaviors of a HF mouse model. Moreover, we also discussed whether its effects are associated with changes in neural inflammation and 5-hydroxytryptamine (5-HT) signaling.MethodsMice were randomly divided into three groups: sham, HF+saline and HF+GBE (150 mg/kg/d) (n=10 per group). Systolic heart failure was induced by ligating the left anterior descending coronary artery. Cardiac functions together with depressive-like behaviors were measured after 4 weeks’ treatment. Levels of brain natriuretic peptide (BNP), 5-HT, 5-HT receptor 2A (5-HT2AR), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), vascular endothelial growth factor (VEGF), hypoxia inducible factor-1 (HIF-1), (cleaved) caspase-3, Bax and Bcl-2 were analyzed by Western blot, Elisa and immunohistochemistry at the end of the experiments.ResultsGBE benefited antidepressant-like behaviors and improved cardiac functions in mice with heart failure. Levels of TNF-α, IL-1β and 5-HT were reduced in the hippocampus after the administration of GBE. Further experiments revealed that GBE also blocked the release of serotonin in the peripheral blood and triggered HIF-1 induced anti-apoptotic pathways.ConclusionGBE has potential therapeutic effects in relieving depressive status of patients with HF.

Clinic pathogenetic characteristics of computer addiction

Objective: sto study the clinical picture, psychopathological dynamics and significant pathogenetic factors of computer addiction development (CA). Materials and methods : the study included 93 patients with CA. The control group (CG) was represented by 50 healthy volunteers. Methods: clinical, psychological, paraclinical (brain MRI; EEG; daily excretion level of adrenaline, norepinephrine, dopamine; blood serotonin level; molecular genetic studies), statistical. Results: It is established, that the clinical picture of CA is represented by episodes of computer activity (CAct) and non-episode periods. Their features form a single syndrome of addiction to CAct. Increase of symptoms’ severity and their complication occur due to addition of the altered reactivity syndrome. The clinical-dynamic model of CA development is characterized by phasing with the presence of the initial stage and the stage of the expanded clinical picture. The disorder has a predisposition. The personality component of the predisposition includes the predominance of accentuations of unstable and schizoid types, low rates of internality in both groups. The morphofunctional component includes Val158Met polymorphism features of the COMT gene as predominance (59.1 %) of homozygotes for the Val allele and a high frequency of CNS structural anomalies (55 patients; 62.5 %). The gender component characterizes the predominance of males in CA. Some pathogenetic mechanisms of CA are established: impaired catecholamine and serotonin neuromediation, impaired bioelectrical brain activity with a high prevalence (47.0 %) of paroxysmal activity. Conclusions: Based on the identified pathogenetic mechanisms, the following approaches to the CA treatment are recommended: psychotherapy, psychopharmacotherapy, and their combination.

Blood Cortisol Level and Blood Serotonin Level in Depression Mice with Basil Leaf Essential Oil Treatment.

BACKGROUND:Depression is a serious public health problem. The impact of depression is enormous, ranging from decreasing work productivity, interpersonal disorders, sleep and eating disorders, susceptible to disease to an increase in suicides. In a state of depression, there was an increase in cortisol and changes in the neurotransmitter of the brain monoamine, norepinephrine and dopamine and specifically serotonin level. Treatment of depression using synthetic drugs such as the Selective Serotonin Reuptake Inhibitor (SSRI) drug which is said to be safe turns out to still have side effects, such as stomach disorders, erectile disorders, weight gain and sometimes sleep disorders. So, the usage of traditional medicines can be an alternative. One of the traditional medicines that have been studied in Indonesia was the essential oil of basil leaves, which is known to function as an antidepressant at a dose of 2.5 x 10-2 mL/kg b.w.AIM:To determine the comparison of cortisol and serotonin level between depressed mice which get basil leaf essential oil as intervention, depression mice, and normal mice.METHODS:This research was an experimental type purely using experimental animal models, with an experimental research design Post Test Only Context Group Design in mice (Mus musculus), by dividing 3 groups of mice, namely depressed mice which received essential oil of basil leaves dose of 2.5 x 10-2 mL/kg BW, depression mice, and normal mice, to compare the level of blood cortisol and serotonin while to determine effect of administration basil leaves essential oils in depression mice.RESULTS:Cortisol levels between intervened depression mice, depression mice, and normal mice groups were significantly comparable with p < 0.001, while serotonin level was significantly comparable between intervened depression mice, depression mice, and normal mice group with p < 0.001.CONCLUSION:Evidenced by differences in cortisol levels, serotonin levels between normal mice groups, depressed mice and mice that received essential oils of basil leaves. The comparisons showed that the cortisol level of the intervened depression group was lower than the depression mice group; however the level didn’t reach the level of cortisol in the normal group. While the serotonin level of intervened depression mice group was higher than the depression mice group or normal mice group.

Investigating Potential Biomarkers in Autism Spectrum Disorder.

BackgroundEarly identification and treatment of individuals with autism spectrum disorder (ASD) improves outcomes, but specific evidence needed to individualize treatment recommendations is lacking. Biomarkers that could be routinely measured within the clinical setting could potentially transform clinical care for patients with ASD. This demonstration project employed collection of biomarker data during regular autism specialty clinical visits and explored the relationship of biomarkers with clinical ASD symptoms.MethodsEighty-three children with ASD, aged 5–10 years, completed a multi-site feasibility study integrating the collection of biochemical (blood serotonin, urine melatonin sulfate excretion) and clinical (head circumference, dysmorphology exam, digit ratio, cognitive and behavioral function) biomarkers during routine ASD clinic visits. Parents completed a demographic survey and the Aberrant Behavior Checklist-Community. Cognitive function was determined by record review. Data analysis utilized Wilcoxon two-sample tests and Spearman correlations.ResultsParticipants were 82% male, 63% White, 19% Hispanic, with a broad range of functioning. Group means indicated hyperserotonemia. In a single regression analysis adjusting for race and median household income, higher income was associated with higher levels of blood serotonin and urine melatonin sulfate excretion levels (p = 0.004 and p = 0.04, respectively). Melatonin correlated negatively with age (p = 0.048) and reported neurologic problems (p = 0.02). Dysmorphic status correlated with higher reported stereotyped behavior (p = 0.02) and inappropriate speech (p = 0.04).ConclusionThis demonstration project employed collection of multiple biomarkers, allowed for examination of associations between biochemical and clinical measures, and identified several findings that suggest direction for future studies. This clinical research model has promise for integrative biomarker research in individuals with complex, heterogeneous neurodevelopmental disorders such as ASD.

Stereological Investigation of the Rat Ventral Thalamic Nuclei Following Developmental Hyperserotonemia

Elevated blood serotonin in perinatal development is the most consistent neurochemical finding reported in Autism Spectrum Disorder (ASD), and has been implicated in the pathogenesis of the disorder. Accordingly, pre- and postnatal administration of the non-selective serotonin agonist, 5-methoxytryptamine (5-MT), is hypothesized as a model of developmental hyperserotonemia (DHS) to investigate the behavioral and morphological implications in ASD. Our previous study, examining the effects of DHS, found significant neuroanatomical changes in the dendritic architecture and connectivity of neurons in the dentate nucleus of the cerebellum. The present investigation has gone further to describe alterations in the development of the dentate-thalamo-cortical pathway, a neural network involved in motor learning, automaticity of movements, and higher cognitive functions affected in ASD. Using unbiased stereological techniques, serial sections of DHS rats were compared to age-matched controls. Analysis was performed on nuclear volume, estimated cell number, area, distribution, and volume within the principle nuclei of the thalamus. While results did not show a change in the overall volume of the thalamus, when grouped by estimated total brain volume, the mean thalamic volume was significantly reduced in the DHS group relative to controls. Additionally, significant reductions in cell numbers, density and distribution were observed in subdivisions of the principle nuclei including the ventral anterior, ventral lateral, ventral posterolateral, and ventral posteromedial nuclei. Alterations in these areas and their reciprocal connections throughout the brain may effect neuronal organization and be implicated in the neuropathological and behavioral changes observed in ASD.

Effect Of Electromagnetic Treated Water Combined With Infrared Radiation On Post-exercise Recovery Of Elite Gymnasts

Gymnasts are required to complete a series of highly difficult routines that need excellent physical condition and plenty of training time, the post-exercise recovery is crucial. Electromagnetic Treated Water (ETW) is very small water molecule clusters caused by the electromagnetic field which can benefit human body in different ways. Infrared radiation (IR) is commonly used in the recovery period of training. PURPOSE: The aim of the study is to assess the effect of ETW combined with IR on post-exercise recovery. METHODS: Twenty gymnasts from China men's national gymnastics team were randomized to the experimental group (EG, N=10) or the control group (CG, N=10). The CG continued with their daily training, the EG received 3-dimensional IR in a specific cabin for 30 minutes after training and drank no less than 1500 mL ETW per day for 12 weeks. Both groups followed the same training plan, diet and nutritional supplements. Blood parameters, including Routine Blood Test (RBT), Creatine Kinase(CK), Blood Urea Nitrogen(BUN), Dopamine(DA), Serotonin(5-HT) and Blood Lactate Acid(BLA), were detected before(t1), after(t2) and 1 hour later(t3) of training at D1, D42 and D84. In addition, Athlete Burnout Questionnaire (ABQ) and Pittsburgh Sleep Quality Index (PSQI) were collected. RESULTS: Comparing with CG, BLA(t3) and 5-HT(t3) was significantly decreased at D1(0.73±0.26 vs. 1.21±0.34 mmol/L, 160.36±26.96 vs. 189.27±40.24 ng/mL, p<0.01, respectively), BUN(t1) was lower at D84 (4.22±0.98 vs. 5.88±1.07mmol/L, p<0.01), as were Physical/Emotional Exhaustion( 9.4±2.80 vs. 13.0±2.49, P<0.01) and Sport Devaluation (9.3±2.75 vs. 12.1±2.02, p<0.05). PSQI was significantly improved at D42 (5.0±1.33 vs. 6.9±2.38, p<0.05) and D84 (3.5±1.65 vs. 6.6±2.55, p<0.01). BLA(t3) were significantly decreased compared with BLA(t2) in EG at D1, D42, D84 (0.73±0.26 vs. 2.50±0.83, 2.40±1.26 vs. 8.21±5.09, 2.05±0.63 vs. 3.46±1.33 mmol/L, p<0.01, respectively). CONCLUSION: The result of the present prospective study confirmed that ETW combined with IR can eliminate the BLA efficiently, reduce fatigue accumulation, improve sleep quality and decrease athlete burnout, that all can promote the post-exercise recovery.

Increasing serotonin bioavailability in preweaned dairy calves impacts hematology, growth, and behavior

Peripheral serotonin has been shown to regulate important physiological functions such as energy homeostasis and immunity, particularly in rodent and humans, but its role is poorly understood in livestock species. Herein, we tested the safety and effectiveness of increasing serotonin bioavailability in preweaned dairy calves by oral supplementation of a serotonin precursor (5-hydroxytryptophan, 5-HTP) or a serotonin reuptake inhibitor (fluoxetine, FLX). Bull Holstein calves (21 ± 2 d old; N = 24) were fed milk replacer (8 L/d) supplemented with either saline as control (CON, 8 mL/d, n = 8), FLX (40 mg/d, approx. 0.8 mg/kg; n = 8), or 5-HTP (90 mg/d, approx. 1.8 mg/kg; n = 8) for 10 consecutive days in a complete randomized block design. Heart rate (HR), respiration rate, rectal temperature, and health scores were recorded daily. Hip height and body weight were measured at d 1, 5, and 10 relative to initiation of supplementation. Blood samples were collected once before the supplementation period (d 1), during the 10-d supplementation period (daily), and during a 14-d withdrawal period (d 2, 3, 4, 7, and 14 relative to initiation of withdrawal). Cerebrospinal fluid and muscle tissue were collected from a subset of calves (n = 12) that were euthanized after the 10-d supplementation or 14-d withdrawal period. Whole blood serotonin concentrations increased in 5-HTP calves and decreased in FLX calves compared with CON (P < 0.001), indicating that serotonin bioavailability was increased in both groups. Whole blood serotonin concentrations of 5-HTP and FLX calves returned to CON levels after 7 d of withdrawal. All calves grew and were considered healthy throughout the study. In fact, calves fed 5-HTP had higher average daily gain compared with CON (0.87 vs 0.66 ± 0.12 kg/d, P = 0.05). Calves fed FLX had lower HR (P = 0.02) and greater red blood cells and hemoglobin counts on d 10 of supplementation compared with CON (P < 0.01). After the 14-d withdrawal period, FLX was not detected in circulation of FLX calves, but was still present in the muscle tissue. Our results demonstrate that manipulation of the serotonin pathway by supplementing FLX or 5-HTP is a feasible and safe approach in preweaned dairy calves; however, it takes more than 14 d for FLX to be completely withdrawn from the body.

Clinical Study of the Serotonin, Melatonin, Estradiol, and Adiponectin Hormones in Women with Breast Cancer in Thi – Qar Governorate -Iraq

Objective: Breast cancer is the most widely cancer among women, involving 18% of all female cancers, and worldwide, breast cancer is the fifth most common cause of cancer mortality. The study was designed to determine and compare the levels of Serotonin (ST), Melatonin (MT), Estradiol (E2), and Adiponectin (ADP) Hormones in Breast cancer patients and apparently healthy individuals. Material and Methods: Blood Serotonin (ST), Melatonin (MT), Estradiol(E2), and Adiponectin (ADP) Hormones levels were determined in 85 Breast cancer patients and 55 apparently healthy subjects. Results: The levels of serum Serotonin (ST), Melatonin (MT), and Adiponectin (ADP) Hormones were showing significant decrease in Breast cancer patients as compared to control group. ( P ≤ 0.05 ).While the level of serum Estradiol (E2) was showing significant increase in Breast cancer patients as compared to control group.( P ≤ 0.05 ). Conclusion: In Breast cancer patients, we finding decrease in Serotonin (ST), Melatonin (MT), and Adiponectin (ADP) Hormones. While we finding increase in Estradiol (E2) in Breast cancer patients as compared to control group.

Role of Serotonin and Dopamine in Psoriasis: A Case-Control Study.

BACKGROUND:Psoriasis is a chronic inflammatory disease mediated by the immune system with increased proliferation of keratinocytes. The exact cause is unknown but as a multifactor, such as infection, trauma and psychological stress have been thought to play a role in its pathophysiology. Dopamine and serotonin are believed to have a strong role in stress conditions and also directly play a role in psoriasis.AIM:This study aimed to evaluate the role of dopamine, serotonin, and psychological stress in psoriasis.METHODS:This study used a case-control design involving 30 patients with psoriasis (as a case group) and 30 healthy controls in the Dermatology and Venereology Polyclinic of Sanglah General Hospital Denpasar during the period December 2016 to February 2017. All samples were taken for venous blood examination serum dopamine and serotonin and analysed using the ELISA method. Statistical analysis using an independent t-test, partial correlation, receiver operator characteristic (ROC) curve, and logistic regression model.RESULT:There were significant differences in serotonin, dopamine, and stress index levels between groups with psoriasis and non-psoriasis (102.68 ± 25.44 Vs. 154.17 ± 20.90; p < 0.001), (437.13 ± 164.83 Vs. 138.11 ± 89.51; p < 0.001), and (138.5 ± 27.80 Vs. 92.55 ± 42.97; p < 0.001). Significant negative correlation was found between serotonin level and stress index (r = -0.366; p = 0.016) and between serotonin and dopamine (r = -0.634; p < 0.001) but a positive correlation was found between dopamine and stress index (r = 0.459; p = 0.042). Serotonin and dopamine showed that it could be used as a biochemical predictive model for psoriasis (AUC > 0.7). Multivariable risk analysis model high serum dopamine was the most important risk factor for the occurrence of psoriasis (adjusted OR: 7.8; 95% CI: 3.45-15.57; p = 0.024)CONCLUSION:Serotonin and dopamine have a significant role in the pathophysiology of the occurrence of psoriasis, and psychological stress can affect psoriasis through its influence on serotonin and dopamine.

Whole Blood Serotonin Levels and Platelet 5-HT2A Binding in Autism Spectrum Disorder.

Elevated whole blood serotonin (WB5-HT) is a well-replicated biomarker in autism spectrum disorder (ASD). Decreased platelet serotonin receptor 5-HT2A binding has been reported in ASD. WB5-HT levels and platelet 5-HT2A specific binding were obtained from 110 individuals with ASD and 18 controls. Individuals with ASD had significantly higher WB5-HT levels than controls. There was no difference in the platelet 5-HT2A specific binding between groups. Multiple regression analyses revealed that platelet 5-HT2A binding significantly predicted WB5-HT in the control sample but not in the ASD sample. These results indicate that the relationship between WB5-HT and platelet 5-HT2A binding differs depending on ASD diagnosis, suggesting differences in platelet 5-HT system regulation in ASD.

Primary carcinoid tumors at atypical sites: surgery is mandatory and size determines survival\u2014an Egyptian study

BackgroundCarcinoids at atypical sites are rare and mostly asymptomatic. In the majority, they show normal levels of serotonin and its metabolites in plasma and urine, resulting in a relatively late diagnosis and hence guarded prognosis. The relatively large size and repeated local recurrences are features of carcinoids at atypical locations and may direct the attention to its possible existence.Patients and methodsA report of six cases of carcinoid tumors in the breast, abdominal paravertebral region, duodenum, ovary, and stomach is given. Blood serotonin, plasma, and urine 5-hydroxyindole acetic acid were determined for cases suspected to have carcinoid syndrome. Those presented with a mass were radiologically studied by CT or MRI, and if a biopsy was taken (or the mass removed), keratin, CEA, and chromogranin for argentaffin reaction were studied. Follow-up was for 15 months, and a second surgery was done for the two cases (1 and 5).ResultsExcept for case 4, all patients were females. The symptoms and clinical findings in the six cases were vague and did not raise the possibility of a carcinoid. Repeated local recurrence was the clue for the diagnosis. The enrolled six cases had no common features that may point or suggest the presence of neither carcinoid tumor nor syndrome. Also, there was no relation between the site and size of the tumor and the detection of the manifestations of carcinoid syndrome. Additionally, in one case (case 1), the originally non-functioning lesion turned active when recurrence happened.ConclusionCarcinoid tumors at atypical sites are low-grade malignant neoplasms with a good long-term prognosis if correctly managed. Routine aggressive resection or even debulking is the treatment of choice whenever possible, even in recurrent cases. The survival of patients depends basically on the tumor size and/or the presence of distant metastases. Treatment by somatostatin analogy is usually mandatory if carcinoid syndrome is present.

Clinical Practice Guidelines for the management of conduct disorder.

Clinical Practice Guidelines for the management of conduct disorder.

Anesthetics influence concussive head injury induced blood-brain barrier breakdown, brain edema formation, cerebral blood flow, serotonin levels, brain pathology and functional outcome.

Several lines of evidences show that anesthetics influence neurotoxicity and neuroprotection. The possibility that different anesthetic agents potentially influence the pathophysiological and functional outcome following neurotrauma was examined in a rat model of concussive head injury (CHI). The CHI was produced by an impact of 0.224N on the right parietal bone by dropping a weight of 114.6g from a 20cm height under different anesthetic agents, e.g., inhaled ether anesthesia or intraperitoneally administered ketamine, pentobarbital, equithesin or urethane anesthesia. Five hour CHI resulted in profound volume swelling and brain edema formation in both hemispheres showing disruption of the blood-brain barrier (BBB) to Evans blue and radioiodine. A marked decrease in the cortical CBF and a profound increase in plasma or brain serotonin levels were seen at this time. Neuronal damages were present in several parts of the brain. These pathological changes were most marked in CHI under ether anesthesia followed by ketamine (35mg/kg, i.p.), pentobarbital (50mg/kg, i.p.), equithesin (3mL/kg, i.p.) and urethane (1g/kg, i.p.). The functional outcome on Rota Rod performances or grid walking tests was also most adversely affected after CHI under ether anesthesia followed by pentobarbital, equithesin and ketamine. Interestingly, the plasma and brain serotonin levels strongly correlated with the development of brain edema in head injured animals in relation to different anesthetic agents used. These observations suggest that anesthetic agents are detrimental to functional and pathological outcomes in CHI probably through influencing the circulating plasma and brain serotonin levels, not reported earlier. Whether anesthetics could also affect the efficacy of different neuroprotective agents in CNS injuries is a new subject that is currently being examined in our laboratory.

Acupuncture as a Modality for Treating Anxiety Related Disorders in Canines

Canine anxiety is a common behavior disorder that may manifest as aggression or violent behaviors and often culminates in animal surrenders. While there are sc ientifically supported behavior protocols available today, behavior problems are still a major concern for many dog owners. Thus, this case study series sought to explore an additional potential treatment modality, acupuncture. Four case reports were completed; they included two client - owned dogs and two shelter dogs. All dogs presented because of anxiety and aggression. Questionnaires completed by the pet owner throughout the study, heart rates before and after treatment, and researchers’ observations were used to compare the dog’s pre - and post - acupuncture behaviors. While the client - owned dogs were reported to have decreased anxiety and aggression, the shelter dogs were reported to have very little to no improvement in anxiety or aggression. This study was limited by the small sample size, the subjective nature of the data collected, the lack of a control group and the lack of blinding. Further research is needed to determine if acupuncture has significant therapeutic potential for canine anxiety; ideally, future studies would include a larger sample size and quantifiable data such as blood serotonin measures, endogenous opioid levels, or cortisol measures. Alternatively, future studies may include a contro l group and double blinded design.