Background: The use of rudimentary tools and crude methods to recover valuable metals from e-waste at Agbogbloshie release of harmful metals such as lead, cadmium, arsenic, and mercury into the environment. These metals, even at lower doses could be toxic and may adversely impact a wide range of health outcomes including cardiovascular health to multiple organ damage in humans. Objective: This study aimed to measure and compare levels of heavy metals and rare earth elements in the blood and urine of e-waste workers at Agbogbloshie and controls (e-waste non-exposed) at Madina Zongo. Methods: Blood and urine samples collected from 100 e-waste workers and 51 controls were analyzed for heavy metals such as Arsenic (As), Lead (Pb), Cadmium and then rare earth elements namely; (Cd), Strontium (Sr), Cerium (Ce), Europium (Eu), Yttrium (Y). Simple linear regression models were used to explore relationships between occupational characteristics and metal levels. Results: The mean blood levels of Pb (92.35±63.69 μg/L), Ce (92.35±63.69 μg/L), and Sr (50.23±18.66 μg/L) were significantly higher in e-waste workers than controls. However, mean blood Cd (0.94±0.64µg/L) and urinary As (88.57±64.41ug/L) levels were elevated in controls compared to e-waste workers. Blood levels of Eu and Y were also higher in controls than e-waste workers (p<0.05). Among e-waste workers, blood Pb levels were independent of the job task performed (p>0.05). In contrast, blood levels of As and Ce were highest collectors/sorters, followed by dismantlers and then burners. Conclusion: Although higher levels of Pb were measured in e-waste workers, the fact that higher levels of Cd, As and other rare elements were measured in controls suggests sources of exposure among the population are myriad, therefore, there is the need to conduct a larger study to determine possible sources of exposure, especially among the control and similar population.