Changes In Blood Parameters Research Articles

Exploring the feasibility of a combined exercise program for patients with advanced lung or pancreatic cancer

ObjectiveThis study aims to assess the safety, feasibility, and potential benefits of a combined aerobic and resistance exercise intervention for patients diagnosed with advanced pancreatic or lung cancer. MethodsA prospective, single-arm study was conducted, enrolling patients with advanced lung or pancreatic cancer. Participants engaged in a 12-week exercise intervention comprising personalized bi-weekly aerobic and resistance training tailored to individual baseline conditions. The primary study outcomes focused on safety (absence of serious adverse events) and feasibility. Secondary outcomes included assessments of functional capacity using the "Six minutes walking test", strength measured through handgrip and leg press tests, anthropometric measures including body mass index and waist–hip ratio, quality of life (QoL), and changes in blood parameters. ResultsThe study involved twelve patients (mean age 57.66 ​± ​7.40 years), with seven having pancreatic cancer and five having lung cancer. The recruitment rate was 50%, and assessment adherence was 100%, with an 84% adherence to the exercise program and no dropouts. No exercise-related adverse events were recorded, while three non-severe, non-exercise-related adverse events were observed: treatment-related dermatitis (Grade 2), axillary lymphadenopathy (Grade 2), and migraine (Grade 1). Significant enhancements in functional capacity, emotional well-being, and social functioning within the QoL domains were observed. Anthropometric measures, specifically waist–hip ratio and body mass index, remained stable. ConclusionsThe findings suggest that a tailored 12-week exercise intervention is both feasible and safe for patients with advanced lung or pancreatic cancer. This intervention appears to enhance functional capacity, specific aspects of QoL, and contribute to maintaining body weight.

Investigating adverse effects of chronic dietary exposure to herbicide glyphosate on zootechnical characteristics and clinical, biochemical and immunological blood parameters in broiler chickens

Although the herbicide glyphosate is widely used globally and considered safe, more evidence of its adverse effects on animals and humans is accumulating. The present investigation was aimed at evaluating the impact of different glyphosate concentrations on zootechnical characteristics and clinical, biochemical and immunological blood parameters in Ross 308 broiler chickens. Four groups were employed, including untreated control and three experimental groups fed diets enriched with glyphosate at doses of 10, 20 and 100 ppm that conformed to 0.5, 1 and 5 maximum residue limits, respectively. The results showed that glyphosate is a stress factor triggering a multifaceted effect on important blood parameters (e.g., white blood cell and phagocytic counts), which was shown for the first time in the experiments involving productive meat-type poultry. It was first revealed that glyphosate-induced changes in blood parameters may be related to a negative impact on the zootechnical characteristics including the digestive tract organ development and body weight gain. The study findings suggested that exposure to glyphosate in the feedstuffs can adversely affect the physiological condition and productivity of broilers.

Assessment of the Chronic Process in the Liver under Study Conditions Using Conventional and in-Depth Laboratory Studies

The presented article describes the work carried out on white rats in an experiment on the occurrence of severe liver pathologies, as in humans. When assessing the pathological process in the liver, in-depth laboratory studies were mainly carried out in combination with traditional examination methods. Thus, in the conducted studies, a substance with hepatotoxic properties of heliotrin, when administered according to a subtoxic dose scheme, caused severe functional and morphological changes in the liver. It was found that the change in normal and deep laboratory blood parameters characterizing inflammatory processes in the liver corresponds to morphological data.

Dose-dependent stress response of esfenvalerate insecticide on common carp (Cyprinus carpio): Evaluating blood parameters and gene expression

Esfenvalerate is a pyrethroid insecticide used primarily in the agriculture sector for insect management. Esfenvalerate is effective against a wide range of harmful insects, including flies, cockroaches, locusts, and many other types of bugs. It is also known that esfenvalerate has toxic effects on aquatic organisms and poses significant environmental concerns. In this study, the aim is to subchronically examine the effects of sublethal concentrations of esfenvalerate insecticide on common carp (Cyprinus carpio) by assessing changes in blood parameters and resulting gene expression. For this purpose, common carp (Cyprinus carpio) were divided into 5 groups and exposed to 0.025, 0.05, 0.1, and 0.15 μg/L concentrations of esfenvalerate for a period of 14 days. Blood and liver tissue samples were collected from the fish that underwent weight and length measurements. The effects on gene expression levels of immune, antioxidant, and stress-related genes in the liver tissue, including SOD, GST, Cortisol receptor, HSP70, H+−ATPase, Na+/K+−ATPase, Catalase, and GpX, were evaluated, as were the hematological and serum biochemical parameters. Significant decreases were observed in the levels of hematocrit, hemoglobin, erythrocytes, triglycerides and total protein and catalase, H+−ATPase, and GpX gene expression. Glucose, cholesterol, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase (LDH) and alkaline phosphatase (ALP), SOD, Cortisol receptor, Na+/K+−ATPase gene expression levels increased. As a result, it has been revealed that esfenvalerate insecticide causes oxidative stress in carp at all dose ranges.

Dynamics of inflammatory reaction markers in patients under endourological interventions

Introduction. Urolithiasis is one of the most widespread urological diseases and infectious complications remain one of the most common adverse events after surgical interventions for urolithiasis. Currently, in routine clinical practice in most clinics in the country, for the purpose of postoperative control and detection of infectious complications is used: a clinical blood test with assessment of the dynamics of the blood leukocyte count. Unfortunately, the current edition of the clinical guidelines does not contain recommendations on what indicators of postoperative follow-up examinations the clinician should focus on in order to identify infectious and inflammatory complications. For this reason, the study of the value of other markers of the inflammatory response appears to be an actual task.Objective. To assess the relationship between the dynamics of laboratory markers of infectious-inflammatory complications and the incidence of infectious-inflammatory response after endoscopic stone removal.Materials & methods. The prospective cohort study included 217 patients diagnosed with urolithiasis and underwent surgical removal of stones by endoscopic method. Of total patient numbers, 43 patients underwent ureteroscopy (URS) with lithotripsy, 152 patients underwent percutaneous nephrolithotripsy (PNL), and 22 patients underwent flexible ureterolithotripsy with lithotripsy. An analysis of demographics, clinical parameters and postoperative complications was performed as a descriptive analysis. Patients underwent a standard list of laboratory and instrumental examination methods. The mandatory control of body temperature was carried out t.i.d. daily. Before surgical treatment, indicators of WBC, LYMP, CRP, and ESR content were recorded on the first and second days after surgical treatment.Results. Postoperative fever was recorded in 40 (18.5%) patients. During the postoperative period, an increase in CRP and ESR was observed in both the fever and the normal temperature group, exceeding the reference values on the first day. At the same time, in the fever group (in 40 patients — 18.5%), a significantly higher increase in CRP and ESR was observed (685% vs 323%) for CRP and (146% vs 80%) for ESR. According to the results obtained in our study, CRP and ESR indicators demonstrate the most clinically significant dynamics in patients with postoperative fever, increasing by more than 500% and 100%, respectively.Conclusions. Endoscopic removal of urinary stones is a safe treatment option in patients with sterile urine cultures. After endoscopic stone removal, all patients experience such reactive changes in peripheral blood parameters as: an increase in the level of leukocytes, lymphopenia, an increase in ESR and CRP levels. Indicators of CRP and ESR demonstrate the most clinically indicative dynamics.

Blocking FGF23 signaling improves the growth plate of mice with X-linked hypophosphatemia.

Fibroblast growth factor 23 (FGF23) is a phosphaturic hormone. X-linked hypophosphatemia (XLH) is the most prevalent inherited phosphate wasting disorder due to mutations in the PHEX gene, which cause elevated circulating FGF23 levels. Clinically, it is characterized by growth impairment and defective mineralization of bones and teeth. Treatment of XLH is challenging. Since 2018, neutralizing antibodies against FGF23 have dramatically improved the therapy of XLH patients, although not all patients fully respond to the treatment, and it is very costly. C-terminal fragments of FGF23 have recently emerged as blockers of intact FGF23 signaling. Here, we analyzed the effect on growth and bone of a short 26 residues long C-terminal FGF23 (cFGF23) fragment and two N-acetylated and C-amidated cFGF23 peptides using young XLH mice (Phex C733RMhda mice). Although no major changes in blood parameters were observed after 7 days of treatment with these peptides, bone length and growth plate structure improved. The modified peptides accelerated the growth rate probably by improving growth plate structure and dynamics. The processes of chondrocyte proliferation, death, hypertrophy, and the cartilaginous composition in the growth plate were partially improved in young treated XLH mice. In conclusion, these findings contribute to understand the role of FGF23 signaling in growth plate metabolism and show that this may occur despite continuous hypophosphatemia.

Physical activity and/or dietary intervention in overweight or obese breast cancer survivors: results of the InForma randomized trial.

This study aimed to test the efficacy of a 6-month intervention on weight loss in a group of overweight or obese breast cancer (BC) survivors. We promoted adherence to a healthy diet or/and to increase physical activity, making use of a step counter device. Here we present results regarding the change in anthropometric measures and blood parameters. 266 women treated for BC with a BMI ≥ 25kg/m2 were randomized to a 6-month intervention into four arms: Dietary Intervention (DI); Physical Activity Intervention (PAI); Physical Activity and Dietary Intervention (PADI); Minimal Intervention (MI). Women were offered individualized counseling by a dietitian, a physiotherapist and a psychologist. Participants were followed up for an additional 18months. 231 women completed the 6-month intervention and 167 completed the additional 18-month follow-up. Respectively, 37.5% and 36.7% of women included in the DI and PADI arm reached the objective of the trial (weight reduction > 5%). Significant weight and circumferences decrease was observed at 6-month in the four arms. Weight decrease was more pronounced in the DI (-4.7% ± 5.0%) and PADI (-3.9% ± 4.5%) arms, persisted over time (at 12 and 24months), where counseling was mainly focused on the dietic component. The intervention had an effect on the glucose level with a significant reduction in whole population (-0.9 ± 11.7 p-value 0.02) and most pronounced in the PADI arm (-2.4 ± 7.8 p-value 0.03). Lifestyle intervention mainly focused on the dietetic component and making use of a step counter improved body weight, circumferences and glucose levels. A personalized approach yields a potential clinical benefit for BC survivors.

Therapeutic impact of biopesticide neem oil on health status of walking catfish Clarias batrachus for replacing synthetic organophosphorus pesticides

Pesticides are widely used in agricultural purposes and indiscriminate use of pesticides resulted in unintentional harming of the environment. Lack of consciousness and education coupled with indifferent attitude also contributed to indiscriminate use of pesticides. Enormous amount of these toxic pesticides and other chemicals reaches in the aquatic environment by the runoff and other sources and polluted the water, air and soil for any living organisms. One of the fastest growing food producing sectors is aquaculture, supplying approximately 40% of the world’s fish food. Pesticides may be used in aquatic ecosystem to control the fish parasites, diseases, and other pests that have also adversely affected the aquatic ecosystem. Nuvan is an organophosphorus pesticide is widely used due to low persistence in environment but also affects the non-targeted organisms including fish. Synthetic chemicals generate environmental problems; researchers have been leaning towards pest control with natural or plant based substances. Protection of water quality is possible when rationalize the use of natural pesticides and promote biopesticides. Neem oil is a natural or mixtures of chemicals obtained from neem plant (Azadirachta indica) which has many significant benefits to overcome from the chemicals or synthetic pesticides. By making the health beneficial uses of neem oil accessible to the farmers in the society, the use of chemicals in agriculture can be reduced. The aim of the current study is to observe the impacts of nuvan and neem oil on the survival and health status of fishes which also affected the other animals by the food chain. Changes in blood parameters of fish are related to the response of the whole animal, which affect on survival, reproduction and growth of fish. Some alterations in cholesterol and triglycerides in serum of treated fish Clarias batrachus with 1/20 and 1/10 LC50 concentrations of nuvan and neem oil separately compared with non-treated one after 7, 15, 30 and 60 days of exposure were recorded and discussed. Also, this assessment may be essential for encourage suitable methods of application in aquatic organism production facilities to be fully explored in future.

Fulminant acne induced by simultaneous taking of isotretinoin and anabolic steroids

Acne fulminans is a rare disease characterized by the sudden appearance of painful nodules with a pronounced tendency to ulceration, accompanied by general symptoms, including fever, arthralgia, hepatomegaly and impaired blood parameters. Among its most frequent triggers are anabolic steroids and isotretinoin.
 The article describes a patient whose disease was caused by the sequential administration of anabolic steroids and systemic isotretinoin. The clinical picture of acne fulminans in the patient was represented by typical skin rashes, changes in blood parameters, arthralgia and myalgia, as well as nodular erythema. Systemic antibacterial therapy with doxycycline was ineffective. The patient was hospitalized in the Saint Petersburg State Budgetary Healthcare Institution "City Dermatovenerological Dispensary", where he was treated with prednisone and then with systemic isotretinoin. Due to the severe course of the process, the cumulative dose of isotretinoin achieved 240 mg/kg. Against the background of treatment, studies of aspartate aminotransferases, alanine aminotransferases, bilirubin, cholesterol, triglyceride indicators were regularly conducted, the values of which did not exceed the limits of reference indicators. The remission of the disease was 3 years, there are residual phenomena in the form of scars. Scar correction is carried out using a fractional CO2 laser (SmartXide Touch), no more than 3% of the skin is treated in one procedure.
 The peculiarity of the observation presented by us is the association of fulminant acne with simultaneous administration of steroid hormones and isotretinoin, the combination of fulminant acne with erythema nodosum, as well as good tolerance of high doses of isotretinoin.
 The literature review discusses data on etiopathogenesis, features of clinical manifestations, approaches to the treatment of fulminant acne. Modern approaches to the classification of the disease are also presented.

Toxic effects of the synthetic pyrethroid permethrin on the hematological parameters and antioxidant enzyme systems of the freshwater fish Cyprinus carpio L.

This study investigated changes in hematological and antioxidant parameters of carp exposed to two different doses of synthetic pyrethroid permethrin (control, vehicle, 10 ppm, and 20 ppm) for two different periods (4 days and 21 days). Hematological analyses were then performed on a veterinary Ms4 (Melet Schloesing, France) blood counter using commercially available kits (Cat. No. WD1153). Buege and Aust for MDA, Luck for CAT, McCord and Frivovich for SOD, Lawrence and Burk methods for GSH-Px were used to determine antioxidant parameters. Decreases in RBC count, Hb amount, Hct value, granulocyte ratios, and increases in total WBC and lymphocyte ratios were statistically significant in both dose groups treated with permethrin compared to the control group (p < 0.05). However, there was no statistically significant difference in monocyte ratios (p > 0.05). Overall, permethrin exposure caused an increase in MDA levels in the liver and gill tissues of carp in both dose and duration groups compared to the control group. Also, no statistically significant difference between the two dose groups in the liver tissue was observed in terms of duration (p > 0.05). Nonetheless, the increase in MDA levels in PERM10 and PERM20 dose groups in the gill tissues over 21 days was statistically significant (p < 0.05). Furthermore, permethrin exposure increased CAT, SOD, and GSH-Px enzyme activities in the gill tissue, while impacting in the opposite direction the liver tissue. Finally, regarding MDA, CAT, SOD, and GSH-Px levels, the control, and control acetone dose groups of all experimental groups were observed to be similar (p > 0.05). As a result, permethrin produced a toxic effect on Cyprinus carpio, triggering changes in blood parameters and inducing the antioxidant enzyme system.

Alterations and predictive value of blood routine parameters in patients with lupus enteritis: A retrospective study

Background Lupus enteritis (LE) is prone to incorrect and missed diagnoses. LE primarily occurs during the active stage of systemic lupus erythematosus (SLE), which often manifests with alterations in peripheral blood cell that may serve as indicators of disease activity and organ damage. This study aims to investigate the alterations and predictive value of routine blood parameters for LE diagnosis. Methods This exploratory study retrospectively analyzed the medical records of 36 patients with SLE who were admitted to Suining Central Hospital between January 2006 and April 2023. Additionally, a control group consisting of 72 SLE patients without LE, matched for sex and age, was enrolled. A comparison was made between the two groups regarding clinical characteristics and changes in routine blood parameters. Binary logistic regression and receiver operating characteristic (ROC) curve analyses were conducted to identify independent risk factors and evaluate their diagnostic performance for LE. Results The LE group exhibited significantly higher neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR), along with a lower lymphocyte count (LYM), compared to the control group ( p &lt; .05). Binary logistic regression analysis revealed that NLR independently predicted the onset of LE, with an odds ratio of 1.347 and a 95% confidence interval of 1.070–1.696 ( p &lt; .01). ROC curve analysis demonstrated that NLR had an area under the curve of 0.68 for diagnosing LE ( p &lt; .05). When the cutoff value was set at 2.44, the Youden Index was only 0.31. Conclusion Significant differences were observed in several routine parameters between patients with LE and the control group, which can be attributed to the occurrence of LE during the active stage of SLE. However, only the NLR emerged as an independent risk factor for LE,and its predicting vulue for was insufficient; no blood routine parameter has been identified as an reliable predictor for LE.

Effects of supplemented multicomponent mycotoxin detoxifying agent in laying hens fed aflatoxin B1 and T2-toxin contaminated feeds

The present study was conducted to determine the ability of multicomponent mycotoxin detoxifying agent (MMDA) in feed to prevent the gastrointestinal absorption of aflatoxin B1 (AFB1) and T2-toxin supplemented via spiked maize. For comparisons, hens were fed with uncontaminated basal diet without or with addition of MMDA at 2 g/kg feed. The trial consisted of 105 laying hens (Lohmann Brown) without obvious signs of disease allocated to 7 treatment groups in 35 pens. Responses were demonstrated on laying performance and health status throughout the 42 d experimental period. The results of laying performance indicated significantly decreased egg mass with increasing mycotoxin (AFB1 and T2-toxin) levels up to the maximum tolerated dosage, however simultaneous presence of MMDA laying performance was slightly modified linearly to increasing application. Dose-dependent pathological changes in liver and kidneys and their relative weights, changes in blood parameters and reduced eggshell weights were observed in the hens fed AFB1 and T2-toxin. The pathological changes in the hens fed with diets containing AFB1 and T2-toxin without MMDA were significantly higher as compared with the control group, but eggshell stability was not affected. The contents of AFB1, T2-toxin and their metabolites in liver and kidney tissues were significantly decreased in the hens supplemented with MMDA at 2 and 3 g/kg in feed. MMDA supplementation significantly reduced the deposition of AFB1, T2-toxin and their metabolites in liver and kidneys at the maximum tolerated dosage (2 and 3 g/kg) indicating specific binding to AFB1 and T2-toxin in the digestive tract as compared to the corresponding diets without MMDA. Exposure of AFB1 and T2-toxin indicated significantly decreased egg mass with increasing mycotoxin levels up to the maximum tolerated dosage because of the significantly reduced egg production. Therefore, in this study, MMDA could reduce negative effects of feeding AFB1 and T-2 to laying hens.

Vitamin B12 deficiency in turkish patients with Type 2 Diabetes Mellitus

Objective: Type 2 diabetes (T2DM) disease and related changes in blood parameters bring essential health risks. This study was carried out to determine the frequency of vitamin B12 deficiency in T2DM patients, the effect of B12 deficiency on blood glucose, HbA1c control and lipid parameters, and the B12 level in diabetes patients using metformin. Material and Methods: 287 patients with T2DM who were followed up in the Endocrinology department of Istanbul Training and Research Hospital were evaluated. After the patients called for control had fasted for 8 hours, blood tests were performed, and biochemical parameters were determined with standard laboratory techniques. Vitamin B12 &lt;180 mg/dl was accepted as a deficiency, and patients were divided into groups regarding vitamin B12 deficiency. The chemiluminescence method was used to determine serum Vitamin B12 levels. Data were analyzed using SPSS version 22.0 (IBM Corporation, Armonk, NY, USA). Results: A total of 287 T2DM patients were included in the study. B12 deficiency is present in 29.8% of T2DM patients. Vitamin B12 values were found to be lower in patients with T2DM using metformin (280.2 pg /ml ±151.06 pg/ml, p&lt;0.05); however, lower vitamin B12 values did not have a significant effect on blood sugar control (p=0,3), and hypovitaminosis Vitamin B12 was associated with elevated hypertriglyceridemia (p=0,03). Conclusion: According to the current study, serum vitamin B12 level was not associated with blood glucose and HbA1c; a relationship was found between vitamin B12 deficiency and higher hypertriglyceridemia. A significant proportion of T2DM patients using metformin had lower levels of vitamin B12 compared to T2DM patients not using metformin. Vitamin B12 levels should be monitored regularly in the follow-up of patients with T2DM using metformin.

Evaluation of haematological, serum biochemical and oxidative stress parameters in cattle naturally infected with lumpy skin disease virus.

Lumpy skin disease (LSD) is a vector-borne viral transboundary disease of cattle caused by the LSD virus (LSDV). Despite investigations on clinical and outbreak features of LSDV, information on disease pathogenesis and alternative changes in blood parameters are scarce. Keeping this in view, the current study was designed to determine haematological, serum biochemical, and oxidative stress parameters in naturally infected cattle with LSDV during the recent surge of outbreaks in Punjab, Pakistan. A total of 35 blood samples was collected from polymerase chain reaction-confirmed LSDV-infected cattle for assessment of all parameters. The haematological examination of blood samples showed a significant reduction (p < 0.05) in different variables of erythrogram and leucogram. On the other hand, differences between levels of various serum biochemical parameters with the significant increase in levels of alkaline phosphatase, serum alanine aminotransferase, aspartate aminotransferase, and blood urea nitrogen were observed in LSDV naturally infected cattle. Moreover, malondialdehyde levels for lipid peroxidation and nitrate concentration were markedly elevated whereas glutathione S-transferase fluorescent and serum superoxide dismutase enzymes showed a decrease in levels. The current study suggests that alternations in haematological and serum biochemical parameters following LSDV infection stimulate oxidative stress and such findings may be useful for early and rapid diagnosis and improvement in the treatment strategy of the disease.

Comparative effects of treatment with ferric carboxymaltose versus ferric derisomaltose in a murine model of iron-deficiency anemia

Intravenous (IV) iron preparations like ferric carboxymaltose (FCM) and ferric derisomaltose (FDI) are used to treat iron deficiency anemia (IDA) due to heavy uterine bleeding, inflammatory bowel disease, and chronic kidney disease. These preparations greatly differ in terms of their side-effect profiles (e.g, hypophosphatemia). However, studies that directly compare FCM and FDI are limited. The objective of this study was to compare the effects of FCM and FDI in a mouse model of IDA and investigate changes in blood parameters and mineral homeostasis. Female C57Bl/6J mice were fed iron-deficient diet for 5 weeks followed by IV bleeding (0.7% of body weight [b.w.]) for 3 consecutive days. On day 0 (last day of bleeding), baseline blood and urine were analyzed for hematocrit, CBC, P i and Ca 2+ . Mice were randomized to vehicle (saline, 2 μl/g b.w.; n=16), FCM (20 mg/kg; n=12), or FDI (20 mg/kg; n=12) treatment via retroorbital injection on day 0 and 7. On day 14, terminal blood, urine, spleen, and heart were collected. At baseline, compared with pre-bleeding, all mice had low hematocrit (22±1 vs 48±0.3%; P&lt;0.05) and microcytic hypochromic anemia. After 14 days, mice treated with FCM and FDI had higher hematocrit compared with vehicle (47±0.4 and 46±0.3 vs 25±1%; P&lt;0.05). WBC were similar on day 14 compared with day 0 for mice treated with FCM (8±1 vs 7±1 x10 9 /L; NS) or FDI (7±1 vs 7±1 x10 9 /L; NS); however, WBC were decreased in vehicle-treated mice (4±1 vs 6±1 x10 9 /L; P&lt;0.05). Thrombocytes were increased in vehicle-treated mice on day 14 compared with day 0 (1405±55 vs 795±23 x10 9 /L; P&lt;0.05); while there was no change in thrombocytes in mice treated with FDI (706±16 vs 831±29 x10 9 /L; NS), they were decreased in FCM-treated mice (583±41 vs 771±30 x10 9 /L; P&lt;0.05). Both FCM and FDI prevented splenomegaly that was seen in vehicle-treated mice (88±3 and 87±20 vs 263±40 mg; P&lt;0.05). Similarly, FCM and FDI prevented greater heart weight that was seen in vehicle-treated mice (0.43±0.01 and 0.45±0.01 vs 0.48±0.01 g/b.w. %; P&lt;0.05). After 14 days, mice treated with FCM and FDI had lower plasma P i compared with vehicle (1.9±0.1 and 2.1±0.1 vs 2.4±0.1 mmol; P&lt;0.05); although FCM-treated mice showed greater hypophosphatemia compared with FDI ( P&lt;0.05). There were no differences between groups for urinary P i . Plasma Ca 2+ was similar between groups on day 14; but compared with day 0, it was higher in vehicle (2.7±0.1 vs 2.5±0.1 mmol; P&lt;0.05) and FCM-treated mice (2.8±0.1 vs 2.5±0.1 mmol; P&lt;0.05). Urinary Ca 2+ /creatinine was higher in mice treated with vehicle compared with day 0 (0.4±0.04 vs 0.1±0.01 mmol/mmol; P&lt;0.05) but was lower with FCM (0.09±0.03 vs 0.14±0.04 mmol/mmol; P&lt;0.05) and FDI (0.08±0.01 vs 0.3±0.06 mmol/mmol; P&lt;0.05). In summary, our study shows that both iron preparations have similar effects on blood parameters, but mice were more hypophosphatemic when treated with FCM vs FDI. We speculate this may be due to variations in PTH and FGF23 levels. This work was supported by 1R01DK110621, VA Merit Award IBX004968A, NIDDK Diabetic Complications Consortium RRID:SCR_001415, DK076169 and DK115255 (all to TR). LT was supported by an AHA Postdoctoral Fellowship (828731). This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

MANAGEMENT OF CHRONIC KIDNEY DISEASE THROUGH AYURVEDA– A CASE REPORT

Chronic kidney disease (CKD) affects over 10% of the population and is a signicant public health problem. CKD can lead to end-stage renal disease (ESRD), cardiovascular disease, and premature death. The most common causes of CKD are diabetic nephropathy and hypertensive nephropathy. Diabetic nephropathy is characterized by slowly progressive albuminuria, worsening hypertension, and renal insufciency. Hypertensive nephropathy is more common in elderly people. Diabetic nephropathy can be correlated to upadrava of vata pradhana sannipathika prameha with increase in the Kleda bhava of medodhatu and kapha, and sthanasamsraya in vasthi. Asingle case of diabetic nephropathy admitted in IPD of Ayurveda College Coimbatore was selected for the study. And the case was managed by adopting deepana pachana, samana and rasayana chikitsa. The changes in blood parameters and RFT was noted and systematically analyzed after each treatment. It was found increase in Hb level, and reduction of values of serum creatinine, blood urea, and urinary excretion of albumin. Marked symptomatic improvement was found in the patient. This management may bring some new hope to the patients of diabetic nephropathy which terminates to chronic renal failure and ultimately to death.

The pathogenicity and immunogenicity of Pasteurella multocida in low and rich iron media

Pasteurella multocida has the highest prevalence among animal populations and may cause hemorrhagic septicemia in cattle and pneumonia in sheep and goats. It has known that the pathogenicity of this organism depends on the levels of iron. Present study attempts to evaluate any changes in blood parameters and visceral organs of mice by cultivation of P.multocida under iron sufficient and insufficient conditions. P.multocida was cultured on three media: BHI alone, supplemented with bipyridyl and iron. They injected into the mice by intra-peritoneal rout. At the same time inactivated antigens were prepared from all medium and mice were immunized two weeks apart and blood samples were collected until weeks 9. Visceral organs were collected from dead mice for pathological studies. In contrast to immunogenicity and antibody titers, there are significant differences in pathogenicity and blood parameters in rich and limit iron condition.

Changes in routine blood parameters of patients with generalized tonic clonic seizure: a retrospective study

To analyze the alterations in routine blood parameters in these patients. Generalized tonic clonic seizure (GTCS) is one main type of generalized epilepsy. Clinicians have recently found changes in routine blood parameters in GTCS patients. A retrospective study of 45 GTCS patients and 200 healthy controls was conducted. Blood routine tests in emergency ward and on the second day of admission were retrieved. Changes in routine blood parameters, including red blood cell (RBC) count, white blood cell (WBC) count, platelet count, lymphocyte count, neutrophil count, and neutrophil-to-lymphocyte ratio (NLR) on the seizure episode day and the second day after seizure were analyzed. The GTCS patients had increased platelet count, RBC count, WBC count, neutrophil count, lymphocyte count, and NLR, especially at onset of GTCS episode, compared to the controls. These parameters had decreased on the second day after seizure onset. The neutrophil count and WBC count in GTCS cases with seizure duration <5min were significantly lower than those with seizure duration >5 min. However, this phenomenon was not observed in other indices. The GTCS may induce a transient increase in RBC count, WBC count, and platelet count, and obvious elevation in neutrophil count. There is a certain correlation between WBC count and the duration of seizures. Changes in routine blood parameters observed in GTCS patients may provide useful information clinically.

Effects of Different Rapid Weight Loss Strategies and Percentages on Performance-Related Parameters in Combat Sports: An Updated Systematic Review.

Most combat sports (CS) are structured in weight categories, and it is very common to carry out body weight adjustment strategies in order to compete in lower weight categories. For this reason, different rapid weight loss (RWL) strategies are usually performed to pass the pre-competition weigh-in test, and then a replenishment of fluids and carbohydrate-rich foods is conducted in an attempt to recover the weight and avoid a performance loss. In this context, no clear references have been found on whether these types of strategies have negative effects, impairing the athlete's combat and/or physical performance. For this reason, the aim of this study was to review the scientific literature on the effect of rapid weight reduction strategies on the performance of CS athletes. A literature search was performed through four different databases (PubMed, SPORTDiscus, Web of Science and ScienceDirect). Four inclusion criteria were established as follows: (1) the subjects had to be competitors in the CS and carry out RWL strategies; (2) at least two measurement points, that is, normal conditions and dehydration condition; (3) measurements in a real competition or simulating the same conditions; (4) original research articles written in English or Spanish and available in full text. Finally, a total of 16 articles were finally included in this research. All subjects (n = 184) were athletes from combat disciplines, with a minimum of 3-4 years of practice, as well as with certain experience in RWL. Six of the studies reported that an RWL strategy of around 5% of body weight loss did not affect performance parameters. However, the other ten studies with RWL between 3 and 6% or even higher reported negative effects or impairments on different parameters related to performance and/or athlete's psychophysiology, such as perceived fatigue, mood states, strength and power production, as well as changes in hormonal, blood and urine parameters, body composition, or the kinematics of the technical gesture. Although there is still no clear answer to the issue approached in this research, in general terms, it seems that in order to guarantee an acceptable athletic performance of the competitor, the weight loss should not exceed 3% to ≤5% of body weight together with ≥24 h for adequate (or at least partial) recovery and rehydration processes. In addition, it is highly recommended to lose weight progressively over several weeks, especially focusing on competitions lasting several days, as well as multiple rounds or qualifying stages.

АНАЛІЗ ПОКАЗНИКІВ КРОВІ ТА КОПРОГРАМИ ПРИ ІНФЕКЦІЙНИХ ПАТОЛОГІЯХ ТРАВНОГО ТРАКТУ У СОБАК

Реакцію організму в разі будь-якого захворювання оцінюють не тільки за клінічними ознаками, але й за результатами досліджень біологічних субстратів організму. У процесі вивчення основних гематологічних показників собак, клінічно хворих на інфекційні патології травного тракту, виявлено помірну еритроцитопенію, стійку лейкоцитопенію, олігохромемію, гіпопротеїнемію, зниження кольорового показника крові, гематокритної величини та збільшення ШОЕ. Показники лейкограми свідчать про розвиток помірної нейтрофілії, чітко вираженої лімфоцитопенії, що призводить до виникнення імуносупресії, більш важкому перебігу хвороби. Копрограма собак із гострим перебігом інфекційних ентеропатій характеризувалась підвищеним вмістом білка, стеркобіліногену, білірубіну, неперетравленого крохмалю, мил і нейтрального жиру. Отже, зміни гематологічних показників і копрограми за інфекційних патологій травного тракту собак об’єктивно треба брати до уваги під час вибору терапевтичних засобів із метою ефективного їх лікування.