Introduction: An aspiration and biopsy of bone marrow is a procedure which done for patients may be have a blood disorder or a suspected or previously diagnosed blood malignancy. Bone marrow examination is done during the initial diagnosis and/or staging and used to determine treatment response in patients with leukemia, lymphoma, and other blood disorders. This study aimed to evaluate the effect of an interventional program on nurse’s knowledge & practices related to nursing management for patients with Leukemia after bone marrow aspiration and biopsy at blood diseases center in medical city directorate. Material and Method: A pre experimental study design (one group Pretest-Posttest study design) on 20 nurses who work in bone marrow examination unit, it is carried out through the application of pre-test and post-test approach for the study group, from the period 13th June to 13th of December 2021. Three sessions were submitted to a study group that participate in an interventional program. The instrument was consist from three parts, the 1st is the nurses demographical characteristics, the 2nd assess nurses knowledge about bone marrow examination, and the 3rd deals with nurses practices about bone marrow examination.