Blood Group Subjects Research Articles

ABO, Rh Blood Groups and Haemoglobin Genotype Associations with Helicobacter pylori Infection amongst Indigenes of Wiyaakara, Rivers State

Introduction: This study aims to determine the association of ABO/Rh Blood groups and haemoglobin genotype with Helicobacter pylori infection among indigenes of Wiyaakara, Ogoni, Rivers State. This is a cross-sectional study carried out in Wiyaakara, Khana Local Government Area in Rivers State to determine the prevalence of Helicobacter pylori infection and its association with ABO/Rh blood group and haemoglobin genotype.The study was carried out amongst indigenes of Wiyaakara village in Khana LGA of Rivers State, Nigeria. Wiyaakara is part of Ogoni. Materials and Methods: Determination of ABO/Rh blood group was done using tile method. Haemoglobin genotype was determined using cellulose acetate paper electrophoresis while determination of Helicobacter pylori infection was done using the rapid diagnostic test strip method. Results: Out of 130 participants, 48.5% tested positive for Helicobacter pylori infection. Amongst those that tested positive, 68% were females while 32% were males. In ABO blood grouping, blood group O recorded the highest infectivity rate, followed by blood group A, blood group B and AB. Rh positive individuals recorded high frequency of occurrence than Rh negative. Haemoglobin genotype AA recorded high frequency of occurrence than that of AS. Based on odd ratios, the risk for the studied subjects to be infected with Helicobacter pylori was in the order of B>A>O (odd ratios: A = 1.78, B = 9.01, O = 0.54), considering the fact that AB blood group subjects was negligible due to fewer number of subjects (5). Haemoglobin AS subjects were more prone to having Helicobacter pylori than AA subjects. Conclusion: The study has revealed that blood group B individual are more at risk of being infected with Helicobacter pylori in comparison to other ABO blood groups. Additionally, those with haemoglobin AS genotype were also at risk of being infected with Helicobacter pylori than those with haemoglobin AA genotype.

Study of Fingerprint Patterns in Relation to Gender and ABO Blood Groups

Fingerprints are considered as the best tool of identification of a person. Fingerprint evidence is by far the mosteffective and reliable evidence in the court of law. The reason being fingerprint pattern persist same throughoutthe life and no two individual has similar pattern. Both blood group and dermatoglyphic pattern have geneticinheritance so an attempt has been made to analyze their correlation with gender and blood group of an individual.The objective of the study was to determine correlation (if any) between the fingerprint pattern, blood group andgender. The study was conducted on 150 students (88 males and 62 females) in the age group of 18-25 years.The fingerprint pattern of the study participants was compared with the gender and ABO-Rh blood group aftertaking their Informed written consent. Amongst ABO blood group, the most common blood group was O (40%),followed by B (35.33%), A (19.33%) and AB (5.34%). Rh + ve was the most common rhesus factor (97.33%). The loopwas the most common pattern (69.14%), followed by whorl (26.06%) and arch (4.80%). Frequency of loops werehighest in both the Rh-positive and Rh- negative subjects of ABO blood groups except O -ve blood group wherewhorls dominate. Loops are maximum seen in blood group B while whorls are more common in blood groupO and arches with blood group A. The present study confirms that loop is the most common type of fingerprintpattern while arch was the least common. The study revealed significant association between the Fingerprintpattern, Gender and ABO blood group.

ANALYSIS AND CORRELATION OF FINGER PRINTS WITH BLOOD GROUPS AMONG PEOPLE OF VISAKHAPATNAM DISTRICT, ANDHRAPRADESH-INDIA

INTRODUCTION: Crime scene has a specic identity, so are the criminals. Forensic medicine smells the criminal from scene of crime. It shows the role-play of criminals in the event of crime through detecting certain evidences. Finger prints are genetically inherited and are immutable from birth to death, hence considered as the best tools of identication. Literature states that there is a correlation between the physical characters and blood groups. Identication of nger prints along with the blood groups which are most common evidences in the crime areas, would drive the forensic scientist towards more reliable, accurate and denitive investigation. AIM OF THE STUDY: A. To Determine The Incidence Of Different Patterns Of Finger Prints Among Abo Blood Groups. B. To Ascertain The Correlation Between Finger Prints And Blood Groups. MATERIALS AND METHOD: The present study was carried out among 200 people aged 20-25 years in Visakhapatnam district, Andhra Pradesh using simple random sampling technique. Blood groups of all the subjects were determined in the pathology lab of the college. The nger prints were analyzed by two authors with the help of powerful hand magnifying lens. The observations were categorized accordingly and correlated with the blood groups. RESULTS & CONCLUSION: The study disclosed higher incidence of distribution with loop pattern followed by whorl pattern in O blood group subjects. Comparing the nger prints with blood groups there was no signicant correlation though the entities had their own signicance when analyzed individually.

Influence of ABO Blood Group on Fibrinogen Levels and Platelet Count in Apparently Healthy Nigerian Subjects

A total of hundred (100) apparently healthy young adult male and female participants comprised of 25 subjects of blood group A, B, AB and O respectively were recruited for this study. Five milliliters of blood was collected and used for the estimation of fibrinogen levels, platelet count and ABO blood grouping using standard laboratory methods. Statistical package for social science (SPSS) version 20 was employed in the analysis of the data.

Rh factor and Autonomic Function Test in Normal and Deep Breathing

Background and Aim Studies have revealed a relationship between erythrocytic antigens of the ABO and Rh blood systems and cardiovascular pathology. Reduced heart rate variability (HRV) indicates an increased risk factor for the development of cardiovascular diseases. The present study was aimed to do correlative analysis of Rh blood groups and HRV in normal individuals. Materials and Method This cross sectional study includes 120 healthy medical students (18–22 years) of both sexes, from Kasturba Medical College, Mangalore, The study was approved by institutional ethical committee and study was carried out with written informed consent of students. All the subjects were grouped in to Rh positive blood group and Rh negative blood groups based on the result of standard slide haem agglutination technique. ECG of all the subjects was recorded in lead II for a period of five minutes and HRV was analysed by measuring the R-R intervals using a software “HRV soft 1.1 Version” software package. The HRV was analyzed both by the time domain method and frequency domain analysis during normal breathing. HRV is also analysed by deep breathing test for one minute and analyzed by the time domain method. In the time domain method, RMSSD-the square root of the mean squared differences of successive RR intervals (in milliseconds) SDNN-Standard deviation of RR intervals (in milliseconds), pNN50- percentage of differences between adjacent normal RR intervals exceeding 50 milliseconds were analyzed. In frequency domain method LF nu-Low frequency in normalized units, HF nuHigh frequency in normalized units, LF/HF-Ratio between low frequency and high frequency was measured. Statistical tests included in this study were ANOVA and student's unpaired ttest. P-value was taken as statistically significant at 5 percent confidence level (P<0.05). Results The time domain analysis and the frequency domain analysis of HRV among Rh positive blood group and Rh negative blood group subjects during normal breathing did not show any statistically significant changes. Further, comparison of HRV between Rh positive blood group and Rh negative blood groups during deep breathing using time domain method did not show any statistically significant results. Conclusions The present study, concludes that there were no significant change in the heart rate variability of Rh positive blood group and Rh negative blood groups subjects. Further research is needed including in a larger sample size to confirm these findings and to show the correlation of Rh groups and heart rate variability.

Fingerprint Patterns in Relation to Gender and Blood Group among Residents of Central Indian District

Introduction: Fingerprint patterns are genotypically determined and remain unchanged from birth to death. The present study was conducted to determine the fingerprint patterns in relation to gender and blood group. Materials and Method: A cross-sectional study was undertaken and data from a total of 300 participants (150 male and female each) aged 18-40 years was collected. The fingerprints and blood group of each subject were obtained using the standard technique. Results: the majority of the subjects, 118 (39.3%), in the study belonged to blood group B, followed by blood group ‘O’ 87 (29%), ‘A’ 72 (24%) and ‘AB’ 23 (7.7%). We observed that frequency of whorl was highest in all the individual blood groups of ABO blood group system, followed by loop and arches except in O blood group, where loops were more commonly distributed among the subjects of different blood groups. We also noted that no significant association between gender and fingerprint patterns. In addition, we also observed that no significant association between fingerprint patterns and ABO blood group. Conclusion: Our results suggest that fingerprints, gender and ABO blood groups can only be assessed independently to secure identify of an individual.

The relationship between platelet indices and ABO blood groups in healthy adults.

ABO blood groups have been suggested to have a high correlation with cardiovascular diseases (CVDs). It has also been postulated that platelet indices, including mean platelet volume (MPV) and platelet distribution width (PDW), are very important in the development and progression of CVDs. However, despite these common associations with CVDs, as far as we know, there are no studies investigating platelet indices in ABO blood groups. Thus, the aim of this study was to investigate whether platelet indices are associated with ABO blood groups. The study included 301 healthy volunteers (99 women and 202 men; mean age: 32.59±7.52years) whose blood groups were determined by the gel column method using agglutination techniques. Platelet indices were studied by an automated blood counter. No considerable differences in age, gender, or Rh factors were observed among ABO blood groups. MPV was detected to be considerably lower in O and A blood group subjects than in AB and B blood group subjects. Similarly, PDW was significantly lower in O and A blood group subjects than in B blood group subjects. Additionally, MPV in the O blood group subjects was significantly lower than in the non-O blood group subjects. Because MPV and PDW are used as markers of CVDs, individuals with O and A blood groups in this study may be considered to have a lower risk of CVDs than AB and B blood group subjects. However, prospective cohort studies involving a greater number of volunteers are needed to elucidate these relationships.

Blood groups as risk factor of hypertension in Arar population, Northern Saudi Arabia

Background: Hypertension is a disease that has a huge impact on the health of communities. Familial patterns of hypertension suggests genetic factor as an another important non-modifiable predisposing factor, and ABO blood group is one of such factors which needs to be investigated in more details. Objective: to evaluate the prevalence of hypertension in various ABO and Rh blood group subjects, and to explore any association between ABO and Rh blood groups with hypertension. Methods: A cross-sectional study was conducted in Arar city, Northern Saudi Arabia. It included 312 participants from the general population of Northern Saudi Arabia. Data were collected by a pre-designed online questionnaire which was distributed among the population. It was self-administered, after a brief explanation of the idea of the research. The questionnaire included the clear questions to collect the relevant data. Results: the study included 312 participants 28.8% aged 30-39 years, 26.0% aged 40year or more, 85.3% were females and 79.2% were highly educated. In the studied sample, 38.1% had O blood group, 30.1% had B blood group, 26% had A blood group and 5.8% had AB blood group. About fifth (20.5%) of studied sample had hypertension; from them 20.3% group A, 7.8% AB, 25% B and 46.9% group O. The present study found that there was no significant association between hypertension and blood groups as risk factors (P = 0.274). Among hypertensive cases 85.9% Rh+ve, 14.1% Rh-ve, there was no significant association between hypertension and Rh factor (P = 489). Conclusion: The findings of the present study indicated that in Arar city population, we could not find any evidence that particular ABO blood group was more susceptible to develop hypertension.

Association of Diabetes Mellitus with ABO Blood Groups &amp; Rh with

Studies have observed an association between ABO blood group and risk for obesity. Objective: to evaluate the prevalence of DM in various ABO and Rh blood group subjects, and to explore any association between ABO and Rh blood groups with DM. Methods: A cross-sectional study, conducted in Arar city, Northern Saudi Arabia. It included 312 participants from the general population of Northern Saudi Arabia. Data were collected by a pre-designed online questionnaire which was distributed among the population. It was self-administered, after a brief explanation of the idea of the research. The questionnaire included the clear questions to collect the relevant data. Results: the study included 532 participants, 318 (59.8%) were males and 119(22.4%) were suffering from diabetes mellitus. Surprisingly, more than half the cases were Type 1 diabetics (53.8%). The majority (38.7%) of the studied population had O blood group, 26.1% had B, and 23.5% had A, while AB were only observed in 11.8%. Around three-quarters (74.8%) were Rh positive and 23.5% were hypertensive. The results showed insignificant relations between ABO blood group with gender, DM and type of DM. There was a highly significant relation between DM and (Rh) blood group, while it showed insignificant relations between gender and type of DM with (Rh) blood group. Conclusion: The finding of the present study indicated that, in Arar city population, no evidence was found that particular ABO blood group was more susceptible to develop diabetes mellitus. While the study showed a highly significant relation between DM and (Rh) blood groups.

Lipid Profiles in Different ABO Blood Groups in Owerri Metropolis, South East Nigeria- A Preliminary Study

Objective: The study investigated the possibility of using blood groups as predictive indices for diseases associated with lipid metabolism.&#x0D; Methods: Lipid profiles were examined in 100 apparently healthy male (40) and female (60) subjects of different ABO blood groups aged between 18-30 years from Imo State University. Of these, 20 were blood group A, 30 were B blood type, 4 were AB blood type, and 46 were blood group O. Lipid profile parameters were determined according to enzymatic assay using a commercial kit from Randox Laboratories, United Kingdom and calculation using Friedewald’s equation. Monoclonal ABO blood grouping reagent by CLAS Technology, United Kingdom was used to determine the blood group. &#x0D; Results: Total Cholesterol (140.62 ± 21.66 mg/dl) and High-Density Lipoprotein (HDL) (96.20 ± 40.32 mg/dl) were highest in blood group B. Blood group A had the highest level of Triglyceride (80.84 ± 18.60 mg/dl) and Very Low-Density Lipoprotein (VLDL) (15.21 ± 6.24 mg/dl). Blood group O showed TC level of 130.60 ±34.76 mg/dl with the highest level of LDL (70.74 ± 20.15 mg/dl) and the lowest level of HDL (51.68 ± 20.50 mg/dl) compared to non- O blood types (P &lt; 0.05).&#x0D; Conclusion: The study revealed that blood group O might have a higher propensity for dyslipidemia, suggesting an increased risk for disease associated with lipid metabolism.

Novel association of soluble intercellular adhesion molecule 1 and soluble P-selectin with the ABO blood group in a Chinese population.

Recent studies have reported that the ABO gene can affect circulating expression levels of soluble intercellular adhesion molecule 1 (sICAM-1) and soluble P-selectin (sP-selectin) in Caucasians. However, several factors may affect the association, including the distribution and variations of the ABO gene, ethnic diversity and the inflammatory response status. The aim of the present study was to investigate this issue in Asian subjects of various blood groups. A total of 800 blood samples were randomly selected from healthy blood donors. The ABO blood groups were examined using standard serological tests, and ABO genotypes of group A and group AB specimens were analyzed. Plasma concentrations of sICAM-1 and sP-selectin were detected by standard enzyme-linked immunosorbent assays. In healthy Chinese individuals, blood group A was detected to be significantly associated with lower circulating expression levels of sICAM-1 and sP-selectin, compared with group O. Individuals with ≥1 A1 allele had significantly lower expression levels of sICAM-1 and sP-selectin compared with all other ABO groups. The data indicate the significant association of ABO blood group antigens with sICAM-1 and sP-selectin expression levels in a healthy Chinese population, independent of the specific variations and distributions of ABO blood groups among ethnic populations. This result provides evidence for the previously unidentified role of ABO blood group antigens in the regulation of the inflammatory adhesion process. Accordingly, it can be proposed that ABO blood groups may require consideration when soluble adhesion molecules are identified as predictors for cardiovascular disease.

Effects of moderate-intensity physical exercise on pharmacokinetics of factor VIII and von Willebrand factor in young adults with severe haemophilia A: a pilot study.

In persons with severe haemophilia A (pwshA), infused factor VIII (FVIII) half-life can vary according to such determinants as blood group, von Willebrand factor (VWF) level or age; however, FVIII pharmacokinetics (PK) has not been well studied in pwshA duringexercise. To investigate FVIII PK in pwshA performing moderate-intensity aerobic exercise. Twelve young-adult pwshA with the intron-22 inversion mutation, on relatively low-dose FVIII prophylaxis regimens, and relatively good musculoskeletal status were recruited. Abbreviated PK of FVIII activity and von Willebrand factor antigen (VWF:Ag) level were compared - during rest, and with 60-min exercise (2×15min each of moderate-intensity stationary cycling and treadmill walking). During rest and exercise visits, a baseline blood specimen was drawn, routine prophylaxis FVIII infused; then six blood specimens were taken over the following 24h. For all subjects, mean half-life of infused FVIII did not change significantly with exercise vs. at rest (577±190 vs. 614±163min; P=0.4131). VWF:Ag rose transiently by 40-50% for 6-8h with exercise (P<0.01), particularly in non-O blood group subjects. No musculoskeletal bleeds occurred during the study. Four×15min of moderate-intensity aerobic exercise increased VWF:Ag levels for 6-8 h, and showed no evidence of accelerated FVIII clearance or of musculoskeletal bleeding in these young-adult pwshA withrelatively good musculoskeletal status, on relatively low-dose FVIII prophylaxis regimens. However, O blood group impact would merit larger studies, with longer durations of similar or more vigorous exercise intensities.

Correlation of perceived stress with blood group A and O among medical students and its effect on lipid profile in a medical college of Eastern India

The theory that there is a possible correlation between blood types and personality characteristics, other diseases, neurological disorders have been prevalent for a long-time. Background/Aims: To find correlation of perceived stress with blood group A and O among medical students and its effect on lipid profile in a medical college of eastern India. Materials and Methods: This cross-sectional study was conducted in a time span of 6 months after taking approval from the institutional ethics committee and informed consent of the subjects. Stress level in the subjects was assessed according to the presumptive life event stress scale (PSLES). 465 subjects with scores above 200 were chosen for the study. Blood groups of the subjects were assessed (blood group O 185; A 95; B 150: AB 35 [total 465]). Lipid profiles of subjects with blood group O and A were analyzed following exposure to stress. The perceived stress scale (PSS) of Sheldon Cohen was used to measure perceived stress. Data were analyzed using SPSS version 16. Results: There was no significant difference in PSLES scores, body mass index, total cholesterol, triglyceride, high-density lipoprotein, very low-density lipoprotein between subjects of blood group O and A. PSS score was significantly higher in group O as compared to group A (22.4 ± 4.6 vs. 19.36 ± 4.9; P value 0.036*). Low-density lipoprotein-cholesterol (LDL-C) was significantly higher in group O (132.63 ± 19.43 vs. 115.59 ± 18.7; P value 0.004**). PSS scores were positively correlated with LDL with r value of 0.03 [P < 0.01]). Conclusions: The results of our study indicate that subjects with blood group perceived more stress as compared to subjects of blood group A and perceived stress significantly worsened LDL-C levels.

The clearance effect of bovine anti-Helicobacter pylori antibody-containing milk in O blood group Helicobacter pylori-infected patients: a randomized double-blind clinical trial.

BackgroundThe failure in standard triple therapy has recently increased to high levels in China, primarily because of insufficient patient compliance, antimicrobial resistance, and high costs. Effective prevention and eradication of Helicobacter pylori (H. pylori) by artificial passive immunization with orally administered bovine antibodies in the milk has been demonstrated in many animal studies, but the clinical studies that are available have shown no H. pylori eradication. This study was to evaluate the efficacy and safety of orally administered bovine anti-H. pylori antibodies for the clearance of H. pylori infecting O blood group subpopulations.MethodsTwo local epidemic H. pylori strains that were prevalent locally were screened and then used to immunize dairy cows. After confirmation of the presence of anti-H. pylori polyclonal antibodies in the milk by enzyme-linked immunosorbent assay, the milk was subsequently defatted and processed into sterile milk by pasteurization. This study was designed as a double-blind placebo-controlled randomized clinical trial. Our 61 H. pylori-infected O blood group subjects were assigned to two groups; 31 subjects were treated with bovine milk containing antibodies and 30 subjects with the placebo. The medication-based study was continued for 28 days. Subjects were followed up for 56 days. The effect was assessed by the C-14 urea breath test (UBT). SPSS 17.0 software for Windows was used to analyze the data.ResultsOf the 61 subjects enrolled, 58 completed the protocol. One volunteer in the antibodies group and two volunteers in the control group dropped out. Of the 30 antibody-treated subjects, 13 became UBT negative, whereas none of the 30 of the placebo-treated subjects became UBT negative after the medication. Of 13 UBT negative patients, 3 became positive again at the end of the follow-up. Both intention to treat and per-protocol analysis indicated a significant difference in the clearance rate of infected patients between the groups treated with bovine antibody-containing milk and the placebo (P = 0.001, P < 0.05) and no significant difference in adverse effects (P > 0.05 all).ConclusionsBovine antibody-based oral immunotherapy appears to be safe and has a significant clearance effect on intragastric H. pylori that infects O blood group adults.Trial registration: ChiCTR-TRC-14005212.

Comparison of heart rate variability in different ABO blood groups of young adults

Background: ABO blood groups have been associated with various disease phenotypes, particularly cardiovascular diseases. The analysis of the heart rate variability (HRV) signal is an important tool for studying the autonomic nervous system. Aims & Objectives: The present study was designed to find out the association between different A, B, O blood groups and HRV in normal individuals. Materials and Methods: This cross sectional study included 120 volunteered healthy medical students. The subjects were grouped into four groups A, B, AB and O. HRV was analyzed by using time domain and frequency domain method during normal breathing at rest for five minutes. HRV was also analyzed during deep breathing for one minute using time domain method. Results: The analysis of HRV showed that during normal breathing, there was no statistically significant difference (P>0.05) in HRV among the ABO blood groups. HRV during deep breathing showed that there was a statistically significant increase in SDNN value (P<0.05) (standard deviation of NN interval) in O blood group subjects compared to A, B and AB blood groups. Frequency domain method did not show any statistically significant difference in (LF nu) and (HF nu) among the ABO blood groups. Conclusion: This study showed a better HRV during deep breathing in O blood group individuals. The blood type O may offer some protection from cardiovascular diseases. High risk blood group individuals can adopt a healthier lifestyle as a preventive measure for the development of cardiovascular diseases in their future.

Non-O Blood Type Is the Commonest Genetic Risk Factor for VTE: Results from a Meta-Analysis of the Literature

It is well known that the ABO blood group exerts a major influence on hemostasis, as O blood group individuals have lower von Willebrand factor and factor VIII levels than non-O blood group subjects. To evaluate the possible clinical implication of the different ABO blood groups on the risk of developing venous thromboembolism (VTE), we conducted a meta-analysis of the existing literature. After an electronic search strategy using Medline and Embase and a manual review of abstract books of the International Society on Thrombosis and Haemostasis and of reference lists of all retrieved articles, we included in the systematic review 38 studies with 10,305 VTE cases. The prevalence of non-O blood group was significantly higher in VTE patients compared with controls with a resulting pooled odds ratio (OR) of 2.09 (95% confidence interval [CI], 1.83, 2.38; p < 0.00001). Similar findings were obtained when the genotypes A1O/BO/A2B (OR 1.73, 95% CI, 1.47, 2.05; p < 0.00001) and A1B/A1A1/BB (OR 1.87, 95% CI, 1.84, 2.44; p < 0.00001) were analyzed. The maximum VTE risk was observed in non-O-factor V Leiden patients (OR 7.60, 95% CI, 3.21, 17.99), while for G20210A prothrombin mutation it was not possible to perform a pooled analysis due to a paucity of published studies. Finally, the association between non-O blood group and VTE was weaker when provoked VTE cases were considered (OR 1.33, 95% CI, 1.18, 1.50), while it was substantially unchanged when unprovoked VTE cases were analyzed (OR 1.88, 95% CI, 1.42, 2.50). In conclusion, considering its prevalence, non-O blood group is a candidate to be one of the most important genetic risk factors for venous thrombosis.

The Risk of Venous Thrombosis Related to Increase in Body Mass Index Is Mediated by Factor VIII Induced APC-Resistance.

Abstract 453 Background:The reason why a high BMI predisposes to venous thrombosis is not clarified. People with overweight or obesity tend to be more immobile which may lead to clot formation through stasis. It is also possible that these subjects acquire a prothrombotic state. Factor (F) VIII can be released by adipose tissue through inflammatory cytokines, which consequently might induce APC-resistance. This APC-resistance could be aggravated in case FV Leiden is also present. In addition, presence of high levels of FVIII in non-O blood group subjects could worsen this further. Objective:To determine whether an association exists between BMI and APC-resistance, and whether the combination of both high BMI and APC-resistance increased the risk of venous thrombosis in the Leiden Trombophilia Study (LETS). Whether increasing FVIII levels induced APC-resistance was also studied. In a pooled analysis of LETS and a Norwegian case-cohort study (TROL), we verified if FV Leiden modified the risk of increasing BMI on the occurence of venous thrombosis and whether these risks were further increased by blood group non-O. Methods:Linear regression was used to determine the relation between increasing APC-resistance and BMI, increasing FVIII levels and BMI, increasing APC-resistance and FVIII levels, and between increasing APC-resistance and BMI adjusted for FVIII levels. Cut-off points needed to create tertile categories of APC-resistance were derived from the control-group of the LETS and the TROL population separately. Logistic regression was used to calculate odds ratios and their 95% confidence intervals, adjusted for age and sex. To make the TROL and LETS population more similar for the current analysis, we restricted the analysis in the TROL subjects to those who were younger than 70 and to those who had a DVT only (n=183 cases and n=696 controls). Results:APC-resistance increased linearly with increasing BMI. A same phenomenon was observed for FVIII, i.e. an increase of BMI led to higher FVIII levels. Increased APC-resistance was in its turn associated with an increase of FVIII levels. FVIII explained part of the relation between APC and BMI, as the slope of the regression line of APC-resistance on BMI levels decreased after adjustment for FVIII. To examine the effect of increasing BMI, independent of existing APC-resistance, on the risk of venous thrombosis, we restricted the analysis to subjects from the LETS in whom APC-resistance was not related to other factors such as FV Leiden or oral contraceptive use. In these subjects (n=237 cases and n=369 controls), the risk of venous thrombosis increased 1.4-fold for those with a BMI in the median tertile (odds ratio 1.4; 95% CI, 0.9-2.3) and 2.5-fold for those in the upper tertile (odds ratio 2.5; 95% CI, 1.6-3.9), as compared to subjects in the lowest tertile. Adjustment for APC-resistance or FVIII led to a slight decrease in these relative risk estimates. Non-FV Leiden-carriers with blood group O were only at risk of venous thrombosis when they had a BMI in the upper tertile compared to non-FV Leiden-carriers with blood group O and a BMI in the lowest tertile; (adjusted odds ratio 1.9). This risk was modestly increased when non FV Leiden carriers with non-O blood group were compared with this reference group, with adjusted odds ratios of 1.5, 2.4 and 3.4, respectively, within the BMI tertiles. This risk was strongly increased when FV Leiden carriers with blood group O were compared to the reference group, with adjusted odds ratios of 3.0, 8.3 and 9.7, respectively, within the BMI tertiles. Risk of FV Leiden carriers with non-O blood group showed the highest risk of venous thrombosis compared to the reference group, no longer in a dose-response way, with adjusted odds ratios of 40.6, 23.3 and 25.2, respectively, within the BMI tertiles. Conclusion:The increased risk of venous thrombosis in subjects with high BMI is mediated by FVIII induced APC-resistance. Subjects with FV Leiden and increasing BMI had a higher risk of venous thrombosis compared to non-carriers, and this risks was more than 20 fold increased in carriers of blood group non-O and FV Leiden. Future studies are needed to show if these risks can be downgraded by weight loss. Disclosures:No relevant conflicts of interest to declare.

Thyroid cancer in a pediatric patient affected by ulcerative colitis

iopsy for a suspicious upper gastrointestinal disease. In all f them serum sample were assayed for IgG antibodies to agA and ABO blood groups were determined by standard echniques. Results. 19 children (76%) were Hp positive by istopathology and urease rapid test. 15 of these 19 (79%) p positive patients were positive for CagA serology, too. At ndoscopic examination of the 19 infected children, hypermia and friability of the gastric antrum was observed in (37%) patients and antral nodularity appearance in 12 63%) children. The histologic examination of all infected atients showed an active microerosive gastritis and chronic astritis. The children CagA positive presented more intense yperemia of gastric antrum, associated to an important lymhoplasmacellular infiltrate and a degenerative and vacuolar esions. The 6 (24%) non-infected patients, also negative for agA serology, had a normal gastric finding but they preented cardial hyperemia and distal oesophageal erosions. We ound that seropositivity for Hp increased with age and the ate of Hp infection was not significantly different in boys and irls. Similarly, Hp serological status was not significantly ifferent between subjects of different ABO blood groups. Conclusion. Endoscopic finding of antral nodularity and ositive CagA serology suggest the presence of Hp infecion and are important markers of severe gastritis. Despite pidemiological evidence of increased peptic ulcer disease n ABO blood group O subjects, we found no association etween Hp infection and ABO blood groups.

Association studies between -1185A/G von Willebrand factor gene polymorphism and coronary artery disease.

High levels of von Willebrand factor (vWF) have been associated with cardiovascular disease. The A allele of the -1185A/G polymorphism in the 5'-regulatory region of the vWF gene was associated with the highest plasma vWF levels in a normal population. To examine the association between -1185A/G polymorphism and coronary artery disease (CAD), 173 Brazilian Caucasian subjects submitted to coronary angiography were studied. Of these, 57 (33%) had normal coronary arteries (control group) and 116 (67%) had CAD (patient group). Plasma vWF levels were higher in patients (145 U/dl) than in controls (130 U/dl), but the differences were significant only for O blood group subjects. Polymerase chain reaction amplification of the 864-bp vWF promoter region followed by AccII restriction digestion was used to identify the -1185A/G genotypes. The -1185A allele frequency was 43.1% in patients and 44.7% in controls. Allele and genotype frequencies were not significantly different between patients and controls. No association was observed between the -1185A/G genotypes and plasma vWF levels in patients or controls. These results suggest that -1185A/G polymorphism is not an independent risk factor for CAD.

Evaluation of a new automated ELISA test for von Willebrand factor using two monoclonal antibodies.

A new automated quantitative enzyme linked immunosorbent assay (ELISA) for the detection of human von Willebrand factor (vWF), VIDAS vWF, has been developed for use on the VIDAS analyser (bioMérieux). The two-step capture/tag test relies on two monoclonal antibodies (mAbs), the second one being labelled with alkaline phosphatase. The lower limit of detection of the assay is < 1 IU/dl, and the upper limit of detection is 120 IU/dl. There is no hook effect for concentrations up to 1100 IU/dl. Intra- and inter-assay precision ranges from 3% and 5%. Assays are performed preferentially using citrated plasma and in these conditions the 95% confidence intervals for normal values are 52-154 IU/dl and 60-200 IU/dl for O blood group and non-O blood group subjects, respectively. Using the lower limits of the normal range as the cut-off level, all patients tested with type 1 (n = 29) or 3 (n = 2) von Willebrand disease (vWD) would be accurately classified with the new ELISA. Comparing the VIDAS test with a conventional ELISA gave a good correlation and comparable results with type 1 and 3 vWD patients (n = 31; r = 0.99; y = 0.99x + 0.24), type 2A and 2B vWD patients (n = 20; r = 0.99; y = 1.05x-1.65) and normal subjects (n = 204; r = 0.94; y = 1.06x-2.6).