Unmanned aerial vehicles (UAVs) equipped with multispectral cameras offer great potential for applications in precision agriculture. A critical challenge that limits the deployment of this technology is the varying ambient illumination caused by cloud movement. Rapidly changing solar irradiance primarily affects the radiometric calibration process, resulting in reflectance distortion and heterogeneity in the final generated orthomosaic. In this study, we optimized the radiometric block adjustment (RBA) method, which corrects for changing illumination by comparing adjacent images and from incidental observations of reference panels to produce accurate and uniform reflectance orthomosaics regardless of variable illumination. The radiometric accuracy and uniformity of the generated orthomosaic could be enhanced by improving the weights of the information from the reference panels and by reducing the number of tie points between adjacent images. Furthermore, especially for crop monitoring, we proposed the RBA-Plant method, which extracts tie points solely from vegetation areas, to further improve the accuracy and homogeneity of the orthomosaic for the vegetation areas. To validate the effectiveness of the optimization techniques and the proposed RBA-Plant method, visual and quantitative assessments were conducted on a UAV-image dataset collected under fluctuating solar irradiance conditions. The results demonstrated that the optimized RBA and RBA-Plant methods outperformed the current empirical line method (ELM) and sensor-corrected approaches, showing significant improvements in both radiometric accuracy and homogeneity. Specifically, the average root mean square error (RMSE) decreased from 0.084 acquired by the ELM to 0.047, and the average coefficient of variation (CV) decreased from 24% (ELM) to 10.6%. Furthermore, the orthomosaic generated by the RBA-Plant method achieved the lowest RMSE and CV values, 0.039 and 6.8%, respectively, indicating the highest accuracy and best uniformity. In summary, although UAVs typically incorporate lighting sensors for illumination correction, this research offers different methods for improving uniformity and obtaining more accurate reflectance values from orthomosaics.
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