Blend Hydrogel Research Articles

Development of Blended Biopolymer-Based Photocatalytic Hydrogel Beads for Adsorption and Photodegradation of Dyes.

Blended biopolymer-based photocatalytic hydrogel beads were synthesized by dissolving the biopolymers in 1-ethyl-3-methylimidazolium acetate ([Emim][Ac]), adding TiO2, and reconstituting the beads with ethanol. The incorporation of modifying biopolymer significantly enhanced the adsorption capacity of the cellulose/TiO2 beads. Cellulose/carrageenan/TiO2 beads exhibited a 7.0-fold increase in adsorption capacity for methylene blue (MB). In contrast, cellulose/chitosan/TiO2 beads showed a 4.8-fold increase in adsorption capacity for methyl orange (MO) compared with cellulose/TiO2 beads. In addition, cellulose/TiO2 microbeads were prepared through the sol-gel transition of the [Emim][Ac]-in-oil emulsion to enhance photodegradation activity. These microbeads displayed a 4.6-fold higher adsorption capacity and 2.8-fold higher photodegradation activity for MB than the millimeter-sized beads. Furthermore, they exhibited superior dye removal efficiencies for various dyes such as Congo red, MO, MB, crystal violet, and rhodamine B, surpassing the performance of larger beads. To expand the industrial applicability of the microbeads, biopolymer/TiO2 magnetic microbeads were developed by incorporating Fe2O3. These magnetic microbeads outperformed millimeter-sized beads regarding the efficiency and time required for MB removal from aqueous solutions. Furthermore, the physicochemical properties of magnetic microbeads can be easily controlled by adjusting the type of biopolymer modifier, the TiO2 and magnetic particle content, and the ratio of each component based on the target molecule. Therefore, biopolymer-based photocatalytic magnetic microbeads have great potential not only in environmental fields but also in biomedical fields.

Synthesis and characterization of Guar gum based biopolymeric hydrogels as carrier materials for controlled delivery of methotrexate to treat colon cancer

Guar Gum has been evaluated for its importance in food and pharmaceutical industry. A blended biopolymeric hydrogel was prepared by solution casting technique using guar gum (GG), chitosan (CS), polyvinyl alcohol (PVA), chemically crosslinked with tetra orthosilicate (TEOS) and impregnated with methotrexate (MTX) to assess its drug carrying capacity against colon cancer (HCT-116). The surface morphology, chemical bonding, hydrophilicity and water absorbing capacity were analyzed by atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), contact angle measurements and swelling properties in variable conditions. Furthermore, degradation, drug release kinetics, hemocompatibility, and cytotoxicity of MTX-loaded hydrogel was tested. The release of MTX from GG/CS/PVA biopolymeric blend occurred in sustained manner. Results displayed that in 7 h 25 min duration 96% of the drug was released in phosphate buffer saline (PBS) at pH 7.4. These blends were non-hemolytic, and antiproliferative against HCT-116. Furthermore, the MTT assay has revealed that MTX-loaded hydrogel had prominently decreased the cell viability (with IC50 11.7 µg/ml) as compared to free MTX (with IC50 21.57 µg/ml). Hence, these results suggest that guar gum based hydrogels are potential biomaterials for colon cancer treatment.

Kinetics Modelling of Vitamin B12 Release in an Agar/κ-Carrageenan Hydrogel Blend

A phycocolloidal hydrogel patch is studied as a potential material for the transdermal delivery device for vitamin B12. The vitamin release kinetics from an agar/κ-carrageenan hydrogel blend as a function of mass ratio and vitamin loading. Concentration measurements were done using a colorimetric method, and the experimental data were fitted into the Korsmeyer-Peppas model, Peppas-Sahlin model, and Berens-Hopfenberg model. From the curve fitting, parameters such as first-order polymer relaxation constant and diffusivity constant were obtained. The results showed that for the Korsmeyer-Peppas model and the Peppas-Sahlin model, the release mechanism followed Fickian diffusion predominantly. On the other hand, the Berens-Hopfenberg model fit shows that the release mechanism predominantly follows non-Fickian diffusion and may need to be modified.

Electro-Responsive Conductive Blended Hydrogel Patch.

The proposed electro-responsive hydrogel has great benefit for transdermal drug delivery system (TDDS) applications. To improve the physical or chemical properties of hydrogels, a number of researchers have previously studied the mixing efficiencies of the blended hydrogels. However, few studies have focused on improving the electrical conductivity and drug delivery of the hydrogels. We developed a conductive blended hydrogel by mixing alginate with gelatin methacrylate (GelMA) and silver nanowire (AgNW). We demonstrated that and the tensile strength of blended hydrogels were increased by a factor of 1.8 by blending GelMA and the electrical conductivity was enhanced by a factor of 18 by the addition of AgNW. Furthermore, the GelMA-alginate-AgNW (Gel-Alg-AgNW) blended hydrogel patch enabled on-off controllable drug release, indicating 57% doxorubicin release in response to electrical stimulation (ES) application. Therefore, this electro-responsive blended hydrogel patch could be useful for smart drug delivery applications.

Facile fabrication of biocompatible injectable blended polymeric hydrogel with bioactive nanoformulation to improving cardiac tissue regeneration efficiency after myocardial infarction for nursing care potential applications

Recent years, cardiac vascular disease has arisen owing to acute myocardial infarction (MI) and heart failure leading to death worldwide. Various treatments are available for MI in modern medicine such as implantation of devices, pharmaceutical therapy, and transplantation of organs, nonetheless, it has many complications in finding an organ donor, devices for stenosis, high intrusiveness and long-time hospitalization. To overcome these problems, we have designed and developed a novel hydrogel material with a combination of Se NPs loaded poly(ethylene glycol)/tannic acid (PEG/TA) hydrogel for the treatment of acute MI repair. Herein, Se NPs were characterized by effective analytical and spectroscopic techniques. In vitro cell compatibility and anti-oxidant analyses were examined on human cardiomyocytes in different concentrations of Se NPs and appropriate Se NPs loaded hydrogel samples to demonstrate its greater suitability for in vivo cardiac applications. In vivo investigations of MI mice models injected with Se hydrogels established that LV wall thickness was conserved significantly from the value of 235.6 µm to 390 µm. In addition, the relative scar thickness (33.6%) and infarct size (17.1%) of the MI model were enormously reduced after injection of Se hydrogel when compared to the Se NPs and control (MI) sample, respectively, which confirmed that Se introduced hydrogel have greatly influenced on the restoration of the infarcted heart. Based on the investigated results of the nanoformulation samples, it could be a promising material for future generations treatment of acute myocardial infarction and cardiac repair applications.

Bioinks for Space Missions: The Influence of Long-Term Storage of Alginate-Methylcellulose-Based Bioinks on Printability as well as Cell Viability and Function.

Bioprinting is considered a key technology for future space missions and is currently being established on the International Space Station (ISS). With the aim to perform bioink production as a critical and resource-consuming preparatory step already on Earth and transport a bioink cartridge "ready to use" to the ISS, the storability of bioinks is investigated. Hydrogel blends based on alginate and methylcellulose are laden with either green microalgae of the species Chlorella vulgaris or with different human cell lines including immortilized human mesenchymal stem cells, SaOS-2 and HepG2, as well as with primary human dental pulp stem cells. The bioinks are filled into printing cartridges and stored at 4°C for up to four weeks. Printability of the bioinks is maintained after storage. Viability and function of the cells embedded in constructs bioprinted from the stored bioinks are investigated during subsequent cultivation: The microalgae survive the storage period very well and show no loss of growth and functionality, however a significant decrease is visible for human cells, varying between the different cell types. The study demonstrates that storage of bioinks is in principle possible and is a promising starting point for future research, making complex printing processes more effective and reproducible.

Optimization of sodium alginate-galactoxyloglucan blended hydrogel beads through ionotropic gelation method

Hydrogels are 3D crosslinking networks of hydrophilic biopolymers which can able to absorb and retain large amount of water. In this present study, the Sodium alginate (SA)- Galactoxyloglucan (GXG) blended hydrogel beads were prepared and optimized through two level optimization steps. Alginate and xyloglucan are the cell wall polysaccharides biopolymers obtained from the plant sources, Sargassum sp. and Tamarindus indica L. respectively. The extracted biopolymers were confirmed and characterized by UV-Spectroscopy, FT-IR, NMR and TGA analysis. Based on the hydrophilicity, non-toxicity and biocompatibility, SA-GXG hydrogel were prepared and optimized through two-level optimization steps. The optimized hydrogel bead formulation was characterized through FT-IR, TGA and SEM analysis. From the obtained result, it is found that the polymeric formulation GXG (2 % w/v)-SA (1.5 % w/v), cross-linker (CaCl2) concentration at 0.1 M and the cross-linking time at 15 Min showed significant swelling index. The optimized hydrogel beads are porous and show good swelling capacity and thermal stability. The optimized protocol of hydrogel beads may be useful in designing hydrogel beads for specific applications in agricultural, Biomedical and remediation sectors.

Hydrophobic Drug Carrier from Polycaprolactone-b-Poly(Ethylene Glycol) Star-Shaped Polymers Hydrogel Blend as Potential for Wound Healing Application.

Blending hydrogel with an amphiphilic polymer can increase the hydrophobic drug loading and entrapment efficiency of hydrogel-based formulations. In this study, a hydrogel formulation with star-shaped polycaprolactone-b-poly(ethylene glycol) (PCL-b-PEG) as the hydrophobic drug cargo is produced. The 4-arm and 6-arm star-shaped PCL are synthesized with different molecular weights (5000, 10,000, 15,000 g/mol) via ROP and MPEG as the hydrophilic segment is attached via the Steglich esterification. FTIR and 1H-NMR analysis showed the presence of all functional groups for homopolymers and copolymers. Mn for all synthesized polymers is close to the theoretical value while GPC spectra showed a monomodal peak with narrow molecular weight distribution (PDI:1.01-1.25). The thermal degradation temperature and crystalline melting point of synthesized polymers increase with the increase in molecular weight and number of arms. All formulations possess high drug loading and entrapment efficiency (>99%) and increase with increasing molecular weight, number of arms, and amount of polymer in the formulations. All formulations showed a sustained drug release pattern with no initial burst, which follows the Korsmeyer-Peppas kinetic model. The polymer hydrogel formulations showed antibacterial activity against E. coli and S. aureus. The hydrogel containing 4-arm PCL15k-PEG is chosen as the best formulation due to its high drug release, good antimicrobial activity, and morphology.

Novel Binary Blended Hydrogel Films (Chitosan-Vanillin Schiff Base/Locust Bean Gum and Fe(III), Cu(II) & Zn(II) Complexes): Synthesis, Characterization, Conductivity, and Antibacterial Activity

Novel Binary Blended Hydrogel Films (Chitosan-Vanillin Schiff Base/Locust Bean Gum and Fe(III), Cu(II) & Zn(II) Complexes): Synthesis, Characterization, Conductivity, and Antibacterial Activity

PH and Thrombin Concentration Are Decisive in Synthesizing Stiff, Stable, and Open-Porous Fibrin-Collagen Hydrogel Blends without Chemical Cross-Linker.

Fibrin-collagen hydrogel blends exhibit high potential for tissue engineering applications. However, it is still unclear whether the underlying cross-linking mechanisms are of chemical or physical nature. It is here hypothesized that chemical cross-linkers play a negligible role and that instead pH and thrombin concentration are decisive for synthetizing blends with high stiffness and hydrolytic stability. Different fibrin-collagen formulations (pure and with additional transglutaminase) are used and the blends' compaction rate, hydrolytic stability, compressive strength, and hydrogel microstructure are investigated. The effect of thrombin concentration on gel compaction is examined and the importance of pH control during synthesis observed. It is revealed that transglutaminase impairs gel stability and it is deduced that fibrin-collagen blends mainly cross-link by mechanical interactions due to physical fibril entanglement as opposed to covalent bonds from chemical cross-linking. High thrombin concentrations and basic pH during synthesis reduce gel compaction and enhance stiffness and long-term stability. Scanning electron microscopy reveals a highly interpenetrating fibrous network with unique, interconnected open-porous microstructures. Endothelial cells proliferate on the blends and form a confluent monolayer. This study reveals the underlying cross-linking mechanisms and presents enhanced fibrin-collagen blends with high stiffness, hydrolytic stability, and large, interconnected pores; findings that offer high potential for advanced tissue engineering applications.

Performance Comparison of PVA/SA Composite Hydrogels for 3D Printing of Cartilage Scaffolds with Different Compositions

Physical blend is the method always used to modify the properties of composite hydrogels for 3D printing in tissue engineering. In this paper, polyvinyl alcohol (PVA) blending with sodium alginate (SA) was applied to enhance the mechanical properties of SA. The PVA/SA blending hydrogels with different components (5:5, 4:6, 3:7, 2:8, 1:9, and 0:10) were prepared through cross-linking method, and their properties were studied from microstructure, mechanical property, hydrophilicity, and printability, so the optimal composition ratio of the composite hydrogel can be selected for 3D printing cartilage scaffold. Results show that with PVA increasing in composite hydrogel, the pore size of the composite hydrogel becomes smaller and more even. The tensile strength and toughness of PVA/SA hydrogels increased firstly and then decreased with the increase of PVA content, and the composite hydrogel of 2PVA/8AS has the best tensile strength. Moreover, the water content and printability of hydrogels decrease with the increase of PVA, which is in good agreement with the pore structures of hydrogels. Based on the above results, we believe that 8AS/2PVA blend hydrogel is the most suitable for 3D printing cartilage scaffolds.

Characterization and evaluation of Bletilla striata polysaccharide/konjac glucomannan blend hydrogel for wound healing.

Bletilla striata polysaccharide (BSP) is effective for wound healing and has important applications in health care. A series of blend hydrogels was designed with BSP and konjac glucomannan (KGM) in this study to overcome the deficient mechanical performance caused by the excessive dissolution of BSP without affecting its physiological activity. The interplay between them, as well as the effects of KGM concentration on the physical properties and microstructures of hydrogels, were also explored. It was proved that the frame of the hydrogel was primarily formed by KGM. BSP was dispersed uniformly and linked to KGM through hydrogen bonding, which effectively improved the physical properties, such as increasing the water-holding capacity, improving the swelling degree, and enhancing the mechanical properties. Blend hydrogel BK2-2 (containing 1.0% BSP and 1.0% KGM, w/v) was found to be the optimal formulation based on the thermal stability and microstructure, which was used for further research. In vitro experiments revealed the L929 cell proliferative effects of the blend hydrogel, and no difference was found with BSP sponge extract after 72 h of exposure. In vivo animal studies indicated that the BK2-2 accelerated wound healing compared with the control group; however, no difference was found with dressings only made of BSP. These results demonstrated that KGM improved the physical properties of BSP-based material without negatively affecting its physiological properties. Also, the BSP/KGM blend hydrogel had good comprehensive properties and is expected to be used as a wound healing material in the future.

A self-indicating and antibacterial gelatine-chitosan blended hydrogel enabling real-time quality control and sustained bioactive agent delivery.

Hydrogels are one type of materials that are widely exploited for bioactive agent delivery, partly owing to their high biocompatibility and low toxicity. When hydrogels are used as carriers, their performance in agent loading and sustained agent release are predominately determined by the gel structure, which can be largely affected by variations during gel preparation. Till now, effective and easy methods to enable monitoring of such variations in real time have been lacking, making quality control of the generated gel-based carrier technically challenging. To address this technical gap, in this study we take advantage of the clusteroluminogenic properties of gelatine and chitosan to generate a crosslinked blended hydrogel which not only shows intrinsic antibacterial properties and high tunability in delivery performance but also shows a self-indicating capacity to enable quality control during hydrogel preparation. Upon fitting the curves of agent release into different kinetic models, the release profiles of the agent-loaded gels have been found to follow the Higuchi model well, with the non-Fickian mechanism being the major mechanism of the release process. Along with their high efficiency in agent loading, our gels warrant further exploitation for use in bioactive agent delivery and related biomedical applications.

Thermoresponsive hydrogel for transcatheter arterial chemoembolization of hepatocellular carcinoma.

Transarterial chemoembolization (TACE) is one of the most commonly used treatments for hepatocellular carcinoma (HCC); however, the poor stability of emulsified chemotherapy drugs by iodinated oil always leads to serious systemic cytotoxicity. Herein, a composite hydrogel Epi/Etpoil@MC/XG was proposed by stably distributing ethiodized poppyseed oil (Etpoil) and epirubicin (Epi) in the blend hydrogel of methylcellulose (MC) and xanthan gum (XG). Benefiting from its adjusted thermo-responsive and injectable properties, the Epi/Etpoil@MC/XG has been successfully applied in the embolization of the feeding artery for a VX2 tumor model.

Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.

Biopolymers have received widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility. Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields. In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters. Crosslinking was achieved through electrostatic interactions between cationic chitosan, chitosan/gelatin, chitosan/methylcellulose and AMV (NH4VO3). The as-prepared hydrogel beads were yellow in colour and exhibited a high mechanical strength. They were characterized using FT-IR spectroscopy and SEM, to confirm hydrogel formation and evaluate their surface morphology. It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties. Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents. Due to their luminescence properties these hydrogel beads showed excellent selective sensing behaviour toward ascorbic acid with a LOD of 1.06 μM. The hydrogels were also assessed for their antibacterial activity, and were tested against Staphylococcus aureus, Escherichia coli, Streptococcus anginosus, and Klebsiella pneumoniae. The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).

Fabrication and characterization of hydrogel films using A. mylitta regenerated silk fibroin with Poly vinyl alcohol for biomedical uses

Hydrogels are water-absorbing three-dimensional structures or materials that contain more water content. In recent years, hydrogels have been used in many fields like tissue engineering where they can imitate several body tissues that are present in the body. Hydrogel films are fabricated by blending PVA and RSF at different ratios without affecting their natural properties. The hydrogel films containing PVA and RSF in various ratios are fabricated which can be further used for tissue engineering and wound healing purposes with novel properties. In this study, the gelation time was observed for 1-2 days by the vial inversion test. The SEM images showed different pore sizes and surface morphology for all the different ratios of hydrogel films. According to FTIR-ATR spectroscopy, both the RSF and PVA molecules interacted well with each other and RSF showed amorphous as well as crystalline silk I structures i.e. β-sheet in the spectrum whereas DSC and TGA are used to determine the thermal stability of the blended hydrogel films. The mechanical properties of these hydrogel films were controlled by RSF. All the blended hydrogel films showed minimal or no toxicity against NIH3T3 fibroblast cells through the cell viability test, making them promising materials for biomedical uses.

Alginate‐Laminin Hydrogel Supports Long‐Term Neuronal Activity in 3D Human Induced Pluripotent Stem Cell‐Derived Neuronal Networks

AbstractFor 3D neural cultures durable hydrogels are required, which persist over a long differentiation period and thus enable the maturation of neuronal networks (NN). Here, 3D models based on human induced pluripotent stem cell‐derived neural progenitor cells that are embedded in hydrogels of either pure alginate or alginate functionalized with the extracellular matrix protein laminin 111 (L111) are established. This study analyzes material characteristics such as porosity, L111 distribution and shear viscosity, cell compatibility of hydrogels by measuring viability and cytotoxicity, and neural function by monitoring cell migration, differentiation as well as NN formation and activity on multielectrode arrays. The addition of L111 increases neural migration and enhances differentiation into neurons and astrocytes as well as synaptogenesis in alginate hydrogels. NN formed in hydrogels are electrically active for up to 206 d and L111‐supplementation further increases electrical activity, network maturation, and synchronicity compared to 2D controls and NN grown in pure alginate hydrogels. L111 addition to alginate gels further accelerates recovery of electrical activity after blockage of sodium channels with tetrodotoxin. In conclusion, NN grown in alginate‐L111 hydrogel blends are promising models for future long‐term applications in disease modeling, drug or chemical evaluation.

PREPARATION AND RHEOLOGICAL CHARACTERIZATION OF CELLULOSE-CHITOSAN HYDROGEL FOR EXTRUSION 3D PRINTER

The application of three-dimensional (3D) printing in tissue engineering is becoming prominent nowadays. A big obstacle for this technology is the selection of proper ink material. Chitosan hydrogel is an established biocompatible material that can be used as tissue scaffolds, and it has the rheology necessary for processing via extrusion type 3D printer. However, chitosan still has degradation and swelling limitations. Hence, chitosan hydrogel blends were incorporated with cellulose particles and were prepared as ink material. The hydrogels were successfully synthesized via thermo-responsive sol-gel method. Fourier transform infrared spectroscopy (FT-IR) analysis showed that the gelation of hydrogels formed a semi-interpenetrating network of ionically crosslinked chitosan and cellulose particles. Rheological tests have shown that the hydrogels exhibited shear thinning property necessary for printing. However, high cellulose amounts caused clogging during printing, and the presence of water limited the structural rigidity of the printed product. Besides this, it was found that the addition of cellulose was able to increase swelling, decrease degradation rate, and decrease gelation time, but the effect is not significant for any of these three properties.

Fabrication of pH responsive hydrogel blends of chondroitin sulfate/pluronic F-127 for the controlled release of ketorolac: its characterization and acute oral toxicity study

Objective Ketorolac tromethamine (KT), selected as a model drug, is used in management of moderate to severe acute pain. It has a short half-life (∼5.5 h) and requires frequent dose administration when needed for longer period of time. In our current project, we designed pH responsive hydrogel blends of chondroitin sulfate/pluronic F-127 (CS/Pl) for the controlled release of ketorolac. Methods Hydrogel blends were fabricated using free radical polymerization reaction technique utilizing different ratios of chondroitin sulfate (CS) (polymer) and pluronic F-127 (polymer), acrylic acid (monomer), N,N′-methyl-bisacrylamide (MBA) (cross-linker), initiator ammonium persulfate (APS) and tween-80 (surfactant). The fabricated hydrogel blends were studied and evaluated for pH responsiveness, swelling, water absorbency, in vitro drug release, and morphological characteristics such as SEM, XRD, FTIR, and TGA/DSC. Acute toxicity study was performed on rabbits. Results Maximum swelling and water absorbency were shown by CS/Pl blends being significantly greater at 7.4 (basic pH) than in 1.2 (acidic pH). In vitro dissolution demonstrated pH responsive controlled KT release following zero order at higher pH (7.4) medium up to 36 h. FTIR studies confirmed the structures of our blends; SEM results showed porous framework; thermal studies revealed higher stability of hydrogels than the individual polymers; and XRD confirmed the nature of our blends. Toxicity study revealed the nontoxic nature of the hydrogel blends. Conclusion The prepared CS/Pl hydrogels demonstrated stimuli-controlled release with delivery of drug for prolonged period of time and thus can minimize dosing frequency, safe drug delivery, increased patient compliance and easiness.

Modelling of adsorption of nickel (II) by blend hydrogels (cellulose nanocrystals and corn starch) from aqueous solution using adaptive neuro‐fuzzy inference systems (ANFIS) and artificial neural networks (ANN)

AbstractThe current study looks at the effectiveness of the removal of nickel (II) from aqueous solution using an adsorption method in a laboratory‐size reactor. An artificial neural network (ANN) and an adaptive neuro‐fuzzy inference system (ANFIS) were used in this study to predict blend hydrogels adsorption potential in the removal of nickel (II) from aqueous solution. Four operational variables, including initial Ni (II) concentration (mg/L), pH, contact duration (min), and adsorbent dose (mg/L), were used as an input with removal percentage (%) as the only output; they were studied to assess their impact on the adsorption study in the ANFIS model. In contrast, 70% of the data was used for training, while 15% of the data was used in testing and validation to build the ANN model. Feedforward propagation with the Levenberg–Marquardt algorithm was employed to train the network. The use of ANN and ANFIS models for experiments was used to optimize, construct, and develop prediction models for Ni (II) adsorption using blend hydrogels. The adsorption isotherm and kinetic models were also used to describe the process. The results show that ANN and ANFIS models are promising prediction approaches that can be applied to successfully predict metal ions adsorption. According to this finding, the root mean square errors (RMSE), absolute average relative errors (AARE), average relative errors (ARE), mean squared deviation (MSE), and R2 for Ni (II) in the training dataset were 0.061, 0.078, 0.017, 0.019, and 0.986, respectively, for ANN. In the ANFIS model, the RMSE, AARE, ARE, MSE, and R2 were 0.0129, 0.0119, 0.028, 0.030, and 0.995, respectively. The adsorption process was spontaneous and well explained by the Langmuir model, and chemisorption was the primary control. The morphology, functional groups, thermal characteristics, and crystallinity of blend hydrogels were all assessed.