Blackwater River Research Articles

Draft Whole-Genome Sequence of Bacillus paramycoides LB_RP2, a Putative Polyhydroxyalkanoate-Producing Bacterium Isolated from an Amazonian Blackwater River.

ABSTRACTBacteria of the genus Bacillus have been investigated due to the ability that many species have of accumulating polyhydroxyalkanoates (PHA) via a wide variety of raw materials as their carbon source. Herein, we report the draft whole-genome sequence of the putative PHA-accumulating strain Bacillus paramycoides LB_RP2, isolated from an Amazonian river.

Marked Differences in Butterfly Assemblage Composition between Forest Types in Central Amazonia, Brazil

Amazonia comprises a mosaic of contrasting habitats, with wide environmental heterogeneity at local and regional scales. In central Amazonia, upland forest (terra firme) is the predominant forest type and seasonally flooded forests inundated by white- and black-water rivers (várzea and igapó, respectively) represent around 20% of the forested areas. In this work, we took advantage of a natural spatial arrangement of the main vegetation types in central Amazonia to investigate butterfly assemblage structure in terra firme, várzea and igapó forests at the local scale. We sampled in the low- and high-water seasons, combining active and passive sampling with traps placed in both the understory and canopy. Terra firme supported the highest number of butterfly species, whereas várzea forest provided the highest number of butterfly captures. The high species richness in terra firme may reflect that this forest type is floristically richer than várzea and igapó. Várzea is a very productive environment and may thus support a higher number of butterfly individuals than terra firme and igapó. Most butterfly species (80.2%) were unique to a single forest type and 17 can be considered forest type indicator species in this landscape. Floodplain forest environments are therefore an important complement to terra firme in terms of butterfly species richness and conservation in Amazonia.

DISTRIBUTION AND STATUS OF THE SUWANNEE COOTER, PSEUDEMYS CONCINNA SUWANNIENSIS, IN THE ALAFIA RIVER (HILLSBOROUGH COUNTY, FLORIDA, USA)

The Suwannee Cooter, Pseudemys concinna suwanniensis, is a geographically limited turtle of conservation concern that inhabits Florida rivers draining into the northeastern Gulf of Mexico. Threats impacting its conservation status include take for human consumption, predation of turtles and nests, loss or degradation of nesting and basking habitat, water quality degradation, and boat strikes. Our surveys revealed that the Alafia River, which drains into Hillsborough Bay (northeastern Tampa Bay), is likely the stronghold of the southern distribution of P. c. suwanniensis. Multiple survey methods during 2015-2020 revealed that a substantial population of Suwannee Cooters inhabits much of this blackwater river system, including the main channel and at least one of its two primary tributaries. GIS analysis showed that more than half of the shoreline within the occupied extent is currently protected by conservation lands, although additional protection of private lands and improved habitat management protocols are needed to assure the population’s conservation.

Estimating the Influence of Oyster Reef Chains on Freshwater Detention at the Estuary Scale Using Landsat-8 Imagery

Oyster reef chains grow in response to local hydrodynamics and can redirect flows, particularly when reef chains grow perpendicular to freshwater flow paths. Singularly, oyster reef chains can act as porous dams that may facilitate nearshore accumulation of fresh or low-salinity water, in turn creating intermediate salinities that support oyster growth and estuarine conditions. However, oyster-driven freshwater detention has only been confirmed by limited, point-scale observational data, and simplified models. Oyster reef-driven freshwater detention in real ecosystems at the estuary scale remains largely unexplored. In this study, we analyzed the visible bands in 30-m resolution remote sensing (RS) images recorded by the Operational Land Imager aboard Landsat-8 to characterize the freshwater detention effect of oyster reef chains across a set of hydrologic conditions. Our results support prior findings indicating that 30-m resolution RS images recorded by the Operational Land Imager aboard Landsat-8 are useful for analyzing coastal dynamics after atmospheric correction, despite having been originally designed for terrestrial studies. Statistical models of water-leaving reflectance revealed that freshwater detention by oyster reefs was evident across the estuary, with the greatest effect occurring in the region closest to shore. Additionally, statistical modeling results and spatial patterns apparent in the satellite images suggested that reef-driven freshwater detention occurred under high riverine discharge conditions, but was less evident when flow was low. Beyond offering insight on the potential role of oyster reefs as mediators of estuarine hydrology, this study presents a transferable methodological framework for exploring estuarine biophysical feedbacks in blackwater river estuaries using satellite remote sensing.

Growth genes are implicated in the evolutionary divergence of sympatric piscivorous and insectivorous rainbow trout (Oncorhynchus mykiss)

BackgroundIdentifying ecologically significant phenotypic traits and the genomic mechanisms that underly them are crucial steps in understanding traits associated with population divergence. We used genome-wide data to identify genomic regions associated with key traits that distinguish two ecomorphs of rainbow trout (Oncorhynchus mykiss)—insectivores and piscivores—that coexist for the non-breeding portion of the year in Kootenay Lake, southeastern British Columbia. “Gerrards” are large-bodied, rapidly growing piscivores with high metabolic rates that spawn north of Kootenay Lake in the Lardeau River, in contrast to the insectivorous populations that are on average smaller in body size, with lower growth and metabolic rates, mainly forage on aquatic insects, and spawn in tributaries immediately surrounding Kootenay Lake. We used pool-seq data representing ~ 60% of the genome and 80 fish per population to assess the level of genomic divergence between ecomorphs and to identify and interrogate loci that may play functional or selective roles in their divergence.ResultsGenomic divergence was high between sympatric insectivores and piscivores (F_{text{ST}} = 0.188), and in fact higher than between insectivorous populations from Kootenay Lake and the Blackwater River (F_{text{ST}} = 0.159) that are > 500 km apart. A window-based F_{text{ST}} analysis did not reveal “islands” of genomic differentiation; however, the window with highest F_{text{ST}} estimate did include a gene associated with insulin secretion. Although we explored the use of the “Local score” approach to identify genomic outlier regions, this method was ultimately not used because simulations revealed a high false discovery rate (~ 20%). Gene ontology (GO) analysis identified several growth processes as enriched in genes occurring in the ~ 200 most divergent genomic windows, indicating many loci of small effect involved in growth and growth-related metabolic processes are associated with the divergence of these ecomorphs.ConclusionOur results reveal a high degree of genomic differentiation between piscivorous and insectivorous populations and indicate that the large body piscivorous phenotype is likely not due to one or a few loci of large effect. Rather, the piscivore phenotype may be controlled by several loci of small effect, thus highlighting the power of whole-genome resequencing in identifying genomic regions underlying population-level phenotypic divergences.

Influence of Hydrological Variables on Macrophytes in a Black Water River Ecosystem

This study assessed the influence of hydrological variables on macrophytes in a Black Water River ecosystem of Enyong River in Itu and Ibiono Local Government Areas of Akwa Ibom and Cross Rivers States, Nigeria. Four vegetation plots were chosen and in each of the plots, four belt transect were laid. In each transect, macrophyte were systematically sampled in four 10 m × 10 m quadrat at regular intervals. Macrophytes were identified to species level and their frequency and density determined. Water samples were obtained in each quadrat where the macrophytes were sampled and analyzed for their physicochemical properties using standardized methods. Altogether, 10 macrophyte species were encountered. Vossia cuspidata had the highest density (100.00±8.00 st/ha) and frequency values (100 %). Sacciolepis africana had the least density of 7.10±0.45 st/ha while Ludwigia octovalvis, Persicaria senegalensis, andSacciolepis africana had the least frequency of 25 %, respectively. The pH of the water was strongly acidic (5.54±0.03), electrical conductivity was low (20.00±5.77 µs/cm), temperature (29.00±1.10 ºC), Dissolved Oxygen (DO) (9.20±0.12 mg/l) and turbidity (7.10±0.06 NTU) values were high while Biological Oxygen Demand (BOD) (2.00±0.29 mg/l) Total Dissolved Solids (TDS) (10.00±0.29 mg/l) and Total Suspended Solids (TSS) were low (5.00±1.15 mg/l). Water Nutrients followed this decreasing order; chloride (3.55±0.02 mg/l) > nitrate (2.45±0.03 mg/l) > sulphate (2.02±0.06 mg/l) > phosphate (0.08±0.01 mg/l) and sulphide (0.03±0.02 mg/l). Heavy metals also followed this descending order; Pb (0.50±0.03 mg/l) > Zn (0.07±0.02 mg/l) > Cu (0.03±0.02 mg/l). Canonical Correspondence Analysis delineated 11 hydrological variables (temperature, pH, sulphate, turbidity, phosphate, BOD, nitrate, DO, TDS, sulphide and TSS) which exerted great influence on macrophyte distribution. V. cuspidata had affinity to pH and temperature, Sacciolepis africana had affinity to turbidity and BOD, Ludwigia octovalvis and Nymphaea lotus had affinity to sulphate and chloride, respectively, while Ipomoea aquatica and Alternanthera sessils had affinity to phosphate. On the other hand, Persicaria senegalensis, Salvinia molesta, Azolla pinnata and Ceratophyllum demersum had affinities to sulphide, DO, TSS and TDS, respectively. Since hydrological variables regulate macrophyte diversity and distribution, this study calls for consistent, monitoring and management of this ecosystem against future environmental changes. 
 Keywords: Aquatic plants, Black water, Canonical Correspondence Analysis, Ordination

Onset of runaway fragmentation of salt marshes

Summary Salt marshes are valuable but vulnerable coastal ecosystems that adapt to relative sea level rise (RSLR) by accumulating organic matter and inorganic sediment. The natural limit of these processes defines a threshold rate of RSLR beyond which marshes drown, resulting in ponding and conversion to open waters. We develop a simplified formulation for sediment transport across marshes to show that pond formation leads to runaway marsh fragmentation, a process characterized by a self-similar hierarchy of pond sizes with power-law distributions. We find the threshold for marsh fragmentation scales primarily with tidal range and that sediment supply is only relevant where tides are sufficient to transport sediment to the marsh interior. Thus the RSLR threshold is controlled by organic accretion in microtidal marshes regardless of the suspended sediment concentration at the marsh edge. This explains the observed fragmentation of microtidal marshes and suggests a tipping point for widespread marsh loss.

The effects of dissolved organic carbon on the reflex ventilatory responses of the neotropical teleost (Colossoma macropomum) to hypoxia or hypercapnia

The tambaqui (Colossoma macropomum), migrates annually between whitewater and blackwater rivers of the Amazon. Unlike the whitewater, blackwater is characterized by higher levels of dissolved organic carbon (DOC), including humic acids (HA). Because humic substances impair sensory processes, the current study tested the hypothesis that O2 and/or CO2 chemoreception is impeded in blackwater owing to the presence of HA. Thus, the ventilatory responses of tambaqui to hypoxia or hypercapnia were assessed in well water transported from Manaus, local blackwater, and in well water containing HA either extracted from Rio Negro water or obtained commercially (Sigma Aldrich; SA). In well water, tambaqui exhibited typical hyperventilatory responses to hypoxia or hypercapnia. These responses were prevented by simultaneously exposing fish to SA HA (20 mg l−1). The negative effects of SA HA on ventilation were prevented when natural DOC (30 mg l−1; extracted from Rio Negro water after first removing the endogenous HA fraction) was added concurrently, indicating a protective effect of this non-humic acid DOC fraction. The hyperventilatory responses were unaffected during acute exposure or after acclimation of fish to Rio Negro water. HA extracted from Rio Negro water did not impair the hyperventilatory responses to hypoxia or hypercapnia. This study, while demonstrating a negative effect of SA HA derived from peat (coal) on the control of breathing in tambaqui, failed to reveal any detrimental consequences of HA (derived from the decomposition of a variety of lignin-rich plants) naturally occurring in the blackwaters of the Rio Negro.

Predicting the potential distribution of aquatic herbaceous plants in oligotrophic Central Amazonian wetland ecosystems

Aquatic herbaceous plants are especially suitable for mapping environmental variability in wetlands, as they respond quickly to environmental gradients and are good indicators of habitat preference. We describe the composition of herbaceous species in two oligotrophic wetland ecosystems, floodplains along black-water rivers (igapó) and wetlands upon hydromorphic sand soils (campinarana) in the Parque Nacional do Jaú and the Reserva de Desenvolvimento Sustentável Uatumã in Central Amazonia, both protected areas. We tested for the potential distribution range (PDR) of the most frequent species of these ecosystems, which are the ones that occurred in at least two of the sampled wetlands, using species distribution models (SDMs). In total, 98 aquatic herbaceous species were recorded, of which 63 occurred in igapós and 44 in campinaranas. Most igapó species had ample PDRs across the Neotropics, while most campinarana species were restricted to the Amazon Basin. These results are congruent with studies that described similar distribution patterns for tree and bird species, which emphasizes a high degree of endemism in Amazonian campinaranas. However, we also found differences in the potential distribution of species between the two protected areas, indicating high environmental variability of oligotrophic ecosystems that deserve further investigation to develop effective measures for their conservation and protection.

Spatiotemporal Dynamics of Suspended Sediments in the Negro River, Amazon Basin, from In Situ and Sentinel-2 Remote Sensing Data

Monitoring suspended sediments through remote sensing data in black-water rivers is a challenge. Herein, remote sensing reflectance (Rrs) from in situ measurements and Sentinel-2 Multi-Spectral Instrument (MSI) images were used to estimate the suspended sediment concentration (SSC) in the largest black-water river of the Amazon basin. The Negro River exhibits extremely low Rrs values (<0.005 sr−1 at visible and near-infrared bands) due to the elevated absorption of coloured dissolved organic matter (aCDOM at 440 nm > 7 m−1) caused by the high amount of dissolved organic carbon (DOC > 7 mg L−1) and low SSC (<5 mg L−1). Interannual variability of Rrs is primarily controlled by the input of suspended sediments from the Branco River, which is a clear water river that governs the changes in the apparent optical properties of the Negro River, even at distances that are greater than 90 km from its mouth. Better results were obtained using the Sentinel-2 MSI Red band (Band 4 at 665 nm) in order to estimate the SSC, with an R2 value greater than 0.85 and an error less than 20% in the adjusted models. The magnitudes of water reflectance in the Sentinel-2 MSI Red band were consistent with in situ Rrs measurements, indicating the large spatial variability of the lower SSC values (0 to 15 mg L−1) in a complex anabranching reach of the Negro River. The in situ and satellite data analysed in this study indicates sedimentation processes in the lower Negro River near the Amazon River. The results suggest that the radiometric characteristics of sensors, like sentinel-2 MSI, are suitable for monitoring the suspended sediment concentration in large tropical black-water rivers.

Parameter Estimation of a Seasonal Poisson INAR(1) Model with Different Monthly Means

Analysing seasonality in count time series is an essential application of statistics to predict phenomena in different fields like economics, agriculture, healthcare, environment, and climatic change. However, the information in the existing literature is scarce regarding the performances of relevant statistical models. This study provides the Yule-Walker (Y-W), Conditional Least Squares (CLS), and Maximum Likelihood Estimation (MLE) for First-order Non-negative Integer-valued Autoregressive, INAR(1), process with Poisson innovations with different monthly means. The performance of Y-W, CLS, and MLE are assessed by the Monte Carlo simulation method. The performance of this model is compared with another seasonal INAR(1) model by reproducing the monthly number of rainy days in the Blackwater River watershed located in coastal Virginia. Two forecast-coherent methods in terms of mode and probability function are applied to make predictions. The models’ performances are assessed using the Root Mean Square Error and Index of Agreement criteria. The results reveal the similar performance of Y-W, CLS, and MLE for estimating the parameters of data sets with larger sample size and values of α close to unite root. Moreover, the results indicate that INAR(1) with different monthly Poisson innovations is more appropriate for modelling and predicting seasonal count time series.

Ontogenetic change in the diet of Semaprochilodus insignis (Characiformes: Prochilodontidae) during migration between two limnologically distinct environments in the Amazon Basin

Abstract Semaprochilodus insignis is an Amazonian migratory fish species, moving in large shoals between white- and black-water rivers. It has long been classified as a detritivorous fish. However, it is possible that the trophic plasticity of S. insignis could be higher than previously assumed. The objective of this study was to investigate the relative contributions of autotrophic energy sources to the diet of S. insignis in the Negro and Solimões rivers and to determine if the species undergoes an ontogenetic change in the diet. We found variations between the δ13C and δ15N values of S. insignis between the rivers. In the Negro River, periphyton (84%) was the principal energy source for adults, while juveniles in the Solimões River foraged predominantly on terrestrial plants/C3 macrophytes (50%) and phytoplankton (42%). These variations in isotopic signatures are likely associated with migratory movements of S. insignis at different life stages and hydrological periods. Instead of the previously assumed dietary classification, we suggest that S. insignis varies its diet ontogenetically, with adults acting as illiophagous in black-water while the young are detritivorous in white-water rivers. The results show that this species creates complex links between food chains, thus emphasizing the importance of conserving flooded areas.

Interaction of Pb, Ni and Cd with Aquatic Humic Substances of Amazonian Blackwater Rivers

In this study, the extraction and characterization of aquatic humic substances (AHS) from two blackwater rivers in the Amazon basin was carried out in order to assess the seasonal effects in the AHS structure. In addition, an investigation of the complexing capacities (CC) of the structures containing the metal ions CdII, NiII and PbII was also done. The characterization (of the AHS) was done using UV-Vis, molecular fluorescence, and infrared spectroscopy. AHS from the Negro River showed more aromatic characteristics in its structures and molecular weight, than the AHS from the Caru River. The AHS studied presented a seasonal pattern characterized by higher humification levels during the rainy season. For all the metals, the AHS complexing capacity was higher for the Negro River. The highest complexing capacities were observed for the NiII assays, which were 0.56 and 0.12 µmol NiII mg-1 dissolved organic carbon (DOC), for AHS RN and AHS RC-II, respectively. We concluded that the transport, bioavailability, and toxicity, of these metal species should be higher for Caru River.

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients.

Wastewater is considered a renewable resource water and energy. An advantage of decentralized sanitation systems is the separation of the blackwater (BW) stream, contaminated with human pathogens, from the remaining household water. However, the composition and functions of the microbial community in BW are not known. In this study, we used shotgun metagenomics to assess the dynamics of microbial community structure and function throughout a new BW anaerobic digestion system installed at The Netherlands Institute of Ecology. Samples from the influent (BW), primary effluent (anaerobic digested BW), sludge and final effluent of the pilot upflow anaerobic sludge blanket (UASB) reactor and microalgae pilot tubular photobioreactor (PBR) were analyzed. Our results showed a decrease in microbial richness and diversity followed by a decrease in functional complexity and co-occurrence along the different modules of the bioreactor. The microbial diversity and function decrease were reflected both changes in substrate composition and wash conditions. Our wastewater treatment system also decreased microbial functions related to pathogenesis. In summary, the new sanitation system studied here fosters microbial groups and functions that allow the system to efficiently and robustly recover carbon and nutrients while reducing pathogenic groups, ultimately generating a final effluent safe for discharge and reuse.

Sediment Deposition and Availability in Riparian Wetlands

Eulie, D.O.; Leonard, L., and Polk, M., 2021. Sediment deposition and availability in riparian wetlands. Journal of Coastal Research, 37(2), 302–315. Coconut Creek (Florida), ISSN 0749-0208.Coastal flooding and sea-level rise have the potential to negatively influence the ability of coastal wetlands to accumulate sediment, threatening the persistence of coastal wetlands. This study examines sediment availability, deposition rates, and elevation change in two types of tidally-dependent riparian wetlands to understand their ability to persist in the wake of rising sea level. Tidal marshes and riparian swamp habitats in blackwater and brownwater river systems were studied over a 32-month period between 2004 to 2007. Wetlands along the brownwater river system exhibited significantly greater and more variable deposition rates than those located along the blackwater river system (p < 0.05). The brownwater marsh lost –1.7 cm of elevation and the blackwater marsh lost –1.1 cm of elevation, while the brownwater swamp site had an increase of 7.0 cm and the blackwater swamp site exhibited an increase in elevation of 0.7 cm. Within the Cape Fear River Estuary, a combination of sediment availability, organic content, and wetland type (tidal brackish marshes, riparian freshwater swamp forests) are what most strongly influenced patterns of deposition but are strongly temporally and spatially influenced. Marshes in the Cape Fear River are losing elevation, while riparian swamps are, at the time of this study, maintaining their current elevation. Coastal tidal wetlands are under increasing pressure from climate change, sea-level rise, and urban development resulting in vertical drowning. Tidal marshes in the Cape Fear River are not able to maintain elevation, and riparian swamp forests may also be unable to as the magnitude of these pressures increase.

Functional Divergence between Várzea and Igapó Forests: A Study of Functional Trait Diversity in the Colombian Orinoco Basin

Research Highlights: Functional diversity studies help to better understand how organisms respond to different environmental conditions. Conditions in tropical flooded forests are highly variable, including levels of nutrient availability, pH, and flood depth, but few studies have explored the impact of variation in these factors on plant functional diversity. Background and Objectives: In the Orinoco basin, as in the Amazon, floodplain forests have been classified into várzea (white-water rivers, with nutrient-rich soils) and igapó (black-water rivers, associated with nutrient-poor soils). We evaluated the functional diversity of plant species in várzea and igapó, as well as the influence of external and internal filters on the plant community assembly of each forest type, and compared our results with studies in the Amazon basin. Materials and Methods: Six functional traits were recorded in the várzea and igapó forests of the Colombian Orinoco basin (one-hectare plot for each forest type, with no replicates). We evaluated plant species diversity (richness, Fisher’s α, Shannon and Simpson indices), as well as functional diversity (functional richness, functional evenness, functional divergence, and functional dispersion) and the influence of external and internal filters, based on a comparison of variance at different organizational levels. Results: A high functional differentiation between várzea and igapó was found, as well as a high functional divergence within each forest type. We also observed a greater influence of internal filters on the community assembly of both forest types, compared to external filters. Functional traits such as wood density and leaf dry matter content, showed the same patterns as the várzea and igapó forests in the Amazon. Conclusions: Despite the low taxonomic and functional richness, there is high functional divergence within flooded forests. We also show that in forests under stress (e.g., from flooding), internal filters can be key in assembling communities and promote high functional divergence. Given that the functional diversity of the várzea and igapó in the Orinoco is largely unexplored, we highlight the need for more research for the effective conservation of these flooded forests.

Coastal Marsh Degradation Into Ponds Induces Irreversible Elevation Loss Relative to Sea Level in a Microtidal System

AbstractCoastal marshes and their valuable ecosystem services are feared to be lost by sea level rise, yet the mechanisms of marsh degradation into ponds and potential recovery are poorly understood. We quantified and analyzed elevations of marsh surfaces and pond bottoms along a marsh loss gradient (Blackwater River, Maryland, USA). Our analyses show that ponds deepen with increasing tidal channel width connecting the ponds to the river, indicating a new feedback mechanism where channels lead to enhanced tidal export of pond bottom material. Pond elevations also decrease with increasing pond size, consistent with previous work identifying a positive feedback between wind wave erosion and pond size. These two positive feedbacks, combined with bimodal elevation distributions and sharp topographic boundaries between interior ponds and the marsh platform, indicate alternative elevation states and imply that marsh loss by pond formation is nearly irreversible once pond deepening exceeds a critical level.

New insights on the classification of major Amazonian river water types

Traditional water classifications for Amazonian rivers are based mainly on optical characteristics that do not fully capture their complexity. Today, an increasing amount of hydrochemical data indicates that the chemical composition of Amazonian rivers varies much more than this simple classification indicates. Revising river classification based on hydrochemical properties is proposed here. In this first comprehensive review of Amazonian river chemistry, we synthesized critical information from 168 scientific publications and distinguish unusual white, black, and clearwater rivers. It is shown the distribution of such rivers across the basin and the limitations of using generalized designations for river typologies. For example, some optically clearwater rivers draining carboniferous stripes have chemical properties similar to whitewater rivers, regardless of their high transparency. Furthermore, a clear or blackwater stream that becomes turbid because of soil erosion does not become a whitewater stream. Its hydrochemical parameters of acidity and nutrient poverty depend on the geology of the catchment area and remain relatively unaffected. These insights into Amazonian river classification provide a new understanding of their baseline limnological conditions. They have implications for sustainable management of freshwater systems and for monitoring potential impacts of large development projects and climate change on the Amazonian aquatic systems.

Molana Abbey: from medieval monastery to Tudor Manor to Georgian folly

Molana Abbey, a small Augustinian monastery near Youghal, Co. Cork, became the property of Sir Walter Raleigh as part of the Elizabethan colonization of Munster. It passed from him to his scientific advisor, Thomas Harriot, and later via Richard Boyle, Great Earl of Cork, into the Smyth family, who held it until the end of the 20th century. After initial observations of the ruins, a three-stage research project of architectural survey, mortar analysis, and archaeological testing took place from 2012 to 2015. This paper presents those findings, which point to post-medieval alterations and decay as well as systematic changes made to create a medieval folly for the grand Georgian mansion, Ballynatray House, on the banks of the Blackwater River.

Reproductive biology of Neotropical tetra fish Mimagoniates lateralis (Nichols, 1913) (Characiformes, Characidae), an endangered species of blackwater streams of southeastern Brazil

ABSTRACT This study provides for the first time information about the reproductive biology of Mimagoniates lateralis, an endangered species of inseminating Neotropical tetra that occurs in blackwater streams of southeastern Brazil. We analyzed 404 individuals, of which 214 were females, 117 were males, and 73 were immature juveniles. The sex ratio was 1.83 females/males. Males were larger than females. The estimated size at first maturation for females was 22.2 mm SL, and for males 23.4 mm SL. We also analyzed 94 ovaries from mature females, and the estimated absolute fecundity was 116.37 ± 26.68 oocytes, the oocyte diameter varying from 0.1 to 1.0 mm. The spawning type was classified as multiple, with two modal values corresponding to different oocyte lots. These data contribute to substantiate future assessments of the conservation status of M. lateralis, as well as future attempts to guarantee the preservation of this species and its natural habitat.