Tobacco streak ilarvirus (TSV) seed transmission was investigated by reciprocal pollinations as well as by serological and infectivity assays of flower parts from beans (Phaseolus vulgaris) systematically infected with either TSV pathotype I (isolate Mel 40) or TSV pathotype II (isolate Mel F). Heathly and TSV-infected Black Turtle Soup (BTS) bean plants were reciprocally pollinated by using anthers from either heathly plants or plants systematically infected with Mel 40 or Mel F. When anthers from plants infected with Mel 40 were used to pollinate heathly plants, a high percentage of the resulting seedlings were infected with that isolate (...)