Sesame Research Articles

Effects of priming duration and rhizosphere bacteria metabolite concentration on the germinability of cowpea, soybean, sesame, and okra seeds

Seed priming enhances germination and growth, which are important determinants of crop yield. This study was carried out to assess the effect of priming duration and metabolite concentration on the priming of five (5) crops using the metabolites of five (5) bacterial isolates. The crop seeds were treated in the cold-extracted metabolites of the five isolates at five (5) different priming durations (1, 2, 3, 4, and 5 h) and then in five metabolite concentrations (200, 400, 600, 800, and 1000 mg/L) of the five extracted metabolites at the optimal priming duration determined in the first experiment. Characterization of the cold-extracted metabolites was also carried out using gas-chromatography-mass spectrometry (GC-MS). Results revealed that priming cowpea and soybean seeds for longer durations (< 3 h) could hinder their growth and development. Lower concentrations were observed to be optimal for cowpea and soybean, but for sesame and okra, there was no detectable pattern with metabolite concentration. The GC-MS revealed the presence of some molecules (e.g. hexadecanoic acid) that have shown plant growth promotion potential in other studies. This study showed that seeds with large endosperms, such as those of cowpea and soybean, are more prone to the harmful effects of treatment for longer durations. Further experiments should be undertaken to isolate and purify the bioactive moieties for further studies and application.

Seed quality and relative lignan profiles of sesame prospected from northern Ghana

The sesame seed contains oil, protein, dietary fibre, and several minerals and it is also a store of lignans. Lignans are key selection factors for sesame quality due to their health, nutritive and market value. In Ghana sesame growers rely on wild or undocumented planting seeds which are of mixed colouration and its lignan content is ambiguous. The objective of this study was to segregate street sesame seeds into component colour fractions and subsequently evaluate the consistency of lignans in the seed fractions. Sesame seeds were collected from street vendors in northern Ghana and were segregated into seed fractions based on the pericarp colour. The viability of seed fractions stored at different temperatures (ambient, 5 °C, 0 °C) over time and lignan contents of single or bulk mixed seeds were verified. The collected seeds were of mixed colouration with approximately 4 % debris, 40 % white, 36 % cream, and 20 % dark coloured seeds by weight. The viability of the seeds was 67–85 % depending on pericarp colour. White seed fractions which had the highest proportion by weight had a significantly (p = 0.0275) higher viability (85 %) than the dark seeds which had the least viability (67 %). The seeds lost viability over time. However, seeds stored at 0 °C maintained a viability of 77 % at the fourth year. There were differences in the relative abundance of lignans for both bulk seeds and, single seeds with different pericarp colours. The most abundant occurring lignans in the seeds were sesamin, sesamolin and sesaminol and its downstream glucosides. The source of seed or pericarp colour was not predictive of seed viability or lignan composition. These findings provide baseline data on seed quality including an improved storability under cold environments. It also gives an insight into how mixed seeds of variable pericarp colours can have distinct characteristics. Although the mixed coloured seeds had no implications for varied quality including lignan content, the international market for sesame requires that seeds are of consistent colour.

Identifying food safety risks with a novel digitally-driven food safety early warning tool – A retrospective study on the pesticide ethylene oxide

Foodborne diseases present a major global health challenge, with 600 million annual cases and 420,000 deaths worldwide in 2023 reported by the World Health Organization, underscoring the critical need for early risk detection and swift measures. Novel solutions for early risk detection in food are emerging due to artificial intelligence-based data models. By applying diverse algorithms and ontologies in data processing, the continually expanding amount of available data can be harnessed in a precise manner. To assess the efficacy of such technologies in early risk detection, we evaluated whether the risk to exceed a legal limit for ethylene oxide – specifically in sesame seeds – could have been identified sooner with the assistance of a commercially available Artificial Intelligence (AI)-based platform. The non-compliance of sesame seeds led to various product recalls in the year 2020. The result of this retrospective case study shows that the first indirect indications of the issue started to emerge from the year 2018 with initial Rapid Alert System for Food and Feed (RASFF) notification of ethylene oxide limit value exceedances in black pepper powder. Based on these promising findings, the subsequent challenge is to develop a methodology for systematically categorizing and evaluating similar risks in the light of the exponentially growing volume of accessible data.

Genetic variability, trait association and path coefficient analysis for yield and yield attributing traits in sesame (Sesamum Indicum L.)

Genetic variability, trait association and path coefficient analysis for yield and yield attributing traits in sesame (Sesamum Indicum L.)

Biochemical characterization of sesame (Sesamum indicum L.) varieties grown in Togo

Food and nutrition insecurity are a major problem in low-income countries. However, some crops like sesame are still underused and can be used to improve the food and nutritional security in rural areas. Grains of seven varieties of sesame comprising four (4) local and three (3) introduced varieties were studied for their nutrient content and oil composition to provide information on their nutritional value and oil quality for a holistic development and valorisation of the crop in Togo. Results revealed that water, ash, lipid, protein, total sugar, fiber, vitamin C, energy, total phenol and tannin contents ranged from 5.08 to 6.30%; 3.94 to 5.40%; 36.69 to 45.62%; 19.52 to 25.37; 11.10 to 19.16; 3.84 to 7.01%; 1.10 to 1.94%; 660 to 953.3 Kcal/ 100g; 0.0020-7 to 0.006% and 0.005 to 0.017%. The oils extracted from these different varieties had saponification and acid indices that ranged respectively from 179.95 to 190.54 and from 4.47 to 8.96. In terms of fatty acid composition, oleic acid and linoleic acid ranged from 37.92 to 42.24% and 36.1 to 39.8% respectively, while palmitic acid ranged from 6.82 to 12.41% and stearic acid from 5.51 to 7.83%. The results enabled us to identify the sesame grains of local varieties G3 and G3B as being suitable for enriching infant flours or for remedying the problems of protein and energy deficiency in Togo. Further, the oils extracted from these varieties are of good quality and can therefore be used in industry and for food purposes.

Application of Artificial Neural Networks for Predicting Cooking Dynamics in Industrial Sesame Seed Oil Extraction

Application of Artificial Neural Networks for Predicting Cooking Dynamics in Industrial Sesame Seed Oil Extraction

Biosynthetic Sesame Oil and Nano Titanium Dioxide As a Therapy For Second Degree Burns

As the healing of burn wounds delay that affect the skin and lead to tearing and damage to its parts, as well as the remaining traces and scars on the skin because of exposure to heat, radiation, radioactivity, electricity, friction, or contact with chemicals, it has become necessary to investigate and discover effective medicines. Burns that affect the skin have an important purpose, Therefore, it is important to continue studying and experimenting with new and effective medicines, including effective nanomaterials, to reach efficient treatments to treat such injuries. In the current study, nanoparticles of titanium dioxide (TiO2) were prepared, and this material was examined using a device a Field Emission Electron Scanning Microscope (FE-SEM). The results obtained were on the nanoscale, with sizes ranging from (31.52-69.08) nanometers. The effectiveness and effect of biosynthetic nanomaterials and sesame seeds were investigated in vivo at a concentration of 1% TiO2 and 20% sesame oil on burns in the skin of laboratory rats and compared with a control group of natural skin of the same type of laboratory rats. They were also compared with rats treated with Mebo ointment. Randomly, 18 rats were put into three equal groups and treated once daily for 28 days. The results of the study showed effective activity and a noticeable speed in the healing of burns that affected the skin of laboratory rats using a nano-titanium dioxide preparation supported by sesame seed extract whose burn mean amounted to 0.005 compared to the control treatment, whose burn mean was 2.443. According to the current study's findings, using biosynthetic nano-titanium dioxide and sesame oil together results in better burn wound healing than using each of them separately.

Exploring the allergenic potential of sesame oleosins: Isolation and bioinformatics analysis

This study examined two oleosins of 17 kDa and 15 kDa isolated from Yuzhi white sesame seeds through oil body extraction. The allergens were identified as oleosin H1 (Ses i 4) and oleosin L (Ses i 5) using SDS-PAGE, dot blot analysis, and LC-MS/MS. PCR analysis revealed high sequence homology for the oleosin proteins in the sesame seeds. Utilizing AlphaFold2, bioinformatics tools, and protein-protein docking, the structure and function of these oleosins were analyzed. Ten potential B cell epitope peptides were predicted and mapped onto the α-helix and random coil-dominated oleosome membrane conformation. IgE binding simulations identified key epitopes, B3 (FLTSGAFGL) and B4 (KRGVQEGTLY) for oleosin H1, and B8 (GGFGVAALSV) and B9 (DQLESAKTKL) for oleosin L. Mutational analysis highlighted Glu135, Phe102, Tyr128, Tyr139, Gly136, and Gly132 in oleosin H1, and Leu120, Lys119, and Leu113 in oleosin L as critical residues for binding stability, providing insights into the sensitization mechanism of these epitopes. The integration of bioinformatics and immunoinformatics in this study has contributed to a deeper understanding of the allergy properties of sesame oleosins.

High-density genetic map construction and QTL mapping to identify genes for blight defense- and yield-related traits in sesame (Sesamum indicum L.).

Sesame (Sesamum indicum L.) is an important oilseed crop widely cultivated in subtropical and tropical areas. Low genetic yield potential and susceptibility to disease contribute to low productivity in sesame. However, the genetic basis of sesame yield- and disease-related traits remains unclear. Here, we represent the construction of a high-density bin map of sesame using whole genome sequencing of an F2 population derived from 'Yizhi' and 'Mingdeng Zhima'. A total of 2766 Bins were categorized into 13 linkage groups. Thirteen significant QTLs were identified, including ten QTLs related to yield, two QTLs related to Sesame Fusarium wilt (SFW) disease, and one QTL related to seed color. Among these QTLs, we found that SFW-QTL1.1 and SFW-QTL1.2 were major QTLs related to Fusarium wilt disease, explaining more than 20% of the phenotypic variation with LOD > 6. SCC-QTL1.1 was related to seed coat color, explaining 52% of the phenotypic variation with LOD equal to 25.3. This suggests that seed color traits were controlled by a major QTL. Candidate genes related to Fusarium wilt disease and seed color in the QTLs were annotated. We discovered a significant enrichment of genes associated with resistance to late blight. These genes could be spectral disease resistance genes and may have a role in the regulation of Fusarium wilt disease resistance. Our study will benefit the implementation of marker-assisted selection (MAS) for the genetic improvement of disease resistance and yield-related traits in sesame.

Comparative growth, biochemical and antioxidative response of Glycine max and Sesamum indicum to MnS nanoparticles

Manganese is an important micronutrient that affects plant growth and development. This study proved that Manganese Sulfide nanoparticles (MnS NPs) are a better alternative to manganese salt (MnSO4) for the growth of Sesamum indicum and Glycine max under in vivo conditions. SEM, FTIR, EDX and, XRD revealed spherical, pure and, 8 nm size of MnS NPs. The addition of MnS NPs to the soil increased root and shoot length, number of branches, biomass, and protein contents in a dose-dependent manner up to 40 mg/kg in both G. max and S. indicum. Atomic Absorption Spectroscopy (AAS) showed bioaccumulation of Mn at 103.8 and 50.4 mg/kg DW in G. max root and shoot respectively at 80 mg/kg NPs soil and, 127.6 and 84.6 mg/kg DW in S. indicum root and shoot respectively at 80 mg/kg NPs soil. NPs application leads to the activation of antioxidant enzymes (SOD, POD) and the synthesis of secondary metabolites (total phenolics and flavonoids) in a concentration-dependent manner. The extract obtained from NPs treated plants showed higher total antioxidant capacity (TAC), total reducing power (TRP), and DPPH free radical scavenging activity. The hierarchical cluster analysis indicates 40 and 60 mg//kg soil NPs to be the stimulant of plant growth and alkaloid production. The results show that MnS NPs application influenced the plant biochemical profile, and enzymatic and non-enzymatic antioxidants in a dose-dependent manner. Therefore, MnS NPs can be novel elicitors to improve plant growth and biological contents.

Effect of thermal pretreatments on the quality attributes and irradiation markers of sesame oil extracted from sesame seeds without and with gamma irradiation

This study comparatively studied the effects of three thermal pretreatment methods, i.e., wet-heat (WT), roasting (RT) and microwave (MT), on the quality attributes and irradiation markers of sesame oil obtained from sesame seeds without and with gamma irradiation. Results showed that gamma irradiation had negligible effect on the quality of sesame seeds and their extracted oils. The effects of thermal pretreatments on irradiated and non-irradiated sesame seeds and their oils were similar, little synergistic effects were observed. The RT-treated oils had more carotenoids, chlorophyll, total phenols, tocopherols, and heterocyclic volatiles content, as well as longer oxidation induction time, but darker color compared with their WT- and MT-treated counterparts. All oil samples had identical FTIR spectra. Eight radiolytic hydrocarbons were identified in the irradiated sesame oils. Thermal pretreatments reduced the content of radiolytic hydrocarbons, but did not significantly change their composition. Our study helps to identify products from irradiated sesame seeds.

Sesame, an Underutilized Oil Seed Crop: Breeding Achievements and Future Challenges.

Sesame seeds and their edible oil are highly nutritious and rich in mono- and polyunsaturated fatty acids. Bioactive compounds such as sterols, tocopherols, and sesamol provide significant medicinal benefits. The high oil content (50%) and favorable mono- and polyunsaturated fatty acid balance, as well as resilience to water stress, make sesame a promising candidate crop for global agricultural expansion. However, sesame production faces challenges such as low yields, poor response to agricultural inputs, and losses due to capsule dehiscence. To enhance yield, traits like determinate growth, dwarfism, a high harvest index, non-shattering capsules, disease resistance, and photoperiod sensitivity are needed. These traits can be achieved through variation or induced mutation breeding. Crossbreeding methods often result in unwanted genetic changes. The gene editing CRISPR/Cas9 technology has the potential to suppress detrimental alleles and improve the fatty acid profile by inhibiting polyunsaturated fatty acid biosynthesis. Even though sesame is an orphan crop, it has entered the genomic era, with available sequences assisting molecular breeding efforts. This progress aids in associating single-nucleotide polymorphisms (SNPs) and simple sequence repeats (SSR) with key economic traits, as well as identifying genes related to adaptability, oil production, fatty acid synthesis, and photosynthesis. Additionally, transcriptomic research can reveal genes involved in abiotic stress responses and adaptation to diverse climates. The mapping of quantitative trait loci (QTL) can identify loci linked to key traits such as capsule size, seed count per capsule, and capsule number per plant. This article reviews recent advances in sesame breeding, discusses ongoing challenges, and explores potential strategies for future improvement. Hence, integrating advanced genomic tools and breeding strategies provides promising ways to enhance sesame production to meet global demands.

Investigation of biological activities of in vitro grown Sesamum orientale plant extract on the cell cultures: wound healing and antiproliferation

Investigation of biological activities of in vitro grown Sesamum orientale plant extract on the cell cultures: wound healing and antiproliferation

Tracking the storage stability in sesame (Sesamum indicum L.): impact of accelerated storage on storability characteristics, seed quality, phytochemical content, and fatty acids

Storage stability under non-optimal conditions is an essential characteristic of Sudanese sesame. To understand opportunities to improve storage stability in sesame, seed quality, storability characteristics, content of fatty acids and phytochemicals, and antioxidant capacity were evaluated in ten Sudanese genotypes subjected to high temperature (55 °C) and humidity (60% RH) for 16 and 32 days. The accelerated storage increased seed color, linoleic acid, fungal growth, and peroxide value, while oil content, oleic acid, water activity, phytochemicals, and antioxidant capacity decreased (P < 0.05). The germination rate and content of saturated fatty acids were retained despite the storage (P > 0.05). The landrace Abusundoug showed better storage stability than the other genotypes due to generally low fatty acids and high phytochemical contents. The differences in storage stability in the Sudanese genotypes underscore the need for their further evaluation and use in breeding programs to improve sesame shelf life and quality.

Evaluation of Agronomic Traits, Total Phenolic Content, and Antioxidant Properties of Sesame Seeds of Different Colors and Origin.

Rising health concerns regarding chronic diseases call for exploring natural sources of antioxidants and factors that influence their activity. This study evaluated the diversity of 112 sesame germplasms from Africa and Asia based on ten agronomic traits (seven quantitative and three qualitative), two antioxidant activities (ABTS and DPPH radical scavenging activities), and the content of one metabolite (TPC). TPC, DPPH, and ABTS were in the ranges of 4.98-87.88 µg GAE/mg DE, 3.97-46.23 µg AAE/mg DE, and 3.42-176.01 µg TE/mg DE, respectively. Statistical analyses revealed significant variations in agronomic traits, TPC, and antioxidant activities among the sesame germplasms (p < 0.05). Furthermore, the individual and interaction effects of seed color and the continent of origin on the levels of the quantitative traits, TPC, ABTS, and DPPH were analyzed, and the correlation among the traits was further evaluated. Diversity in TPC, ABTS, and DPPH was significantly associated with seed color and most of the quantitative agronomic traits (p < 0.05) but not with continent of origin. Principal component analysis revealed TPC, ABTS, DPPH, and five quantitative traits as the most discriminant traits. In general, six sesame accessions with high TPC and antioxidant activities (IT194356, IT170094, IT29971, IT185998, IT104246, and IT169623) as well as important agronomic traits were identified and, hence, could be used for developing improved sesame varieties.

Phytochemical and Ethnopharmacological Review of Till Safeed (Sesamum indicum Linn.)

Within the management of health care, the traditional system of medicine remains indispensable. The Unani system of medicine treats a variety of illnesses with a range of medications derived from plants. Historically, people have utilized sesamum indicum seeds as a medicinal ingredient. Various parts of the plant have been used by traditional physicians. However, the dried seed and oil are commonly used as Till in Unani Medicine. The plant has a very long history of use as a medicinal herb. It can be used on its own or in compound pharmacopeial compositions with other medications. Till Safeed is an annual plant of family Pedaliaceae and this review article aims to describe morphological characteristics, phytochemistry, ethnobotanical uses and therapeutic properties of Till Safeed. So that subsequent study on any novel therapeutic activity based on phytochemistry can be conducted with greater simplicity and to support the revalidation of the drug's scientifically claimed actions as mentioned in classical literatures. We systematically searched classical Unani literature, online data sources (PubMed, Google scholar, Elsevier, Science Direct and Research Gate) and offline encyclopaedia and books on medicinal plants for the relevant data on Till Safeed. Polyunsaturated fatty acids, phytosterols, tocopherols, essential minerals, and a special class of phenylpropanoid chemicals called lignans—which include sesamin, sesamol, and sesamolin—are among the bioactive ingredients found in till seeds. The pharmacological characteristics of sesame lignans include. Antioxidant, antibacterial, antiproliferative, cholesterol-lowering, hepatic fatty acid oxidation enzyme-increasing, and anti-hypertensive properties. Keywords: Sesamum indicum, Unani Medicine, phytochemistry, sesamin, sesamolin.

Enhancing Functional Compounds in Sesame Oil through Acid-Soaking and Microwave-Heating of Sesame Seeds.

This study investigated whether pre-treating sesame (Sesamum indicum L.) seeds with a combination of acid-soaking and microwave-heating could significantly enhance the quality of the resulting sesame oil, particularly by increasing its content of functional compounds such as lignans, tocopherol, phytosterol, and squalene. The study revealed that soaking the sesame seeds in a solution of HCl and citric acid, along with microwave-heating, significantly increased the content of these compounds. The detected ranges were sesamin (1365-6927 µg g-1), sesamolin (605-3493 µg g-1), tocopherol (69.31-282.76 µg g-1), asarinin (ND-383.52 µg g-1), sesamol (ND-49.59 µg g-1), phytosterol (3690-6201 µg g-1), and squalene (532-1628 µg g-1). Additionally, the study found that the pre-treatment of sesame seeds had a minimal effect on the fatty acid composition, antioxidant activity (92.94-95.08% DPPH scavenging activity), and oxidative stability (2.13-2.90 mg MDA kg-1 oil). This is the first study to demonstrate that using acid-soaking and microwave-heating to prepare sesame seeds can produce sesame oil enriched with functional compounds, potentially benefiting cosmetic, pharmaceutical, and health applications.

Ameliorating effect of Tennetti-Tilla (dried figs and sesame seeds laddu) on type 2 Diabetic convalescents-A case study

Diabetes mellitus is a common endocrine disorder of impaired glucose metabolism and is increasing globally due to changes in the lifestyle of individuals. It can be controlled or reversible by following a regular diet and proper exercises. Along with the diet exercises naturally available supplements are also helpful in controlling diabetes. There are many plant-based products which can be consumed in daily diet which act as nutraceuticals. The present case study was based on the natural supplementation with dried figs and sesame seeds. A 64-year-oldman known case of Type 2 diabetes consulted at nature cure hospital with chief complaint of uncontrolled fasting sugar and HbA1c since 8 years. Using dried figs and sesame seeds named as the formulation Tennetti-tilla. Along with regular medicines 10 g of supplementation was advised for 90 days to the subject. After the study period it was observed that Fasting sugar levels have reduced from 116 mg/dl to 96 mg/dl, post prandial 147mg/dl to 118 g/dl, HBA1c 7.1% to 6%.

Evaluation of Five Sesame (Sesamum Indicum L.) Varieties Under Varying Fertilizer Rates and Establishment Methods for Growth and Yield in Anyigba, Kogi State, Nigeria

Global sesame seed market is projected to have USD 7.24 billion in consumption value by 2024. However, its supply has not been able to match the demand due to several constraints to its production. This study was conducted at the Prince Abubakar Audu University Research and Demonstration Farm, during the 2023 cropping season to evaluate five sesame varieties for growth and yield under varying fertilizer rates and establishment methods. Treatment consisted of five sesame varieties, two establishment methods, and three fertilizer levels respectively combined factorially to obtain a total of thirty factorial treatment combinations and laid out in a Randomized Complete Block Design (RCBD) with three replicates. Results showed that varieties differed significantly (P &lt; 0.05) in growth, yield and yield characters. YANDEV55 produced the widest leaf area at 9WAS, NCRIBEN-02M produced the highest number of leaves per plant (36) at 12WAS. The same variety (NCRIBEN-02M) consistently produced the tallest stems (42.48, 56.10, 80.48 &amp; 93.46 cm) at 3, 6, 9 &amp; 12WAS. YANDEV55 produced 73.6 % germination which was highest amongst others. E8 produced the highest number of capsule/plant (23.3) and number of seeds/plant (1428.94). NCRIBEN-05E performed optimally in yield characters such as total plant biomass yield (2908.0g/plot) and final seed yield (887.9kgha-1). NCRIBEN-02M had the highest haulm yield (33.0g/plot) while NCRIBEN-01M had the longest days (54.16) to 50% anthesis. Seed/capsule and Harvest index were not significantly affected by varieties. Broadcast method produced significantly widest leaf areas of 53.9 and 40.5 cm2 at 6 and 12 WAS respectively, higher number of leaves (18.0) at 3WAS. Drilling method of crop establishment produced the highest biomass yield (2894.6g/plot) and final seed yield (879.2 kgha-1). Similarly, fertilizer rates affect growth and yield characters; 100 kg NPKha-1 produced the widest leaf area, tallest plants and highest number of leaves/plant at all stages of sampling. These were at par with values obtained when plots were treated with 150kg NPKha-1. Alternatively, highest number of capsules/plant (22.4), seeds/capsule (62.46), seeds/plant (1502.73), total plant biomass yield (2879.7g/plot), haulm yield (33.0g/plot), final seed yield (870.7kgha-1) and longest number of days to 50% anthesis (53.6) were all obtained when plots were treated with 150 kgha-1 but was at par with 100kg NPKha-1. Fertilizer rates did not affect the establishment count and harvest index. The best variety with respect to yield/ha is NCRIBEN-02M, Application of 100 kg NPK/ha appears maximum for optimum yield of the crop, drilling appears to produce higher yield than broadcast for some varieties with an overall marginal increase of 8.4 % over broadcast and thus recommended for Anyigba environment. An economic study of the cost implication for both operations (drilling and broadcast) could be embarked upon to ascertain the veracity of this assertion, more so that broadcast requires higher seed rate than drilling. Significant interaction of varieties x establishment method was also an indication that very high yielding varieties (NCRIBEN-02M and NCRIBEN-01M) do better when drilled compared to broadcast. Significant positive relationship between some growth, yield and yield characters is an indication that effective crop improvement through selection can be carried out by selecting for those characters that correlated positively with yield.

Study on the Optimization of Process Conditions for the Development of Purple Rice Milk Product

The purpose of this study was to study the development of purple rice milk products, which aimed 1) to study on the optimization for digestion of starch using Amylase enzymes and 2) to evaluate the sensory of rice milk products. In the experiment designed of the optimization was used two factors include time with 35, 40, 45, 50 mintue and enzymes concentation 0.05%, 0.1%, 0.15% and 0.2% and using CCD (Central Composite Design) and RSM (Response Surface Methodology) were investigated the optimum condition which results in the analysis of two factors with duration of hydrolysis was 43 minute and concentration of enzymes was 0.141% consisted high value of total soluble solid (TTS) 10.33 and SD value of 0.57 with R2 85.152%. For the sensory evaluation was used QDA (Quantitative Descriptive Analysis) method, which is an evaluation of the characteristics of the product from the tester. The formulation of rice milk was divided into 5 formulas. In determining the specific characteristics of the product was set at 16 specify Characterized by the main characteristics include: odor, taste, texture and after tasting. The sensory evaluation results showed that: Formula 2 consisted of rice odor, sugar odor, caramel odor, sweet, soft, liquid, tasted in the mouth and refreshing. Formula 4 consisted of a pungent, sugary, aromatic, caramel, sweet, soft, liquid, mouth-watering and refreshing taste, and The results of the analysis was used PCA which analyzed priciple component (PC) with a confidence level of 85.57%. As for the preference percentage of the product obtained from Preferance mapping analysis the result be seen that the preference percentage of all 5 formulations were as follows: Formula 2 rice 50% with soybean 50% and formula 4 rice 50% with peanut 50% accouting for 60%-80%, After analyzing the data to find the difference, it was found that the raw materials used in purple rice milk in formulas 2, 3, 4 and 5 consisted of purple rice, soybeans, sesame seeds and peanuts were significantly affect to charateristic and preferance of the products