AbstractThe minicolumn (MC) proposed by Holaday and Lansden was developed with standard aflatoxin solution and also with the extracts of corn, rice, wheat, cottonseed, peanut cake and black pepper; each having different levels of aflatoxins. One‐half mL each of 2,4‐dinitrophenylhydrazine,p‐anisaldehyde, 20% H2SO4, 20% HCl and trifluoroacetic acid (TFA) with 25% HNO3, which were used for confirming aflatoxins on TLC, were applied to the developed column. Among these, all the 3 acid reagents changed the blue fluorescence of aflatoxins to yellow and thus were found to be satisfactory confirmatory tests. The TFA with 25% HNO3 had the lowest detection limit‐5 ppb.