The MoBiMet (Mobile Biometeorology System) is a low-cost device for thermal comfort monitoring, designed for long-term deployment in indoor or semi-outdoor occupational contexts. It measures air temperature, humidity, globe temperature, brightness temperature, light intensity, and wind, and is capable of calculating thermal indices (e.g., physiologically equivalent temperature (PET)) on site. It visualizes its data on an integrated display and sends them continuously to a server, where web-based visualizations are available in real-time. Data from many MoBiMets deployed in real occupational settings were used to demonstrate their suitability for large-scale and continued monitoring of thermal comfort in various contexts (industrial, commercial, offices, agricultural). This article describes the design and the performance of the MoBiMet. Alternative methods to determine mean radiant temperature (Tmrt) using a light intensity sensor and a contactless infrared thermopile were tested next to a custom-made black globe thermometer. Performance was assessed by comparing the MoBiMet to an independent mid-cost thermal comfort sensor. It was demonstrated that networked MoBiMets can detect differences of thermal comfort at different workplaces within the same building, and between workplaces in different companies in the same city. The MoBiMets can capture spatial and temporal differences of thermal comfort over the diurnal cycle, as demonstrated in offices with different stories and with different solar irradiances in a single high-rise building. The strongest sustained heat stress was recorded at industrial workplaces with heavy machinery.