Black Cumin Seed Oil Research Articles

Antibacterial Effects of Black Cumin Seed Oil on Oral Microcosm Biofilms.

Interest in natural extracts for managing oral biofilms is increasing, with black cumin seed oil (BCSO) demonstrating efficacy against Streptococcus mutans. The effectiveness of antibacterial agents should be evaluated using multi-species oral biofilm models that closely mimic actual conditions. This study aimed to compare the antibacterial effects of BCSO and chlorhexidine gluconate (CHX) on oral microcosm biofilms. Biofilms using human saliva as the inoculum were cultured for 2 days and subsequently treated with 0.5% dimethyl sulfoxide, 0.5% BCSO, or 0.12% CHX once daily for 6 days. Following treatment, the red fluorescence intensity (RatioR/G) of the oral biofilm; biomass, including extracellular polymeric substance (EPS) levels and live bacteria counts; and colony-forming units (CFUs) of aciduric bacteria were evaluated. RatioR/G after BCSO treatment (1.26 ± 0.03) was not significantly different from that after CHX treatment (p = 0.552). The EPS levels were also not significantly different between the two groups (p = 0.743). The live bacteria count was 0.55 times lower in the BCSO-treated group than in the CHX-treated group (p = 0.018). No significant between-group difference was observed in the CFUs of aciduric bacteria (p = 0.935). These results suggest that BCSO exhibits antibacterial effects similar to those of CHX, highlighting its potential as an effective alternative.

Antiviral activity of Nigella sativa oil against two SARS-CoV-2surrogates in vitro as a dietary product

Background: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is primarily transmitted via aerosol and droplets, but environmental surfaces or potentially contaminated foods may contribute to further viral transmission. Nigella sativa seed (black cumin seed) oil possesses various biological activities against bacteria, fungi, parasites, orviruses. Objectives: This study aimed to explore black cumin seed oil (BSO) with anti-SARS-CoV-2 activities using bovine coronavirus (BCoV) and human coronavirus (HCoV) OC43 as surrogates. Methods and Results: BSO was tested at 1-100 mL/L concentrations for cytotoxicity towards human rectal tumor (HRT-18G) cells and 0.1-10 mL/L for efficacy against BCoV and HCoV OC43 in suspension. Two coronaviruses were separately mixed with BSO suspension and incubated for various times (0, 15, 30, 45, and 60 min) at 4, 23, and 37°C. The virus titerreduction was determined by median tissue culture infectious dose (TCID50) assay with HRT-18G cells as the host. BSO concentrations of 1-20 mL/L exhibited low cytotoxicity (<10%) towards HRT-18G cells. The inactivation of coronaviruses by BSO was concentration-, temperature-, and exposure time-dependent. At 37°C, BSO reduced 2.0 logs of BCoV and3.0 logs of HCoV OC43 in 60 min, respectively. Specifically, half maximal effective concentration (EC50) of BSO at 4, 23, and 37°C after the 1-h exposure was 4.28, 3.06, and 0.87 mL/L against BCoV, respectively, and 0.61, 0.61 and 0.48 mL/L against HCoV OC43, respectively. Moreover, BSO didn’t affect the attachment of BCoV and HCoV OC43 into host cells. Conclusion: Our findings on the inhibition activity of BSO against SARS-CoV-2 surrogates suggested the potential use of this natural product in mitigating SARS-CoV-2 infection. Our results also suggest that HCoV OC43 may be a more appropriate surrogate for SARS-CoV-2 to screen antiviral dietary products. Keywords: Nigella sativa, SARS-CoV-2 surrogates, antiviral activity, quantitative suspension test, dietary supplement

Black cumin seed oil and its nano-form ameliorate lipopolysaccharide-induced brain inflammatory injury in mice

Backgrounds and objectives Microglia play a regulatory role in central nervous system inflammatory diseases, such as Alzheimer’s, Parkinson’s, and multiple sclerosis. Natural remedies like black cumin seeds (Nigella sativa) are rich in bioactive compounds that potentially can modulate inflammatory processes in the brain. In the current work, we studied the protective and anti-inflammatory properties of black cumin seed oil (BCSO) and its nano-form on lipopolysaccharide (LPS)-induced neurotoxicity in mice. Materials and methods Forty-eight mice were divided randomly into eight groups (n=6), three control groups (negative control, BCSO control, nano-BCSO control), LPS group, and four treatment groups [BCSO+LPS, nano-BCSO+LPS, indomethacin (5 mg/kg)+LPS, BCSO+indomethacin(2.5 mg/kg)+LPS]. At the end of the experiment, the brain tissues were removed for histopathological and biochemical assessments. Malondialdehyde and interleukin (IL)-10 were assessed using enzyme-linked immunosorbent assay while the gene expression of IL-6, toll-like receptor-4, brain-derived neurotrophic factor, nerve growth factor, cyclooxygenase-2, and B-cell lymphoma-2 were assessed by real-time PCR. IL-1β was quantified immunohistochemically along with the histopathological studies of the cerebral cortex of mice brains. Results and conclusions In our study, BCSO and its nano-form demonstrated a reduction in LPS-induced neurotoxicity, exhibiting comparable or better anti-inflammatory effects to indomethacin. These treatments significantly elevated the gene expression levels of neuroprotective factors brain-derived neurotrophic factor and nerve growth factor in LPS-treated mice. Pretreatment with BCSO and its nano-form reduced the malondialdehyde levels, in addition to gene expressions of cyclooxygenase-2, toll-like receptor-4, IL-6, and B-cell lymphoma-2. Immunohistochemical analysis indicated a decrease in IL-1β with BCSO and the lowering effect of the nano-form was superior. The histopathological studies corroborated with biochemical and molecular findings, suggesting that BCSO and its nano-form attenuated the inflammation and enhanced the microglial antioxidative and anti-inflammatory status. BCSO could enhance the anti-inflammatory activity of indomethacin, so lower doses of indomethacin with BCSO may be suggested for protecting against the adverse effects of high doses of NSAIDs as gastritis. Consequently, BCSO can serve a potential stimulatory supplement of the immunity for neurodegenerative conditions.

Preparation and Application of Black Cumin Seed Oil Emulsion with Enhanced Stability for Antimicrobial Treatment of Cellulosic Fabric

Preparation and Application of Black Cumin Seed Oil Emulsion with Enhanced Stability for Antimicrobial Treatment of Cellulosic Fabric

Effects of temperature and pressure on volatile compounds of black cumin seeds (Nigella sativa L.) oil extracted by supercritical carbon dioxide

AbstractThis study investigated the effects of temperature and pressure on the yield and volatile compound contents of black cumin seed oil (BCO) extracted from black cumin seeds using supercritical carbon dioxide (SCCO2). The solubility of BCO in SCCO2 increased 2.5‐fold as the pressure increased by 10 MPa. Major volatile compounds in BCO were identified using a static headspace gas chromatography–mass spectrometry (SH–GC–MS). Evidence indicated the main volatile components in SCCO2‐extracted BCO to be hexanal, p‐cymene, and thymoquinone. BCO extracted from China‐cultivated black cumin seed contained similar amounts of p‐cymene and thymoquinone. This black cumin seed is classified as the cymene/thymoquinone chemotype and is also found in Indian, Iranian, and Algerian black cumin seeds. Thymoquinone contents in BCO were more sensitive to the extraction temperature than hexanal and p‐cymene contents. The maximum oil yield of 36.28 ± 1.38 wt%, with approximately 2.0 mg of thymoquinone per milliliter of oil, was obtained at extraction temperatures of 50°C and pressures of 30 MPa. A SH–GC–MS could detect small molecules in black cumin seed oil such as hexanal. The results of this work reveal that temperature has more impact on the selectivity of volatile compounds than pressure in supercritical carbon dioxide extraction.

CONJUGATED LINOLEIC ACID AND FATTY ACID ISOMERS IN SELECTED COLD PRESSED OILS: ANALYSIS BY GC/FID TECHNIQUE

The conjugated linoleic acid (CLA) isomers in cold pressed oils [pomegranate seed oil (PGSO), linseed oil (LSO), black cumin seed oil (BCSO), nettle seed oil (NSO),grape seed oil (GSO), sesame seed oil (SSO),safflower oil (SFO), pumpkin seed oil (PSO), wheat germ oil (WGO), fig seed oil (FSO), coriander oil (CO), walnut oil (WO) and coconut oil (CNO)] extracted with lab–scale screw press machine were further subjected to gas chromatography/flame ionization detection (GC/FID) analysis. The composition of polyunsaturated fatty acids (PUFAs), monounsaturated fatty acids (MUFA) and saturated fatty acids (SFAs) of the samples was also determined. The five different positional and geometric isomers of CLA [cis–9, cis–11 CLA, cis–9, trans–11 CLA, trans–9, cis–11 CLA, trans–9, trans–11 CLA and trans–10, cis–12 CLA] were also well separated by a highly polar column (100m×0.2μm×0.25mm i.d; HP–88 cyanopropyl) and an applied GC temperature program. It was concluded that the samples were all rich in total CLA (∑CLA) and they were found between 0.14% for PSO and 2.11% for SSO. The most abundant CLA isomer was in general to be cis–9, trans–11 CLA form, which represented the content of isomer between 3.15% and 72.08% of ∑CLA. Besides, the ∑SFA values were detected between 2.43% and 93.14%, ∑MUFA values were between 4.60% and 71.11% and ∑PUFA values were between 1.79% and 87.59%. Therefore, this study might offer valuable information for the introduction of new food sources, as well as incorporation into medicinal purposes and food formulations which have the potential to be commercially valuable.

Nigella sativa as an antibiotic alternative to promote growth and enhance health of broilers challenged with Eimeria maxima and Clostridium perfringens

The poultry industry has significant coccidiosis and necrotic enteritis (NE) challenges, leading to high mortality and unacceptable growth without antibiotic treatment. This research explored supplementing Nigella sativa (black cumin) seed oil in poultry feed to mitigate coccidiosis and prevent or lessen NE in broilers. In vivo studies consisted of 384 and 320 Cobb 500 male broiler chicks distributed in a randomized complete block experimental design for trials 1 and 2, respectively. The first trial compared 3 concentrations (1, 2, and 5 mL/kg) of black cumin seed oil (BCSO), and trial 2 compared 2 concentrations (2 and 5 mL/kg) BCSO, with birds challenged with Eimeria maxima and Clostridium perfringens (Cp) strains Cp#6 and Cp#4, respectively. Broiler live performance, NE disease outcomes, and Cp populations were measured for both trials. A commercially available BCSO oil product, determined in a preliminary in vitro study to have the highest anti-Cp activity, was selected for in vivo studies. Gas chromatography-mass spectrometry analysis indicated the major bioactive compounds p-cymene, thymoquinone, carvacrol, and thymol were present in the BCSO. In trial 1 with strain Cp#6, BCSO concentrations of 2 and 5 mL/kg reduced NE lesion score and mortality rate to 1.6% compared with 7.8% for positive control, with no adverse impact on live performance. In trial 2 with strain Cp#4, BCSO reduced NE lesion scores and mortality rate to 35.9% compared with 51.6% for positive control and also improved weight gain when there was a Cp infection in broiler chickens. The current study compared NE in broilers challenged with 2 different Cp strains producing different levels of NE. Following Cp infection, both the population of vegetative cells and spores of Cp in cecal contents decreased for all treatments in trial 2. In conclusion, BCSO at concentrations of 2 and 5 mL/kg enhanced broiler live performance and alleviated NE and has potential as a natural, non-medication antimicrobial nutritional supplement for use as a feed additive in chickens.

Oxidative stability and compositional characteristics of oil from microwave irradiated black cumin seed under accelerated oxidation condition

The present work evaluated the impact of microwave pre-treatment on the storage stability, fatty acids and triacylglycerol contents in black cumin seed oil (BCO) during storage at 62 ºC. During storage, the oxidative indicator values (free acidity, peroxide value, p-anisidine value, TOTOX, specific extinctions and thiobarbituric acid) for the oils increased faster in untreated oil samples than in the microwaved samples. The degradation rate of polyunsaturated fatty acids (PUFAs) and triacylglycerol species (LLL and OLL) during storage were higher in untreated samples compared to treated ones, indicating that oxidation proceeded more slowly in the treated samples. During storage, the generation of hydroperoxides, their degradation and the formation of secondary oxidation products as investigated by FTIR, were lower in the treated oils. In conclusion, microwave pre-treatment prior to oil extraction reduced the oxidative degradation of oil samples, thereby increasing the storage stability of BCO.

Fabrication and Characterization of ZnO Nanoparticles-Based Biocomposite Films Prepared Using Carboxymethyl Cellulose, Taro Mucilage, and Black Cumin Seed Oil for Evaluation of Antioxidant and Antimicrobial Activities

Food packaging is often made from plastic, which is usually obtained from non-renewable resources. The development of new technologies, like biocomposite films, has been driven in response to environmental concerns as well as consumer demands for eco-friendly, high-quality products derived from nature. Biocomposite films were prepared by incorporating taro mucilage, carboxymethyl cellulose (CMC), ZnO, glycerol, and black cumin seed (BCS) oil. The SEM results showed that the biocomposite films containing taro mucilage (TM), ZnO, and BCS oil had noticeably smoother surfaces. The FTIR analysis indicated the existence of a -OH group, N-H bond, alkaline group, C-C, C=N, C-H, C-O-H, and C-O-C bond formation, confirming the interaction of CMC, glycerol, BCS oil, ZnO nanoparticles, and TM. The results of TGA and DSC analysis suggest that incorporating ZnO nanoparticles, BCS oil, and TM into the CMC polymer matrix increased thermal stability. The addition of TM significantly increased water uptake capacity, antioxidative property, tensile strength, and elongation at break, with significantly decreased whiteness index and water solubility. The film inhibited the growth of Staphylococcus aureus and Escherichia coli as foodborne pathogens. The results suggest that the films can be potentially used as environment-friendly antioxidative and antimicrobial packaging films with additional research.

Common Applications of Black Cumin Seed (Nigella sativa) Oil in Folk Medicine

Common Applications of Black Cumin Seed (Nigella sativa) Oil in Folk Medicine

Effect of ThymoQuin Black Cumin Seed Oil as a Natural Immune Modulator of Upper-Respiratory Tract Complaints and Psychological Mood State.

We conducted a randomized, double blind, placebo-controlled study to evaluate the effects of dietary supplementation with black cumin seed oil (Nigella sativa) on upper-respiratory tract complaints (URTCs) and psychological mood state. Thirty-seven runners (13.1-mile half-marathon and 26.2-mile marathon distance) consumed 500mg of black cumin seed oil extract (commercial name ThymoQuin®3%) or placebo daily during the 4 week supplementation period (3 weeks before and 1 week following a marathon or half-marathon competition). We collected subjective and objective measures before and after supplementation. Before and after supplementation, each subject completed two subjective surveys; (1) the profile of mood state (POMS) psychological assessment and (2) a standardized health log assessing health status and URTCs, as well as provided saliva samples and fecal samples for objective measurement of cortisol and microbiome balance, respectively. Compared to placebo, subjects in the ThymoQuin supplementation group reported significantly fewer upper-respiratory tract complaints (URTCs such as cough, sore throat, sinus congestion, etc) and better overall well-being (e.g. lower stress, increased energy), as well as lower stress hormones (cortisol) and superior microbiome diversity (S. thermophilus). These results suggest that ThymoQuin black cumin seed oil extract may improve immune system vigilance and overall well-being following the stress of endurance training and competition, and that these immune-mood benefits may be mediated through the Gut-Immune-Axis.

Encapsulation of black cumin seed (Nigella sativa) oil by using inverse gelation method

Black cumin seed oil (BCO) is an important oil source in the food industry. Alginate capsules with aqueous cores can be made by reversing the gelation technique with alginate and CaCl2. The research aims to investigate the physicochemical properties of the capsules as well as to analyze the storage stability of BCO emulsions (emulgators; polyglycerol polyricinoleate (A) and sorbitan monooleate (B)) coated using the inverse gelation method. The peroxide value in group A increased rapidly on the first day and then declined without any significant difference between the third and sixth days (p > 0.05). The peroxide value of group B increased significantly (p < 0.05) on the first day and then declined like in the other groups. BCO (7.42 ± 0.16) had the lowest p-anisidine value at the beginning with the initial p-anisidine value of encapsulated BCO groups A and B (21.74 ± 0.84 and 11.48 ± 1.31, respectively) having significantly higher (p < 0.05). The reverse gelation technique utilized in this study raised the p-anisidine value while lowering the peroxide value of the seed oil. The shelf life of black seed oil can be increased by using this technique in the industry.

Combination of Black Cumin Seed Oil and Astaxanthin Supports Gut-Immune-Brain-Axis and Improves Mood

This study evaluated the effects of dietary supplementation with a combination of black cumin seed oil and astaxanthin oleoresin on upper-respiratory tract complaints (URTCs) and psychological mood state.

Black cumin seed oil preparation consumption potentially improves adaptive cellular immune response among healthy volunteers

Oxidative stress and inflammatory reactions are the pathological mechanisms for most degenerative diseases. The black cumin seed oil (BCSO) contains compounds that can act as antioxidants and immunomodulators. Consuming BCSO is thought to improve antioxidant and immunomodulatory parameters in obese people. This study investigated the effect of BCSO consumption on antioxidant and immunomodulatory activity in healthy volunteers. We conducted a quasi-experimental study on 12 healthy volunteers in Yogyakarta, Indonesia. We asked the volunteers to consume BCSO for twenty days. We measured blood pressure, body mass index (BMI), malondialdehyde (MDA) level, Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvate transaminase (SGPT) activity, CD4Th, and IFN-γ expression before and after consuming BCSO. We carried out the average difference test of the parameters before and after consumption of BCSO by dependent t-test. The results showed that 3x1 BCSO preparation for 20 days reduced MDA levels and increased CDTh and IFN-γ. Consuming BCSO for 20 days potentially improve the adaptive cellular immune response parameters.

Formulation and Stability Determination of Anti-Acne Cream Containing Black Cumin Seed Oil and Kaolin Clay

Acne is the most common skin problem that could occur to any individual. Nigella sativa seed oil and kaolin as natural antimicrobial agents have been utilized in anti-acne cream formulated in this study. This study aimed at the development of anti-acne cream with antimicrobial properties using a crude extract of black cumin (Nigella sativa L.). Anti-acne creams had been formulated from cream-based agents with various percentages of crude black cumin seed extract and 1.0% (w/w) of mineral clay (Kaolin). Physical properties and stability of anti-acne cream at various storage conditions, including incubation at and freeze-thaw at 4, 40, and 45 °C for 28 days. The results showed that developed anti-acne cream containing a crude black cumin seed extract in 0.1 and 1.5% (w/w) had good physical stability. Therefore, the suitable formulation was then tested for anti-Propionibacterium acnes (P. acnes) susceptibility by the broth dilution method. From these results, it was found that 1.0 % (w/w) of crude black cumin seed extract had the ability to inhibit P.acnes with MIC (minimal inhibition concentration) of 15.6 mg/mL.

Replacement of fish oil with black soldier fly larvae oil and vegetable oils: Effects of growth, whole‐body fatty acid profile, digestive enzyme activity, haemato‐biochemical responses and muscle growth‐related gene expression of juvenile striped catfish, Pangasianodon hypophthalmus

A 10-week feeding trial was conducted to evaluate the effects of dietary replacement of fish oil (FO) with black soldier fly larvae oil (BSFLO) and vegetable oils, namely moringa oil (MO), black cumin seed oil (BCSO) and flax seed oil (FSO) on growth and whole-body fatty acid profile, digestive enzyme activity, haemato-biochemical responses and muscle growth-related gene expression of juvenile striped catfish, Pangasianodon hypophthalmus. Five isonitrogenous (313.34 g kg−1) and isolipidic (81.82 g kg−1) experimental diets were formulated. A total of 450 fingerlings (5.02 ± 0.1 g per fish) were randomly distributed into 15 tanks and fed thrice a day. The final weight gain was significantly improved in fish fed FO (45.7 ± 0.72 g/fish), BSFLO (45.53 ± 1.32 g/fish) and MO diet (47.2 ± 0.26 g/fish) when compared to BCSO (36.3 ± 1.05 g/fish) and FSO (35.83 ± 0.71g/ fish) diets. The lower value of daily weight gain was recorded in the FSO and BCSO treatments compared with other oils could be due to effects on stress resistance and immunosuppression in fish, whereas the whole-body EPA and DHA content of fish fed with FSO and FO diets was substantially higher than fish fed with other experimental diets. However, the relative expression of Myo D and Myogenin was upregulated in fish fed with FO, BSFLO and MO diet than the BCSO and FSO diet. Present results indicate that the juveniles can be reared on diets in which FO has been replaced with BSFLO and MO, with no significant effects on fish performance.

The Effect of Black Cumin Seed Oil on Active Smokers Based on Interleukin-1β Activity and Neutrophil

Health problems due to exposure to cigarettes cause the body to fight against the occurrence of immune responses, one of which is leukocytes. The most common types of leukocytes are neutrophils, which are about 50-70% with a function as the body's line of defense against foreign substances. One of the toxic components in cigarettes is nicotine. Nicotine in cigarettes can induce the production of IL-1β. Black Cumin Seed Oil (BCSO) in various studies has been widely used as a supplement, especially as an immunomodulator. This study used a single-blind Randomized Controlled Trial (RCT) method, with a total of 39 test subjects divided into four groups for 30 days. Group 1 received placebo 3x1 capsules/day, group 2 received BCSO 3x1 capsules/day, group 3 received BCSO 3x2 capsules/day, and group 4 received BCSO 3x3 capsules/day. Data analysis used a one-way ANOVA test to see the average neutrophils between treatment groups. While the average levels of IL-1β using the method of Kruskall Wallis. The test results are said to be significant if p 0.05. The p value of the ANOVA test was 0.16 neutrophil levels. In Kruskal Wallis, the –p value for IL-1β is 0.43. BCSO administration does not affect IL-1β levels in smokers and BCSO doses 3x1 capsules/day can reduce neutrophil levels.

The Effect of Black Cumin Seed Oil on Alanine Aminotransferase Levels which are Influenced by Nutritional Status in Active Smokers

Black cumin seed oil (BCSO) has the main compound thymoquinone which is thought to have hepatoprotective activity through an antioxidant mechanism. Exposure to cigarette smoke can cause cellular oxidative stress which is the key to the inflammatory process of liver cells and an increase in the activity of the Alanine aminotransferase (ALT) enzyme. Vitamin C and folic acid are nutrients that affect ALT levels through antioxidant activity. The main objective of this study was to see the effect of BCSO administration on ALT levels which were influenced by the intake of vitamin C and folic acid in healthy respondents who were active smokers for 30 days. This study used a single-blind Randomized Controlled Trial (RCT) method, with a total of 39 test subjects divided into four groups. Group 1 received 3x1 placebo, group 2 received BCSO 3x1, group 3 received BCSO 3x2, group 4 received BCSO 3x3. Investigation based on the ANOVA test, it turned out that there was no significant difference in the mean ALT levels in both the placebo group and the treatment group with p value = 0.452. For the p value of the intake of vitamin C and folic acid were 0.359 and 0.669, respectively. Based on the results of the study showed that the administration of BCSO for 30 days had no significant effect on the average activity of ALT in healthy active smokers and there was no significant difference in the intake of vitamin C and folic acid.

Pengaruh Minyak Biji Jinten Hitam (Nigella sativa L) terhadap Kadar Interleukin-4

Black cumin has a variety of efficacious substances, one of which is thymoquinon as an immunodulator, its role against Interleukin-4 with the subject of phase 2 clinical trials as an anti-inflammatory cytokine that is by inhibiting prostaglandin type E2 from arachidonic acid through induction resistance to cyclooxigenase-2. Aim to find out how the effect of Black Cumin Seed Oil in increasing levels of Interleukin-4. Randomized Clinical Trial study of 39 healthy participants of active smokers with the age of &gt;18 years. Divided into 4 groups, namely Group 1 given placebo 3x1 capsules/day, group 2,3 and 4 given Black Cumin Seed Oil 3x1,3x2 and 3x3 capsules/day given for 30 days. Percentage il-4 using ELISA method. The results were analyzed using the One Way Annova method, with consecutive p-values (0.281 and 0.860) &gt; 0.05 meaning there were meaningless differences between each treatment group. Based on the average percentage value of Interleukin-4, there was an increase in the MBJH treatment group of 3x1 capsules/day by 16,566% compared to the placebo group of 3x1 capsules/day by 13,995%.

The effect of black cumin seed oil consumption on the platelets and leukocytes number in healthy smokers in rural area Yogyakarta

Background: The high number of smokers in rural areas is a public health problem in Indonesia. Tobacco smoke exposure is associated with the incidence of cardiovascular disease and cancer. Smoking increases the activity of the hematological system, characterized by an increase in the number of platelets and leukocytes. Platelet and leukocytes are essential cells in thrombus formation and hemostasis. As an immunomodulatory supplement, black cumin seed oil (BCSO) has been used in various studies, affecting the blood cell formation system. The purpose of the study was to determine the effect of administration of BCSO preparation for 30 days on platelet and leukocytes.Methods: The 15 patients were placed into two groups in this open-label Randomized Controlled Trial. Group 1 received a placebo (3x1 capsules per day), and group 2 received BCSO (3x1 capsules per day). For 30 days, the intervention was carried out. Blood was obtained for analysis on the 31st day. The average amount of platelets and leukocytes in each group was compared using one-way ANOVA.Results: The test results are said to have no difference in platelets and leukocytes number between groups with 30 days of BCSO consumption, with their respective values being 0.848 in the placebo group and 0.211 in the treatment group.Conclusions: The homeostatic condition of subjects who consumed BCSO 3x2 capsules/day for 30 days remained stable and balanced.