The present study has demonstrated the expression of TLR1–10 genes and, HSP60 and HSP70 in peripheral blood mononuclear cells (PBMCs) during different seasons in three different age groups (Groups I, II, and III with age of 0–2, 2–5, and >5yrs, respectively) of Black Bengal goats. Real-time polymerase chain reaction was applied to investigate mRNA expression of examined factors. The study shows a wide variation in the expression of different TLR genes during different seasons and as well as in different age groups also. The mRNA expression of almost all the TLR1–10, HSP60 and HSP70 was significantly higher (p<0.05) in different age groups during peak summer season as compared to peak winter season. Moreover, lower expression of all the TLR genes except TLR3 and TLR8 was observed in winter compared to both moderate and summer season. In conclusion, results demonstrated that TLR and HSP genes could possibly play a significant role to combat the deleterious effect of thermal stress so as to maintain homeostasis in Black Bengal goats and as HSPs are the endogenous ligands of TLR2 and TLR4 thus, they play a essential modulatory role in activation of immune system in Black Bengal goat during heat stress.