Differences in distribution of minerals between the seed coat and embryo of plant seed can affect their bioavailability. Concentrations of iron [Fe], manganese [Mn], zinc [Zn], calcium [Ca], magnesium [Mg], potassium [K], and phosphorus [P] in these seed fractions of two black bean (Phaseolus vulgaris L.) cultivars (T39 and UI-911) were studied in field experiments conducted on acid and calcareous soils. Seed of T39 and UI-911 at the acid soil site had 34% and 45% higher [Fe], respectively, than at the calcareous soil site. Seed-coat [Fe] in UI-911 was substantially higher than that in T39. In contrast, embryo [Fe] was higher in T39 than UI-911. Mean percentages of the total seed Fe located in the seed coat were 33% and 32% for UI-911 at the acid and calcareous soil sites, respectively. The corresponding percentages for T39 were 14% and 18%, respectively. Although UI-911 accumulated higher percentages of Ca, Mg, and Zn in the seed coat than T39, the cultivar differences were relatively small. Mean percentages of dry matter and elements in the seed, averaged over cultivars and sites, found in the seed-coat fraction were: Dry matter=10%, Ca=84%, Fe=24%, Mg=18%, K=8%, Zn=7%, Mn=3%, and P=2%. There is apparently a genetically-controlled barrier to the movement of Fe between the seed coat and embryo during seed development in P. vulgaris.