Bivalent Metal Complexes Research Articles

Synthesis, Antimicrobial, DNA Interactions, In Vitro Cytotoxicity, and Cell Cycle Analysis Efficiency of Newly Nano‐Sized Cu (II), Ni (II), Co (II), and Zn (II) Thio‐Schiff Base Complexes of 2‐((E)‐((4,5‐Dimethyl‐2‐(((E)‐4‐Methylbenzylidene)Amino)Phenyl)imino)Methyl)‐Benzenethiol

ABSTRACTNano‐sized bivalent metal complexes, specifically [M(L)2] with L = 2‐((E)‐((4,5‐dimethyl‐2‐(((E)‐4‐methylbenzylidene)amino)phenyl)‐imino)methyl)benzenethiol] (C23H22N2S) and M = Cu (II) (C1), Co (II) (C2), Ni (II) (C3), and Zn (II) (C4), underwent synthesis and subsequent characterization. Elemental analyses, infrared, NMR, mass, electronic, magnetic susceptibility, conductivity assessments, and X‐ray diffraction studies were used to assess our bivalent metal complexes. DFT studies were used to study the tautomeric equilibrium of the tridentate thio‐Schiff base ligand (HL), via the DFT\B3LYP method in connection with a 6–311G* correlation consistent basis set. Two tautomers, which are thione and thiol forms, were studied to estimate the predominant one. The metal formed four coordinated with the tridentate N2S donor thio‐Schiff base to form octahedral geometry complexes. The SEM, TEM, XRD, AFM, and EDX of the studied complexes unveiled distinct and strong diffraction peaks, signifying their crystalline nature and providing evidence of their particle sizes being within the nano‐size. The crystal sizes calculated for all complexes were determined to be ranging from 27.73 to 76.39 nm. The interactions between metal complexes and calf thymus DNA, and their potential for mimicking insulin activity, were investigated in a controlled lab setting by measuring their ability to inhibit alpha‐amylase. The antimicrobial potential of thio‐Schiff base ligand (HL) plus complexes (C1–C4) were tested. The viscosity and UV–Vis absorption determinations were utilized to assess the calf thymus DNA (CT‐DNA) interaction with the nano‐sized metal (II) chelates. Our flow cytometry data indicate significant levels of apoptosis and cell cycle arresting in both liver and breast cancer cell lines.

Bioactive benzhydrazone derived Schiff base and its metal(II) complexes: Synthesis, characterisation, cytotoxicity, DNA binding/cleavage, antimicrobial and antioxidant activity

Bivalent mononuclear transition metal complexes of cobalt, nickel, copper and zinc were synthesized from (E)-2-(1-(2-(2-hydroxybenzoyl)hydrazono)ethoxy)benzoic acid. The FTIR, 1H NMR, 13C NMR, HR-Mass, UV–Vis., EPR, Powder XRD and SEM analysis were employed to investigate the structural features of synthesized compounds. The spectral data confirmed the square planar geometry of the complexes. The EPR spectra of the Cu(II) complex supported the proposed geometry. Powder X-ray diffraction (PXRD) analysis and scanning electron microscopy (SEM) imaging revealed that the complexes consist of nano-sized grains exhibiting a polycrystalline structure. The in vitro antimicrobial efficacy of the compounds was evaluated against various bacterial and fungal strains using the well diffusion method. The in vitro antibacterial activity demonstrated that all synthesized compounds were biologically active, and Zn(II) complex exhibited the highest inhibitory activity against S. aureus (gram-positive bacteria). For antioxidant activity, the free ligand and its metal chelates were evaluated using the standard DPPH method, revealing that the Co(II) complex displayed least inhibitory concentration value (IC50 = 16 ± 2.3 μg/mL) indicating the highest antioxidant activity. DNA interaction studies showed intercalation between complexes and DNA, and the Cu(II) complex exhibited a higher binding affinity of 2.78 ± 0.34 × 105 M−1. The DNA cleavage ability of the compounds was assessed using gel electrophoresis, demonstrating the effective cleavage of the pBR322 plasmid DNA. The Schiff base and its complexes were subjected to an anticancer evaluation against the human breast cancer cell line (MCF-7) using the MTT assay method. The percentage cell inhibition of the compounds was calculated.

Synthesis, anticancer, antibacterial, antifungal, DNA interactions, ADMET, molecular docking, and antioxidant evaluation of novel Schiff base and their Co(II), Ni(II) and Cu(II) complexes

Here, three bivalent transition metal complexes of [ML2] kind (where M = Co(II), Ni(II), Cu(II); HL = 2-(E)-(5-cyclohexyl-2-methoxyphenylimino)methyl)-5-methylphenol), and various analytical techniques such as elemental analysis, mass, IR, NMR, UV–visible, TGA, magnetic moments, and ESR were employed for characterization. From the analytical data it is found that the Ni (II) and Cu (II) complexes adopted a square planar geometry, while the Co (II) complex adopted an octahedral geometry. Molecular docking investigations were carried out of Schiff base ligand (HL), the results illustrated that the ligand exhibited significant affinities for a few proteins. Monometallic metal compounds interaction with CT-DNA was studied by fluorescence measurements, viscosity and UV–Vis investigations, which is illustrated that all the three metal compounds strongly intercalated to CT-DNA. The synthesized metal compounds (1a-1c) with supercoiled pBR322 DNA was used in cleavage experiments. Based on findings, DNA cleavage by the Cu(II) complexes was more effective than that of the Co(II) and Ni(II) compounds. All synthesised compounds toxic effects on cancer cell lines A-549 and MCF-7 were evaluated by MTT assay. The obtained results indicated that Cu(II) complex is more effective towards tumour cells than other complexes. In addition to the evaluation of all synthesised complexes for antimicrobial properties, it was observed that all of the metal complexes showed higher efficacy against microorganisms compared to the free ligand. Finally, drug likeness and ADMET studies was performed on Schiff base ligand (HL). These studies indicated that the ligand possessed high potency and showed potential as a drug agent for pathogens causing deformities.

Design of a novel -NOON- tetradentate Schiff-base scaffold supported by α-tetralone and benzothiazole moieties with its Cu2+, Co2+, and Cd2+ chelates

A novel organic moiety of Schiff base ligand, N-(benzothiazol-2-yl)-3-(2-(3,4-dihydronaphthalen-1(2H)-ylidene)hydrazinyl)-3-oxopropanamide (α-H2T) enriched with four active sites of coordination, was synthesized via the condensation of N-(benzothiazol-2-yl)-3-hydrazinyl-3-oxopropanamide with α-tetralone. This ligand was introduced to prepare a series of bivalent transition metal complexes using Cu2+, Co2+, and Cd2+ which were investigated by various spectral techniques (IR, UV–Visible, 1HNMR , 13CNMR , MS, and XRD) besides, elemental, magnetic and thermal (TG/DTA) analyses. The decomposition kinetics of metal complexes was evaluated by the use of Coats-Redfern (CR) and Horowitz-Metzger (HM) models. In addition, the DFT method was performed using (GGA/RPBE) functions and (DNP) basis set to inspect geometric optimized structures of the isolated solid compounds. Moreover, cyclic voltammetry studies were applied to all metal cations in the absence (and presence) of (α-H2T) ligand to clarify the ligation effect on the electrochemical attitude of metal ions through the shift in redox peaks potentials and the change in values of solvation and kinetic parameters. Finally, the biological studies including antimicrobial, antioxidant, cytotoxicity, and DNA binding were completed on the free ligand, metal complexes, and metal salts individually.

Preparation and Characterization of some Bivalent Metal Complexes with bis[2-(2ethyledenebenzimidazolyl)thiocarbohydrazide] and Studies of their Antiamoebic and Antihelminthic Activities

Manifestation of various medicinal potentiality and pharmacological activities associated in a pretty large number of Benzemidazole derivatives is due to presence of imidazole nuclei present in benzimidazole nuclei, which is essential constituent of Haemoglobin, Vitamin B-12, Adenine, Guanine (present in DNA). In present investigation we had prepared and characterized the complexes of some bivalent nontransition metal like Zn(II), Cd(II), Pb(II), Hg(II) and Sn(II) complexes with quadridentate ligand of benzimidazole derivative bis[2-(2ethyledenebenzimidazole) thiocarbohydrazide] (H2bisbtch) from studies of Elemental analysis, Electrical conductance data, Infrared and H-NMR spectral data. The complexes have general composition [M(bisbtch)] and almost insoluble in aqueous medium and fairly soluble in dimethyl formamide and polar organic solvent. The antiamoebic properties of ligand and its Zn(II) complexes were investigated in detail both ligand and Zn(II) complexs showed appreciable antiamoebic and antihelminthic activities similar to albendazole and Thiobendazole

Bivalent metal complexes of a novel Schiff base of vitamin B6: green synthesis, characterization, DFT studies, AIM analysis and antibacterial studies

Bivalent metal complexes of a novel Schiff base of vitamin B6: green synthesis, characterization, DFT studies, AIM analysis and antibacterial studies

Design, Synthesis, Spectral Characterization, DNA Binding and Antibacterial Studies of Ternary Metal Complexes with 1,10 Phenanthroline and 2-Acetylthiophene-4-phenyl-3-thiosemicarbazone

Bivalent metal complexes having the composition M(Phen)Cl2 (where, M = Cu(II), Ni(II) and Co(II); Phen = 1,10-Phenonthroline) are reacted with 2-acetylthiophene-4-phenyl-3-thiosemicarbazone (ATPT) to produce ternary complexes with molecular formula [M(Phen)(ATPT)Cl2] H2O. The complexes are characterized using physical (molar conductivity) and spectral (mass spectra, infrared and electronic spectroscopies) methods. Electrochemical behavior of complexes was uncovered using cyclic voltammetry. DNA binding properties of the complexes are determined by using absorption UV-Visible spectrophotometry. Metal complexes are screened for their antibacterial activity by using agar well diffusion method against pathogenic bacterial strains viz. Gram–ve such as Escherichia coli, Klebsiella Pneumonia and Gram+ve such as Staphylococcus aureus, Bacillus cereus. The [Ni (Phen)(ATPT)Cl2] 0.5 H2O complex inhibits bacteria more stronger than any other complex.
 HIGHLIGHTS
 
 Ternary Metal Complexes with 1,10 Phenanthroline and 2-Acetylthiophene-4-phenyl-3-thiosemicarbazone are synthesized
 The complexes are characterized using physico-chemical, spectral and electrochemical methods
 DNA binding properties of the complexes are determined
 Metal complexes are screened for their antibacterial activity
 The [Ni (Phen)(ATPT)Cl2] 0.5 H2O complex inhibits bacteria more effectively
 
 GRAPHICAL ABSTRACT

Bivalent metal complexes of a novel modified nicotinic acid hydrazide drug: Synthesis, characterization, and anti-tubercular studies

Iron(II) and manganese(II) complexes of N'-(1-(pyridin-2-yl)ethylidene)nicotinohydrazide (LH) have been synthesized and characterized by elemental analysis, IR, and 1H NMR spectroscopy. The crystal structure of the ligand has been determined by single crystal X-ray diffraction and electronic spectroscopic techniques. Crystal data for LH, C13H12N4O: Orthorhombic, space group Pbcn (no. 60), a = 18.0824(3) Å, b = 7.86555(14) Å, c = 16.1614(3) Å, V = 2298.60(7) Å3, Z = 8, T = 103 K, μ(Mo Kα) = 0.093 mm-1, Dcalc = 1.388 g/cm3, 36729 reflections measured (5.042° ≤ 2Θ ≤ 54.966°), 2633 unique (Rint = 0.0224, Rsigma = 0.0124) which were used in all calculations. The final R1 was 0.0383 (F2>2σ(F2)) and wR2 was 0.0988 (all data). The ligand was found to chelate to the metal ions through the azomethine nitrogen and amide oxygen atoms in a bidentate manner. The anti-tubercular activity of the ligand, its iron (II) and manganese (II) complexes were studied against Mycobacterium tuberculosis (ATTC 27294). The results revealed higher activity of the iron (II) complex with MIC value of 8.00±0.83 µM and a moderate activity of the manganese (II) complex having MIC value of 14.20±1.40 µM, compared to the reference drugs having MIC values of 9.41±0.92, 10.74±1.02, 25.34±2.6 µM and parent ligand with MIC value of 17.60±1.80 µM.

Synthesis, spectroscopic investigation, fluorescence, anticancer, antioxidant and DNA cleavage studies of coumarin hydrazone metal complexes

Bivalent transition metal complexes of manganese, cobalt, nickel, copper and zinc were synthesized from coumarin derivatives and characterized by various spectral studies. The analytical data obtained from Infrared, Nuclear Magnetic Resonance and Ultra-Violet studies confirmed that ligand binds with the metal ion via azomethine nitrogen, oxygen of the carbonyl group and the deprotonated oxygen of coumarin moiety.Thermogravimetric analysis confirmed that the presence of coordinated water molecules in the metal complexes except zinc complex.The Electron Spin Resonance spectrum showed the covalent character of copper(II) complex. The X-ray Diffraction pattern revealed the crystalline nature of nickel and manganese complexes. Fluorescence spectra of ligand and metal complexes were recorded. It is found that the fluorescence intensity of metal complexes decreased in comparison with ligand. The anticancer activity has been analyzed against the two cell lines viz.Human Breast cancer cell line (MCF-7) and Leukemia Cancer Cell Line (K-562). Studies showed that the cobalt complex has greater inhibition activity against the above two cell lines. The ligand and metal complexes were also subjected to antioxidant activities. The ligand exhibited higher free radical scavenging activity than the metal complexes. From the DNA cleavage study, it is confirmed that the nickel complex has cleaved DNA completely in the presence of Hydrogen peroxide.

Synthesis, Structure, and Antimicrobial Activity of Complexes of Bivalent Metals with 1-Naphthylacetic Acid

Synthesis, Structure, and Antimicrobial Activity of Complexes of Bivalent Metals with 1-Naphthylacetic Acid

Spectral, structural and cytotoxicity studies on the newly synthesized n'1,n'3-diisonicotinoylmalonohydrazide and some of its bivalent metal complexes

The reaction of diethyl malonate with isonicotinic hydrazide resulted in the formation of a new ligand, N'1,N'3-diisonicotinoylmalonohydrazide (H4INM), which was characterized by elemental and spectral studies. The existence of the free ligand in keto form was shown by its spectral data. The ligand reacted with Cu(II), Co(II), Ni(II) and Zn(II) acetates to produce bimetallic complexes. The ligand chelated to the metal ion in binegative tetradentate fashion via malonyl carbonyl oxygen and N2H atoms except in Zn(II) complex, where it acted as neutral tetradentate through the isonicotinyl carbonyl oxygen and N1H atoms. The various spectral measurements revealed that all complexes had an octahedral geometry except the Cu(II) complex which has square planar structure supported by the ESR spectral data. Moreover, the DFT quantum chemical calculations were performed and the HOMO-LUMO energy gap of the ligand and complexes were comparable to the optical band gap (Eg) estimated using Tauc's equation. Furthermore, isolated compounds’ in vitro cytotoxic activity against Hela (Epithelioid Carcinoma) and WISH (Human amnion) cell lines of was investigated. According to the results, the ligand exhibits moderate and weak effect against the Hela and WISH cell lines, respectively. While, the metal complexes exhibited more potent effect against WISH than against Hela, with IC50 ranging from 23.65 ± 1.7 to 46.28 ± 3.4 and 56.36 ± 3.5 to 83.88 ± 4.7 µM, respectively.

Synthesis and Spectral, Electrochemical, Protein-Docking and Biological Studies of Fluoxetine Dithiocarbamate and Its Bivalent Metal Complexes

Synthesis and Spectral, Electrochemical, Protein-Docking and Biological Studies of Fluoxetine Dithiocarbamate and Its Bivalent Metal Complexes

Spectral Characterization, DNA Binding and Antibacterial Studies of Heterolyptic Metal Complexes with 2-Acetylthiophene-4-phenyl-3-thiosemicarbazone and 2,2′-Bipyridyl

Heterolyptic metal complexes having the composition M(Bpy)Cl2 (where, M = Cu(II), Ni(II) and Co(II); Bpy = 2,2′-bipyridyl) were reacted with 2-acetylthiophene-4-phenyl-3-thiosemicarbazone (ATPT) to produce bivalent metal complexes with molecular formula M(Bpy)(ATPT)Cl·H2O. The complexes were characterized using physical (molar conductivity) and spectral (mass spectra, infrared and electronic spectroscopies) methods. Electrochemical behaviour of the complexes was revealed using cyclic voltammetry. The Cu(II)/Cu(I) couple complexes show a quasi-reversible cyclic voltammetric responses. The DNA binding properties of complexes were determined through absorption UV-visible spectrophotometry. Furthermore, the agar well diffusion method was used to screen the metal(II) complexes for their antibacterial activity against pathogenic bacterial strains, namely Gram negative strains such as Escherichia coli and Klebsiella pneumonia and Gram positve strains such as Staphylococcus aureus and Bacillus cereus. The synthesized Cu(Bpy)(ATPT)]Cl·H2O complex strongly inhibits bacteria compared with other complexes.

Antioxidant, antimicrobial, DNA binding and cleavage studies of novel Co(II), Ni(II) and Cu(II) complexes of N, O donor Schiff bases: Synthesis and spectral characterization

A series of bivalent metal complexes M(L1)2 and M(L2)2 where M = Cu(II), Ni(II), Co(II) and HL1=2-((E)-(4-(trifluoromethoxy)phenylimino)methyl)-4-chlorophenol, HL2=2-((E)-(4-(trifluoromethoxy)phenylimino)methyl)-4-nitrophenol were synthesized and characterized by elemental analysis, SEM, Mass, 1H NMR, 13C NMR, UV–Vis, FT-IR, ESR and magnetic susceptibility measurements. Based on the analytical and spectral data a square planar geometry has been assigned to all the metal complexes. DNA binding properties of these complexes have been explored using electronic absorption spectroscopy, fluorescence spectroscopy and viscosity measurements. The experimental evidence indicated that these binary complexes strongly bind to calf thymus DNA through an intercalation method. DNA cleavage efficacy of these metal(II) complexes have been investigated with super-coiled pBR322 DNA by gel electrophoresis in presence of H2O2 and UV light, and found that all complexes showed better nuclease activity. The in vitro antimicrobial activity results by paper disc method against few bacterial pathogens such as Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus as well as fungal species Aspergillus niger and Candida albicans showed that the complexes are more biocidal than free ligands. Further, the compounds were screened for antioxidant activity using 1,1-diphenyl-2-picryl hydrazyl (DPPH) which was compared with a standard drug ascorbic acid.

Heteroligand bivalent transition metal complexes with an azo-oxime ligand and 1,10-phenanthroline: Synthesis, spectroscopy, thermal analysis, DFT calculations and SOD-mimetic activities

Novel mononuclear heteroligand transition metal complexes: [M(HL)(phen)2]ClO4, (M: Mn(II) for 1, Ni(II) for 2), [M(HL)(phen)(ClO4)], (Ni(II) for 3, Cu(II) for 4, Zn(II) for 5) with 2-[(E)-(hydroxyimino)methyl]-4-[(E)-phenyldiazenyl]phenol, H2L as primary ligand and 1,10- phenanthroline as bidentate co-ligand(s) in different mole ratios have been synthesized and characterized by using elemental analysis, FTIR, UV–Vis, NMR, MALDI-TOF mass spectrometry and thermal analysis. The complexes (1, 2) have distorted octahedral geometry while the complexes (3–5) have distorted square-pyramidal coordination geometry. In the complexes, the metal ion is coordinated to the deprotonated azo-oxime ligand through the phenolic oxygen atom and nitrogen of the imine. 1,10-Phenanthroline is coordinated to the metal ion through its two N-donors. The thermograms of all the complexes were confirmed the proposed structures. Time-dependent (TD) DFT-based calculations have been also performed for geometric optimization and to assign the experimental vibrational and electronic transition of the complexes. The superoxide-scavenging activities of the complexes were also investigated and IC50 values were evaluated. Among the complexes studied, the Cu(II) complex (4) exhibits the most activity with the lowest IC50 value (2.02 ± 0.15).

Spectral, modeling and anticancer activity studies on the newly synthesized N-allyl-2-(2,4-dinitrophenyl)hydrazine-1-carbothioamide and some bivalent metal complexes

The newly synthesized ligand, N-allyl-2-(2,4-dinitrophenyl)hydrazine-1-carbothioamide, spectral structure elucidation showed that the hydrogen atom of N1H group was involved in H-bond formation with oxygen of nitro group. The reaction with acetate salt led to formation of 1:2 (M:L) complexes in case of Co(II), Ni(II) and Cd(II) while Cu(II) formed 1:1 complex. The complexes spectral data indicated that the ligand chelated in mononegative bidentate fashion via N1 and S donor atoms. All 1:2 complexes were octahedral while the Cu(II) complex was square planar as concluded from electronic and magnetic moment measurements. The DFT modeling of the ligand and complexes supported the proposed structures. The antioxidant and antitumor activity of isolated compounds were examined where the ligand and Cd(II) complex exhibited strong activities.

Synthesis, Characterization, and Antibacterial Activity of Ni(II), Pd(II), and Pt(II) Complexes with 2-benzoylthiobenzimidazole

The thioester compound of 2-benzoylthiobenzimidazole was prepared by S-acylation of 2-mercaptobenzimidazole with benzoyl chloride and reactions of divalent Ni, Pd, and Pt salts with one equivalent of thioester compound a new complexes were isolated in a high percentage. A tetradentate thioester compound and a bivalent metal complexes were identified by CHNS analysis, IR, magnetic susceptibility, molar conductivity, UV-Vis, and 1H, 13C NMR spectroscopies. The anti-bacterial activity of the thioester compound and the bivalent metal complexes were studied using disk diffusion test method against the bacterial species, Staphylococcus aureus, and Pseudomonas aeruginosa. The divalent metal complexes demonstrated a greater activities compared to thioester compound a lone. Structural analysis showed that 2-benzoylthiobenzimidazole L behaves as a neutral four coordination modes compound and bonds to the bivalent metals cation through two nitrogen, sulfur and oxygen atoms. Our study informed the formation of four-coordinate square planar complexes structure around divalent metal salts of Ni, Pd, and Pt.

Efficient synthesis, characterization, and in vitro bactericidal studies of unsymmetrically substituted triazole-derived Schiff base ligand and its transition metal complexes

In the present research, novel unsymmetrically substituted triazole-derived Schiff base ligand 2-[[[5-[[1-(5-methylfuran-2-yl)ethylidene]amino]-1H-1,2,4-triazol-3-yl]imino]methyl]phenol (L) has been synthesized by the reaction of 1,2,4-triazole-3,5-diamine with 2-hydroxybenzaldehyde and with 5-methyl-2-acetylfuran in an equimolar ratio. The synthesized ligand L was characterized by physical methods (melting point, solubility, color) as well as by spectral techniques (IR, UV–Vis, 1H NMR, 13C NMR, and MS), elemental analysis, and computational studies. Computational analysis further elucidated the structure, polarity, stability, and nature of the ligand. The bivalent(II), trivalent(III), and tetravalent(IV) transition metal complexes were formed by reacting Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II), and Cr(III) metals as chloride and, VO(IV) as sulfate with Schiff base ligand L in 1:2 (M:L) molar ratio. Ligand was coordinated with the metal ions, VO via benzaldehyde-O and azomethine-N and with Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II), and Cr(III) via benzaldehyde-O, azomethine-N, and triazole-N. The structure of the metal complexes was deduced by IR, conductance, magnetic moments, and elemental analysis. Biological evaluation of triazole-derived Schiff base ligand and its metal complexes was carried out against bacterial strains, Escherichia coli, Staphylococcus aureus, Neisseria gonorrhea, and Pseudomonas syringae. Copper complex showed the highest inhibition against all bacterial strains as compared to the free ligand and amongst other metal complexes. In vitro antibacterial studies thus concluded that the prepared ligand showed bioactivity which was enhanced upon chelation/coordination with the metal ions.

Synthesis, characterization, deoxyribonucleic acid binding and antimicrobial activity of bivalent metal complexes with 2-acetylpyridine isonicotinoylhydrazone

Synthesis, characterization, deoxyribonucleic acid binding and antimicrobial activity of bivalent metal complexes with 2-acetylpyridine isonicotinoylhydrazone

Facile synthesis, structural characterization, DNA binding, incision evaluation, antioxidant and antimicrobial activity studies of Cobalt(II), Nickle(II) and Copper(II) complexes of 3-amino-5-(4-fluorophenyl)isoxazole derivatives

Abstract A series of bivalent metal complexes [M(L1)2] and [M(L2)2] where M = Co(II), Ni(II), Cu(II) and newly synthesized Schiff bases L1 = (1-(5-(4-fluorophenyl)isoxazol-3-ylimino)methyl)napthalen-2-ol), L2 = (2-(5-(4-fluorophenyl)isoxazol-3-ylimino)methyl)-4-methylphenol) have been synthesized and characterized by elemental analysis, UV–Vis, FT-IR, Mass, NMR, ESR, magnetic moment, TGA, SEM and powder XRD analyses. Based on the spectral data, all the complexes adopted a square planar geometry around the metal ion. The interaction of metal complexes with CT–DNA was determined by using UV–absorption, fluorescence and viscosity measurements. And these results showed that the complexes moderately intercalate with CT–DNA. The DNA cleavage studies of metal complexes showed a significant cleavage of pBR322 DNA. The antioxidant activity of complexes was determined by DPPH• free radical scavenging assay. The antimicrobial activity of all the compounds was screened by disc diffusion method and it is found that the complexes showed better activity than the free ligands.