Momordica Charantia Research Articles

Characterization of Tomato Leaf Curl New Delhi Virus Associated with Leaf Curl and Yellowing Disease of Bitter Gourd from Karnataka, India

Aims: The objective of this study was to identify the ToLCNDV isolate responsible for leaf curl and yellowing disease in bitter gourd crops in Karnataka, India. Study Design: Molecular characterisation and transmission study. Place and Duration of Study: The laboratory studies were conducted in the Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore-560065. Methodology: The sample was collected and subjected to molecular characterization using specific primer for ToLCNDV and sent for sequencing. Vector and sap transmission studies were also carried out. Results: PCR-mediated detection revealed the presence of ToLCNDV, although it was not coupled with any satellite molecules. The partial genome sequencing of the AV1 gene revealed more than 97% similarity with ToLCNDV isolates. The isolate under investigation was identified as a ToLCNDV isolate and given the name ToLCNDV_GKVK_BT due to high sequence identities and phylogenetic links between the incomplete DNA-A genome and ToLCNDV isolates. The transmission experiments demonstrated that it was effectively transmitted via vector and sap transfer. Conclusion: This study identified the ToLCNDV isolate responsible for leaf curl and yellowing disease in bitter gourd crops in Karnataka, India. Identifying, characterizing at the molecular level, and understanding the transmission of begomoviruses are essential steps in developing effective management approaches to minimize agricultural losses caused by these viruses. Therefore, these findings are important for identifying the leaf curl disease of bitter gourd.

The amelioration effects of ankaferd blood stopper, platelet gel, and Momordica charantia on peripheral nerve injury in the rats: a stereological and ultrastructural study

The amelioration effects of ankaferd blood stopper, platelet gel, and Momordica charantia on peripheral nerve injury in the rats: a stereological and ultrastructural study

Effect of wild bitter gourd treatment on inflammatory responses in BALB/c mice with sepsis

Effect of wild bitter gourd treatment on inflammatory responses in BALB/c mice with sepsis

Investigation of the Influence of a Bitter Melon Product on Indicators of Cardiometabolic Health in Adults with Prediabetes

Objective The purpose of this study was to assess the impact of bitter melon extract supplementation on glycemia in individuals with prediabetes. Methods This was a 12-week randomized, parallel, placebo-controlled study where 75 adults with prediabetes were randomly allocated into the low-dose bitter melon (300 mg/day) (n = 26), high-dose bitter melon (600 mg/day) (n = 24), or control (n = 25) groups. Results At baseline and weeks 6 and 12, anthropometrics were measured, and fasting blood samples were obtained. The high-dose (1.05% ± 10.2%) and low-dose bitter melon (3.35% ± 13.2%) groups showed smaller increases in blood glucose levels at 12 wk, compared to the control group (11.0% ± 16.3%) (p < 0.05). A subgroup analysis of participants with age ≥ the median demonstrated a greater reduction in glycated hemoglobin at 12 wk in the high-dose bitter melon group (median change: −0.20%; IQRL: −0.20%, −0.05%) vs. the control group (median change: 0.00%; IQRL: −0.10%, 0.20%) (p = 0.017). Compliance with the interventions was >95%, and the study products were tolerated well. Conclusion The bitter melon extract may help maintain a healthy level of glucose in adults with prediabetes.

Response of Nutrient Spray and Pruning on Growth, Yield and Quality of Bitter Gourd (Momordica charantia L.)

A field experiment was conducted at Research Farm, Department of Agriculture (Horticulture), Faculty of Agriculture and Veterinary Sciences, Mewar University Gangrar, Chittorgarh (Rajasthan) during summer season of 2023-24 to response of nutrient spray and pruning on growth, yield and quality of bitter gourd variety “Arka Harit ‟ was used in this study. The result revealed that the both levels maximum vine length (192.29 and 187.26 cm), number of branches per plant (8.98 and 8.79), minimum appearance of first female flower (44.43 and 44.75 days) and yield parameter such as maximum number of fruit per plant (17.04 and 16.67), length of fruit (13.14 and 13.09 cm), girth of fruit (4.24 and 4.29 cm), average fruit weight (85.04 and 84.26 g), fruit yield (10.79 and 10.74 t/ha) and quality parameters such as leaf chlorophyll content (1.24 and 1.24 mg/g), TSS (2.67 and 2.65 0Brix), Vitamin C (69.20 and 68.93 mg/100 g) recorded with treatment combination of 3% spray and pruning at 9 nods. Therefore, it was concluded that the application of 3% spray and pruning at 9 nods found superior to all treatment.

STUDI KEANEKARAGAMAN OLAH TUMBUHAN BERKHASIAT OBAT DI KELURAHAN PASAR TELUKDALAM KECAMATAN TELUKDALAM

Based on the research focus, it aims to study the diversity of plants with medicinal properties in Pasar Telukdalam Village, Telukdalam District. This research aims to find out what types of medicinal plants are used by the community in Pasar Telukdalam Pasar Telukdalam Village as well as their benefits and what diseases can be cured. This type of research is qualitative with a case study approach. This research data was analyzed using data reduction, verification and drawing conclusions. The results of this research are that the types of medicinal plants used by the community in Pasar Telukdalam Village are ginger, turmeric, bitter gourd and galangal. Diseases that can be cured by medicinal plants that are used by people in Telukdalam Market Village are the ability to soothe a dry throat, overcome flatulence, overcome high stomach acid and are very good for helping to overcome coughs, shortness of breath, due to asthma, helping to relieve breathing, helps overcome nasal congestion and relieves the effects of a throbbing head due to flu. Suggestions for the community, it is hoped that this research can provide information to the community in using medicinal plants in Pasar Telukdalam Village, Telukdalam District and for Nias Raya University, it is hoped that the results of this research can enrich and add sources of information for Nias Raya University students, especially in plant processing. medicinal properties.

Biological Evaluation of Cucumis trigonus (Roxb.) for Memory Enhancing Activity in Scopolamine-induced Amnesia in Mice

Introduction: Plants have been employed for centuries to enhance human health due to their cost-effectiveness and minimal adverse effects. Numerous plants exhibit a wide spectrum of pharmacological activities, and various herbal medicines are currently being investigated for their memory-enhancing properties. Method: Cucumis trigonus (Roxb.), commonly known as Kachari or Bitter gourd, is one such plant with diverse pharmacological attributes. Historically, the fruits of C. trigonus have been utilized for addressing various conditions like leprosy, pyrexia, icterus, hyperglycemia, chronic cough, bronchitis, abdominal fluid accumulation, anemia, obstipation, diverse gastrointestinal disease, and cognitive impairment. The current investigation aims to explore the memory enhancement properties of C. trigonus, rooted in its traditional applications, particularly against scopolamine-induced amnesia in mice. Morris water maze and elevated plus maze paradigms were employed to evaluate cognitive performance. Furthermore, the research examined acetylcholine neurotransmission, oxidative stress markers, and cerebral histopathology. Result: The results showed that C. trigonus ethanol extract (dose- 150 or 300 mg/kg., p.o.), markedly (p&lt;0.05) improved memory in scopolamine-induced amnesia in mice and significantly (p&lt;0.05) elevated hippocampus acetylcholine concentration. These findings revealed that the ethanol extract of C. trigonus fruits mitigates memory deficits and demonstrates neuroprotective efficacy analogous to the reference drug Piracetam. Additionally, the extract showed notable in vivo antioxidant activity. The occurrence of flavonoids and phenols is likely to be responsible for its memory-enhancing effects due to their antioxidant properties, which help to prevent the loss of neurons. Conclusion: These results support the efficacy and potential of C. trigonus ethanol extract as an affordable alternative form of herbal medicine for the treatment of Amnesia.

Genome-Wide Analysis of the Serine Carboxypeptidase-like (SCPL) Protein Family of Bitter Gourd and Functional Validation of McSCPL22 in Fusarium oxysporum f. sp. Momordicae (FOM) Resistance.

Bitter gourd is increasingly being recognized for its value as a vegetable and medicinal use, but the molecular mechanisms of pathogen resistance remain relatively poorly understood. The serine carboxypeptidase-like (SCPL) protein family plays a key role in plant growth, pathogen defense, and so on. However, a comprehensive identification and functional characterization of the SCPL gene family has yet to be conducted in bitter melon. In this study, 32 SCPL genes were identified in bitter gourd and divided into three classes. The number of SCPL genes contained in the three clusters was 7, 7, and 18, respectively. Most SCPL gene promoters contain cis-acting elements with light, hormone, and stress responses. The RNA sequencing data showed that the expression of several SCPL genes changed significantly after pathogen infection. In particular, expression of the McSCPL4, 10, 17, 22, and 25 genes increased substantially in the resistant varieties after infection, and their expression levels were higher than those in the susceptible varieties. These results suggested that genes such as McSCPL4, 10, 17, 22, and 25 may play a significant role in conferring resistance to fungal infections. Moreover, the expression levels of the McSCPL10, 17, 22, 23, and 25 genes were likewise significantly changed after being induced by salicylic acid (SA) and jasmonic acid (JA). In situ hybridization showed that McSCPL22 was expressed in the vascular tissues of infected plants, which largely overlapped with the location of Fusarium oxysporum f. sp. Momordicae (FOM) infection and the site of hydrogen peroxide production. Our results showed that McSCPL22 may be involved in the regulation of the SA and JA pathways and enhance resistance to FOM in bitter gourd plants. This is the first study to perform SCPL gene family analysis in bitter gourd. McSCPL22 may have the potential to enhance FOM resistance in bitter gourd, and further investigation into its function is warranted. The results of this study may enhance the yield and molecular breeding of bitter gourd.

Antioxidant activities of developed natural smoothies and their effects on blood glucose levels and expression of pro-inflammatory genes in streptozotocin-induced diabetic rats

Fruits and vegetables are laden with antioxidants and have been used to quench reactive oxygen species. The combination of two or more fruits and vegetables to produce smoothies is expected to yield a synergistic effect that could further improve functionality. This study investigated the antioxidative and blood glucose-lowering potentials of smoothies made from selected fruits and vegetables as well as their effect on the expression of pro-inflammatory genes in streptozotocin-induced diabetic Wistar rats. Standard procedures were used for the in vitro antioxidant determination while pancreas tissue was excised for molecular study. The inclusion of beetroot increased the total phenolic contents by 30-50% while the flavonoid content was increased by more than 3-fold. Similarly, smoothies with beetroot and bitter gourd exhibited stronger free radical scavenging abilities as shown by DPPH (1, 1- diphenyl-2-picrylhydrazyl), ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid), Nitric oxide (NO) scavenging ability, metal chelation (Fe2+) and ferric reducing antioxidant properties (FRAP) compared to the sample devoid of the duo. The blood glucose levels of the rats fed the smoothie were significantly lowered with a similar trend in the animals that were administered metformin. The smoothies significantly (p<0.05) reduced the malondialdehyde (MDA) content thus reducing lipid peroxidation in the animals. The endogenous antioxidant enzymes; catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the pancreas were all significantly (p<0.05) increased when the animals were fed smoothies. The expression of pro-inflammatory genes, IL-1β, IL-6 and TNF-α, was down-regulated in the animals fed with smoothies. The findings showed that the smoothies made from the combination have the potential for managing type-2 diabetes mellitus.Graphical

SSR markers based QTL mapping and genetic analysis for yield and yield-attributing traits in bitter gourd (Momordica charantia L.)

The present study was conducted for QTL mapping for yield and other yield attributing traits in bitter gourd using Simple Sequence Repeat (SSR) markers. A total of 630 SSR markers were screened for polymorphism in two contrasting parents (DBGS-2 and Pusa Purvi), out of which only 35 were polymorphic. F1 plants (10 individuals) in which hybridity was ascertained (using these polymorphic markers); were further employed for development of mapping population (F2) consisting of 120 plants. Higher variation was present in the mapping population as evident from the wider range value of the characters. Continuous frequency distribution classes combined with bell-shaped, symmetrical normal distribution curve, revealed the quantitative inheritance nature of the traits studied. Higher PCV than the GCV for all the traits, along with high difference between the PCV and GCV for majority of traits indicated higher influence of environment in expression of these traits in the mapping population. Presence of transgressive segregation was also noted for majority of the traits. Amongst the various linkage groups (LG), LG 4 had the maximum number of markers, covering 171.07 cM map distance. LG4 also possessed the maximum number (nine) of QTLs while LG1 had six. QTL mapping using polymorphic SSRs resulted in detection of a total of 28 QTLs for fourteen traits viz. yield per plant (kg), earliness or flower related traits (node to first female flower, node to first male flower, days to first male flower, male to female flower ratio), fruit traits (fruit number per plant, fruit diameter (cm), fruit length/ diameter ratio, pericarp thickness (mm) and number of seed per fruit) and vegetative traits (internodal length (cm), number of primary branches, leaf width (cm), length and width ratio). The LOD score of these QTLs ranged from 3.01 to 64.47, the total phenotypic variances (PVE) ranged from 1.52 to 34.57 % and additive effects ranged from –3.69 to 17.07. Of the total, nineteen were major QTLs, having PVE >10 %. Three QTLs were detected for yield per plant while a total of seven for the traits imparting earliness viz. days to first male flower, node to first female flower and node to first male flower. Amongst all the QTLs detected, qFrtLDR-4-1 (K) had the maximum LOD (64.47) and PVE (34.57 %) value. Two hotspots were detected with multiple QTLs clustered in the LG 1. The first hotspot possessed four QTLs [qLfW-1-1, qMFR-1-1 (K), qSPF-1-1, qNFmlF-1-1 (K)] while the second had two related to yield per plant [qYldpl-1-1 (K) and qYldpl-1-1]. Many of these QTLs are also being reported for the first time in bitter gourd. The findings of the present study can be used to fasten the bitter gourd improvement by utilizing these in MAS, DNA fingerprinting, genetic mapping, genomics analysis etc.

Stability and release kinetics of nanoliposomes containing bitter melon fruit extract at different temperatures and pH values, and in simulated gastrointestinal conditions

In this study, the nanoliposomes loaded with bitter melon extract were synthesized at different concentrations of lecithin (1, 3, and 5 g/100 g) and extract (0.5 and 1 g/100 g). Thermal properties of nanoliposomes and their stability at various pH values and temperatures, as well as under simulated gastrointestinal conditions, were assessed. The results showed that an increase in the concentration of lecithin and extract elevated the melting temperature of the nanoliposomes. The release of the extract increased at high temperatures, and it was more pronounced at pH=3 than the other pH values. Also, the release followed a continuous and gradual trend under the simulated gastrointestinal conditions, slower in gastric conditions than in intestinal. Additionally, it was reduced at higher concentrations of lecithin and extract in simulated gastrointestinal conditions. The release kinetics of the extract followed different models based on temperature and pH. In conclusion, nanoliposomes were suitable and highly stable carriers for the bitter melon extract encapsulation. Moreover, the controlled release of the extract from the nanoliposomes could be properly evaluated in model systems and foodstuffs using empirical models.

The effect of ultraviolet-C on the senescence of bitter gourd fruit and the key factors analyzed by transcriptomic and metabolomic analyses

The effect of ultraviolet-C on the senescence of bitter gourd fruit and the key factors analyzed by transcriptomic and metabolomic analyses

IN SILICO ANALYSIS OF MOMORDICA CHARANTIA L. AS ANTIDIABETIC AGENTS THROUGH ACTIVATION OF HUMAN UDP-GALACTOSE 4-EPIMERASE RECEPTORS

This study investigates the antidiabetic potential of compounds found in Momordica charantia L., commonly known as bitter melon, through molecular docking and ADME (Absorption, Distribution, Metabolism, and Excretion) analysis. Utilizing in silico methods, several bioactive compounds from bitter melon, such as charantin, vicine, momordenol, momordicilin, and momordicoside, were evaluated for their ability to interact with the human UDP-Galactose 4-Epimerase receptor, a key enzyme involved in glucose metabolism. The docking results indicate that these compounds exhibit strong binding affinities, suggesting their role in glucose regulation. Further ADME analysis revealed that the compounds generally comply with the Lipinski Rule of Five, indicating favorable drug-like properties, though some compounds exhibited potential toxicities requiring further investigation. These findings highlight the potential of Momordica charantia as a source of antidiabetic agents, warranting additional in vivo and clinical studies to confirm their efficacy and safety in managing diabetes mellitus.

Medicinal Plants Approach for Diabetes Mellitus-A Computational Model

The multidimensional metabolic syndrome that includes diabetes mellitus poses a serious threat to world health. There is an increasing interest in researching herbal remedies for their possible therapeutic advantages, even as traditional allopathic treatments continue to be widely used. This work throws light on the multiple ways of metabolism and biochemical interactions of medicinal plants in the control of glucose level, highlighting their crucial role in the process. The work clarifies several herbal extracts' efficacy and safety profiles, such as Aloe vera, Garlic, Gurmar, Bitter Melon, Neem, Tulsi, and through a thorough literature review and empirical evidence. These plants, which are abundant in bioactive substances like tannins, flavonoids, and alkaloids, show promise in treating insulin resistance, improving pancreatic function, and controlling blood sugar level. A further assessment of the rising risk associated with diabetes mellitus is discussed, and a differential equation model for diabetes mellitus is developed to minimize the complications. When using medicinal plants to treat diabetes, several factors are considered, including blood sugar level, sugar intake activity, and plasma insulin concentrations. The stability criterion for the mathematical model is examined through the system of differential equations. A representation highlighting the medicinal plants that can aid individuals with diabetes mellitus is provided. The blood sugar level, insulin generalization variable and plasma insulin concentration have all been measured at different points in time. Aloe vera, Gurmar, Garlic, Tulsi, Bitter Melon and Neem are among the medicinal plants selected for their demonstrated anti-hyperglycemic properties due to their easy availability in India. Mathematical solutions were calculated for every plant and proved to be steady.

An ethnobotanical survey of medicinal plants used by diabetes patients attending the Teaching Hospital, Jaffna, Sri Lanka

The scope of this study is to describe the ethnobotany of medicinal plants used in the management of diabetes mellitus (DM) among diabetic patients attending the Teaching Hospital, Jaffna. A cross-sectional descriptive study was conducted among 388 patients attending the medical clinic of Teaching Hospital, Jaffna, using systematic sampling technique. Data were collected using a validated structured interviewer-administered questionnaire and were analysed to generate the summary of the percentages using descriptive statistics. Family Important Value (FIV) and Related Frequency Citation (RFC) were calculated to quantitatively determine the common consent between the informants on the use of endemic medicinal plants in the division. A total of 59 species belonging to 33 families were reported by 329 diabetic patients with the response rate of 100%. Among them Moringa oleifera Lamk. (142) was stated as the species with the highest RFC value, followed by Gymnema sylvestre (105), Momordica charantia Linn. (104). Moringaceae was the most dominant family with FIV 43.16. The value of RFC of the plants mentioned in this survey varied from 0.003 to 0.432. Moringa oleifera Lamk. showed the highest RFC value (0.432). In this study, 29 plant species used for DM management were reported for the first time in Sri Lanka. Further, antidiabetic property of Achyranthus polygonoides, Argyreia pomacea Wall. ex Choisy and Cassia tomentosa are yet to be investigated. These findings facilitate the documentation and the conservation of medicinal plants used for DM by the patients in Teaching Hospital, Jaffna, Sri Lanka. Moreover this study open doors for phytochemical screening and identification of new antidiabetic active compounds.

Analysis of combining ability and extent of heterosis for yield and its related traits in bitter gourd (Momordica charantia L.)

Analysis of combining ability and extent of heterosis for yield and its related traits in bitter gourd (Momordica charantia L.)

Exploring the Antidiabetic and Antihypertensive Potential of Peptides Derived from Bitter Melon Seed Hydrolysate

Background/Objectives: Type 2 diabetes (T2D) has become a critical global health issue, with an increasing prevalence that contributes to significant morbidity and mortality. Inhibiting dipeptidyl peptidase-IV (DPP4) is a promising strategy for managing T2D. This study aimed to explore the DPP4 inhibitory peptide derived from bitter melon seed protein (BMSP) hydrolysate. Methods: Reversed-phase high-performance liquid chromatography (RP-HPLC) was utilized to fractionate the hydrolysate. Peptide in the highest activity fraction was analyzed using liquid chromatography-mass spectrometry (LC-MS/MS). Peptide synthetic was used for further characterizations, such as bioactivity exploration, inhibition mechanism, molecular docking, and peptide stability against in vitro simulated gastrointestinal (SGI) digestion. Results: The BMSP hydrolysate was digested with gastrointestinal proteases (GP) and assessed for DPP4 inhibitory activity, yielding an IC50 of 1448 ± 105 μg/mL. Following RP-HPLC fractionation, MPHW (MW4) and VPSGAPF (VF7) were identified from fraction F8 with DPP4 IC50 values of 128.0 ± 1.3 µM and 150.6 ± 3.4 µM, respectively. Additionally, MW4 exhibited potential antihypertensive effects through ACE inhibition with an IC50 of 172.2 ± 10.6 µM. The inhibitory kinetics and molecular docking simulations indicated that both MW4 and VF7 were competitive inhibitors of DPP4, while MW4 was also a competitive inhibitor of ACE. Importantly, both peptides remained stable during simulated gastrointestinal digestion, suggesting their resistance to human digestive processes and their capacity to maintain biological activity. Conclusions: The findings suggest that BMSP-GP hydrolysate may have potential in terms of the development of health foods or therapeutic agents. However, in vivo studies are also essential for further confirmation of efficacy.

The Effects of Bitter Melon (Mormordica charantia) on Lipid Profile: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Research indicates that bitter melon could be useful in the management of dyslipidemia. Still, there is disagreement concerning the findings. This systematic study was undertaken to clarify the impact of consuming bitter melon on lipid profile. The databases Web of Science, Cochrane Library, PubMed, and Scopus were queried from inception until February 9, 2023. The study assessed triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) levels. The effect sizes were calculated using weighted mean differences (WMDs) and 95% confidence intervals (CIs). Eight randomized controlled trials (RCTs) with a total of 423 participants were included. Bitter melon consumption resulted in a significant decrease in plasma concentrations of TC (WMD; -9.71 mg/dL; CI: -17.69 to -1.74, p = 0.01) and TG (WMD; -10.24 mg/dL; CI: -19.92 to -0.56, p = 0.03), while bitter melon did not significantly lower blood LDL-C (WMD; -8.66 mg/dL; CI: -19.83 to 2.50, p = 0.12) and HDL-C concentrations (WMD; 0.54 mg/dL; CI: -2.38 to 3.45, p = 0.71). Subgroup analysis showed a significant decrease in TC and LDL-C and an increase in HDL-C at a dose of ≤ 2000 mg/day and an intervention period of ≤ 8 weeks. Also, the greatest impact of LDL-C and HDL-C was seen in diabetic and prediabetic people. Bitter melon supplementation positively impacts TC and TG levels. The limitations of this study were short-term trials (less than 3 months).

Formulation and evaluation of herbal gel containing ethanolic extract of Momordica charantia against socransky’s periodontal pathogens of the oral cavity-an in vitro study

Background: Periodontitis is a multifaceted disease initiated by periodontal pathogens which when left untreated results in inflammation of the supporting tissues of the teeth, resorption of alveolar bone, loss of the periodontal ligament attachment, periodontal pocket formation and/or recession of the gingiva. Hence, the current study aimed at evaluating the efficacy of Momordica charantia leaves extract on Socransky’s periodontal pathogens and formulating a herbal gel for subgingival local drug delivery for the potential treatment of moderate periodontitis. Methods: M. charantia leaves were authenticated from a recognized taxonomist. They were coarsely powdered following which an ethanol-based extract preparation was done. The extract obtained was then assessed for minimum inhibitory concentration, minimum bactericidal concentration followed by gel formulation which was then subjected to time kill assay and zone of inhibition on periodontal pathogens, the efficacy of which was comparatively evaluated against 0.12% chlorhexidine (CHX) gluconate. Kruskal-Wallis Test was employed with the statistical significance set at p≤0.05. Results: The concentration of M. charantia ethanolic extract against periodontal pathogens was determined to be 1 gm/100 ml and a statistically significant difference was found in the effectiveness between the gel so formulated and CHX. Conclusion: The antibacterial activity was evident in the ethanolic extract of M. charantia which when used to formulate a herbal gel against anaerobic periodontal pathogens demonstrated efficacy comparable to that of CHX.

Economic Analysis of kharif Vegetable Cultivation in Palghar District of Maharashtra State, India

The study was undertaken with the specific objective to workout costs, returns and profitability in vegetables production of a sample of 90 vegetable cultivators of three tahsil of Palghar viz. Vasai, Palghar and Dahanu during 2023.Simple statistical tools such as arithmetic mean, average, percentage, ratios and frequency distribution were worked out. For working out cost of production, standard cost concepts viz., cost-A1, cost-A2 cost-B1, cost-B2, cost-C1, cost-C2, cost-C3 were used. The cost of cultivation of kharif brinjal, okra, cowpea, tomato, cucumber, bottle gourd and bitter gourd was Rs.130880, Rs.172317, Rs.74835, Rs.145956, Rs.166815, Rs.220387and Rs.209361for respectively. The gross returns obtained from kharif brinjal, okra, cowpea, tomato, cucumber, bottle gourd and bitter gourd was Rs.141240, Rs.271560, Rs.77532, Rs.280280, Rs.227850, Rs.337506 and Rs.345345 respectively. The benefit-cost ratio for kharif brinjal, okra, cowpea, tomato, cucumber, bottle gourd and bitter gourd was 1.08, 1.58, 1.04, 1.92, 1.37, 1.53 and 1.65 respectively. The promising benefit-cost ratios indicate a favorable environment for vegetable farming in Palghar, suggesting that with strategic crop selection and enhanced agricultural practices, the future of vegetable cultivation in the region is likely to be both profitable and sustainable.