Garcinia Kola Research Articles

Amyloid β fibrils disruption by kolaviron: Molecular docking and extended molecular dynamics simulation studies

Garcinia kola (GK) produces notable effects against neurodegenerative conditions, including experimentally-induced Alzheimer’s disease (AD). These remarkable effects are basically attributable to kolaviron (KV), a bioflavonoid constituent of this seed. Specifically, it has been reported that in AD models, KV produces interesting neuroprotective effects, being able to diminish associated neurotoxicity, via modulation of antioxidative, inflammatory and other disease modifying processes. Intriguingly, the effect of KV on amyloid-beta (Aβ) aggregation and disruption of preformed Aβ fibrils have not been studied. In this study, we have described a thorough computational study on the mechanism of action of KV as an Aβ fibrils disruptor at molecular level. We used comprehensive in silico docking evaluations and extended molecular dynamics simulation to mimic KV/Aβ fibrils system. Results indicate that KV was able to move within the Aβ fibrils, binding with important residues and components in the Aβ peptide identified to be vital for stabilizing preformed fibrils. KV destabilized the assembled Aβ fibrils, indicating the ability KV as a potential anti-amyloidogenic agent. Furthermore, this work highlighted the possibility of identifying new multifunctional phytocompounds as potent AD drugs.

In vitro antioxidant potential and antimicrobial activity of some Cameroonian plant extracts

A study on Cameroonian edible and medicinal plants was undertaken to assess their potential as food preservatives and natural food colorants. The ultrasound-assisted technique was used for the extraction of bioactive compounds. A total of 16 extracts prepared from 7 plants (Aframomum melegueta S., Ageratum conyzoides L., Cympobogon citratus Stapf, Garcinia kola Heckel, Lantana camara L., Nauclea diderrichii, Vernonia amygdalina) were assayed to detect phytochemicals and assess antioxidant potential using the scavenging DPPH radical method and antimicrobial activity (10 mg/mL) against ten microorganisms by the agar well diffusion method. The ethanolic extract from L. camara leaves revealed the highest polyphenols and flavonoids contents. In contrast, the hexane/acetone extract from A. conyzoides and L. camara leaves had the highest chlorophyll a (Chl a) and chlorophyll b (Chl b) contents, whereas the highest carotenoid content was found in L. camara leaves. The ethanol extracts exhibited more significant antioxidant activity than hexane/acetone extracts. All seven plants (100%) inhibited the growth of at least one tested foodborne and spoilage bacteria and fungi. The germination of both Bacillus spp. spores were also affected. The hexane/acetone extracts were by far the best antimicrobial agent, and extract from L. camara leaves was one of the most effective, with a large antimicrobial spectrum. The antimicrobial activity of extracts was correlated to the amount of carotenoids and chlorophylls. HPLC-DAD revealed bioactive compounds as chlorogenic and caffeic acids found as major phenols in ethanolic extract of L. camara leaves, whereas lutein, carotene, and zeaxanthin were the specific carotenoids present in hexane/acetone extract of the same plant.

Contribution of Non-Timber Forest Products Bitter Kola, Njangsang and Palm Wine to the Income of Marketers in the Mifi Division of the West Region of Cameroon

The objective of the study is to contribute to the promotion of NTFPs (Bitter kola, Njangsang and palm wine) by assessing their contribution to the income of marketers in the Mifi division. The study was carried out in the Mifi division in the West Region of Cameroon between January and May 2020. Respondents were drawn from a cross-section of marketers. Data was collected through the administration of semi-structure questionnaires and interview guide as well as direct observations. 81 persons involved in the economic sector in five main markets were identified during the reconnaissance survey, of which 65 were interviewed accounting for 80.24%. Data analysis was carried out using descriptive statistics for processing quantitative data and to generate tables and graphs. Results revealed that every stratum of the community participates in the trade, but the main actors were of the middle age group (45 to 55 years old). Economic analysis revealed that measurement unites for NTFPs in various markets are not standardized and there is a high variation in price between period of abundance and period of scarcity. Wholesalers were able to make an annual benefit of 2 338 900 FCFA, 1 077 400 FCFA and 178 600 FCFA per person from palm wine, bitter kola and Njansang respectively. This benefit was related to the origin of products, thus, the proximity of the supply area of palm wine permitted the concerned persons to meet higher benefit. The benefit observed is this study still includes transportation cost, so in reality, the final benefit is a variable value due to the constant variation (usually little variation) of transportation cost depending on the means of transport. Nevertheless, NTFPs are of real contribution to the income of the Mifi division population, but the supply areas are out of the Mifi division and this implicates some constraints such as high transportation cost, seasonality of products and high variation of sale prices. There is a real need of domestication of species to promote their sustainable management in the actual context of climate change.

Forest Systems Services Provisioning in Africa: Case of Gambari Forest Reserve, Ibadan, Nigeria

This study, therefore, which is focused on forest systems services provisioning in Africa, case of Gambari Forest Reserve, Ibadan, Nigeria, provides policy makers, decision makers, ecologists, environmentalists, the academia, and other stakeholders with a document geared towards promoting national development through sustainable forest products utilization practices. In this study, a total of 200 key respondents participated in it, out of which 194 copies were returned and distributed among the seven main communities in the study area, namely, Ibusogbora, Oloowa, Daley north and south, Onipe, Mamu, Olubi, and Onipanu, respectively. The respondents stated that moringa 164 (84.5%), mint leaf (166 (85.6%), bitter kola 143 (73.7%), and shea tree accounts for 176 (90.7%), and the wood species utilized by producers in the study area include Leucaena leucocephala, Leucaena glauca, Gliricidia sepium, Tectona grandis, and Gmelina arborea, among others. The study recommends that there is need for African governments to restore public awareness campaign in the area of timber planting initiatives and sustainable forest resource management and increase allocation to fund forestry research in the African continent.

Amenability of priority indigenous fruit trees of West and Central Africa to grafting

ABSTRACT Grafting scions of trees with desirable features is an important step for the domestication of indigenous forest fruit trees. Two experiments were carried out in the World Agroforestry (ICRAF) experimental nursery at Yaoundé (Cameroon) to assess the graft success of five forest fruit tree species identified as priority species for domestication in West and Central Africa namely Irvingia gabonensis, Garcinia kola, Cola nitida, Ricinodendron heudelotii and Monodora myristica. In experiment 1, various grafting methods (i.e. side tongue, top cleft, side veneer, and whip-and-tongue) were tested. In experiment 2, three Irvingia species rootstocks (i.e. I. grandifolia, I. gabonensis and I. wombolu) were tested for the grafting of scions of I. gabonensis. Experiment 1 shows that the grafting method significantly affected the percentage of graft success all the tested species except G. kola where all methods resulted in a very high percentage of graft success. The top cleft grafting method had the highest percentage of success, whatever the species. Experiment 2 shows that the type of rootstock significantly influenced the graft success of I. gabonensis scions, with I. wombolu rootstocks showing the highest percentage of graft success at 45.5 ± 7.75%. Our results can guide future propagation programs for these priority fruit tree species for domestication purposes.

Garcinia kola improves cognitive and motor function of a rat model of acute radiation syndrome in the elevated plus maze.

We reported recently that the elevated plus maze is a good tool for evaluating cognitive and motor functional changes in gamma-irradiated rats as a model for new drug evaluation and monitoring. The capacity of Garcinia kola to mitigate radiation-induced brain injury is currently unknown. We therefore assessed the effects of the neuroprotective medicinal plant Garcinia kola, on the cognitive and motor changes in this murine model of acute radiation syndrome. Wistar rats exposed once to an ionizing dose of Tc99m-generated Gamma radiation were treated with an ethyl acetate fraction of methanolic extract of Garcinia kola seeds (content of 100 mg/kg of extract) for 9 weeks. Cognitive and motor function indicators were assessed in the elevated plus maze in these animals and compared with irradiated control groups (vitamin C- and vehicle-treated groups) and the non-irradiated control rats. The irradiated control group displayed cachexia, shaggy and dirty fur, porphyrin deposits around eyes, decreased exploratory activity, reduced social interactions and a loss of thigmotaxis revealed by a marked decrease in rearing episodes and stretch attend posture episodes close to the walls of elevated plus maze closed arm, an increased central platform time, and decreases in open arm time and entries. This group further displayed a decrease in head dips and grooming episodes. Treatment with Garcinia kola, and in a lesser extent vitamin C, significantly prevented the body weight loss (P < 0.001) and mitigated the development of elevated plus maze signs of cognitive and motor affections observed in the irradiated control group (P < 0.05). Altogether, our data suggest for the first time that Garcinia kola seeds have protective properties against the development of cognitive and motor decline in the acute radiation syndrome-like context. Future studies are warranted to characterize the molecular mechanisms and neuronal networks of this action.

Chemical Compositions of the Essential Oil Extracted from the Seeds of Garcina Kola, and Its Biological Activities

The essential oil was obtained from the seeds of Garcina kola and its compositions were investigated by GC-MS and ICP-MS, respectively. 74 organic compounds and 9 trace elements beneficial to human health were confirmed in this oil. Then, the in vitro antioxidant and anticancer activities were evaluated accordingly. The results showed that this essential oil exhibited stronger antioxidant activity against DPPH⸱ with the scavenging rate of 94.19% at 0.2 mg/mL, as well as potent inhibition against gastric cancer, lung cancer(A549) and Hela cell lines with the inhibitions of 96.397%±0.929, 98.005%±0.513 and 94.77±2.09 respectively at 8.3 mg/mL. While it exhibited moderate inhibition against the human breast carcinoma cells (MCF-7) with the inhibition of 59.257%±4.544 at 8.3mg/mL. In consideration of Garcina kola being consumed in Nigeria for a long time, this essential oil obtained from the Garcina kola can be used in the field of food, cosmetic or drugs.

Preliminary study on the synergistic interaction of Garcinia kola and Vernonia amygdalina against Candida albicans

Introduction: Due to the emergence and spread of infections caused by Candida albicans especially among immunocompromised patients and increase in resistance by this group of fungi to commonly used antifungal agents, this study was undertaken to investigate the possible synergy between Garcinia kola seed and Vernonia amygdalina leaves extracts against organism. Methods: Ethanol extraction of Vernonia amygdalina leaves and Garcinia kola seeds were carried out and extract evaluated for anti-candida activities. The combined anti-candida activities of the extracts were also assessed using Checkerboard assay. Results: Extract of G. kola seed showed minimal anti-candida activity against the C. albicans with an inhibition zone of 5 mm and minimum inhibitory concentration of 50 mg/mL. The combined effect of both extract was demonstrated to be synergistic at most combination ratios tested. Furthermore, the combined effect was observed to have outweighed their individual effect, inhibiting the test isolates at concentration far less than the MICs of each of the extracts. The fractional inhibitory concentration index of the combined extracts at their various combination ratios against C. albicans ranged between 0.14 – 0.45 mg/ml indicating a synergistic effect by the various combinations against the organism. Conclusion: The combined activities by the two extracts showed marked improvement in anti-candida activities compared with their individual activities. The results support harnessing the individual bioactive constituents of the extracts and combining them for the development of potential antifungal agents.

English

This study focuses on testing the efficacy of herbal remedy used in many homes within the South-Eastern part of Nigeria to treat malaria and also the histopathological effect of the remedy on albino rats. Twenty rats were procured and distributed with five rats to one group. Group 1 received Garcinia kola and Aloe vera extracts, Group 2 received only G. kola extract, Group 3 is negative control and Group 4 is positive control. The treatment lasted for 21 days but parameters were monitored on day zero, second, third, fourth and 21st day. There was a sharp weight reduction of the albino rats at the third day, Group 1 recorded 107.21&plusmn;15.46 as compared to the initial weight which was 116.18&plusmn;14.94, but later increased at day 21. The weights across the groups at day three was statistically significant (p&lt;0.05). The rats treated with G. kola extract recorded parasite density of 3997.34&plusmn;0.00 at day 21 while the group 1 had zero parasite density at day 21. This shows that G. kola extract when used with Aloe vera extract will be more effective in the treatment of malaria as to compare when used alone. Histopathological test revealed no features of acute or chronic damage, but more studies are needed.&nbsp; Key words: Malaria, Plasmodium falciparum, histopathology, Garcinia kola, Aloe vera extracts

Ameliorative effect of Kolaviron, an extract of Garcinia kola seeds, on induced hypertension.

Early diagnosis and management of known cardiovascular disease risk attributes such as hypertension lessens morbidity and mortality as well as increase quality of life of patients. This present study was modelled to investigate the ameliorative effect of Kolaviron, an extract of Garcinia kola Heckel seeds, in ethanol- and sucrose-induced hypertension. Test animals were divided into six groups of six animals each for each hypertensive model. Animals weretreated daily with distilled water (10ml/kg); 35%ethanol(3g/kg) or sucrose (5-7%); Kolaviron (50, 100 and 200 mg/kg) separately plus ethanol or sucrose and Amlodipine (0.14mg/kg) separately plus ethanol or sucrose for 8weeks. Systolic, diastolic and mean arterial pressures were determined using non-invasive BP system after 8weeks. Blood was obtained for the assessment of biochemical parameters, lipid profile and antioxidant indices. Vital organs were collected for approximation of tissue antioxidant levels. Results show that Kolaviron at various doses and Amlodipine significantly reduced (p<0.05-0.001) the elevated systolic, diastolic, and mean arterial pressures produced by ethanol and sucrose administration. Additionally, Kolaviron and Amlodipine significantly overturned (p<0.05-0.001) the reduction in GSH, SOD and CAT, and elevation in MDA levels elicited by ethanol and sucrose. Furthermore, Kolaviron and Amlodipine produced significant reduction (p<0.001) in levels of cholesterol, triglycerides and low-density lipoproteins, as well as significant increase (p<0.01-0.001) in levels of high-density lipoproteins. Results from this study demonstrate that Kolaviron possibly possesses significant antihypertensive effect which may possibly be attributed to its antioxidant effects and relative improvement of lipid profile.

Exploring the potentials of some compounds from Garcinia kola seeds towards identification of novel PDE-5 inhibitors in erectile dysfunction therapy.

Erectile dysfunction (ED) is one of the main challenges occurring among men worldwide, and is characterised by trouble getting or keeping steady erection during sexual intercourse. Various drugs like sildenafil, a phosphodiesterase-5 inhibitor (PDE-5) are freely available in the pharmacies, though normally associated with several adverse. This study was designed to assess the molecular relations obtainable between catechin, garcinal, garcinoic acid and d-tocotrienol compounds isolated from Garciniakola and targeted receptor linked to ED. These processes include the molecular docking of catechin, garcinal, garcinoic acid, d-tocotrienol, and sildenafil to receptor: PDE-5 via AutoDock Vina. Following the docking of catechin, garcinal, garcinoic acid and d-tocotrienol with the PDE-5-receptor protein, we observed that all are protein inhibitors with garcinoic acid showing better binding affinity -10.0kcal/mol with PDE-5 receptor relevant to ED. Hence, the results provided insights into the development of garcinoic acid as a replacement for present ED management, with further analysis worth considering.

Anticoccidial efficacy of Garcinia kola (Heckel H.) against experimental Eimeria tenella infection in chicks.

There is a need to advance commercial poultry production to cater to the essential protein needs of an ever-increasing population, however, the rampant occurrence of coccidiosis infection poses a threat to this achievement. This study evaluated the in vivo anticoccidial activities of the extracts and fractions of Garcinia kola against experimental Eimeria tenella infection using broiler chickens as experimental subjects. A total of 40 broiler chicks were experimentally infected with E. tenella and assigned randomly into five groups consisting of eight chicks each. Three days post experimental infection groups I and II were administered orally with tween 80 (0.8%) and Amprolium (30mg/kg) and served as untreated and treated control groups, respectively whereas Groups III, IV, and V were administered orally with crude methanol extract (CME) at doses of 200, 400 and 600mg/kg, respectively, for five consecutive days. Daily weight gains were recorded and faecal oocysts per gram (OPG) counts were made by the McMaster Egg counting technique. Blood samples from each experimental group were collected on days 0, 3, 6, and 8 for haematological examination. In the acute toxicity studies, the CME of G. kola did not produce any toxic effect or mortality at doses between 10 and 5000mg/kg. The CME G. kola was then considered safe and the LD50 was assumed to be > 5000mg/kg. Graded doses of CME of G. kola considerably (P < 0.05) improved body weight gain and decreased OPG in a dose-depended manner. There was also significant improvement in the Packed Cell Volume (PCV), Red Blood Cell (RBC) and White Blood Cell (WBC) counts upon treatment with the graded doses of CME of G. kola. Besides, G. kola significantly decreased histopathological lesions in the caecum. The results of this study indicates that G. kola may provide beneficial effects against E. tenella-induced coccidiosis in broiler chickens.

Identification of potential histone deacetylase inhibitory biflavonoids from Garcinia kola (Guttiferae) using in silico protein-ligand interaction

Abstract Overactivity of histone deacetylases (HDACs) is the underlying cause of some cancers, thus, inhibiting their overactivities is a rational treatment option. However, endeavors to employ current anti-HDACs agents in cancer treatment have yielded limited success. Consequently, there is need to explore anti-HDACs natural products, especially from plants sources, because of the intimate relationship plant products and drug discovery have enjoyed over the centuries. To identify possible HDACs inhibitors, Garcinia kola (Guttiferae) seed-derived compounds were screened in silico for HDAC-inhibitory tendencies because of their reported anticancer potentials. Fifteen G. kola-derived compounds and givinostat were docked with five selected HDACs using AutodockVina, while the binding interactions of the compounds with high binding affinities for the five HDACs were viewed with Discovery Studio Visualizer BIOVIA, 2016. Results indicated that four of the compounds studied, including amentoflavone, Garcinia biflavonoid 1, Garcinia biflavonoid 2 and kolaflavanone have higher binding propensity for all the five HDACs relative to givinostat, the standard HDAC inhibitor. This study indicated that inhibition of HDAC might be another key mechanism accountable for the bioactivities of G. kola and its intrinsic compounds. The results from this study implied that the compounds could be further investigated as drugable HDAC inhibitors with potential pharmacological applications in the treatment of cancers.

Antibacterial and Synergistic Effects of Extracts of Allium cepa, Allium sativum, Zingiber officinale and Garcinia kola on Selected Bacterial Strains

Antibacterial and Synergistic Effects of Extracts of Allium cepa, Allium sativum, Zingiber officinale and Garcinia kola on Selected Bacterial Strains

Livelihood Impacts of Forest Carbon Protection in the Context of Redd+ in Cross River State, Southeast Nigeria

The rate of landcover change linked to deforestation and forest degradation in tropical environments has continued to surge despite a series of forest governance policy instruments over the years. These informed the launch of one of the most important international policies called Reducing Emission from Deforestation and Forest Degradation Plus (REDD+) to combat forest destruction. REDD+ assumes that communities will have increased assets to natural capital which will enhance their livelihood portfolio and mitigate the effects of climate variability and change across biomes. The aim of this study is to ascertain the livelihoods impacts of forest carbon protection within the context of REDD+ in Cross River State, Nigeria. Six forest communities were chosen across three agroecological zones of the State. Anchored on the Sustainable Livelihood Framework, a set of questionnaires were administered to randomly picked households. The results indicate that more than half of the respondents aligned with financial payment and more natural resources as the perceived benefits of carbon protection. More so, a multinomial logistic regression showed that income was the main factor that influenced respondent’s support for forest carbon protection. Analysis of income trends from the ‘big seven’ non-timber forest resources in the region showed increase in Gnetum africanum, Bushmeat, Irvingia gabonensis, Garcinia kola, while carpolobia spp., Randia and rattan cane revealed declining income since inception of REDD+. The recorded increase in household income was attributed to a ban in logging. It is recommended that the forest communities should be more heavily involved in the subsequent phases of the project implementation to avoid carbon leakages.

Using decoction of some vitamin C enriched plants for the management of COVID-19 in Jos, Nigeria: A case report

The worldwide influence of coronavirus disease 2019 (COVID-19) pandemic compelled the global health organizations to prioritize the situation over all other related community health issues. The lack of specific treatment modalities against COVID-19 is a major concern nowadays. Here is a case of a 44-year-old man of 176 cm height and 87 kg body weight, who took some powdered mixture equally composed of bitter kola, cinnamon, garlic, ginger, and turmeric sweetened with three teaspoons full of honey. All ingredients were dissolved in 100 ml hot water and taken three times a day during isolation as a positive COVID-19 patient and reversed all associated signs and symptoms after 7 days as confirmed with rapid diagnostic techniques (RDTs). This case revealed that homogenized herbal tea in Jos Nigeria possesses a potential therapeutic that could be a treatment protocol against COVID-19.

Isolation and characterization of 5-hydroxymethylfurfural, antiglycation, antihyperglycaemic, antioxidant, and cytotoxic effects of Garcinia kola Heckel roots extract and fractions

Garcinia kola is a perennial medicinal plant used in ethno-medicine for the treatment of several ailments, including diabetes. The ethanol extract, dichloromethane, ethyl acetate, and butanol fractions of G. kola roots were evaluated for chemical composition, antiglycation, antidiabetic, antioxidant, and cytotoxic effects using standard procedures. 5-Hydroxymethylfurfural was isolated from the ethyl acetate fraction of G. kola roots. The chemical structure was established based on spectroscopic studies and comparison with literature data. The phytochemical test indicted high amount of flavonoids, tannins, and alkaloids in the ethanol extract, with a total phenol content in the extract (55.55 mg/g) and ethyl acetate fraction (53.55 mg/g). The extract and fractions exhibited good antiglycation activity (IC50, 0.10–0.70 mg/mL) and cytotoxicity to brine shrimps (LC50, 0.68–637.12 µg/mL). Treatment of alloxan induced diabetic rats with the ethanol extract caused a significant reduction (P < 0.05) in fasting blood glucose level (42%) in acute study and 64% reduction in prolonged treatment (2 weeks). The extract and ethyl acetate fraction also demonstrated high antioxidant effects on 2,2-diphenyl-1-picrylhydrazyl radicals (85.82 and 85.26%, respectively at 100 µg/mL) and ferric reducing power (absorbance, 0.575 and 0.559, respectively at 100 µg/mL). Garcinia kola root is a viable resource for the management of diabetes and possesses the capacity to inhibit the glycation and oxidative processes implicated in diabetic complications. The presence of 5-hydroxymethylfurfural is reported for the first time in the Garcinia genus.

English

The potted experiment was conducted in the early planting season of 2018, at the Teaching and Research Farm of Imo State University, Owerri, Nigeria to ascertain the effects of seed priming using water, coconut water and moringa leaf extract on the seedling emergence and growth of bitter kola. Completely Randomized Design was used with four treatments and three replicates. The treatments were thus; soaking of bitter kola seeds in water for 36 h, soaking of bitter kola seeds in coconut water mixed with ordinary water for 36 h, soaking of bitter kola seeds in moringa leaf extract for 36 h and non-soaked seeds (control). Bitter kola seeds were purchased from a farmer that has a bitter kola tree in his compound at Owerri, Imo State. Data were collected on number of seedlings emergence, percentage emergence, plant height, number of leaves per plant, root dry weight, shoot dry weight, leaf dry weight and total dry weight. The result revealed that priming treatments had significant effect (P&le;0.05) on number of seedlings that emerged when compared with the non-primed seeds. The least seedling emergence (0) was recorded from non-soaked seeds. Seeds soaked in water for 36 h gave the highest seedlings emergence of 10.67. Priming had effect on plant height and number of leaves. Priming also significantly affected total dry weight, shoot dry weight, leaf dry weight and root dry weight compared to the control which had no seedling that emerged. The values obtained from primed seeds were statistically similar (P&le;0.05), although water treatment gave the best result. Therefore, priming of bitter kola before planting is very necessary to enhance seedling emergence and growth. Key words: Bitter kola, priming, seedling emergence and growth, nigeria, west Africa

In-vitro antimicrobial activity of methanolic and aqueous leaf extracts of Chrysophyllum albidum (African star apple) and Garcinia kola (Bitter kola)

Methanolic and aqueous leaf extracts of Chrysophyllum albidum (African star apple) and Garcinia kola (bitter kola) were studied for in-vitro microbial activity using the disc diffusion technique. The aqueous and methanolic leaf extracts of Chrysophyllum albidum showed antimicrobial activity against Escherichia coli, Staphylococcus aureus and Candida albicans but showed no antibacterial activity against Klebsiella pneumonia. The methanolic leaf extract of Garcinia kola inhibited the growth of Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia while the aqueous extract of the leaf inhibited the growth of Staphylococcus aureus and Escherichia coli. Both methanolic and aqueous leaf extracts of the plant showed no antifungal activity against Candida albicans. The minimum inhibitory concentrations of the leaf extracts of Chrysophyllum albidum ranged from 12.5 mgL-1 to 25 mgL-1 while those of Garcinia kola ranged from 25 mgL-1 to 50 mgL-1. The results obtained suggest that the leaves of these plants can be used in treating diseases caused by the test organisms. The further investigation on the crude extracts would characterize bioactive components of the leaves of Chrysophyllum albidum and Garcinia kola.

In-vitro antimicrobial activity of methanolic and aqueous leaf extracts of Chrysophyllum albidum (African star apple) and Garcinia kola (Bitter kola)

In-vitro antimicrobial activity of methanolic and aqueous leaf extracts of Chrysophyllum albidum (African star apple) and Garcinia kola (Bitter kola)