Momordica Charantia Research Articles

First report of 'Candidatus phytoplasma asteris' (16SrI) from Cassia fistula showing symptoms of flat stem and witches'-broom in India.

Cassia fistula commonly known as 'golden shower tree' is a deciduous tree with a greenish-gray bark and complex leaves with lovely clusters of yellow blossoms that is also utilized for several purposes in traditional medicine offer therapeutic characteristics (Pawar et al., 2017). Random spotting of flat stem symptoms along with unopened flower beds was observed in C. fistula plant during March 2022 in IISER (Indian Institute of Science Education and Research), Thiruvananthapuram, Kerala, India and during May 2022 in SKUAST (Sher-e-Kashmir University of Agricultural Sciences and Technology), Jammu, which were suggestive of phytoplasma infection (Fig. 1 a-e). Surge of leaf hoppers was also observed in and around the tree. The leaf samples were collected from 3 individual C. fistula trees showing suspected symptoms of phytoplasma and one sample from asymptomatic plant of both the states. Leafhopper (LH) species were collected using sweep net method from both the locations. DNA was extracted using CTAB (Cetyl trimethyl ammonium bromide) method and nested universal PCR primers P1/P7 and R16F2n/R16R2 for the 16S rRNA gene (Deng and Hiruki 1991; Gundersen and Lee 1996) and secAfor1/secArev3 and SecAfor2/ SecArev3 for SecA gene (Hodgetts et al. 2008) were employed for the analysis of the phytoplasma strain association. The symptomatic plants and leaf hopper species showed positive bands of 1.2kb and 480bp for 16S rRNA and SecA gene respectively along with. Purified PCR products of both the genes (16Sr RNA and sec A) were ligated into pGEM ®T vector and cloned in Escherichia coli (DH5-α) were sequenced at Agri Genome labs, Kerala, India. The comparative sequence analysis using the BLASTn tool results showed 16S rRNA sequences acquired from plant samples (GenBank Acc. No. OP950857, OP950858) and the leafhoppers Hishimonus phycitis (OP538583) and Orosius albicinctus (OP538584) of Kerala had the minimum of 99.84% of similarity with Bitter gourd little leaf phytoplasma from Myanmar and maximum sequence identity (100%) with the Rapeseed phyllody phytoplasma strain from Taiwan. The sequences of phytoplasma strains from Jammu trees (Genbank Acc. No. OP801671 & OP801672) and H. phycitis (OP801673) shared 100% similarity with each other as well as with North American grapevine yellows and a minimum of 97.65% with Beta vulgaris phytoplasma from Poland. The pairwise comparison results were completely supported by the corresponding phylogenetic sequence analysis of 16S rRNA and SecA gene sequences of all the isolates in the study which clustered with 16SrI-B subgroup related strains. Virtual RFLP analysis through iPhyClassifer results that were derived from in silico digestions of R16F2n/R2 region of 16S rRNA gene using 17 restriction endonucleases enzymes indicated that all the samples produced similar virtual RFLP profiles identical to the reference strain of 16SrI-B phytoplasma subgroup (aster yellows: Acc. No. M30790) with a similarity coefficient value of 1.0. To the best of our knowledge, this is the first report of the phytoplasma association of 'Ca. P. asteris' (16SrI-B) subgroup with Cassia fistula in the world.

Genome-wide analyses of the NAC transcription factor family to reveal the potential candidate genes responding to powdery mildew in balsam pear

Genome-wide analyses of the NAC transcription factor family to reveal the potential candidate genes responding to powdery mildew in balsam pear

Interference with sex expression, estimation of yield and bioactive compounds in bitter gourd under PGRs-induced conditions

BackgroundThe bitter gourd fruit yields are not at a desirable level due to maleness. Maleness is one of the biggest difficulties with bitter gourd, reducing fruit yields substantially. On the other hand, plant stages are the most important consideration for PGR application because of their sensitive responsiveness. Therefore, it is necessary to determine how bitter gourd plants respond to plant growth regulators, namely PGR0 (control: application of water as control treatment), PGR1 (GA3-Gibberellic acid, 100 mgL−1), PGR2 (NAA-Naphthalene acetic acid, 100 mgL−1), and PGR3 (MH- Maleic hydrazide, 100 mgL−1) at three application phases, S1: soaking of seeds; S2: four-leaf; and S3: flower bud in terms of vegetative development, male–female flower sex alteration, and fruit features including bioactive compounds.ResultsPhysiological, floral and yield characteristics of bitter gourd exposed significant changes by GA3 and MH at various stages. The number of leaves plant−1, the fresh mass of the plant, and the fresh mass of fruit and fruit dry matter content were enhanced remarkably for MH application at the seed soaking stage except for plant height and the number of branches plant−1. MH increased the number of female flowers plant−1, the number of fruits plant−1 and the yield at the 4-leaf stage of application. PGRs substantially influenced the quantity of sugar, chlorophyll, total carotenoids, protein, and water content at various phases of their application but not on ascorbic acid, TSS, or total phenol concentration.ConclusionsFor sex expression and yield attributes, 100 mgL−1 MH at the 4-leaf stage would be effective in bitter gourd. Exogenous NAA showed a considerable dual influence on plant development and antioxidant enzyme activity in bitter gourd fruits.

Optimization of Magnetic and Paper-Based Molecularly Imprinted Polymers for Selective Extraction of Charantin in Momordica charantia.

Charantin is a mixture of β-sitosterol and stigmastadienol glucosides, which effectively lowers high blood glucose. Novel molecularly imprinted polymers coated magnetic nanoparticles (Fe3O4@MIPs) and filter paper (paper@MIPs) were synthesized by sol-gel polymerization to selectively extract charantin. β-sitosterol glucoside was selected as a template for imprinting a specific recognition owing to its larger molecular surface area than that of 5,25-stigmastadienol glucoside. Factorial designs were used to examine the effects of the types of porogenic solvents and cross-linkers on the extraction efficiency and imprinting factor before investigating other factors (for example, amounts of template and coated MIPs, and types of substrates for MIP immobilization). Compared to traditional liquid-liquid extraction, the optimal Fe3O4@MIP-based dispersive micro-solid phase extraction and paper@MIP extraction provided excellent extraction efficiency (87.5 ± 2.1% and 85.0 ± 2.9%, respectively) and selectivity. Charantin was well separated, and a new unidentified sterol glucoside was observed using the developed high-performance liquid chromatography with diode-array detection (Rs ≥ 2.0, n > 16,400). The developed methods were successfully utilized to extract and quantify charantin from M. charantia fruit powder and herbal products. Moreover, these methods are rapid (<10 min), inexpensive, simple, reproducible, and environmentally friendly.

Mexican Plants Involved in Glucose Homeostasis and Body Weight Control: Systematic Review

Background: Obesity is defined as abnormal or excessive fat accumulation, provoking many different diseases, such as obesity and type 2 diabetes. Type 2 diabetes is a chronic-degenerative disease characterized by increased blood glucose levels. Obesity and type 2 diabetes are currently considered public health problems, and their prevalence has increased over the last few years. Because of the high cost involved in the treatment of both diseases, different alternatives have been sought. However, the general population uses medicinal plants, in the form of tea or infusions, to treat different diseases. Therefore, traditional medicine using medicinal plants has been investigated as a possible treatment for type 2 diabetes and body weight control. Aim of the study: The purpose of this review is to find medicinal plants used in Mexico that could exert their beneficial effect by regulating insulin secretion and body weight control. Material and method: For the development of this review, Mexican plants used in traditional medicine to treat type 2 diabetes and body weight control were searched in PubMed, Google Scholar, and Scopus. The inclusion criteria include plants that presented a significant reduction in blood glucose levels and/or an increase in insulin secretion. Results: We found 306 Mexican plants with hypoglycemic effects. However, plants that did not show evidence of an increase in insulin secretion were eliminated. Finally, only five plants were included in this review: Momordica charantia L. (melón amargo), Cucurbita ficifolia bouché (chilacayote), Coriandrum sativum L. (cilantro), Persea americana Mill. (aguacate) Bidens pilosa (amor seco), including 39 articles in total. Here, we summarized the plant extracts (aqueous and organic) that have previously been reported to present hypoglycemic effects, body weight control, increased secretion and sensitivity of insulin, improvement of pancreatic β cells, and glucose tolerance. Additionally, these effects may be due to different bioactive compounds present in the plants’ extracts. Conclusion: Both in vivo and in vitro studies are required to understand the mechanism of action of these plant extracts regarding insulin secretion to be used as a possible treatment for type 2 diabetes and body weight control in the future.

Direct and Indirect Effects of Quantitative Traits on Yield of Bitter Gourd (Momordica charantia) Hybrids

The present research was conducted using 16 different hybrids of bitter gourd to study path coefficients of various yield-contributing traits and was conducted in the Department of Horticulture, Pandit Jawaharlal Nehru College of Agriculture and Research Institute, Karaikal, Puducherry, India. The results showed that the length of the vine at final harvest (10.3228) had the greatest direct positive effect on the fruit yield of vine-1, and then the days until the first fruit harvest (5.2426), flesh thickness (1.7492), the node with the first female flower (1.7188) and the number of primary branches of vine-1 in the final harvest (0.5681) and thus showed the importance of these traits in choosing a desirable bitter gourd genotype.

NILAI EKONOMI TUMBUHAN (KAJIAN ETNOMEDISIN) OLEH MASYARAKAT ADAT MANDAR DI KECAMATAN LUYO, KABUPATEN POLEWALI MANDAR

One of the economic values of plants is that they can be used as medicine. Indonesian people since ancient times have used plants as traditional medicine. This knowledge has been passed down from generation to generation and there are differences between regions. This is due to differences in customs and experiences of people who use traditional medicine. Based on this, it is necessary to carry out an inventory or record of plants that act as medicines so that they can be properly documented. The purpose of this study was to obtain data on the types of plants used as traditional medicine by the local community. This research was conducted in West Pussui Village, Luwo District, Kab. Polewali Mandar, West Sulawesi. The method used is explorative which is descriptive qualitative in nature. The data collection technique used Participatory Rural Appraisal (PRA), through in-depth interviews covering the types of plants that can be used as medicine, their uses and how to mix the plants used. In addition to interviews, direct observation was also carried out to see the conditions at the research location. The technique of selecting respondents was done by snowball sampling. Data from interviews and observations were then presented in the form of pictures and tables and then analyzed using descriptive qualitative. The results showed that the people of the Mandar tribe used kirinyuh, guava, soursop, jatropha banana, cocoa duck, papaya, black turmeric, moringa, coconut, secang wood, bitter melon, meniran, lime, purslane and breadfruit as traditional medicine to treat various kinds of diseases. The part of the plant that is most widely used to treat diseases is the leaf with a percentage of 50%.

Analysis of the Complete Mitochondrial Genome of the Bitter Gourd (Momordica charantia).

Bitter gourd (Momordica charantia L.) is a significant vegetable. Although it has a special bitter taste, it is still popular with the public. The industrialization of bitter gourd could be hampered by a lack of genetic resources. The bitter gourd's mitochondrial and chloroplast genomes have not been extensively studied. In the present study, the mitochondrial genome of bitter gourd was sequenced and assembled, and its substructure was investigated. The mitochondrial genome of bitter gourd is 331,440 bp with 24 unique core genes, 16 variable genes, 3 rRNAs, and 23 tRNAs. We identified 134 SSRs and 15 tandem repeats in the entire mitochondrial genome of bitter gourd. Moreover, 402 pairs of repeats with a length greater than or equal to 30 were observed in total. The longest palindromic repeat was 523 bp, and the longest forward repeat was 342 bp. We found 20 homologous DNA fragments in bitter gourd, and the summary insert length was 19,427 bp, accounting for 5.86% of the mitochondrial genome. We predicted a total of 447 potential RNA editing sites in 39 unique PCGs and also discovered that the ccmFN gene has been edited the most often, at 38 times. This study provides a basis for a better understanding and analysis of differences in the evolution and inheritance patterns of cucurbit mitochondrial genomes.

The FomYjeF Protein Influences the Sporulation and Virulence of Fusarium oxysporum f. sp. momordicae.

Fusarium oxysporum causes vascular wilt in more than 100 plant species, resulting in massive economic losses. A deep understanding of the mechanisms of pathogenicity and symptom induction by this fungus is necessary to control crop wilt. The YjeF protein has been proven to function in cellular metabolism damage-repair in Escherichia coli and to play an important role in Edc3 (enhancer of the mRNA decapping 3) function in Candida albicans, but no studies have been reported on related functions in plant pathogenic fungi. In this work, we report how the FomYjeF gene in F. oxysporum f. sp. momordicae contributes to conidia production and virulence. The deletion of the FomYjeF gene displayed a highly improved capacity for macroconidia production, and it was shown to be involved in carbendazim's associated stress pathway. Meanwhile, this gene caused a significant increase in virulence in bitter gourd plants with a higher disease severity index and enhanced the accumulation of glutathione peroxidase and the ability to degrade hydrogen peroxide in F. oxysporum. These findings reveal that FomYjeF affects virulence by influencing the amount of spore formation and the ROS (reactive oxygen species) pathway of F. oxysporum f. sp. momordicae. Taken together, our study shows that the FomYjeF gene affects sporulation, mycelial growth, pathogenicity, and ROS accumulation in F. oxysporum. The results of this study provide a novel insight into the function of FomYjeF participation in the pathogenicity of F. oxysporum f. sp. momordicae.

3G Cutting: An Innovative Tool in Cucurbitaceous Crops to Boost the Production and Doubling the Income of Small Farmers in a Per Unit Area

3G cutting is one of the most popular and successful inter-culture operations in cucurbitaceous crops like; bottle gourd, cucumber, pumpkin, sponge gourd, bitter gourd, etc. as well as in plants like tomato, eggplant, chilli, and lady’s finger for a small patch of land. Low fruit set is an emerging constraint in cucurbits, and when it does, extremely small-sized fruits develop and degrade the mother plant. This is the main problem that farmers are currently facing. This problem arises due to a rapid decrease in beneficial insects and other pollinators as a result of the haphazard use of foliar fertilizers and chemical pesticides. 3G cutting is a scientific process to get higher production from plants by increasing the number of female flowers in the plant by adopting practices like trimming and pruning of 1st and 2nd generation branches. Generally, 1G and 2G branches have more no. of male flowers than female (approximately in the ratio of 14:1) whereas this ratio is reduced to 1:2 in 3G branches. We are all known that fruits develop from female flowers, while one male flower can pollinate several female flowers. However, this does not imply that there is no need for male flowers. Male flowers are necessary for pollination. Thus, 3G cutting is the finest scientific practice for farmers to maximize the number of female flowers and fruit yield.

Molecular Characterization of a Bipartite Begomovirus and an Alphasatellite Associated with Leaf Curl and Mosaic Disease of Bitter Gourd in Punjab

Bitter gourd (Momordica charantia) is an economically important vegetable and medicinal plant that gets infected by a number of viruses. A survey was conducted in and around Jalandhar region of Punjab, India to study viral symptoms in the bitter gourd fields. The plants showed reduced fruit size, upward leaf curling and mosaic symptoms, indicating begomovirus infection. DNA was isolated from the symptomatic leaves and tested for begomovirus infection using group-specific PCR primers. Amplified products were cloned into pMD20-T vector and sequenced. Based on the obtained sequence, new sets of primers were designed and used for amplification of complete DNA-A and DNA-B. Complete DNA-A and DNA-B were cloned in pMD20-T and sequenced. Sequence analysis of the present DNA-A and DNA-B showed their highest identity with Tomato leaf curl New Delhi virus (ToLCNDV). The associated Tomato leaf curl New Delhi alphasatellite was amplified using universal primers, cloned and sequenced. In phylogenetic analysis, the present DNA-A and DNA-B grouped with ToLCNDV isolates and other begomoviruses reported from bitter gourd. Phylogenetic analysis of the present alphasatellite showed its closet identity with Tomato leaf curl New Delhi alphasatellite. Recombination analysis of the DNA-A sequence showed that the present bitter gourd isolate might be a recombinant of Squash leaf curl China virus (SLCChV) as a major parent and Chilli leaf curl virus as a minor parent. Analysis of the DNA-B suggested it to be a recombinant of SLCChV as a major parent and Chyaote enation yellow mosaic virus as a minor parent. The present alphasatellite might be a recombinant of Tomato yellow spot alphasatellite as a major parent and Croton yellow mosaic alphasatellite as a minor parent. The percent identities of the nucleotide and amino acid sequences validate and support the results of the phylogenetic and recombination analyses.

Integration of microRNA and mRNA analyses depicts the potential roles of Momordica charantia saponin administration in insulin resistance of juvenile common carp (Cyprinus carpio) fed with a high-starch diet.

Background: The regulation of target gene mRNA mediated by microRNA may play an important role in glucose metabolism in fish. Previous research findings of our research group revealed that Momordica charantia saponin (MS) administration in a high-starch diet could improve insulin resistance of common carp through renovating insulin signaling pathways, whose fundamental mechanisms have remained unknown by far. To reveal this potential mechanism, we aimed to investigate the difference in miRNA and mRNA expression profiles between common carp fed with high-starch diets containing MS (HS_MS1 and HS_MS2) and common carp fed with high-starch (HS) diets. Results: Through miRNA deep-sequencing, 10 significantly differentially expressed miRNAs in HC and HS_MS1, including one upregulated and nine downregulated miRNAs, were identified, whereas 10 significantly differentially expressed miRNAs in HC and HS_MS2, including four upregulated and six downregulated miRNAs, were identified. These miRNAs may not only be involved in the regulation of insulin signaling pathways and insulin resistance in common carp but also be the markers for liver insulin resistance in MS therapy for the remission of insulin resistance. This study identified 10 potential known miRNAs, namely, ccr-miR-10b, ccr-miR-122, ccr-miR-143, ccr-miR-146a, ccr-miR-155, ccr-miR-16c, ccr-miR-200a, ccr-miR-29a, ccr-miR-34, and ccr-miR-375, as candidates participating in modulating the liver insulin resistance. According to the biopathway enrichment analysis of the 252 target genes using the KEGG classical biopathway database, the relative expression levels of gsk3bb, pik3r1, and pik3r3b were analyzed using RNA-seq. Compared to the HC group, a significant decrease in the relative expression levels of pik3r1 and pik3r3b was observed in HS_MS1 and HS_MS2 groups (p < 0.05). This study raised a presumption of the presence of ccr-miR-29a targeting pik3r1 or ccr-miR-143 targeting pik3r3 playing likely roles in Momordica charantia saponins remitting the liver insulin resistance. Conclusion: The findings will further deepen the understanding of the carbohydrate metabolism of common carp and provide an important scientific basis for the application of Momordica saponins as functional nutrients to alleviate insulin resistance of fish in fish culture.

Effects of dietary wild bitter melon (Momordica charantia var. abbreviate Ser.) extract on glucose and lipid metabolism in HFD/STZ-induced type 2 diabetic rats.

Plant-based extracts to interfere with the onset of diabetes may be a promising approach towards type 2 diabetes mellitus (T2DM). Bitter gourd (Momordica charantia L.) is popularly consumed as an edible and medicinal resource with hypoglycemic effect in China. Wild bitter gourd (Momordica Charantia var. abbreviata Ser.) is a variant of bitter gourd, but there are relatively few studies on it. The purpose of the experiment is to first screen out the most effective extraction part of Momordica charantia L. and Momordica Charantia var. abbreviata Ser. through the hypoglycemic activity experiment in vitro, and by using a high-fat and high-sugar diet with STZ-induced diabetic rat model in vivo to explore the possible mechanism of action against diabetes. This study first performed α-glucosidase, PTP1B and lipase activities inhibition experiments on the alcohol and water extracts of Momordica charantia L. and Momordica Charantia var. abbreviata Ser. Sprague Dawley rats were either given normal feed or a high sugar and fat diet for four weeks, followed STZ (25 mg/kg, via i. p.) was given. Rats with fasting blood glucose ≥11.1 mmol/l after one week were deemed to be diabetic, treatments were administered for four weeks, and then blood samples were used to evaluate hematological and biochemical indicators, and liver was removed for post-analysis. The expression levels of p-AMPK, AMPK, p-PI3K, PI3K, p-AKT, AKT, p-GSK3β, GSK3β, p-IRS-1, IRS-1, GLUT2 were determined by Western blot. At the same time, the chemical components was identified by liquid-mass spectrometry. Data showed that the ethanol extract of wild bitter gourd (WBGE) had the best ability to regulate glucose and lipid metabolism in vitro. Therefore, we further investigated the antidiabetic effects of oral consumption of WBGE on high-fat diet (HFD) and streptozotocin (STZ)-induced T2DM in SD rats. WBGE effectively reduced blood glucose and lipid levels, alleviated glucose intolerance and insulin resistant. Moreover, WBGE consumption could also inhibited oxidant responses and inflammatory damage. Mechanism studies have shown that WBGE may act by regulating AMPK/PI3K signaling pathway. On the other hand, the content of total phenol, total flavonoids, total saponins and total polysaccharide were measured by UV, 27 compounds were identified by LC-MS. These studies explored the role and mechanism of WBGE in regulating glucose and lipid metabolism, and may support the utilization and further investigation of wild bitter gourd as a dietary intervention strategy to prevent diabetes and related metabolic abnormalities.

Dissipation kinetics, effect of household processing, and dietary risk assessment of the insecticide chlorantraniliprole residue in bitter gourd and soil

A single quadrupole liquid chromatography-mass spectrometry (LC-MS) method was validated to determine the insecticide chlorantraniliprole residues in bitter gourd fruit, juice, and soil, according to the SANTE guidelines. A linear curve was obtained (R2 &gt; 0.99) with LOD and LOQ at 0.003 mg kg-1 and 0.01 mg kg-1. The accuracy (87–102%) and precision (RSD &lt;5%) of the method was found to be satisfactory. The dissipation pattern of chlorantraniliprole 18.5% SC was studied by spraying twice at ten days intervals, at the recommended dose (X) (25 g a.i. ha-1), and double the recommended dose (2X) (50 g a.i. ha-1). The initial deposit on bitter gourd was 0.72 and 1.41 mg kg-1 and residues persisted up to 15 and 20 days with a half-life of 2.44 and 2.79 days at X and 2X doses, respectively. Simple decontamination techniques were found to reduce residues to the extent of 30 to 80 per cent. The reduction of chlorantraniliprole residues in bitter gourd juice was 40-50 per cent by different washing techniques. The estimated level of Risk quotient indicated (&lt;1) chlorantraniliprole residues pose no dietary risk to consumers at the level detected.

KAJIAN LITERATUR PENGARUH INSEKTISIDA NABATI DAN INSEKTISIDA SINTETIK TERHADAP KEMATIAN LARVA NYAMUK AEDES AEGYPTI

Currently, the larvicidal material that is still in great demand by the public is using synthetic insecticides. However, if it is used continuously with inappropriate doses it can cause several negative impacts such as larval resistance, environmental problems, and death of non-target organisms. Based on these negative impacts, many studies are currently examining the use of vegetable insecticides as a substitute for synthetic insecticides. This literature review aims to describe methods, review articles, and compare the results of the differences in each study related to control with vegetable insecticides and synthetic insecticides. The research method is carried out by summarizing selected journals, analyzing, and drawing conclusions. The results of this study in the literature review showed that the leaf extract of the seagrass C. serrullata was the most effective compared to the ethanol extract of bitter melon. The results of the probit analysis of the two showed that in killing 90% of the test larvae, the leaf extract of seagrass C. serrullata was able to kill at the lowest concentration (0.1675 mg/L) while the ethanol extract of bitter melon required the highest concentration (27,590 mg/L). ). Meanwhile, temephos still has the most potential in killing larvae. However, plant extracts can also potentially compete with temephos but should be at higher concentrations. Although currently temephos is still more potent than plant extracts, resistance to temephos has also been found in several areas.

Role of Fenugreek, Cinnamon, Curcuma longa, Berberine and Momordica charantia in Type 2 Diabetes Mellitus Treatment: A Review.

Type 2 diabetes mellitus (T2DM) is a complex disease that has become a major global health concern. Given the efficacy of antidiabetic drugs, pharmacological therapy is considered the first-line treatment of T2DM; however, due to their potential side effects and high costs, new and cost-effective treatments with minimal side effects are needed. Medicinal plants have been used for centuries as part of traditional medicine to treat T2DM. Among these, fenugreek, cinnamon, Curcuma longa, berberine, and Momordica charantia have demonstrated different degrees of hypoglycemic activity in clinical studies and animal models. Therefore, the aim of this review is to synthesize the mechanisms of action of five medicinal plants, as well as the experimental and clinical evidence of their hypoglycemic activity from the published literature.

Formulasi dan Karakterisasi Sediaan Spray gel Ekstrak Etanol Buah Pare (Momordica charantia L)

Bitter melon (Momordica charantia L.) fruit has been empirically used as an antibacterial and reported to have activity against acne-causing bacteria. This study aimed to formulate bitter melon ethanol extract in a spray gel preparation and test its activity against Staphylococcus epidermidis as one of the acne-causing bacteria. Pare fruit extract concentrations of 0.5%, 1%, and 2.5% are formulated into spray gel preparations with additional ingredients, Carbopol 940 0.1%, triethanolamine 0.5%, propylene glycol 15%, DMDM Hydantoin 0.6% and aquadest. The characterization of the physical properties of the spray gel preparation included organoleptic, homogeneity, pH, viscosity, and dry time test. The results showed that the spray gel preparation of ethanol extract of Pare fruit had a liquid texture, brownish-yellow colour, and distinctive odour and was homogeneous. The spray gel preparation also meets pH, viscosity and dry time requirements. The antibacterial activity of spray gel preparations resulted in a diameter response of potent and robust inhibition.

Effects of Dietary Momordica Charantia Supplementations on Broiler Performance, Blood Parameters, Meat Quality, and Intestinal Morphology

Momordica charantia (MC) is one of the most often used medicinal plants, a rich source of vitamins and minerals. This study aimed to assess the effects of a food supplementation of MC extract at various doses (0, 0.15, 0.30, 0.45 g kg-1) on the performance, carcass yield, meat quality, intestinal morphology, and some blood parameters in broilers. A total of 288 Ross 308 broiler chicks (1 day old) were used in the experiment. Animals were divided into 4 groups with 6 replicates. Feed intake (FI), daily weight gain (DWG), feed conversion rate (FCR), and carcass yield were unaffected by MC extract supplementations. The group treated with a high dosage of MC extract had significantly lower serum phosphor (P) levels than the other groups (P

Gambaran Daya Bunuh Larvasida Ekstrak Daun Pare (Momordica charantia L.) dengan Metode Infudasi dan Metode Maserasi

Culex sp. is one of the insects as a vector of chronic filariasis disease. Filariasis are often found in tropical countries such as Indonesia so its presence needs to be controlled. One of the controls that can be done is by killing the larvae with natural larvicides made by infudsion and maceration methods. This study aims to determine the description of mortality of Culex sp. larvae after being given natural larvicides, namely bitter melon leaf extract with certain concentration levels. Descriptive research was conducted with samples of Culex sp. larvae taken from a ditch which is the original habitat. This study was repeated 6 times and observation of larvae mortality at 24 hours after administration of the extract. The extract was made into 4 concentration levels, namely 1%, 5%, 10% and 15%. The four concentrations obtained results that the maceration method effectively kills 100% of Culex sp larvae at a concentration of 5% while the infusion method requires a concentration of &gt;15% so that all larvae can be killed. This can occur because in the infusion method the bioactive compounds do not diffuse optimally so that the levels of bioactive compounds as larvicides are also lower than the maceration method.

Fine-mapping and candidate gene analysis of the Mcgy1 locus responsible for gynoecy in bitter gourd (Momordica spp.).

The Mcgy1 locus responsible for gynoecy was fine-mapped into a 296.94-kb region, in which four single-nucleotide variations and six genes adjacent to them might be associate with sex differentiation in bitter gourd. Gynoecy plays an important role in high-efficiency hybrid seed production, and gynoecious plants are excellent materials for dissecting sex differentiation in Cucurbitaceae crop species, including bitter gourd. However, the gene responsible for gynoecy in bitter gourd is unknown. Here, we first identified a gynoecy locus designated Mcgy1 using the F2 population (n = 291) crossed from the gynoecious line S156G and the monoecious line K8-201 via bulked segregant analysis with whole-genome resequencing (BSA-seq) and molecular marker linkage analysis. Then, a large S156G × K8-201 F2 population (n = 5,656) was used for fine-mapping to delimit the Mcgy1 locus into a 296.94-kb physical region on pseudochromosome MC01, where included 33 annotated genes different from any homologous gynoecy genes previously reported in Cucurbitaceae species. Within this region, four underlying single-nucleotide variations (SNVs) that might cause gynoecy were identified by multiple genomic sequence variation analysis, and their six neighbouring genes were considered as potential candidate genes for Mcgy1. Of these, only MC01g1681 showed a significant differential expression at two-leaf developmental stage between S156G and its monoecious near-isogenic line S156 based on RNA sequencing (RNA-seq) and qRT-PCR analyses. In addition, transcriptome analysis revealed 21 key differentially expressed genes (DEGs) and possible regulatory pathways of the formation of gynoecy in bitter gourd. Our findings provide a new clue for researching on gynoecious plants in Cucurbitaceae species and a theoretical basis for breeding gynoecious bitter gourd lines by the use of molecular markers-assisted selection.