Spread Of H5N1 Research Articles

GIVING TEMULAWAK AND KUNYIT EXTRACT TO INCREASE PRODUCTIVITY AND AS AN AVIAN INFLUENZA IMMUNOSTIMULATOR ON BROILER CHICKEN

Broiler chickens in Indonesia have good prospects to develop. However, there are many obstacles that must be faced by broiler breeders, such as soaring prices of feed and medicine, harvest prices that tend to fluctuate, and the outbreak of bird flu that has not yet been found. The objective of the experiment was to investigate the effectiveness of treating broiler with temulawak and kunyit extracts to enhance productivity and as imunostimulator of avian influenza. Broiler were given either temulawak, kunyit or temulawak+kunyit extracts. The treatments, including a control, were arranged in a factorial design. Variables measured were production index and immune titter with haemaglutination Inhibition (HI) test at 35 days of age.Results showet that control, temulawak, kunyit and temulawak+ kunyit treated chicken have production indexes of 302,10; 387,11; 324,52; and 354,70, respectively. Haemaglutination Inhibition (HI) test result were all negative. It can be concluded that neither temulawak, kunyit or temulawak+ kunyit extracts is effective immunostimulator of avian influenza in broiler. Nevertheless, temulawak-treated chicken showed highest production index.Key Words : Temulawak, Kunyit, Avian Influenza, Haemaglutination Inhibition

Formulation of chicken egg marketing strategy

The laying chicken farming sector is an important sector because from this sector some animal protein needs for humans are met. The success of a layer farm is largely determined by marketing. Judging from the footsteps of livestock farming, it appears that older farmers have experienced several problems in animal husbandry, such as the 1997 monetary crisis, the outbreak of bird flu in late 2003, the fluctuation of corn prices in 2015. These conditions, many businesses closed farms. Even though there have been several problems with livestock, it turns out that some farms can still survive today, so it is necessary to conduct research on how to formulate an egg marketing strategy? This study took place in Karangasem Regency, Bali Province. Research data obtained from questionnaires and observations. Data analysis uses SWOT analysis techniques (strength, weakness, opportunity, and threat).

The live poultry trade and the spread of highly pathogenic avian influenza: Regional differences between Europe, West Africa, and Southeast Asia.

In the past two decades, avian influenzas have posed an increasing international threat to human and livestock health. In particular, highly pathogenic avian influenza H5N1 has spread across Asia, Africa, and Europe, leading to the deaths of millions of poultry and hundreds of people. The two main means of international spread are through migratory birds and the live poultry trade. We focus on the role played by the live poultry trade in the spread of H5N1 across three regions widely infected by the disease, which also correspond to three major trade blocs: the European Union (EU), the Economic Community of West African States (ECOWAS), and the Association of Southeast Asian Nations (ASEAN). Across all three regions, we found per-capita GDP (a proxy for modernization, general biosecurity, and value-at-risk) to be risk reducing. A more specific biosecurity measure–general surveillance–was also found to be mitigating at the all-regions level. However, there were important inter-regional differences. For the EU and ASEAN, intra-bloc live poultry imports were risk reducing while extra-bloc imports were risk increasing; for ECOWAS the reverse was true. This is likely due to the fact that while the EU and ASEAN have long-standing biosecurity standards and stringent enforcement (pursuant to the World Trade Organization’s Agreement on the Application of Sanitary and Phytosanitary Measures), ECOWAS suffered from a lack of uniform standards and lax enforcement.

A Study on the Proper Selection of Ecological Habitat for the Wild Radiation of Crested ibis(Nipponia Nippon)

This study aims to select the radiated area for the wild grazing of Crested ibis(Nipponia Nippon) in 2017 and develop a best-fit models for the wild grazing candidate area and dispersed area based on the existing restoration center in Gyeongnam area. According to the restoration policy of the endangered species Crested ibis, the regions that have a high suitability index are selected. In order to increase genetic diversity and prevent massive bird flu outbreaks, designating protection area for Crested ibis is necessary and it is recommended to release them in a geographically dispersed manner. As a result, the data obtained from the Crested ibis releasing could be shared with other restoration centers and also used as a basic data for the conservation of other endangered species in the entire Korea Peninsula.

Phylogeography of H5N1 avian influenza virus in Indonesia.

Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype are a major concern to human and animal health in Indonesia. This study aimed to characterize transmission dynamics of H5N1 over time using novel Bayesian phylogeography methods to identify factors which have influenced the spread of H5N1 in Indonesia. We used publicly available hemagglutinin sequence data sampled between 2003 and 2016 to model ancestral state reconstruction of HPAI H5N1 evolution. We found strong support for H5N1 transmission routes between provinces in Java Island and inter-island transmissions, such as between Nusa Tenggara and Kalimantan Islands, not previously described. The spread is consistent with wild bird flyways and poultry trading routes. H5N1 migration was associated with the regions of high chicken densities and low human development indices. These results can be used toinform more targeted planning of H5N1 control and prevention activities in Indonesia.

Spatial clustering of pathology submissions during the initial introduction and spread of avian influenza H5N1 in poultry in Nigeria in 2006-2007.

Highly pathogenic avian influenza (HPAI) virus H5N1 spread throughout Nigeria between 2006 and 2007. Bird samples collected across the country were submitted through the free-of-charge (FOC) program to the National Veterinary Research Institute, Vom (NVRI-Vom) laboratory. The present article describes the spatial distributions and evaluated clustering of the FOC submissions from poultry farms at the global, local, and focal levels between 2006 and 2007 epidemic in Nigeria. Spatial statistics evaluating clustering of the FOC submissions were implemented using the Moran's I test, the purely spatial cluster analysis with the SaTScan Poisson model, and the Bithell's linear score test. A significant global clustering of the FOC submissions was observed. Significant local clusters of submissions were observed in the North-East, North-Central, and South-West zones. There was significant decline in FOC submissions with increasing distance from NVRI-Vom. These results indicated that the geographic area of influence of the FOC submission program in Nigeria was limited to regions closer to the diagnostic laboratory. This work provides a detailed insight into the surveillance activities during the HPAI outbreaks in Nigeria, and should assist policy-makers and field veterinarians to improve the effectiveness of national eradication plans in the face of any outbreak of animal diseases.

Mathematical modeling of Avian Influenza epidemic with bird vaccination in constant population

The development of the industrial world and human life is increasingly modern and less attention to environmental sustainability causes the virus causes the epidemic has a high tendency to mutate so that the virus that initially only attack animals, is also found to have the ability to attack humans. The epidemics that lasted some time were bird flu epidemics and swine flu epidemics. The flu epidemic led to several deaths and many people admitted to the hospital. Strain (derivatives) of H5N1 virus was identified as the cause of the bird flu epidemic while the H1N1 strain of the virus was identified as the cause of the swine flu epidemic. The symptoms are similar to seasonal flu caused by H3N2 strain of the virus. Outbreaks of bird flu and swine flu initially only attacked animals, but over time some people were found to be infected with the virus.

Effects of Highly Pathogenic Avian Influenza H5N1 Outbreak in Nepal from Financial and Social Perspectives: A Case Study

The outbreaks of highly pathogenic avian influenza (HPAI) H5N1 have serious public health, economic and social implications. After the first two report of HPAI H5N1 outbreak in Nepal in Jhapa in 2009, a third outbreak was reported in Pokhara the following year where more than 11,000 birds were culled to control the disease. However, the financial and social effects of the Pokhara bird flu outbreak were never assessed. The objectives of this study were to evaluate the local financial and social effects of HPAI H5N1 outbreak in Pokhara and to understand farmers and consumers perceptions on bird flu and the control measures implemented by the government. A total of 200 people including poultry farmers, butchers, veterinary drug dealers, feed suppliers, and consumers were interviewed using the semi-structured questionnaire. The result showed that the direct loss of Pokhara bird flu outbreak from the culling of birds was more than 4.5 million Nepali rupees. The small scale commercial and backyard farmers and the butchers were the most affected from this outbreak. A large proportion of the consumers temporarily stopped to consume chicken meat and shifted to other meat sources. Most of the participants in this study had at least heard about the bird flu. The farmers were unhappy by the compensation provided by the government and perceived that the government control efforts were not satisfactory and were provided little assistance for their rehabilitation. In conclusion, there was a momentary local financial and social effect of the Pokhara bird flu outbreak which may not very important from the macroeconomic point of view but is important for the affected small-scale farmers and the butchers. It is recommended that the government review their policy and provide small scale commercial and backyard farmer a compensation that is closer to the market price and promote largescale poultry farmers to go for insurance.

International chicken trade and increased risk for introducing or reintroducing highly pathogenic avian influenza A (H5N1) to uninfected countries.

Every year billions of chickens are shipped thousands of miles around the globe in order to meet the ever increasing demands for this cheap and nutritious protein source. Unfortunately, transporting chickens internationally can also increase the chance for introducing zoonotic viruses, such as highly pathogenic avian influenza A (H5N1) to new countries. Our study used a retrospective analysis of poultry trading data from 2003 through 2011 to assess the risk of H5N1 poultry infection in an importing country. We found that the risk of infection in an importing country increased by a factor of 1.3 (95% CI: 1.1–1.5) for every 10-fold increase in live chickens imported from countries experiencing at least one H5N1 poultry case during that year. These results suggest that the risk in a particular country can be significantly reduced if imports from countries experiencing an outbreak are decreased during the year of infection or if biosecurity measures such as screening, vaccination, and infection control practices are increased. These findings show that limiting trade of live chickens or increasing infection control practices during contagious periods may be an important step in reducing the spread of H5N1 and other emerging avian influenza viruses.

An analysis on consumer behaviour towards meat consumption in Jammu

The present study analyses the consumers meat consumption behaviour in Jammu district of Jammu and Kashmir. After preparing the comprehensive list of meat markets operating in Jammu district, three meat markets were selected, and from each selected meat market ten retail meat shops were randomly chosen. From each randomly selected retail meat shop, a list of consumers was prepared. Out of the list four consumers were selected randomly to make a sample size of 120 consumers. Data were collected through a well structured interview schedule. The data were coded, classified, tabulated and analyzed using the software; Statistical Package for the Social Science (SPSS 16.0). The presentation of data was done to give pertinent, valid and reliable answer to the specific objectives. Frequencies, percentage, mean and standard deviation were worked out for meaningful interpretation. It was found that majority of consumers started meat consumption in early childhood and any change in the meat consumption habit was insignificant. Most of the consumers preferred chicken meat twice a week and consumption was enhanced during winter and rainy seasons while due to religious sentiments some consumers avoided consuming meat on specific days. Visual examination and fresh and disease free meat were the most prominentindicators and desired meat quality by 100% respondents.A significant proportion of respondents showed reduction in the consumption of chicken and eggs due to the fear of bird flu outbreak

Continual Antigenic Diversification in China Leads to Global Antigenic Complexity of Avian Influenza H5N1 Viruses

The highly pathogenic avian influenza (HPAI) H5N1 virus poses a significant potential threat to human society due to its wide spread and rapid evolution. In this study, we present a comprehensive antigenic map for HPAI H5N1 viruses including 218 newly sequenced isolates from diverse regions of mainland China, by computationally separating almost all HPAI H5N1 viruses into 15 major antigenic clusters (ACs) based on their hemagglutinin sequences. Phylogenetic analysis showed that 12 of these 15 ACs originated in China in a divergent pattern. Further analysis of the dissemination of HPAI H5N1 virus in China identified that the virus’s geographic expansion was co-incident with a significant divergence in antigenicity. Moreover, this antigenic diversification leads to global antigenic complexity, as typified by the recent HPAI H5N1 spread, showing extensive co-circulation and local persistence. This analysis has highlighted the challenge in H5N1 prevention and control that requires different planning strategies even inside China.

Awareness on Avian Influenza among the Workers of Selected Poultry Farms in Dhaka City

Bangladesh is a poor, densely populated country of South East Asia and vast proportion of the people here live under poverty level where poultry is emerging as a huge industry. There are about 220 million chickens and 37 million ducks in Bangladesh, millions of households rely on poultry production for income generation and nutrition. So any mishaps in the poultry industry, such as outbreak of bird flu, would have enormous economic as well as health consequences in Bangladesh. This was a cross-sectional study carried out among the poultry workers of selected poultry farms in Dhaka city. The study was conducted for a period of six months starting from January 2008 to June 2008. A total of 150 poultry workers aged 9 years and above irrespective of sexes were interviewed using semi-structured questionnaire by face to face interview. The main objective of the study was to assess the level of awareness on avian influenza among the poultry workers of selected poultry farms in Dhaka city. Data analysis revealed that 98% of the respondents ever heard avian influenza and their main source of information was television (98%). About ninety percent respondents mentioned that it is caused by virus (89.3%). Among the respondents about three fourth (68%) mentioned that it is transmitted by direct contact and 92% had the knowledge on sign and symptoms in poultry. Eighty percent of the respondents had the knowledge on transmission from poultry to human, 63% had the knowledge on transmission from human to human whereas 46.7% had the knowledge on sign & symptoms of avian influenza in human. More than eighty percent (88.7%) of the respondents believed that it can be prevented by vaccine, 56.7% believed that using personal protective equipment’s can prevent it. Though the study sample was small which might not be representative of population however, data would be useful in development of planning and program strategies.

The Evidence of Clade 7.1 Avian Influenza Virus (H5N1) in Qinghai Lake

The highly pathogenic influenza A virus subtype H5N1 spread throughout Asia since 2003, reached to Europe in 2005, and the Middle East, as well as Africa and caused a global concern for a potential pandemic threat last decade. A Clade 2.3.2 H5N1 virus became dominate in the Qinghai Lake region in 2009 with sporadic mammal cases of infection and transferred to Russia and Europe through wild migratory birds. Currently, HPAI H5N1 of clades 2.3.4, 2.3.2, and 7 are the dominant co-circulating H5N1 viruses in poultry in Asia. 2.3.2 Clade is dominant in wild birds through the world whereas there is no evident data about Clade 7 circulation in wild birds. We detected HPAI H5N1 virus of Clade 7.1 in Qinghai Lake, that closely related to Shanxi-like and Vietnam viruses co-circulating in poultry. This is the first report of Clade 7.1 H5N1 in wild birds. Based on phylogenetic analyses, the virus can be originated from Clade 7.1 virus gene pool that spread in Vietnam and Chinese poultry and could spread with migratory birds to Qinghai Lake. The Qinghai Lake continues to be significant hotspot for H5N1 surveillance since the regular outbreaks occurred there in wild birds and mammals. Based on these facts and findings, the related researchers should pay more attention to the Qinghai Lake basin as significant hotspot for H5N1 avian influenza surveillance since the regular H5N1 outbreaks occurred there in wild birds with sporadic mammal cases of infection.

Corneal Opacity in Domestic Ducks Experimentally Infected With H5N1 Highly Pathogenic Avian Influenza Virus.

Domestic ducks can be a key factor in the regional spread of H5N1 highly pathogenic avian influenza (HPAI) virus in Asia. The authors performed experimental infections to examine the relationship between corneal opacity and H5N1 HPAI virus infection in domestic ducks (Anas platyrhyncha var domestica). A total of 99 domestic ducks, including 3 control birds, were used in the study. In experiment 1, when domestic ducks were inoculated intranasally with 2 H5N1 HPAI viruses, corneal opacity appeared more frequently than neurologic signs and mortality. Corneal ulceration and exophthalmos were rare findings. Histopathologic examinations of the eyes of domestic ducks in experiment 2 revealed that corneal opacity was due to the loss of corneal endothelial cells and subsequent keratitis with edema. Influenza viral antigen was detected in corneal endothelial cells and some other ocular cells by immunohistochemistry. Results suggest that corneal opacity is a characteristic and frequent finding in domestic ducks infected with the H5N1 HPAI virus. Confirming this ocular change may improve the detection rate of infected domestic ducks in the field.

Bird flu (H7N9) outbreak and its implications on the supply chain of poultry meat in China

During the past two decades, food safety issues in China not only posed serious threats to Chinese consumers but also damaged the image of Chinese products internationally. In China, food safety is not only about scientific discoveries, advanced laboratories, and sanitation equipment; it is more about the role of different players in the food supply chain. The poultry meat supply chain is instrumental in the spread of the avian influenza A virus (H7N9), raising questions about how policymakers respond to such threats and whether industries need to be restructured to manage and control this epidemic so that it does not recur. As a short-term measure, to prevent the spread of this disease, government authorities enforced the closure of live bird markets (LBM) in disease-affected areas of China. However, in the long term, the poultry meat supply chain needs to be restructured. The aim of the current study was to analyze distribution channels for chicken meat in China and then describe arrangements in poultry meat sectors that incorporate small- and medium-scale producers into the supply chain while responding to shifts in LBMs. We also assessed the role of LBMs in spreading H7N9 and how these interventions affect the poultry meat supply chain in the Chinese market.

Ebola from emergence to epidemic: the virus and the disease, global preparedness and perspectives.

Humans constantly encounter threats from many infectious, zoonotic, and devastating pathogens. Outbreaks of severe acute respiratory syndrome (SARS), bird flu, and swine flu posing pandemic threats have compelled health agencies to follow global preparedness for combating the emerging deadly pathogens. The outbreak in West Africa of highly contagious Ebola viral disease (EVD) that started in Guinea in December 2013, assumed global proportions to become the largest outbreak of EVD and the most prominent international health concern. With fatality rates of nearly 50%-90%, it has claimed, as of 11 April 2015, 10,619 human lives out of a total of 25,626 cases reported worldwide. Ebola virus (EBOV), a member of Filoviridae family, is associated with severe, often lethal, hemorrhagic fever disease in humans and animals. The animal hosts, including non-human primates and reservoir hosts (fruit bats), play a significant role in transmission and maintenance of EBOV in nature. Although no approved vaccine for the prevention of EVD currently exists, disease control can be greatly enhanced by timely laboratory confirmation through blood tests using enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). Adherence to strict sanitary and hygienic measures, monitoring and surveillance of EBOV, as well as quarantine checks on international trade, transport, and visitors from affected countries are mandatory to prevent and control the spread of EVD. This review describes the salient properties of EBOV and the development of novel diagnostics, vaccines, and control strategies for this emerging disease of high public health concern and international emergency.

A Systematic Review of the Comparative Epidemiology of Avian and Human Influenza A H5N1 and H7N9 - Lessons and Unanswered Questions.

The aim of this work was to explore the comparative epidemiology of influenza viruses, H5N1 and H7N9, in both bird and human populations. Specifically, the article examines similarities and differences between the two viruses in their genetic characteristics, distribution patterns in human and bird populations and postulated mechanisms of global spread. In summary, H5N1 is pathogenic in birds, while H7N9 is not. Yet both have caused sporadic human cases, without evidence of sustained, human-to-human spread. The number of H7N9 human cases in the first year following its emergence far exceeded that of H5N1 over the same time frame. Despite the higher incidence of H7N9, the spatial distribution of H5N1 within a comparable time frame is considerably greater than that of H7N9, both within China and globally. The pattern of spread of H5N1 in humans and birds around the world is consistent with spread through wild bird migration and poultry trade activities. In contrast, human cases of H7N9 and isolations of H7N9 in birds and the environment have largely occurred in a number of contiguous provinces in south-eastern China. Although rates of contact with birds appear to be similar in H5N1 and H7N9 cases, there is a predominance of incidental contact reported for H7N9 as opposed to close, high-risk contact for H5N1. Despite the high number of human cases of H7N9 and the assumed transmission being from birds, the corresponding level of H7N9 virus in birds in surveillance studies has been low, particularly in poultry farms. H7N9 viruses are also diversifying at a much greater rate than H5N1 viruses. Analyses of certain H7N9 strains demonstrate similarities with engineered transmissible H5N1 viruses which make it more adaptable to the human respiratory tract. These differences in the human and bird epidemiology of H5N1 and H7N9 raise unanswered questions as to how H7N9 has spread, which should be investigated further.

Fear and Trust: How Risk Perceptions of Avian Influenza Affect the Demand for Chicken

This article quantifies the impact of H7N9 bird flu on chicken demand and consumer willingness to pay (WTP) in China. The surveys were administered to the same group of respondents in April 2012 and 2013. In 2012 we asked generic questions regarding food safety in chilled chicken without any mentioning of bird flu and in 2013 in the midst of the outbreak we resurveyed the group again. Since these respondents were surveyed both times (before and after the bird flu outbreak), the data formed a “natural experiment”. We measure risk perception, fear and trust against actual reduction in consumption and stated change in WTP for safe chicken between 2012 and 2013. Through a survey conducted in each year on the same Chinese urban consumers, we are able to construct a Marshallian-Hicksian elasticity measure signifying the relative welfare loss from a food risk. Furthermore, we found that: (1) like fear, the impact of distrust (especially the distrust in government) was negative for consumption; (2) macro fear of H7N9’s spreading, and distrust in government enhanced the deviation of consumption and WTP; (3) stronger consumer heterogeneity in consumption existed in cities with higher number of incidences; (4) the sheer mentioning of H7N9 is more important and negative than whether it was associated with a risk-perception reducing or risk-perception elevating message given to consumers; and (5) unlike what the conventional theory would predict that upon the rising of a specific risk factor, demand will drop and WTP for safter products will increase, while consumption of chicken never increased after the emergence of H7N9 in 2013, WTP for safer chicken did not increase either relative to generic risks associated with consuming chicken in 2012.

Europe's bird-flu outbreaks pose little risk to humans

Europe's bird-flu outbreaks pose little risk to humans

Biological and protective properties of immune sera directed to the influenza virus neuraminidase.

The envelope of influenza A viruses contains two large antigens, hemagglutinin (HA) and neuraminidase (NA). Conventional influenza virus vaccines induce neutralizing antibodies that are predominantly directed to the HA globular head, a domain that is subject to extensive antigenic drift. Antibodies directed to NA are induced at much lower levels, probably as a consequence of the immunodominance of the HA antigen. Although antibodies to NA may affect virus release by inhibiting the sialidase function of the glycoprotein, the antigen has been largely neglected in past vaccine design. In this study, we characterized the protective properties of monospecific immune sera that were generated by vaccination with recombinant RNA replicon particles encoding NA. These immune sera inhibited hemagglutination in an NA subtype-specific and HA subtype-independent manner and interfered with infection of MDCK cells. In addition, they inhibited the sialidase activities of various influenza viruses of the same and even different NA subtypes. With this, the anti-NA immune sera inhibited the spread of H5N1 highly pathogenic avian influenza virus and HA/NA-pseudotyped viruses in MDCK cells in a concentration-dependent manner. When chickens were immunized with NA recombinant replicon particles and subsequently infected with low-pathogenic avian influenza virus, inflammatory serum markers were significantly reduced and virus shedding was limited or eliminated. These findings suggest that NA antibodies can inhibit virus dissemination by interfering with both virus attachment and egress. Our results underline the potential of high-quality NA antibodies for controlling influenza virus replication and place emphasis on NA as a vaccine antigen. The neuraminidase of influenza A viruses is a sialidase that acts as a receptor-destroying enzyme facilitating the release of progeny virus from infected cells. Here, we demonstrate that monospecific anti-NA immune sera inhibited not only sialidase activity, but also influenza virus hemagglutination and infection of MDCK cells, suggesting that NA antibodies can interfere with virus attachment. Inhibition of both processes, virus release and virus binding, may explain why NA antibodies efficiently blocked virus dissemination in vitro and in vivo. Anti-NA immune sera showed broader reactivity than anti-HA sera in hemagglutination inhibition tests and demonstrated cross-subtype activity in sialidase inhibition tests. These remarkable features of NA antibodies highlight the importance of the NA antigen for the development of next-generation influenza virus vaccines.